(Circular) (Six-base) MAPSORT of: pFN21AB9730.seq Check: 7196 from: 1 to: 6100 pFN21AB9730 6100 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2145 2145 Size: 6100 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3443 1937 Size: 1506 4594 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4163 5547 5815 1632 Size: 2531 1384 268 1917 Fragments arranged by size: 2531 1917 1384 268 AfeI AGC'GCT Cuts at: 2924 2924 Size: 6100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2153 1184 Size: 969 5131 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3062 3094 3062 Size: 32 6068 AlwNI CAG_nnn'CTG Cuts at: 2338 2425 3268 3843 5415 2338 Size: 87 843 575 1572 3023 Fragments arranged by size: 3023 1572 843 575 87 ApaI G_GGCC'C Cuts at: 2227 2227 Size: 6100 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3409 3635 3787 5896 1493 Size: 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2611 2808 3425 1870 Size: 73 668 197 617 4545 Fragments arranged by size: 4545 668 617 197 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2728 3421 4876 618 Size: 325 217 627 278 663 693 1455 1842 Fragments arranged by size: 1842 1455 693 663 627 325 278 217 BanII G_rGCy'C Cuts at: 1391 2227 2595 2817 2919 3419 1391 Size: 836 368 222 102 500 4072 Fragments arranged by size: 4072 836 500 368 222 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3169 928 Size: 971 1270 3859 Fragments arranged by size: 3859 1270 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2244 2430 4711 Size: 107 122 71 631 605 572 186 2281 Cuts at: 4711 136 Size: 1525 Fragments arranged by size: 2281 1525 631 605 572 186 122 107 71 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 3103 3281 3517 3103 Size: 178 236 5686 Fragments arranged by size: 5686 236 178 Bme1580I G_kGCm'C Cuts at: 1790 2070 2227 2733 2804 4161 5317 1790 Size: 280 157 506 71 1357 1156 2573 Fragments arranged by size: 2573 1357 1156 506 280 157 71 BmgBI CAC'GTC Cuts at: 1318 2518 1318 Size: 1200 4900 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3831 4789 442 Size: 416 53 83 399 24 2414 958 1753 Fragments arranged by size: 2414 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3367 3367 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2536 3034 4760 1239 Size: 1297 498 1726 2579 Fragments arranged by size: 2579 1726 1297 498 Bpu10I CC'TnA_GC Cuts at: 2229 2229 Size: 6100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2903 4225 5090 5388 2903 Size: 1322 865 298 3615 Fragments arranged by size: 3615 1322 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3032 4763 882 Size: 617 1533 1731 2219 Fragments arranged by size: 2219 1731 1533 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2171 3907 4289 4924 Size: 53 83 186 936 638 1736 382 635 Cuts at: 4924 275 Size: 1451 Fragments arranged by size: 1736 1451 936 638 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2558 3045 4533 4931 5205 5352 5763 2558 Size: 487 1488 398 274 147 411 2895 Fragments arranged by size: 2895 1488 487 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2141 2171 3062 3092 1178 Size: 30 165 30 738 30 891 30 4186 Fragments arranged by size: 4186 891 738 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3334 1186 Size: 2148 3952 BseYI C'CCAG_C Cuts at: 2086 2583 3261 3268 5303 5799 2086 Size: 497 678 7 2035 496 2387 Fragments arranged by size: 2387 2035 678 497 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2027 1163 Size: 573 291 5236 Fragments arranged by size: 5236 573 291 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 2686 2721 3034 3550 4311 Size: 275 186 33 652 35 313 516 761 Cuts at: 4311 5339 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 652 516 313 275 186 35 33 BsiHKAI G_wGCw'C Cuts at: 2235 2919 2964 3287 4161 4246 5317 2235 Size: 684 45 323 874 85 1071 3018 Fragments arranged by size: 3018 1071 874 684 323 85 45 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2227 2235 2595 2733 2804 2817 Size: 399 280 157 8 360 138 71 13 Cuts at: 2817 2919 2964 3287 3419 4161 4246 5317 1391 Size: 102 45 323 132 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 323 280 157 138 132 102 85 71 45 13 8 BspEI T'CCGG_A Cuts at: 3045 5763 3045 Size: 2718 3382 BspHI T'CATG_A Cuts at: 3303 3989 5719 3303 Size: 686 1730 3684 Fragments arranged by size: 3684 1730 686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2091 2145 2830 3000 3455 844 Size: 1247 54 685 170 455 3489 Fragments arranged by size: 3489 1247 685 455 170 54 BsrBI CCG'CTC Cuts at: 1394 1823 2442 2970 3987 1394 Size: 429 619 528 1017 3507 Fragments arranged by size: 3507 1017 619 528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2854 4595 4769 65 Size: 1183 1606 1741 174 1396 Fragments arranged by size: 1741 1606 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2243 2522 2720 2831 4744 4931 Size: 110 252 181 279 198 111 1913 187 Cuts at: 4931 1700 Size: 2869 Fragments arranged by size: 2869 1913 279 252 198 187 181 111 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 2105 2835 1925 Size: 180 730 5190 Fragments arranged by size: 5190 730 180 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 2338 2959 794 Size: 1544 621 3935 Fragments arranged by size: 3935 1544 621 BstBI TT'CG_AA Cuts at: 2658 4904 2658 Size: 2246 3854 BstEII G'GTnAC_C Cuts at: 1184 2827 1184 Size: 1643 4457 BstXI CCAn_nnnn'nTGG Cuts at: 2167 2269 2893 2167 Size: 102 624 5374 Fragments arranged by size: 5374 624 102 BstYI r'GATC_y Cuts at: 1972 3430 3797 4182 4199 5651 5737 5749 6095 Size: 1458 367 385 17 1452 86 12 346 Cuts at: 6095 1972 Size: 1977 Fragments arranged by size: 1977 1458 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2115 2125 2160 2268 2689 2785 Size: 552 957 93 10 35 108 421 96 Cuts at: 2785 513 Size: 3828 Fragments arranged by size: 3828 957 552 421 108 96 93 35 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 3616 4410 4430 3616 Size: 794 20 5286 Fragments arranged by size: 5286 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DraIII CAC_nnn'GTG Cuts at: 2050 2050 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2520 2673 Size: 54 1425 50 275 18 411 279 153 Cuts at: 2673 2683 2718 3031 3547 4436 6065 8 Size: 10 35 313 516 889 1629 43 Fragments arranged by size: 1629 1425 889 516 411 313 279 275 153 54 50 43 35 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2683 2718 3031 3547 1537 Size: 275 871 35 313 516 4090 Fragments arranged by size: 4090 871 516 313 275 35 EarI CTCTTCn'nnn_ Cuts at: 1353 2868 4030 1353 Size: 1515 1162 3423 Fragments arranged by size: 3423 1515 1162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2214 2274 4687 5061 5207 387 Size: 1064 138 625 60 2413 374 146 1280 Fragments arranged by size: 2413 1280 1064 625 374 146 138 60 EcoICRI GAG'CTC Cuts at: 2917 2917 Size: 6100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2536 3034 4163 4760 5547 5815 1239 Size: 393 904 498 1129 597 787 268 1524 Fragments arranged by size: 1524 1129 904 787 597 498 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3046 3046 Size: 6100 EcoO109I rG'GnC_Cy Cuts at: 2224 2979 2224 Size: 755 5345 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FseI GG_CCGG'CC Cuts at: 2247 2247 Size: 6100 FspI TGC'GCA Cuts at: 2999 4606 2999 Size: 1607 4493 HaeII r_GCGC'y Cuts at: 1678 2425 2926 5247 1678 Size: 747 501 2321 2531 Fragments arranged by size: 2531 2321 747 501 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4741 4773 4815 4847 1373 Size: 32 135 32 3169 32 42 32 2626 Fragments arranged by size: 3169 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3690 669 Size: 988 281 1506 246 3079 Fragments arranged by size: 3079 1506 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3255 5214 5398 1305 Size: 1950 1959 184 2007 Fragments arranged by size: 2007 1959 1950 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4278 Fragments arranged by size: 4278 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2267 2456 4058 4417 4576 518 Size: 648 999 102 189 1602 359 159 2042 Fragments arranged by size: 2042 1602 999 648 359 189 159 102 MspA1I CmG'CkG Cuts at: 1606 2017 2127 2193 2338 3268 3516 4193 5341 Size: 411 110 66 145 930 248 677 1148 Cuts at: 5341 5586 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 930 677 411 248 245 145 110 66 NaeI GCC'GGC Cuts at: 2245 2524 2722 2245 Size: 279 198 5623 Fragments arranged by size: 5623 279 198 NcoI C'CATG_G Cuts at: 513 1065 2022 2160 2268 513 Size: 552 957 138 108 4345 Fragments arranged by size: 4345 957 552 138 108 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NgoMIV G'CCGG_C Cuts at: 2243 2522 2720 2243 Size: 279 198 5623 Fragments arranged by size: 5623 279 198 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2302 3091 3114 3209 3458 5003 1132 Size: 97 1073 789 23 95 249 1545 2229 Fragments arranged by size: 2229 1545 1073 789 249 97 95 23 PasI CC'CwG_GG Cuts at: 1348 2779 2974 1348 Size: 1431 195 4474 Fragments arranged by size: 4474 1431 195 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 3012 3268 1108 Size: 1317 587 256 3940 Fragments arranged by size: 3940 1317 587 256 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3062 3094 4169 4201 5682 5714 3062 Size: 32 1075 32 1481 32 3448 Fragments arranged by size: 3448 1481 1075 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2979 2979 Size: 6100 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspOMI G'GGCC_C Cuts at: 2223 2223 Size: 6100 PstI C_TGCA'G Cuts at: 830 2334 3069 3452 830 Size: 1504 735 383 3478 Fragments arranged by size: 3478 1504 735 383 PvuI CG_AT'CG Cuts at: 2001 2034 2001 Size: 33 6067 PvuII CAG'CTG Cuts at: 2017 3268 2017 Size: 1251 4849 SacI G_AGCT'C Cuts at: 2919 2919 Size: 6100 SacII CC_GC'GG Cuts at: 2128 2128 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SanDI GG'GwC_CC Cuts at: 2979 2979 Size: 6100 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2330 2926 3065 3448 4583 5264 5455 Size: 219 1285 596 139 383 1135 681 191 Cuts at: 5455 826 Size: 1471 Fragments arranged by size: 1471 1285 1135 681 596 383 219 191 139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 2613 2810 3427 1872 Size: 741 197 617 4545 Fragments arranged by size: 4545 741 617 197 SmlI C'TyrA_G Cuts at: 820 1017 1943 2918 4204 5105 5367 820 Size: 197 926 975 1286 901 262 1553 Fragments arranged by size: 1553 1286 975 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 2302 3114 3458 1132 Size: 1170 812 344 3774 Fragments arranged by size: 3774 1170 812 344 SspI AAT'ATT Cuts at: 5 52 3213 4024 5 Size: 47 3161 811 2081 Fragments arranged by size: 3161 2081 811 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 2022 2160 2268 3539 513 Size: 552 957 138 108 1271 3074 Fragments arranged by size: 3074 1271 957 552 138 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3172 4127 4144 4297 4482 4927 325 Size: 1255 1592 955 17 153 185 445 1498 Fragments arranged by size: 1592 1498 1255 955 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4346 6058 96 Size: 274 80 33 51 1443 2369 1712 138 Fragments arranged by size: 2369 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2141 2173 6060 6092 2141 Size: 32 3887 32 2149 Fragments arranged by size: 3887 2149 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2560 2266 Size: 294 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 2611 2808 3425 1870 Size: 741 197 617 4545 Fragments arranged by size: 4545 741 617 197 XmnI GAAnn'nnTTC Cuts at: 2857 4229 2857 Size: 1372 4728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI