(Circular) (Six-base) MAPSORT of: pFN21AB9726.seq Check: 9160 from: 1 to: 5998 pFN21AB9726 5998 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4061 5445 5713 1632 Size: 2429 1384 268 1917 Fragments arranged by size: 2429 1917 1384 268 AfeI AGC'GCT Cuts at: 2150 2150 Size: 5998 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5998 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2606 2638 2606 Size: 32 5966 AlwNI CAG_nnn'CTG Cuts at: 2173 3741 5313 2173 Size: 1568 1572 2858 Fragments arranged by size: 2858 1572 1568 ApaI G_GGCC'C Cuts at: 2331 2780 2331 Size: 449 5549 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 3307 3533 3685 5794 1493 Size: 1814 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 2243 2551 2984 3323 1870 Size: 73 300 308 433 339 4545 Fragments arranged by size: 4545 433 339 308 300 73 BamHI G'GATC_C Cuts at: 3082 3328 3695 3082 Size: 246 367 5385 Fragments arranged by size: 5385 367 246 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 2951 2980 3020 3169 Size: 325 217 627 576 588 29 40 149 Cuts at: 3169 3181 3319 4774 618 Size: 12 138 1455 1842 Fragments arranged by size: 1842 1455 627 588 576 325 217 149 138 40 29 12 BanII G_rGCy'C Cuts at: 1391 2211 2250 2331 2549 2780 2870 3317 1391 Size: 820 39 81 218 231 90 447 4072 Fragments arranged by size: 4072 820 447 231 218 90 81 39 BbeI G_GCGC'C Cuts at: 2955 2984 3024 3173 3185 2955 Size: 29 40 149 12 5768 Fragments arranged by size: 5768 149 40 29 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BbvCI CC'TCA_GC Cuts at: 2684 2684 Size: 5998 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2065 2099 4189 4223 1903 Size: 34 50 34 44 34 2090 34 3678 Fragments arranged by size: 3678 2090 50 44 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4609 136 Size: 107 122 71 631 605 2937 1525 Fragments arranged by size: 2937 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2703 5993 2703 Size: 3290 2708 BlpI GC'TnA_GC Cuts at: 2152 3415 2152 Size: 1263 4735 Bme1580I G_kGCm'C Cuts at: 1790 2331 2780 4059 5215 1790 Size: 541 449 1279 1156 2573 Fragments arranged by size: 2573 1279 1156 541 449 BmgBI CAC'GTC Cuts at: 1318 3001 1318 Size: 1683 4315 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2274 3729 4687 Size: 416 53 83 399 24 857 1455 958 Cuts at: 4687 442 Size: 1753 Fragments arranged by size: 1753 1455 958 857 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2633 2769 4658 1239 Size: 914 480 136 1889 2579 Fragments arranged by size: 2579 1889 914 480 136 Bpu10I CC'TnA_GC Cuts at: 2684 2684 Size: 5998 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4123 4988 5286 4123 Size: 865 298 4835 Fragments arranged by size: 4835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3061 3789 493 Size: 711 1857 728 2702 Fragments arranged by size: 2702 1857 728 711 BsaBI GATnn'nnATC Cuts at: 2702 5992 2702 Size: 3290 2708 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2564 2952 2981 3021 Size: 53 83 186 936 1031 388 29 40 Cuts at: 3021 3032 3170 3182 3805 4187 4822 275 Size: 11 138 12 623 382 635 1451 Fragments arranged by size: 1451 1031 936 635 623 388 382 186 138 83 53 40 29 12 11 BsaWI w'CCGG_w Cuts at: 3052 4431 4829 5103 5250 5661 3052 Size: 1379 398 274 147 411 3389 Fragments arranged by size: 3389 1379 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2606 2636 1178 Size: 30 165 30 1203 30 4540 Fragments arranged by size: 4540 1203 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3226 3243 3246 1186 Size: 2040 17 3 3938 Fragments arranged by size: 3938 2040 17 3 BseYI C'CCAG_C Cuts at: 2330 2855 3185 5201 5697 2330 Size: 525 330 2016 496 2631 Fragments arranged by size: 2631 2016 525 496 330 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5425 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2823 3448 4209 5237 1540 Size: 275 186 822 625 761 1028 2301 Fragments arranged by size: 2301 1028 822 761 625 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 4059 4144 5215 2211 Size: 1848 85 1071 2994 Fragments arranged by size: 2994 1848 1071 85 BsmI GAATG_Cn' Cuts at: 2908 3509 3602 2908 Size: 601 93 5304 Fragments arranged by size: 5304 601 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2250 2331 2549 2780 2870 3317 Size: 399 421 39 81 218 231 90 447 Cuts at: 3317 4059 4144 5215 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 421 399 231 218 90 85 81 39 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 2829 3887 5617 2829 Size: 1058 1730 3210 Fragments arranged by size: 3210 1730 1058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2682 3353 844 Size: 1838 671 3489 Fragments arranged by size: 3489 1838 671 BsrBI CCG'CTC Cuts at: 1394 1823 2692 3885 1394 Size: 429 869 1193 3507 Fragments arranged by size: 3507 1193 869 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2083 4493 4667 65 Size: 1183 835 2410 174 1396 Fragments arranged by size: 2410 1396 1183 835 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2646 2718 2789 2822 3101 4642 4829 Size: 110 836 72 71 33 279 1541 187 Cuts at: 4829 1700 Size: 2869 Fragments arranged by size: 2869 1541 836 279 187 110 72 71 33 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5998 BssHII G'CGCG_C Cuts at: 1925 2595 1925 Size: 670 5328 BssSI C'ACGA_G Cuts at: 2590 4058 5070 2590 Size: 1468 1012 3518 Fragments arranged by size: 3518 1468 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2809 794 Size: 2015 3983 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2131 2758 2131 Size: 627 5371 BstYI r'GATC_y Cuts at: 1972 2703 3082 3328 3695 4080 4097 5549 5635 Size: 731 379 246 367 385 17 1452 86 Cuts at: 5635 5647 5993 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 731 385 379 367 346 246 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3258 3258 Size: 5998 BtgI C'CryG_G Cuts at: 513 1065 2034 2817 3196 513 Size: 552 969 783 379 3315 Fragments arranged by size: 3315 969 783 552 379 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5259 Fragments arranged by size: 5259 683 56 BtsI GCAGTG_nn' Cuts at: 2503 3514 4308 4328 2503 Size: 1011 794 20 4173 Fragments arranged by size: 4173 1011 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2667 2820 3445 Size: 54 1425 50 275 18 837 153 625 Cuts at: 3445 4334 5963 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 837 625 275 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2820 3445 1537 Size: 275 1008 625 4090 Fragments arranged by size: 4090 1008 625 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 3928 1353 Size: 698 1877 3423 Fragments arranged by size: 3423 1877 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2505 4585 4959 5105 387 Size: 1064 138 916 2080 374 146 1280 Fragments arranged by size: 2080 1280 1064 916 374 146 138 EcoICRI GAG'CTC Cuts at: 2209 2209 Size: 5998 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2633 2769 4061 4658 5445 5713 Size: 393 521 480 136 1292 597 787 268 Cuts at: 5713 1239 Size: 1524 Fragments arranged by size: 1524 1292 787 597 521 480 393 268 136 EcoNI CCTnn'n_nnAGG Cuts at: 2940 2940 Size: 5998 EcoO109I rG'GnC_Cy Cuts at: 2776 2776 Size: 5998 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 2152 2955 2984 3024 3173 3185 3281 5145 Size: 474 803 29 40 149 12 96 1864 Cuts at: 5145 1678 Size: 2531 Fragments arranged by size: 2531 1864 803 474 149 96 40 29 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2218 2237 2250 2269 2855 Size: 32 135 32 646 19 13 19 586 Cuts at: 2855 2887 3221 3253 4639 4671 4713 4745 1373 Size: 32 334 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1386 646 586 334 135 42 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KasI G'GCGC_C Cuts at: 2951 2980 3020 3169 3181 2951 Size: 29 40 149 12 5768 Fragments arranged by size: 5768 149 40 29 12 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2946 2996 5112 5296 1305 Size: 1641 50 2116 184 2007 Fragments arranged by size: 2116 2007 1641 184 50 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 10 Size: 54 1425 343 837 3339 Fragments arranged by size: 3339 1425 837 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2901 3956 4315 4474 518 Size: 648 1735 1055 359 159 2042 Fragments arranged by size: 2042 1735 1055 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2036 2220 2508 2602 3088 3414 4091 5239 Size: 430 184 288 94 486 326 677 1148 Cuts at: 5239 5484 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 486 430 326 288 245 184 94 NaeI GCC'GGC Cuts at: 2648 2791 2824 3103 2648 Size: 143 33 279 5543 Fragments arranged by size: 5543 279 143 33 NarI GG'CG_CC Cuts at: 2952 2981 3021 3170 3182 2952 Size: 29 40 149 12 5768 Fragments arranged by size: 5768 149 40 29 12 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5446 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NgoMIV G'CCGG_C Cuts at: 2646 2789 2822 3101 2646 Size: 143 33 279 5543 Fragments arranged by size: 5543 279 143 33 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 2195 3356 4901 1132 Size: 97 966 1161 1545 2229 Fragments arranged by size: 2229 1545 1161 966 97 PasI CC'CwG_GG Cuts at: 1348 2173 1348 Size: 825 5173 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PfoI T'CCnGG_A Cuts at: 3146 3146 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2606 2638 4067 4099 5580 5612 2606 Size: 32 1429 32 1481 32 2992 Fragments arranged by size: 2992 1481 1429 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PspOMI G'GGCC_C Cuts at: 2327 2776 2327 Size: 449 5549 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3143 3175 3143 Size: 32 5966 PstI C_TGCA'G Cuts at: 830 2169 3350 830 Size: 1339 1181 3478 Fragments arranged by size: 3478 1339 1181 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2220 2602 2220 Size: 382 5616 SacI G_AGCT'C Cuts at: 2211 2211 Size: 5998 SacII CC_GC'GG Cuts at: 2037 2037 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SexAI A'CCwGG_T Cuts at: 3294 3294 Size: 5998 SfcI C'TryA_G Cuts at: 826 1045 2165 3346 4481 5162 5353 826 Size: 219 1120 1181 1135 681 191 1471 Fragments arranged by size: 1471 1181 1135 1120 681 219 191 SfoI GGC'GCC Cuts at: 2953 2982 3022 3171 3183 2953 Size: 29 40 149 12 5768 Fragments arranged by size: 5768 149 40 29 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 2553 3325 1872 Size: 681 772 4545 Fragments arranged by size: 4545 772 681 SmlI C'TyrA_G Cuts at: 820 1017 1943 4102 5003 5265 820 Size: 197 926 2159 901 262 1553 Fragments arranged by size: 2159 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2751 3437 513 Size: 552 1686 686 3074 Fragments arranged by size: 3074 1686 686 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4025 4042 4195 4380 4825 325 Size: 1255 2445 17 153 185 445 1498 Fragments arranged by size: 2445 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 4244 5956 Size: 274 80 33 51 1443 753 1514 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 1712 1514 1443 753 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5958 5990 5958 Size: 32 5966 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 2551 3323 1870 Size: 681 772 4545 Fragments arranged by size: 4545 772 681 XmnI GAAnn'nnTTC Cuts at: 2621 4127 2621 Size: 1506 4492 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI RsrII SanDI SapI SfiI SrfI SwaI XcmI