Restriction map for
pFN21AB9726
(Linear) (Six-base) MAPSORT of: KIBB9726.seq Check: 8117 from: 1 to: 1293
KIBB9726.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AfeI AGC'GCT
Cuts at: 0 145 1293
Size: 145 1148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 601 633 1293
Size: 601 32 660
Fragments arranged by size:
660 601 32
AlwNI CAG_nnn'CTG
Cuts at: 0 168 1293
Size: 168 1125
ApaI G_GGCC'C
Cuts at: 0 326 775 1293
Size: 326 449 518
Fragments arranged by size:
518 449 326
AvaI C'yCGr_G
Cuts at: 0 238 546 979 1293
Size: 238 308 433 314
Fragments arranged by size:
433 314 308 238
BamHI G'GATC_C
Cuts at: 0 1077 1293
Size: 1077 216
BanI G'GyrC_C
Cuts at: 0 358 946 975 1015 1164 1176 1293
Size: 358 588 29 40 149 12 117
Fragments arranged by size:
588 358 149 117 40 29 12
BanII G_rGCy'C
Cuts at: 0 206 245 326 544 775 865 1293
Size: 206 39 81 218 231 90 428
Fragments arranged by size:
428 231 218 206 90 81 39
BbeI G_GCGC'C
Cuts at: 0 950 979 1019 1168 1180 1293
Size: 950 29 40 149 12 113
Fragments arranged by size:
950 149 113 40 29 12
BbvCI CC'TCA_GC
Cuts at: 0 679 1293
Size: 679 614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 60 94 1293
Size: 60 34 1199
Fragments arranged by size:
1199 60 34
BglII A'GATC_T
Cuts at: 0 698 1293
Size: 698 595
BlpI GC'TnA_GC
Cuts at: 0 147 1293
Size: 147 1146
Bme1580I G_kGCm'C
Cuts at: 0 326 775 1293
Size: 326 449 518
Fragments arranged by size:
518 449 326
BmgBI CAC'GTC
Cuts at: 0 996 1293
Size: 996 297
BmrI ACTGGGnnnn_n'
Cuts at: 0 269 1293
Size: 269 1024
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 628 764 1293
Size: 148 480 136 529
Fragments arranged by size:
529 480 148 136
Bpu10I CC'TnA_GC
Cuts at: 0 679 1293
Size: 679 614
BsaAI yAC'GTr
Cuts at: 0 1056 1293
Size: 1056 237
BsaBI GATnn'nnATC
Cuts at: 0 697 1293
Size: 697 596
BsaHI Gr'CG_yC
Cuts at: 0 559 947 976 1016 1027 1165 1177 1293
Size: 559 388 29 40 11 138 12 116
Fragments arranged by size:
559 388 138 116 40 29 12 11
BsaWI w'CCGG_w
Cuts at: 0 1047 1293
Size: 1047 246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 601 631 1293
Size: 601 30 662
Fragments arranged by size:
662 601 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1221 1238 1241 1293
Size: 1221 17 3 52
Fragments arranged by size:
1221 52 17 3
BseYI C'CCAG_C
Cuts at: 0 325 850 1180 1293
Size: 325 525 330 113
Fragments arranged by size:
525 330 325 113
BsiEI CG_ry'CG
Cuts at: 0 818 1293
Size: 818 475
BsiHKAI G_wGCw'C
Cuts at: 0 206 1293
Size: 206 1087
BsmI GAATG_Cn'
Cuts at: 0 903 1293
Size: 903 390
Bsp1286I G_dGCh'C
Cuts at: 0 206 245 326 544 775 865 1293
Size: 206 39 81 218 231 90 428
Fragments arranged by size:
428 231 218 206 90 81 39
BspHI T'CATG_A
Cuts at: 0 824 1293
Size: 824 469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 677 1293
Size: 677 616
BsrBI CCG'CTC
Cuts at: 0 687 1293
Size: 687 606
BsrDI GCAATG_nn'
Cuts at: 0 78 1293
Size: 78 1215
BsrFI r'CCGG_y
Cuts at: 0 641 713 784 817 1096 1293
Size: 641 72 71 33 279 197
Fragments arranged by size:
641 279 197 72 71 33
BssHII G'CGCG_C
Cuts at: 0 590 1293
Size: 590 703
BssSI C'ACGA_G
Cuts at: 0 585 1293
Size: 585 708
BstAPI GCAn_nnn'nTGC
Cuts at: 0 804 1293
Size: 804 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 753 1293
Size: 126 627 540
Fragments arranged by size:
627 540 126
BstYI r'GATC_y
Cuts at: 0 698 1077 1293
Size: 698 379 216
Fragments arranged by size:
698 379 216
Bsu36I CC'TnA_GG
Cuts at: 0 1253 1293
Size: 1253 40
BtgI C'CryG_G
Cuts at: 0 29 812 1191 1293
Size: 29 783 379 102
Fragments arranged by size:
783 379 102 29
BtsI GCAGTG_nn'
Cuts at: 0 498 1293
Size: 498 795
EaeI y'GGCC_r
Cuts at: 0 662 815 1293
Size: 662 153 478
Fragments arranged by size:
662 478 153
EagI C'GGCC_G
Cuts at: 0 815 1293
Size: 815 478
EarI CTCTTCn'nnn_
Cuts at: 0 46 1293
Size: 46 1247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 500 1293
Size: 500 793
EcoICRI GAG'CTC
Cuts at: 0 204 1293
Size: 204 1089
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 628 764 1293
Size: 148 480 136 529
Fragments arranged by size:
529 480 148 136
EcoNI CCTnn'n_nnAGG
Cuts at: 0 935 1293
Size: 935 358
EcoO109I rG'GnC_Cy
Cuts at: 0 771 1293
Size: 771 522
HaeII r_GCGC'y
Cuts at: 0 147 950 979 1019 1168 1180 1276 1293
Size: 147 803 29 40 149 12 96 17
Fragments arranged by size:
803 149 147 96 40 29 17 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 213 232 245 264 850 882 1216 1248
Size: 213 19 13 19 586 32 334 32
Cuts at: 1248 1293
Size: 45
Fragments arranged by size:
586 334 213 45 32 32 19 19
13
KasI G'GCGC_C
Cuts at: 0 946 975 1015 1164 1176 1293
Size: 946 29 40 149 12 117
Fragments arranged by size:
946 149 117 40 29 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 991 1293
Size: 941 50 302
Fragments arranged by size:
941 302 50
MscI TGG'CCA
Cuts at: 0 664 1293
Size: 664 629
MslI CAynn'nnrTG
Cuts at: 0 896 1293
Size: 896 397
MspA1I CmG'CkG
Cuts at: 0 31 215 503 597 1083 1293
Size: 31 184 288 94 486 210
Fragments arranged by size:
486 288 210 184 94 31
NaeI GCC'GGC
Cuts at: 0 643 786 819 1098 1293
Size: 643 143 33 279 195
Fragments arranged by size:
643 279 195 143 33
NarI GG'CG_CC
Cuts at: 0 947 976 1016 1165 1177 1293
Size: 947 29 40 149 12 116
Fragments arranged by size:
947 149 116 40 29 12
NgoMIV G'CCGG_C
Cuts at: 0 641 784 817 1096 1293
Size: 641 143 33 279 197
Fragments arranged by size:
641 279 197 143 33
NspI r_CATG'y
Cuts at: 0 190 1293
Size: 190 1103
PasI CC'CwG_GG
Cuts at: 0 168 1293
Size: 168 1125
PfoI T'CCnGG_A
Cuts at: 0 1141 1293
Size: 1141 152
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 1293
Size: 601 32 660
Fragments arranged by size:
660 601 32
PspOMI G'GGCC_C
Cuts at: 0 322 771 1293
Size: 322 449 522
Fragments arranged by size:
522 449 322
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1293
Size: 1138 32 123
Fragments arranged by size:
1138 123 32
PstI C_TGCA'G
Cuts at: 0 164 1293
Size: 164 1129
PvuII CAG'CTG
Cuts at: 0 215 597 1293
Size: 215 382 696
Fragments arranged by size:
696 382 215
SacI G_AGCT'C
Cuts at: 0 206 1293
Size: 206 1087
SacII CC_GC'GG
Cuts at: 0 32 1293
Size: 32 1261
SfcI C'TryA_G
Cuts at: 0 160 1293
Size: 160 1133
SfoI GGC'GCC
Cuts at: 0 948 977 1017 1166 1178 1293
Size: 948 29 40 149 12 115
Fragments arranged by size:
948 149 115 40 29 12
SmaI CCC'GGG
Cuts at: 0 548 1293
Size: 548 745
StyI C'CwwG_G
Cuts at: 0 746 1293
Size: 746 547
TatI w'GTAC_w
Cuts at: 0 725 1293
Size: 725 568
XmaI C'CCGG_G
Cuts at: 0 546 1293
Size: 546 747
XmnI GAAnn'nnTTC
Cuts at: 0 616 1293
Size: 616 677
Enzymes that do cut:
AfeI AloI AlwNI ApaI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PfoI PpiI
PspOMI PsrI PstI PvuII SacI SacII SfcI SfoI
SmaI StyI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BciVI BclI BfrBI BglI
BmtI BplI BpuEI BsaI BsgI BsiWI BsmBI BspEI
BsrGI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpuMI PshAI PsiI PspXI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI ZraI