(Circular) (Six-base) MAPSORT of: pFN21AB9723.seq Check: 8196 from: 1 to: 5947 pFN21AB9723 5947 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2425 2425 Size: 5947 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3290 1937 Size: 1353 4594 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2468 4010 5394 5662 1632 Size: 836 1542 1384 268 1917 Fragments arranged by size: 1917 1542 1384 836 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 2673 2886 4846 5823 1203 Size: 1470 213 1960 977 1327 Fragments arranged by size: 1960 1470 1327 977 213 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3043 1184 Size: 1859 4088 AlwNI CAG_nnn'CTG Cuts at: 2629 3103 3690 5262 2629 Size: 474 587 1572 3314 Fragments arranged by size: 3314 1572 587 474 ApaI G_GGCC'C Cuts at: 2090 2510 2993 2090 Size: 420 483 5044 Fragments arranged by size: 5044 483 420 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 3089 3256 3482 3634 5743 1493 Size: 1596 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 1596 226 167 152 AscI GG'CGCG_CC Cuts at: 2634 2634 Size: 5947 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 2510 2555 2729 2912 2986 3068 3272 Size: 73 567 45 174 183 74 82 204 Cuts at: 3272 1870 Size: 4545 Fragments arranged by size: 4545 567 204 183 174 82 74 73 45 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2017 2050 2017 Size: 33 5914 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2750 2866 3268 4723 Size: 325 217 627 359 604 116 402 1455 Cuts at: 4723 618 Size: 1842 Fragments arranged by size: 1842 1455 627 604 402 359 325 217 116 BanII G_rGCy'C Cuts at: 1391 2090 2117 2510 2838 2909 2993 3066 3266 Size: 699 27 393 328 71 84 73 200 Cuts at: 3266 1391 Size: 4072 Fragments arranged by size: 4072 699 393 328 200 84 73 71 27 BbeI G_GCGC'C Cuts at: 2150 2870 2150 Size: 720 5227 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4976 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4138 4172 1903 Size: 34 2201 34 3678 Fragments arranged by size: 3678 2201 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2018 2216 3839 5049 2018 Size: 198 1623 1210 2916 Fragments arranged by size: 2916 1623 1210 198 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2425 2514 3036 Size: 107 122 71 631 605 753 89 522 Cuts at: 3036 4558 136 Size: 1522 1525 Fragments arranged by size: 1525 1522 753 631 605 522 122 107 89 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 2111 2484 3364 2111 Size: 373 880 4694 Fragments arranged by size: 4694 880 373 Bme1580I G_kGCm'C Cuts at: 1790 2090 2510 2993 4008 5164 1790 Size: 300 420 483 1015 1156 2573 Fragments arranged by size: 2573 1156 1015 483 420 300 BmgBI CAC'GTC Cuts at: 1318 2676 3244 1318 Size: 1358 568 4021 Fragments arranged by size: 4021 1358 568 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3042 3678 4636 Size: 416 53 83 399 24 1625 636 958 Cuts at: 4636 442 Size: 1753 Fragments arranged by size: 1753 1625 958 636 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 4607 1239 Size: 1292 2076 2579 Fragments arranged by size: 2579 2076 1292 Bpu10I CC'TnA_GC Cuts at: 2241 2241 Size: 5947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4072 4937 5235 4072 Size: 865 298 4784 Fragments arranged by size: 4784 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2776 4610 882 Size: 617 1277 1834 2219 Fragments arranged by size: 2219 1834 1277 617 BsaAI yAC'GTr Cuts at: 493 1204 2887 3738 493 Size: 711 1683 851 2702 Fragments arranged by size: 2702 1683 851 711 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 2867 3207 3754 Size: 53 83 186 936 614 720 340 547 Cuts at: 3754 4136 4771 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 720 635 614 547 382 340 186 83 53 BsaWI w'CCGG_w Cuts at: 3013 3155 4380 4778 5052 5199 5610 3013 Size: 142 1225 398 274 147 411 3350 Fragments arranged by size: 3350 1225 411 398 274 147 142 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3197 3227 1178 Size: 30 165 30 1794 30 3898 Fragments arranged by size: 3898 1794 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 3056 1186 Size: 1189 681 4077 Fragments arranged by size: 4077 1189 681 BseYI C'CCAG_C Cuts at: 2083 2308 2503 2873 5150 5646 2083 Size: 225 195 370 2277 496 2384 Fragments arranged by size: 2384 2277 496 370 225 195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2267 2402 2456 3089 1163 Size: 573 531 135 54 633 4021 Fragments arranged by size: 4021 633 573 531 135 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2172 2216 2420 2499 2521 2748 Size: 275 186 171 44 204 79 22 227 Cuts at: 2748 3397 4158 5186 1540 Size: 649 761 1028 2301 Fragments arranged by size: 2301 1028 761 649 275 227 204 186 171 79 44 22 BsiHKAI G_wGCw'C Cuts at: 2167 3066 4008 4093 5164 2167 Size: 899 942 85 1071 2950 Fragments arranged by size: 2950 1071 942 899 85 BsmI GAATG_Cn' Cuts at: 3458 3551 3458 Size: 93 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2117 2167 2510 2838 2909 2993 Size: 399 300 27 50 343 328 71 84 Cuts at: 2993 3066 3266 4008 4093 5164 1391 Size: 73 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 328 300 200 85 84 73 71 50 27 BspEI T'CCGG_A Cuts at: 3013 5610 3013 Size: 2597 3350 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2289 2425 2758 3302 844 Size: 1445 136 333 544 3489 Fragments arranged by size: 3489 1445 544 333 136 BsrBI CCG'CTC Cuts at: 1394 1823 2371 2644 3834 1394 Size: 429 548 273 1190 3507 Fragments arranged by size: 3507 1190 548 429 273 BsrDI GCAATG_nn' Cuts at: 65 1248 4442 4616 65 Size: 1183 3194 174 1396 Fragments arranged by size: 3194 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2149 2167 2415 2573 2747 2804 4591 Size: 110 339 18 248 158 174 57 1787 Cuts at: 4591 4778 1700 Size: 187 2869 Fragments arranged by size: 2869 1787 339 248 187 174 158 110 57 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 2058 2634 1925 Size: 133 576 5238 Fragments arranged by size: 5238 576 133 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 2389 794 Size: 1595 4352 BstBI TT'CG_AA Cuts at: 4751 4751 Size: 5947 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstYI r'GATC_y Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942 Size: 1305 367 385 17 1452 86 12 346 Cuts at: 5942 1972 Size: 1977 Fragments arranged by size: 1977 1452 1305 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2067 2178 2420 2529 2838 3178 3216 Size: 552 1002 111 242 109 309 340 38 Cuts at: 3216 513 Size: 3244 Fragments arranged by size: 3244 1002 552 340 309 242 111 109 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2915 487 Size: 683 56 1689 3519 Fragments arranged by size: 3519 1689 683 56 BtsI GCAGTG_nn' Cuts at: 3020 3463 4257 4277 3020 Size: 443 794 20 4690 Fragments arranged by size: 4690 794 443 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 3251 3251 Size: 5947 DraIII CAC_nnn'GTG Cuts at: 3112 3112 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2169 2213 Size: 54 1425 50 275 18 246 93 44 Cuts at: 2213 2417 2496 2518 2526 2605 2745 3394 4283 Size: 204 79 22 8 79 140 649 889 Cuts at: 4283 5912 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 649 275 246 204 140 93 79 79 54 50 44 43 22 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2169 2213 2417 2496 2518 2745 3394 Size: 275 357 44 204 79 22 227 649 Cuts at: 3394 1537 Size: 4090 Fragments arranged by size: 4090 649 357 275 227 204 79 44 22 EarI CTCTTCn'nnn_ Cuts at: 1353 3877 1353 Size: 2524 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2354 4534 4908 5054 387 Size: 1064 138 765 2180 374 146 1280 Fragments arranged by size: 2180 1280 1064 765 374 146 138 EcoICRI GAG'CTC Cuts at: 3064 3064 Size: 5947 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 2531 4010 4607 5394 5662 1239 Size: 393 836 63 1479 597 787 268 1524 Fragments arranged by size: 1524 1479 836 787 597 393 268 63 EcoNI CCTnn'n_nnAGG Cuts at: 3071 3071 Size: 5947 EcoO109I rG'GnC_Cy Cuts at: 2087 2507 2843 2990 2087 Size: 420 336 147 5044 Fragments arranged by size: 5044 420 336 147 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 5947 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 2150 2330 2870 2880 5094 1678 Size: 472 180 540 10 2214 2531 Fragments arranged by size: 2531 2214 540 472 180 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3197 3229 4588 4620 4662 Size: 32 135 32 1625 32 1359 32 42 Cuts at: 4662 4694 1373 Size: 32 2626 Fragments arranged by size: 2626 1625 1359 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3291 3537 669 Size: 988 281 1353 246 3079 Fragments arranged by size: 3079 1353 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5947 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KasI G'GCGC_C Cuts at: 2146 2866 2146 Size: 720 5227 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3228 5061 5245 1305 Size: 1923 1833 184 2007 Fragments arranged by size: 2007 1923 1833 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 10 Size: 54 1425 343 696 3429 Fragments arranged by size: 3429 1425 696 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 3905 4264 4423 518 Size: 648 966 1773 359 159 2042 Fragments arranged by size: 2042 1773 966 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 2160 2180 2422 2584 2683 2840 3218 Size: 463 91 20 242 162 99 157 378 Cuts at: 3218 3363 4040 5188 5433 1606 Size: 145 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 463 378 245 242 162 157 145 99 91 20 NaeI GCC'GGC Cuts at: 2151 2575 2749 2151 Size: 424 174 5349 Fragments arranged by size: 5349 424 174 NarI GG'CG_CC Cuts at: 2147 2867 2147 Size: 720 5227 NcoI C'CATG_G Cuts at: 513 1065 2529 513 Size: 552 1464 3931 Fragments arranged by size: 3931 1464 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NgoMIV G'CCGG_C Cuts at: 2149 2573 2747 2149 Size: 424 174 5349 Fragments arranged by size: 5349 424 174 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 2496 2518 3394 2496 Size: 22 876 5049 Fragments arranged by size: 5049 876 22 NspI r_CATG'y Cuts at: 1132 1229 2392 3305 4850 1132 Size: 97 1163 913 1545 2229 Fragments arranged by size: 2229 1545 1163 913 97 PasI CC'CwG_GG Cuts at: 1348 2097 1348 Size: 749 5198 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 2629 1108 Size: 1521 4426 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PmlI CAC'GTG Cuts at: 2887 2887 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2843 2843 Size: 5947 PshAI GACnn'nnGTC Cuts at: 1613 3001 3048 1613 Size: 1388 47 4512 Fragments arranged by size: 4512 1388 47 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PspOMI G'GGCC_C Cuts at: 2086 2506 2989 2086 Size: 420 483 5044 Fragments arranged by size: 5044 483 420 PstI C_TGCA'G Cuts at: 830 3299 830 Size: 2469 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 SacI G_AGCT'C Cuts at: 3066 3066 Size: 5947 SacII CC_GC'GG Cuts at: 2070 2181 2423 2841 3219 2070 Size: 111 242 418 378 4798 Fragments arranged by size: 4798 418 378 242 111 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SanDI GG'GwC_CC Cuts at: 2843 2843 Size: 5947 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 2755 3295 4430 5111 5302 826 Size: 219 1710 540 1135 681 191 1471 Fragments arranged by size: 1710 1471 1135 681 540 219 191 SfoI GGC'GCC Cuts at: 2148 2868 2148 Size: 720 5227 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 2149 1700 Size: 449 5498 SmaI CCC'GGG Cuts at: 1872 2731 2914 2988 3274 1872 Size: 859 183 74 286 4545 Fragments arranged by size: 4545 859 286 183 74 SmlI C'TyrA_G Cuts at: 820 1017 1943 4051 4952 5214 820 Size: 197 926 2108 901 262 1553 Fragments arranged by size: 2108 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5947 StyI C'CwwG_G Cuts at: 513 1065 2190 2529 3386 513 Size: 552 1125 339 857 3074 Fragments arranged by size: 3074 1125 857 552 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2887 3974 3991 4144 4329 4774 325 Size: 1255 1307 1087 17 153 185 445 1498 Fragments arranged by size: 1498 1307 1255 1087 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2889 4193 5905 Size: 274 80 33 51 1443 912 1304 1712 Cuts at: 5905 96 Size: 138 Fragments arranged by size: 1712 1443 1304 912 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2536 3193 2536 Size: 657 5290 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 2729 2912 2986 3272 1870 Size: 859 183 74 286 4545 Fragments arranged by size: 4545 859 286 183 74 XmnI GAAnn'nnTTC Cuts at: 2225 4076 2225 Size: 1851 4096 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SapI SexAI SfiI SrfI SwaI