Restriction map for
pFN21AB9716
(Linear) (Six-base) MAPSORT of: KIBB9716.seq Check: 9105 from: 1 to: 1179
KIBB9716.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AatII G_ACGT'C
Cuts at: 0 755 1179
Size: 755 424
AflIII A'CryG_T
Cuts at: 0 881 1179
Size: 881 298
AleI CACnn'nnGTG
Cuts at: 0 50 137 185 1179
Size: 50 87 48 994
Fragments arranged by size:
994 87 50 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 955 1179
Size: 955 224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 784 816 1179
Size: 784 32 363
Fragments arranged by size:
784 363 32
AlwNI CAG_nnn'CTG
Cuts at: 0 708 1179
Size: 708 471
AvaI C'yCGr_G
Cuts at: 0 64 1133 1179
Size: 64 1069 46
Fragments arranged by size:
1069 64 46
BamHI G'GATC_C
Cuts at: 0 544 563 1179
Size: 544 19 616
Fragments arranged by size:
616 544 19
BanI G'GyrC_C
Cuts at: 0 1011 1179
Size: 1011 168
BanII G_rGCy'C
Cuts at: 0 628 1141 1179
Size: 628 513 38
Fragments arranged by size:
628 513 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 747 1179
Size: 747 432
Bme1580I G_kGCm'C
Cuts at: 0 386 965 1179
Size: 386 579 214
Fragments arranged by size:
579 386 214
BmgBI CAC'GTC
Cuts at: 0 102 1179
Size: 102 1077
BmrI ACTGGGnnnn_n'
Cuts at: 0 532 1047 1179
Size: 532 515 132
Fragments arranged by size:
532 515 132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 364 953 1179
Size: 364 589 226
Fragments arranged by size:
589 364 226
Bpu10I CC'TnA_GC
Cuts at: 0 866 1179
Size: 866 313
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 335 1179
Size: 211 124 844
Fragments arranged by size:
844 211 124
BsaAI yAC'GTr
Cuts at: 0 960 1179
Size: 960 219
BsaHI Gr'CG_yC
Cuts at: 0 271 752 1179
Size: 271 481 427
Fragments arranged by size:
481 427 271
BsaWI w'CCGG_w
Cuts at: 0 413 1179
Size: 413 766
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 784 814 976 1006 1179
Size: 13 30 741 30 162 30 173
Fragments arranged by size:
741 173 162 30 30 30 13
BseYI C'CCAG_C
Cuts at: 0 385 457 496 698 807 870 1179
Size: 385 72 39 202 109 63 309
Fragments arranged by size:
385 309 202 109 72 63 39
BsiEI CG_ry'CG
Cuts at: 0 87 1179
Size: 87 1092
BsiHKAI G_wGCw'C
Cuts at: 0 773 934 1179
Size: 773 161 245
Fragments arranged by size:
773 245 161
BsmBI CGTCTCn'nnnn_
Cuts at: 0 396 1179
Size: 396 783
Bsp1286I G_dGCh'C
Cuts at: 0 386 628 773 934 965 1141 1179
Size: 386 242 145 161 31 176 38
Fragments arranged by size:
386 242 176 161 145 38 31
BspEI T'CCGG_A
Cuts at: 0 413 1179
Size: 413 766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 197 1179
Size: 197 982
BsrFI r'CCGG_y
Cuts at: 0 41 86 220 344 1179
Size: 41 45 134 124 835
Fragments arranged by size:
835 134 124 45 41
BstEII G'GTnAC_C
Cuts at: 0 946 1179
Size: 946 233
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 1179
Size: 282 897
BstYI r'GATC_y
Cuts at: 0 544 563 1179
Size: 544 19 616
Fragments arranged by size:
616 544 19
BtgI C'CryG_G
Cuts at: 0 180 1179
Size: 180 999
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 49 1179
Size: 49 1130
BtsI GCAGTG_nn'
Cuts at: 0 9 1179
Size: 9 1170
EaeI y'GGCC_r
Cuts at: 0 84 353 480 972 1179
Size: 84 269 127 492 207
Fragments arranged by size:
492 269 207 127 84
EagI C'GGCC_G
Cuts at: 0 84 1179
Size: 84 1095
EarI CTCTTCn'nnn_
Cuts at: 0 399 589 1179
Size: 399 190 590
Fragments arranged by size:
590 399 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1131 1179
Size: 1131 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 1179
Size: 355 824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1127 1179
Size: 1127 52
FseI GG_CCGG'CC
Cuts at: 0 90 1179
Size: 90 1089
HaeII r_GCGC'y
Cuts at: 0 527 1179
Size: 527 652
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 1179
Size: 13 32 1134
Fragments arranged by size:
1134 32 13
HindIII A'AGCT_T
Cuts at: 0 713 1179
Size: 713 466
MscI TGG'CCA
Cuts at: 0 482 974 1179
Size: 482 492 205
Fragments arranged by size:
492 482 205
MslI CAynn'nnrTG
Cuts at: 0 50 137 185 1179
Size: 50 87 48 994
Fragments arranged by size:
994 87 50 48
MspA1I CmG'CkG
Cuts at: 0 168 708 1153 1162 1179
Size: 168 540 445 9 17
Fragments arranged by size:
540 445 168 17 9
NaeI GCC'GGC
Cuts at: 0 88 1179
Size: 88 1091
NgoMIV G'CCGG_C
Cuts at: 0 86 1179
Size: 86 1093
PasI CC'CwG_GG
Cuts at: 0 820 1179
Size: 820 359
PmlI CAC'GTG
Cuts at: 0 960 1179
Size: 960 219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 1179
Size: 784 32 363
Fragments arranged by size:
784 363 32
PvuII CAG'CTG
Cuts at: 0 168 708 1179
Size: 168 540 471
Fragments arranged by size:
540 471 168
ScaI AGT'ACT
Cuts at: 0 799 1179
Size: 799 380
SmaI CCC'GGG
Cuts at: 0 1135 1179
Size: 1135 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 455 712 1179
Size: 455 257 467
Fragments arranged by size:
467 455 257
TatI w'GTAC_w
Cuts at: 0 797 1055 1179
Size: 797 258 124
Fragments arranged by size:
797 258 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 509 1179
Size: 477 32 670
Fragments arranged by size:
670 477 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 537 867 996 1179
Size: 261 276 330 129 183
Fragments arranged by size:
330 276 261 183 129
XmaI C'CCGG_G
Cuts at: 0 1133 1179
Size: 1133 46
ZraI GAC'GTC
Cuts at: 0 753 1179
Size: 753 426
Enzymes that do cut:
AatII AflIII AleI AlfI AloI AlwNI AvaI BamHI
BanI BanII BbsI Bme1580I BmgBI BmrI BplI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrFI BstEII BstXI BstYI
BtgI BtgZI BtsI EaeI EagI EarI EciI EcoNI
FalI FseI HaeII Hin4I HindIII MscI MslI MspA1I
NaeI NgoMIV PasI PmlI PpiI PvuII ScaI SmaI
TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmtI BpmI BpuEI BsaBI BseRI BsgI
BsiWI BsmI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI Tth111I XbaI XhoI XmnI