(Circular) (Six-base) MAPSORT of: pFN21AB9710.seq Check: 5058 from: 1 to: 5749 pFN21AB9710 5749 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2863 2863 Size: 5749 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2124 2732 3092 1937 Size: 187 608 360 4594 Fragments arranged by size: 4594 608 360 187 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2053 3812 5196 5464 1632 Size: 421 1759 1384 268 1917 Fragments arranged by size: 1917 1759 1384 421 268 AfeI AGC'GCT Cuts at: 2223 2834 2223 Size: 611 5138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2398 2782 3492 5064 2398 Size: 384 710 1572 3083 Fragments arranged by size: 3083 1572 710 384 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2674 3074 1870 Size: 73 731 400 4545 Fragments arranged by size: 4545 731 400 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2711 3070 4525 618 Size: 325 217 627 924 359 1455 1842 Fragments arranged by size: 1842 1455 924 627 359 325 217 BanII G_rGCy'C Cuts at: 1391 2483 2955 3068 1391 Size: 1092 472 113 4072 Fragments arranged by size: 4072 1092 472 113 BbeI G_GCGC'C Cuts at: 2715 2715 Size: 5749 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2577 2676 928 Size: 971 678 99 4001 Fragments arranged by size: 4001 971 678 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2155 4360 136 Size: 107 122 71 631 605 483 2205 1525 Fragments arranged by size: 2205 1525 631 605 483 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 3810 4966 1790 Size: 2020 1156 2573 Fragments arranged by size: 2573 2020 1156 BmgBI CAC'GTC Cuts at: 1318 2730 2995 1318 Size: 1412 265 4072 Fragments arranged by size: 4072 1412 265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3480 4438 442 Size: 416 53 83 399 24 2063 958 1753 Fragments arranged by size: 2063 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2617 2617 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2263 2627 2708 2804 4409 1239 Size: 1024 364 81 96 1605 2579 Fragments arranged by size: 2579 1605 1024 364 96 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2687 4412 882 Size: 617 1188 1725 2219 Fragments arranged by size: 2219 1725 1188 617 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 2637 5743 2637 Size: 3106 2643 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2712 3556 3938 4573 Size: 53 83 186 936 1179 844 382 635 Cuts at: 4573 275 Size: 1451 Fragments arranged by size: 1451 1179 936 844 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5524 Fragments arranged by size: 5524 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2606 2705 1186 Size: 1420 99 4230 Fragments arranged by size: 4230 1420 99 BseYI C'CCAG_C Cuts at: 2050 2105 2255 2352 4952 5448 2050 Size: 55 150 97 2600 496 2351 Fragments arranged by size: 2600 2351 496 150 97 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2534 2891 1163 Size: 573 798 357 4021 Fragments arranged by size: 4021 798 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2159 2505 3199 3960 4988 1540 Size: 275 186 158 346 694 761 1028 2301 Fragments arranged by size: 2301 1028 761 694 346 275 186 158 BsiHKAI G_wGCw'C Cuts at: 3810 3895 4966 3810 Size: 85 1071 4593 Fragments arranged by size: 4593 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 BsmBI CGTCTCn'nnnn_ Cuts at: 2501 2501 Size: 5749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2483 2955 3068 3810 3895 4966 1391 Size: 399 693 472 113 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 693 472 399 113 85 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2863 2991 3104 844 Size: 2019 128 113 3489 Fragments arranged by size: 3489 2019 128 113 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2935 4244 4418 65 Size: 1183 1687 1309 174 1396 Fragments arranged by size: 1687 1396 1309 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 2154 2158 2745 4393 4580 1700 Size: 110 319 25 4 587 1648 187 2869 Fragments arranged by size: 2869 1648 587 319 187 110 25 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 2398 2930 794 Size: 1604 532 3613 Fragments arranged by size: 3613 1604 532 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstYI r'GATC_y Cuts at: 1972 2250 3079 3446 3831 3848 5300 5386 5398 Size: 278 829 367 385 17 1452 86 12 Cuts at: 5398 5744 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 829 385 367 346 278 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2499 2715 513 Size: 552 1434 216 3547 Fragments arranged by size: 3547 1434 552 216 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2628 487 Size: 683 56 1402 3608 Fragments arranged by size: 3608 1402 683 56 BtsI GCAGTG_nn' Cuts at: 2803 3265 4059 4079 2803 Size: 462 794 20 4473 Fragments arranged by size: 4473 794 462 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 2156 2319 Size: 54 1425 50 275 18 283 43 163 Cuts at: 2319 2469 2502 2647 3196 4085 5714 8 Size: 150 33 145 549 889 1629 43 Fragments arranged by size: 1629 1425 889 549 283 275 163 150 145 54 50 43 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2156 2502 3196 1537 Size: 275 344 346 694 4090 Fragments arranged by size: 4090 694 346 344 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3679 1353 Size: 2326 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2441 2770 4336 4710 4856 387 Size: 1064 138 852 329 1566 374 146 1280 Fragments arranged by size: 1566 1280 1064 852 374 329 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2263 2627 2708 2804 3812 4409 Size: 393 421 210 364 81 96 1008 597 Cuts at: 4409 5196 5464 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1008 787 597 421 393 364 268 210 96 81 EcoNI CCTnn'n_nnAGG Cuts at: 2288 2288 Size: 5749 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2047 2225 2303 2435 2715 2836 4896 1678 Size: 369 178 78 132 280 121 2060 2531 Fragments arranged by size: 2531 2060 369 280 178 132 121 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2612 2644 4390 4422 4464 Size: 32 135 32 1040 32 1746 32 42 Cuts at: 4464 4496 1373 Size: 32 2626 Fragments arranged by size: 2626 1746 1040 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KasI G'GCGC_C Cuts at: 2711 2711 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4863 5047 1305 Size: 3558 184 2007 Fragments arranged by size: 3558 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 2471 2649 10 Size: 54 1425 343 489 150 178 3110 Fragments arranged by size: 3110 1425 489 343 178 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 3707 4066 4225 518 Size: 648 978 1563 359 159 2042 Fragments arranged by size: 2042 1563 978 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2372 2398 2501 2709 2717 2779 3165 Size: 444 322 26 103 208 8 62 386 Cuts at: 3165 3842 4990 5235 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 444 386 322 245 208 103 62 26 8 NarI GG'CG_CC Cuts at: 2712 2712 Size: 5749 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5197 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 3107 4652 1132 Size: 97 1878 1545 2229 Fragments arranged by size: 2229 1878 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2686 1108 Size: 1578 4171 PfoI T'CCnGG_A Cuts at: 2957 2957 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2783 2815 3818 3850 5331 5363 2783 Size: 32 1003 32 1481 32 3169 Fragments arranged by size: 3169 1481 1003 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PstI C_TGCA'G Cuts at: 830 2217 2439 3101 830 Size: 1387 222 662 3478 Fragments arranged by size: 3478 1387 662 222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2779 2779 Size: 5749 SacII CC_GC'GG Cuts at: 2502 2718 2502 Size: 216 5533 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2213 2435 2623 3097 4232 4913 5104 Size: 219 1168 222 188 474 1135 681 191 Cuts at: 5104 826 Size: 1471 Fragments arranged by size: 1471 1168 1135 681 474 222 219 191 188 SfoI GGC'GCC Cuts at: 2713 2713 Size: 5749 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 2676 3076 1872 Size: 804 400 4545 Fragments arranged by size: 4545 804 400 SmlI C'TyrA_G Cuts at: 820 1017 1943 3853 4754 5016 820 Size: 197 926 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 3188 513 Size: 552 2123 3074 Fragments arranged by size: 3074 2123 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2843 3776 3793 3946 4131 4576 325 Size: 1255 1263 933 17 153 185 445 1498 Fragments arranged by size: 1498 1263 1255 933 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3995 5707 96 Size: 274 80 33 51 1443 2018 1712 138 Fragments arranged by size: 2018 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5709 5741 5709 Size: 32 5717 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 2674 3074 1870 Size: 804 400 4545 Fragments arranged by size: 4545 804 400 XmnI GAAnn'nnTTC Cuts at: 2798 3878 2798 Size: 1080 4669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI