(Circular) (Six-base) MAPSORT of: pFN21AB9707.seq Check: 235 from: 1 to: 5731 pFN21AB9707 5731 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3074 1937 Size: 1137 4594 Acc65I G'GTAC_C Cuts at: 2628 3052 2628 Size: 424 5307 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3794 5178 5446 1632 Size: 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 2952 4630 5607 1203 Size: 1749 1678 977 1327 Fragments arranged by size: 1749 1678 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2164 1184 Size: 980 4751 AlwNI CAG_nnn'CTG Cuts at: 2329 3474 5046 2329 Size: 1145 1572 3014 Fragments arranged by size: 3014 1572 1145 ApaI G_GGCC'C Cuts at: 2336 2357 2484 2559 2567 2336 Size: 21 127 75 8 5500 Fragments arranged by size: 5500 127 75 21 8 ApaLI G'TGCA_C Cuts at: 2290 3788 4944 2290 Size: 1498 1156 3077 Fragments arranged by size: 3077 1498 1156 ApoI r'AATT_y Cuts at: 1493 3040 3266 3418 5527 1493 Size: 1547 226 152 2109 1697 Fragments arranged by size: 2109 1697 1547 226 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2350 2590 3056 1870 Size: 73 407 240 466 4545 Fragments arranged by size: 4545 466 407 240 73 BamHI G'GATC_C Cuts at: 3061 3428 3061 Size: 367 5364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 2381 2609 2628 3052 Size: 325 217 627 528 66 228 19 424 Cuts at: 3052 4507 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 528 424 325 228 217 66 19 BanII G_rGCy'C Cuts at: 1391 2204 2244 2336 2357 2484 2559 2567 2639 Size: 813 40 92 21 127 75 8 72 Cuts at: 2639 2669 3050 1391 Size: 30 381 4072 Fragments arranged by size: 4072 813 381 127 92 75 72 40 30 21 8 BbeI G_GCGC'C Cuts at: 2319 2385 2319 Size: 66 5665 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4760 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3922 3956 1903 Size: 34 1985 34 3678 Fragments arranged by size: 3678 1985 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2690 4342 136 Size: 107 122 71 631 605 1018 1652 1525 Fragments arranged by size: 1652 1525 1018 631 605 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 2638 2888 3148 2638 Size: 250 260 5221 Fragments arranged by size: 5221 260 250 Bme1580I G_kGCm'C Cuts at: 1790 2294 2336 2357 2484 2559 2567 2979 3792 Size: 504 42 21 127 75 8 412 813 Cuts at: 3792 4948 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 813 504 412 127 75 42 21 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2029 2206 2509 Size: 416 53 83 399 24 612 177 303 Cuts at: 2509 3462 4420 442 Size: 953 958 1753 Fragments arranged by size: 1753 958 953 612 416 399 303 177 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2263 2440 2658 2263 Size: 177 218 5336 Fragments arranged by size: 5336 218 177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 2768 4391 1239 Size: 795 734 1623 2579 Fragments arranged by size: 2579 1623 795 734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3856 4721 5019 3856 Size: 865 298 4568 Fragments arranged by size: 4568 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2955 3522 493 Size: 711 1751 567 2702 Fragments arranged by size: 2702 1751 711 567 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2382 3538 3920 Size: 53 83 186 936 783 66 1156 382 Cuts at: 3920 4555 275 Size: 635 1451 Fragments arranged by size: 1451 1156 936 783 635 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 2992 4164 4562 4836 4983 5394 2992 Size: 1172 398 274 147 411 3329 Fragments arranged by size: 3329 1172 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 1178 Size: 30 165 30 783 30 4693 Fragments arranged by size: 4693 783 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5731 BseYI C'CCAG_C Cuts at: 2463 2978 4934 5430 2463 Size: 515 1956 496 2764 Fragments arranged by size: 2764 1956 515 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 1163 Size: 573 462 4696 Fragments arranged by size: 4696 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3181 3942 4970 1540 Size: 275 186 1180 761 1028 2301 Fragments arranged by size: 2301 1180 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2294 2639 3792 3877 4948 2294 Size: 345 1153 85 1071 3077 Fragments arranged by size: 3077 1153 1071 345 85 BsmI GAATG_Cn' Cuts at: 3242 3335 3242 Size: 93 5638 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2244 2294 2336 2357 2484 2559 Size: 399 414 40 50 42 21 127 75 Cuts at: 2559 2567 2639 2669 2979 3050 3792 3877 4948 Size: 8 72 30 310 71 742 85 1071 Cuts at: 4948 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 414 399 310 127 85 75 72 71 50 42 40 30 21 8 BspEI T'CCGG_A Cuts at: 2992 5394 2992 Size: 2402 3329 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3086 844 Size: 2242 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2313 2937 3618 1394 Size: 429 490 624 681 3507 Fragments arranged by size: 3507 681 624 490 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4226 4400 65 Size: 1183 2978 174 1396 Fragments arranged by size: 2978 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2369 4375 4562 1700 Size: 110 559 2006 187 2869 Fragments arranged by size: 2869 2006 559 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5731 BssHII G'CGCG_C Cuts at: 1925 2434 1925 Size: 509 5222 BssSI C'ACGA_G Cuts at: 2425 3791 4803 2425 Size: 1366 1012 3353 Fragments arranged by size: 3353 1366 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstXI CCAn_nnnn'nTGG Cuts at: 2833 2833 Size: 5731 BstYI r'GATC_y Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726 Size: 1089 367 385 17 1452 86 12 346 Cuts at: 5726 1972 Size: 1977 Fragments arranged by size: 1977 1452 1089 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2228 2261 2385 2406 2559 513 Size: 552 939 224 33 124 21 153 3685 Fragments arranged by size: 3685 939 552 224 153 124 33 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4992 Fragments arranged by size: 4992 683 56 BtsI GCAGTG_nn' Cuts at: 2512 3247 4041 4061 2512 Size: 735 794 20 4182 Fragments arranged by size: 4182 794 735 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DraIII CAC_nnn'GTG Cuts at: 2427 2427 Size: 5731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696 Size: 54 1425 50 275 18 1348 889 1629 Cuts at: 5696 8 Size: 43 Fragments arranged by size: 1629 1425 1348 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 3661 1353 Size: 909 1399 3423 Fragments arranged by size: 3423 1399 909 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2252 3024 4318 4692 4838 387 Size: 1064 138 663 772 1294 374 146 1280 Fragments arranged by size: 1294 1280 1064 772 663 374 146 138 EcoICRI GAG'CTC Cuts at: 2637 2637 Size: 5731 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2768 3794 4391 5178 5446 1239 Size: 393 402 734 1026 597 787 268 1524 Fragments arranged by size: 1524 1026 787 734 597 402 393 268 EcoO109I rG'GnC_Cy Cuts at: 2333 2354 2480 2720 2896 2333 Size: 21 126 240 176 5168 Fragments arranged by size: 5168 240 176 126 21 EcoRI G'AATT_C Cuts at: 3040 3418 3040 Size: 378 5353 FspI TGC'GCA Cuts at: 2930 4237 2930 Size: 1307 4424 HaeII r_GCGC'y Cuts at: 1678 2319 2385 4878 1678 Size: 641 66 2493 2531 Fragments arranged by size: 2531 2493 641 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2186 2218 4372 Size: 32 135 32 443 32 139 32 2154 Cuts at: 4372 4404 4446 4478 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2154 443 139 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 2179 748 Size: 1431 4300 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KasI G'GCGC_C Cuts at: 2315 2381 2315 Size: 66 5665 KpnI G_GTAC'C Cuts at: 2632 3056 2632 Size: 424 5307 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 2768 4845 5029 1305 Size: 1350 113 2077 184 2007 Fragments arranged by size: 2077 2007 1350 184 113 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3909 Fragments arranged by size: 3909 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2604 3689 4048 4207 518 Size: 648 1438 1085 359 159 2042 Fragments arranged by size: 2042 1438 1085 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2387 2463 2561 2731 2778 2815 3147 Size: 585 196 76 98 170 47 37 332 Cuts at: 3147 3824 4972 5217 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 585 332 245 196 170 98 76 47 37 NaeI GCC'GGC Cuts at: 2371 2371 Size: 5731 NarI GG'CG_CC Cuts at: 2316 2382 2316 Size: 66 5665 NcoI C'CATG_G Cuts at: 513 1065 2004 2228 513 Size: 552 939 224 4016 Fragments arranged by size: 4016 939 552 224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NgoMIV G'CCGG_C Cuts at: 2369 2369 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2151 2603 3089 4634 1132 Size: 97 922 452 486 1545 2229 Fragments arranged by size: 2229 1545 922 486 452 97 PasI CC'CwG_GG Cuts at: 1348 2329 1348 Size: 981 4750 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 2427 2547 2583 1108 Size: 1319 120 36 4256 Fragments arranged by size: 4256 1319 120 36 PfoI T'CCnGG_A Cuts at: 2582 2745 2582 Size: 163 5568 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2720 2720 Size: 5731 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PspOMI G'GGCC_C Cuts at: 2332 2353 2480 2555 2563 2332 Size: 21 127 75 8 5500 Fragments arranged by size: 5500 127 75 21 8 PstI C_TGCA'G Cuts at: 830 3083 830 Size: 2253 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 2191 2463 2731 2778 2815 2191 Size: 272 268 47 37 5107 Fragments arranged by size: 5107 272 268 47 37 RsrII CG'GwC_CG Cuts at: 2677 2677 Size: 5731 SacI G_AGCT'C Cuts at: 2639 2639 Size: 5731 SacII CC_GC'GG Cuts at: 2388 2562 2388 Size: 174 5557 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SapI GCTCTTCn'nnn_ Cuts at: 2262 2262 Size: 5731 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 3079 4214 4895 5086 826 Size: 219 2034 1135 681 191 1471 Fragments arranged by size: 2034 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2317 2383 2317 Size: 66 5665 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 2352 2592 3058 1872 Size: 480 240 466 4545 Fragments arranged by size: 4545 480 466 240 SmlI C'TyrA_G Cuts at: 820 1017 1943 3835 4736 4998 820 Size: 197 926 1892 901 262 1553 Fragments arranged by size: 1892 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 2603 3089 1132 Size: 1471 486 3774 Fragments arranged by size: 3774 1471 486 SrfI GCCC'GGGC Cuts at: 2352 2352 Size: 5731 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5731 StyI C'CwwG_G Cuts at: 513 1065 2004 2116 2228 3170 513 Size: 552 939 112 112 942 3074 Fragments arranged by size: 3074 942 939 552 112 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2232 3758 3775 3928 4113 4558 325 Size: 1255 652 1526 17 153 185 445 1498 Fragments arranged by size: 1526 1498 1255 652 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2949 3977 5689 Size: 274 80 33 51 1443 972 1028 1712 Cuts at: 5689 96 Size: 138 Fragments arranged by size: 1712 1443 1028 972 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5691 5723 5691 Size: 32 5699 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2193 2226 2193 Size: 33 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 2350 2590 3056 1870 Size: 480 240 466 4545 Fragments arranged by size: 4545 480 466 240 XmnI GAAnn'nnTTC Cuts at: 3860 3860 Size: 5731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SanDI SexAI SfiI SwaI