Restriction map for
pFN21AB9705
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AseI AsnI Asp700I AspHI
AspI AviII AvrII BalI BamHI
BbsI BbvCI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
HphI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NdeI NdeII NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI Sau3AI
SbfI ScaI SexAI SfaNI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 659 GGTACC G^GTACC
AccI 790 GTCTAC GT^[AC][GT]AC
AcsI 871 AAATTC [GA]^AATT[CT]
AhdI 598 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 871 AAATTC [GA]^AATT[CT]
AscI 264 GGCGCGCC GG^CGCGCC
Asp718I 659 GGTACC G^GTACC
AspEI 598 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 580 CACGTG CAC^GTG
BciVI 544 GTATCC GTATCC(6/5)
BsaAI 580 CACGTG [CT]AC^GT[GA]
BsgI 705 CTGCAC GTGCAG(16/14)
BsiEI 317 CGGCCG CG[GA][CT]^CG
BspLU11I 782 ACATGT A^CATGT
BssHII 265 GCGCGC G^CGCGC
BssSI 634 CACGAG CACGAG(-5/-1)
BstF5I 759 GGATG GGATG(2/0)
DraIII 400 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 781 GACATGTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 317 CGGCCG C^GGCCG
Eam1105I 598 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 317 CGGCCG C^GGCCG
Eco52I 317 CGGCCG C^GGCCG
FokI 759 GGATG GGATG(9/13)
KpnI 659 GGTACC GGTAC^C
KspI 96 CCGCGG CCGC^GG
MaeIII 934 GTGAC ^GT[ACGT]AC
NcoI 650 CCATGG C^CATGG
NotI 316 GCGGCCGC GC^GGCCGC
NspI 782 ACATGT [GA]CATG^[CT]
PmaCI 580 CACGTG CAC^GTG
PmlI 580 CACGTG CAC^GTG
PvuII 384 CAGCTG CAG^CTG
SacII 96 CCGCGG CCGC^GG
SapI 227 GCTCTTC GCTCTTC(1/4)
SgrAI 30 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 205 CCCGGG CCC^GGG
SmlI 427 CTCAAG C^T[CT][GA]AG
TaqI 596 TCGA T^CGA
Tsp45I 934 GTGAC ^GT[GC]AC
TspRI 91 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 596 TCGA T^CGA
XmaCI 205 CCCGGG C^CCGGG
XmaI 205 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 482 ACGTGT A^C[GA][CT]GT
782 ACATGT
Aor51HI 337 AGCGCT AGC^GCT
345 AGCGCT
AvaI 205 CCCGGG C^[CT]CG[GA]G
333 CCCGAG
BanII 225 GGGCTC G[GA]GC[CT]^C
567 GAGCCC
BbeI 28 GGCGCC GGCGC^C
37 GGCGCC
BpmI 341 CTCCAG CTGGAG(16/14)
847 CTGGAG
BseRI 54 CTCCTC GAGGAG(10/8)
57 CTCCTC
BsmI 420 GAATGC GAATGC(1/-1)
132 GCATTC
BsoBI 205 CCCGGG C^[CT]CG[GA]G
333 CCCGAG
BsrFI 31 GCCGGT [GA]^CCGG[CT]
510 ACCGGC
Cfr10I 31 GCCGGT [GA]^CCGG[CT]
510 ACCGGC
DdeI 215 CTGAG C^T[ACGT]AG
923 CTGAG
DraII 208 GGGACCT [GA]G^G[ACGT]CC[CT]
727 GGGTCCC
EaeI 317 CGGCCG [CT]^GGCC[GA]
512 CGGCCA
Eco47III 337 AGCGCT AGC^GCT
345 AGCGCT
EcoO109I 208 GGGACCT [GA]G^G[ACGT]CC[CT]
727 GGGTCCC
KasI 28 GGCGCC G^GCGCC
37 GGCGCC
MaeII 482 ACGT A^CGT
581 ACGT
MslI 477 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
645 CATCTCCATG
NarI 28 GGCGCC GG^CGCC
37 GGCGCC
PpuMI 208 GGGACCT [GA]G^G[AT]CC[CT]
727 GGGTCCC
SfoI 28 GGCGCC GGC^GCC
37 GGCGCC
StuI 489 AGGCCT AGG^CCT
638 AGGCCT
Tsp509I 872 AATT ^AATT
975 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DsaI
EarI EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI Ksp632I
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PstI RsaI Sau96I ScrFI
SfcI StyI Tru9I TseI