(Circular) (Six-base) MAPSORT of: pFN21AB9705.seq Check: 8896 from: 1 to: 5710 pFN21AB9705 5710 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2796 3053 1937 Size: 859 257 4594 Fragments arranged by size: 4594 859 257 Acc65I G'GTAC_C Cuts at: 2664 3031 2664 Size: 367 5343 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3773 5157 5425 1632 Size: 2141 1384 268 1917 Fragments arranged by size: 2141 1917 1384 268 AfeI AGC'GCT Cuts at: 2344 2352 2344 Size: 8 5702 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 2487 2787 4609 5586 1203 Size: 1284 300 1822 977 1327 Fragments arranged by size: 1822 1327 1284 977 300 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 2608 4439 2608 Size: 1831 3879 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 3453 5025 3453 Size: 1572 4138 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2876 3019 3245 3397 5506 1493 Size: 1383 143 226 152 2109 1697 Fragments arranged by size: 2109 1697 1383 226 152 143 AscI GG'CGCG_CC Cuts at: 2270 2270 Size: 5710 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2210 2338 3035 1870 Size: 73 267 128 697 4545 Fragments arranged by size: 4545 697 267 128 73 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2042 2527 2664 3031 Size: 325 217 627 246 9 485 137 367 Cuts at: 3031 4486 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 485 367 325 246 217 137 9 BanII G_rGCy'C Cuts at: 1391 2234 2576 3029 1391 Size: 843 342 453 4072 Fragments arranged by size: 4072 843 453 342 BbeI G_GCGC'C Cuts at: 2037 2046 2037 Size: 9 5701 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2560 3602 4812 2560 Size: 1042 1210 3458 Fragments arranged by size: 3458 1210 1042 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2303 2592 3771 4927 1790 Size: 513 289 1179 1156 2573 Fragments arranged by size: 2573 1179 1156 513 289 BmgBI CAC'GTC Cuts at: 1318 2488 1318 Size: 1170 4540 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2331 2873 4370 1239 Size: 1092 542 1497 2579 Fragments arranged by size: 2579 1497 1092 542 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2417 3835 4700 4998 2417 Size: 1418 865 298 3129 Fragments arranged by size: 3129 1418 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2587 3501 493 Size: 711 1383 914 2702 Fragments arranged by size: 2702 1383 914 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2043 2087 3517 Size: 53 83 186 936 501 9 44 1430 Cuts at: 3517 3899 4534 275 Size: 382 635 1451 Fragments arranged by size: 1451 1430 936 635 501 382 186 83 53 44 9 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2846 2876 1178 Size: 30 165 30 1443 30 4012 Fragments arranged by size: 4012 1443 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2050 2053 1186 Size: 864 3 4843 Fragments arranged by size: 4843 864 3 BseYI C'CCAG_C Cuts at: 2287 2302 2353 2455 4913 5409 2287 Size: 15 51 102 2458 496 2588 Fragments arranged by size: 2588 2458 496 102 51 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2695 1163 Size: 573 959 4178 Fragments arranged by size: 4178 959 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2325 3160 3921 4949 1540 Size: 275 186 324 835 761 1028 2301 Fragments arranged by size: 2301 1028 835 761 324 275 186 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 2137 2431 3221 3314 2137 Size: 294 790 93 4533 Fragments arranged by size: 4533 790 294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2303 2576 2592 3029 3771 3856 Size: 399 444 69 273 16 437 742 85 Cuts at: 3856 4927 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 444 437 399 273 85 69 16 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3065 844 Size: 2221 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 2515 4354 4541 1700 Size: 110 226 479 1839 187 2869 Fragments arranged by size: 2869 1839 479 226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 2270 1925 Size: 345 5365 BssSI C'ACGA_G Cuts at: 2639 3770 4782 2639 Size: 1131 1012 3567 Fragments arranged by size: 3567 1131 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstYI r'GATC_y Cuts at: 1972 3040 3407 3792 3809 5261 5347 5359 5705 Size: 1068 367 385 17 1452 86 12 346 Cuts at: 5705 1972 Size: 1977 Fragments arranged by size: 1977 1452 1068 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2101 2469 2655 513 Size: 552 939 97 368 186 3568 Fragments arranged by size: 3568 939 552 368 186 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2424 487 Size: 683 56 1198 3773 Fragments arranged by size: 3773 1198 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DraIII CAC_nnn'GTG Cuts at: 2410 2410 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2792 4717 809 Size: 507 1476 1925 1802 Fragments arranged by size: 1925 1802 1476 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2517 3157 Size: 54 1425 50 275 18 492 195 640 Cuts at: 3157 4046 5675 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 640 492 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2322 3157 1537 Size: 275 510 835 4090 Fragments arranged by size: 4090 835 510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2059 2239 2257 2797 2810 2956 3640 1353 Size: 706 180 18 540 13 146 684 3423 Fragments arranged by size: 3423 706 684 540 180 146 18 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2873 3773 4370 5157 5425 1239 Size: 393 699 542 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 699 597 542 393 268 EcoO109I rG'GnC_Cy Cuts at: 2214 2733 2214 Size: 519 5191 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2037 2046 2336 2346 2354 4857 1678 Size: 359 9 290 10 8 2503 2531 Fragments arranged by size: 2531 2503 359 290 10 9 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2594 2626 2990 3022 4351 Size: 32 135 32 1022 32 364 32 1329 Cuts at: 4351 4383 4425 4457 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1329 1022 364 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KasI G'GCGC_C Cuts at: 2033 2042 2033 Size: 9 5701 KpnI G_GTAC'C Cuts at: 2668 3035 2668 Size: 367 5343 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 2654 3668 4027 4186 518 Size: 648 1320 168 1014 359 159 2042 Fragments arranged by size: 2042 1320 1014 648 359 168 159 MspA1I CmG'CkG Cuts at: 1606 2103 2165 2186 2321 2327 2391 3126 3803 Size: 497 62 21 135 6 64 735 677 Cuts at: 3803 4951 5196 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 735 677 497 245 135 64 62 21 6 NarI GG'CG_CC Cuts at: 2034 2043 2034 Size: 9 5701 NcoI C'CATG_G Cuts at: 513 1065 2004 2655 513 Size: 552 939 651 3568 Fragments arranged by size: 3568 939 651 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 2322 3157 2322 Size: 835 4875 NspI r_CATG'y Cuts at: 1132 1229 2791 3068 4613 1132 Size: 97 1562 277 1545 2229 Fragments arranged by size: 2229 1562 1545 277 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 2787 4609 2787 Size: 1822 3888 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PfoI T'CCnGG_A Cuts at: 2672 2672 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PmlI CAC'GTG Cuts at: 2587 2587 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2214 2733 2214 Size: 519 5191 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 2320 2332 2841 3062 830 Size: 1490 12 509 221 3478 Fragments arranged by size: 3478 1490 509 221 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2391 2391 Size: 5710 SacII CC_GC'GG Cuts at: 2104 2104 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SanDI GG'GwC_CC Cuts at: 2733 2733 Size: 5710 SapI GCTCTTCn'nnn_ Cuts at: 2239 2239 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2316 2328 2386 2837 3058 4193 4874 Size: 219 1271 12 58 451 221 1135 681 Cuts at: 4874 5065 826 Size: 191 1471 Fragments arranged by size: 1471 1271 1135 681 451 221 219 191 58 12 SfoI GGC'GCC Cuts at: 2035 2044 2035 Size: 9 5701 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 2036 1700 Size: 336 5374 SmaI CCC'GGG Cuts at: 1872 2212 3037 1872 Size: 340 825 4545 Fragments arranged by size: 4545 825 340 SmlI C'TyrA_G Cuts at: 820 1017 1943 2432 3814 4715 4977 820 Size: 197 926 489 1382 901 262 1553 Fragments arranged by size: 1553 1382 926 901 489 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 2496 2645 1517 Size: 979 149 4582 Fragments arranged by size: 4582 979 149 StyI C'CwwG_G Cuts at: 513 1065 2004 2541 2655 2715 3149 513 Size: 552 939 537 114 60 434 3074 Fragments arranged by size: 3074 939 552 537 434 114 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2323 3737 3754 3907 4092 4537 325 Size: 1255 743 1414 17 153 185 445 1498 Fragments arranged by size: 1498 1414 1255 743 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2478 2706 3956 Size: 274 80 33 51 1443 501 228 1250 Cuts at: 3956 5668 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1250 501 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 2210 3035 1870 Size: 340 825 4545 Fragments arranged by size: 4545 825 340 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI XcmI