Restriction map for
pFN21AB9705
(Linear) (Six-base) MAPSORT of: KIBB9705.seq Check: 8384 from: 1 to: 1005
KIBB9705.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 791 1005
Size: 791 214
Acc65I G'GTAC_C
Cuts at: 0 659 1005
Size: 659 346
AfeI AGC'GCT
Cuts at: 0 339 347 1005
Size: 339 8 658
Fragments arranged by size:
658 339 8
AflIII A'CryG_T
Cuts at: 0 482 782 1005
Size: 482 300 223
Fragments arranged by size:
482 300 223
AhdI GACnn_n'nnGTC
Cuts at: 0 603 1005
Size: 603 402
ApoI r'AATT_y
Cuts at: 0 871 1005
Size: 871 134
AscI GG'CGCG_CC
Cuts at: 0 265 1005
Size: 265 740
AvaI C'yCGr_G
Cuts at: 0 205 333 1005
Size: 205 128 672
Fragments arranged by size:
672 205 128
BanI G'GyrC_C
Cuts at: 0 28 37 522 659 1005
Size: 28 9 485 137 346
Fragments arranged by size:
485 346 137 28 9
BanII G_rGCy'C
Cuts at: 0 229 571 1005
Size: 229 342 434
Fragments arranged by size:
434 342 229
BbeI G_GCGC'C
Cuts at: 0 32 41 1005
Size: 32 9 964
Fragments arranged by size:
964 32 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 555 1005
Size: 555 450
Bme1580I G_kGCm'C
Cuts at: 0 298 587 1005
Size: 298 289 418
Fragments arranged by size:
418 298 289
BmgBI CAC'GTC
Cuts at: 0 483 1005
Size: 483 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 868 1005
Size: 326 542 137
Fragments arranged by size:
542 326 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1005
Size: 412 593
BsaAI yAC'GTr
Cuts at: 0 582 1005
Size: 582 423
BsaHI Gr'CG_yC
Cuts at: 0 29 38 82 1005
Size: 29 9 44 923
Fragments arranged by size:
923 44 29 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 841 871 1005
Size: 841 30 134
Fragments arranged by size:
841 134 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 48 1005
Size: 45 3 957
Fragments arranged by size:
957 45 3
BseYI C'CCAG_C
Cuts at: 0 282 297 348 450 1005
Size: 282 15 51 102 555
Fragments arranged by size:
555 282 102 51 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 1005
Size: 690 315
BsiEI CG_ry'CG
Cuts at: 0 320 1005
Size: 320 685
BsmI GAATG_Cn'
Cuts at: 0 132 426 1005
Size: 132 294 579
Fragments arranged by size:
579 294 132
Bsp1286I G_dGCh'C
Cuts at: 0 229 298 571 587 1005
Size: 229 69 273 16 418
Fragments arranged by size:
418 273 229 69 16
BsrFI r'CCGG_y
Cuts at: 0 31 510 1005
Size: 31 479 495
Fragments arranged by size:
495 479 31
BssHII G'CGCG_C
Cuts at: 0 265 1005
Size: 265 740
BssSI C'ACGA_G
Cuts at: 0 634 1005
Size: 634 371
BtgI C'CryG_G
Cuts at: 0 96 464 650 1005
Size: 96 368 186 355
Fragments arranged by size:
368 355 186 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 419 1005
Size: 419 586
DraIII CAC_nnn'GTG
Cuts at: 0 405 1005
Size: 405 600
DrdI GACnn_nn'nnGTC
Cuts at: 0 787 1005
Size: 787 218
EaeI y'GGCC_r
Cuts at: 0 317 512 1005
Size: 317 195 493
Fragments arranged by size:
493 317 195
EagI C'GGCC_G
Cuts at: 0 317 1005
Size: 317 688
EarI CTCTTCn'nnn_
Cuts at: 0 54 234 252 792 805 951 1005
Size: 54 180 18 540 13 146 54
Fragments arranged by size:
540 180 146 54 54 18 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 868 1005
Size: 326 542 137
Fragments arranged by size:
542 326 137
EcoO109I rG'GnC_Cy
Cuts at: 0 209 728 1005
Size: 209 519 277
Fragments arranged by size:
519 277 209
HaeII r_GCGC'y
Cuts at: 0 32 41 331 341 349 1005
Size: 32 9 290 10 8 656
Fragments arranged by size:
656 290 32 10 9 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 985 1005
Size: 589 32 364 20
Fragments arranged by size:
589 364 32 20
KasI G'GCGC_C
Cuts at: 0 28 37 1005
Size: 28 9 968
Fragments arranged by size:
968 28 9
KpnI G_GTAC'C
Cuts at: 0 663 1005
Size: 663 342
MslI CAynn'nnrTG
Cuts at: 0 481 649 1005
Size: 481 168 356
Fragments arranged by size:
481 356 168
MspA1I CmG'CkG
Cuts at: 0 98 160 181 316 322 386 1005
Size: 98 62 21 135 6 64 619
Fragments arranged by size:
619 135 98 64 62 21 6
NarI GG'CG_CC
Cuts at: 0 29 38 1005
Size: 29 9 967
Fragments arranged by size:
967 29 9
NcoI C'CATG_G
Cuts at: 0 650 1005
Size: 650 355
NotI GC'GGCC_GC
Cuts at: 0 317 1005
Size: 317 688
NspI r_CATG'y
Cuts at: 0 786 1005
Size: 786 219
PciI A'CATG_T
Cuts at: 0 782 1005
Size: 782 223
PfoI T'CCnGG_A
Cuts at: 0 667 1005
Size: 667 338
PmlI CAC'GTG
Cuts at: 0 582 1005
Size: 582 423
PpuMI rG'GwC_Cy
Cuts at: 0 209 728 1005
Size: 209 519 277
Fragments arranged by size:
519 277 209
PstI C_TGCA'G
Cuts at: 0 315 327 836 1005
Size: 315 12 509 169
Fragments arranged by size:
509 315 169 12
PvuII CAG'CTG
Cuts at: 0 386 1005
Size: 386 619
SacII CC_GC'GG
Cuts at: 0 99 1005
Size: 99 906
SanDI GG'GwC_CC
Cuts at: 0 728 1005
Size: 728 277
SapI GCTCTTCn'nnn_
Cuts at: 0 234 1005
Size: 234 771
SfcI C'TryA_G
Cuts at: 0 311 323 381 832 1005
Size: 311 12 58 451 173
Fragments arranged by size:
451 311 173 58 12
SfoI GGC'GCC
Cuts at: 0 30 39 1005
Size: 30 9 966
Fragments arranged by size:
966 30 9
SgrAI Cr'CCGG_yG
Cuts at: 0 31 1005
Size: 31 974
SmaI CCC'GGG
Cuts at: 0 207 1005
Size: 207 798
SmlI C'TyrA_G
Cuts at: 0 427 1005
Size: 427 578
StuI AGG'CCT
Cuts at: 0 491 640 1005
Size: 491 149 365
Fragments arranged by size:
491 365 149
StyI C'CwwG_G
Cuts at: 0 536 650 710 1005
Size: 536 114 60 295
Fragments arranged by size:
536 295 114 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 318 1005
Size: 318 687
TatI w'GTAC_w
Cuts at: 0 473 701 1005
Size: 473 228 304
Fragments arranged by size:
473 304 228
XmaI C'CCGG_G
Cuts at: 0 205 1005
Size: 205 800
Enzymes that do cut:
AccI Acc65I AfeI AflIII AhdI ApoI AscI AvaI
BanI BanII BbeI BciVI Bme1580I BmgBI BpmI BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI
Bsp1286I BsrFI BssHII BssSI BtgI BtgZI DraIII DrdI
EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I KasI
KpnI MslI MspA1I NarI NcoI NotI NspI PciI
PfoI PmlI PpuMI PstI PvuII SacII SanDI SapI
SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaWI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI
NruI NsiI PacI PasI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI