(Circular) (Six-base) MAPSORT of: pFN21AB9695.seq Check: 779 from: 1 to: 5497 pFN21AB9695 5497 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2563 2563 Size: 5497 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3307 278 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2840 1937 Size: 903 4594 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3560 4944 5212 1632 Size: 1928 1384 268 1917 Fragments arranged by size: 1928 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 4396 5373 1203 Size: 3193 977 1327 Fragments arranged by size: 3193 1327 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2468 2495 1184 Size: 1284 27 4186 Fragments arranged by size: 4186 1284 27 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2483 2515 2483 Size: 32 5465 AlwNI CAG_nnn'CTG Cuts at: 3240 4812 3240 Size: 1572 3925 ApaI G_GGCC'C Cuts at: 2214 2214 Size: 5497 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2806 3032 3184 5293 1493 Size: 1313 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2216 2315 2822 1870 Size: 73 123 150 99 507 4545 Fragments arranged by size: 4545 507 150 123 99 73 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 2138 2657 2818 4273 Size: 325 217 627 275 76 519 161 1455 Cuts at: 4273 618 Size: 1842 Fragments arranged by size: 1842 1455 627 519 325 275 217 161 76 BanII G_rGCy'C Cuts at: 1391 2214 2816 1391 Size: 823 602 4072 Fragments arranged by size: 4072 823 602 BbeI G_GCGC'C Cuts at: 2066 2142 2066 Size: 76 5421 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4526 BbvCI CC'TCA_GC Cuts at: 2710 2710 Size: 5497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2524 2558 3688 3722 1903 Size: 34 50 34 503 34 1130 34 3678 Fragments arranged by size: 3678 1130 503 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3389 4599 3389 Size: 1210 4287 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2218 4108 136 Size: 107 122 71 631 605 546 1890 1525 Fragments arranged by size: 1890 1525 631 605 546 122 107 71 BglII A'GATC_T Cuts at: 2580 5492 2580 Size: 2912 2585 BlpI GC'TnA_GC Cuts at: 2914 2914 Size: 5497 Bme1580I G_kGCm'C Cuts at: 1790 2106 2180 2214 3558 4714 1790 Size: 316 74 34 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 316 74 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3228 4186 442 Size: 416 53 83 399 24 1811 958 1753 Fragments arranged by size: 1811 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2510 4157 1239 Size: 1271 1647 2579 Fragments arranged by size: 2579 1647 1271 Bpu10I CC'TnA_GC Cuts at: 2710 2710 Size: 5497 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2225 3622 4487 4785 2225 Size: 1397 865 298 2937 Fragments arranged by size: 2937 1397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2132 4160 882 Size: 617 633 2028 2219 Fragments arranged by size: 2219 2028 633 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2063 2139 3304 3686 Size: 53 83 186 936 530 76 1165 382 Cuts at: 3686 4321 275 Size: 635 1451 Fragments arranged by size: 1451 1165 936 635 530 382 186 83 76 53 BsaWI w'CCGG_w Cuts at: 3930 4328 4602 4749 5160 3930 Size: 398 274 147 411 4267 Fragments arranged by size: 4267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2354 2384 2483 2513 2776 Size: 30 165 30 951 30 99 30 263 Cuts at: 2776 2806 1178 Size: 30 3869 Fragments arranged by size: 3869 951 263 165 99 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2698 2749 1186 Size: 1512 51 3934 Fragments arranged by size: 3934 1512 51 BseYI C'CCAG_C Cuts at: 2159 2438 4700 5196 2159 Size: 279 2262 496 2460 Fragments arranged by size: 2460 2262 496 279 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 1163 Size: 573 369 4555 Fragments arranged by size: 4555 573 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2337 2776 2947 3708 4736 1540 Size: 275 186 336 439 171 761 1028 2301 Fragments arranged by size: 2301 1028 761 439 336 275 186 171 BsiHKAI G_wGCw'C Cuts at: 2723 3558 3643 4714 2723 Size: 835 85 1071 3506 Fragments arranged by size: 3506 1071 835 85 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2180 2214 2723 2816 3558 3643 Size: 399 316 74 34 509 93 742 85 Cuts at: 3643 4714 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 509 399 316 93 85 74 34 BspEI T'CCGG_A Cuts at: 5160 5160 Size: 5497 BspHI T'CATG_A Cuts at: 3386 5116 3386 Size: 1730 3767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2563 2852 844 Size: 1719 289 3489 Fragments arranged by size: 3489 1719 289 BsrBI CCG'CTC Cuts at: 1394 1823 2086 2182 3384 1394 Size: 429 263 96 1202 3507 Fragments arranged by size: 3507 1202 429 263 96 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2660 4141 4328 1700 Size: 110 850 1481 187 2869 Fragments arranged by size: 2869 1481 850 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 2150 1925 Size: 225 5272 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 2119 794 Size: 1325 4172 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2580 2779 2827 3194 3579 3596 5048 5134 Size: 608 199 48 367 385 17 1452 86 Cuts at: 5134 5146 5492 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 608 385 367 346 199 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 2761 2761 Size: 5497 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4945 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2685 487 Size: 683 56 1459 3299 Fragments arranged by size: 3299 1459 683 56 BtsI GCAGTG_nn' Cuts at: 2401 3013 3807 3827 2401 Size: 612 794 20 4071 Fragments arranged by size: 4071 794 612 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2620 2655 529 Size: 35 2056 35 3371 Fragments arranged by size: 3371 2056 35 35 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 2944 3833 Size: 54 1425 50 275 18 504 610 889 Cuts at: 3833 5462 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 610 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2334 2944 1537 Size: 275 522 610 4090 Fragments arranged by size: 4090 610 522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 3427 1353 Size: 740 1334 3423 Fragments arranged by size: 3423 1334 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4084 4458 4604 387 Size: 1064 138 2495 374 146 1280 Fragments arranged by size: 2495 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 3560 4157 4944 5212 1239 Size: 393 878 1050 597 787 268 1524 Fragments arranged by size: 1524 1050 878 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2211 2211 Size: 5497 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2266 2266 Size: 5497 FspI TGC'GCA Cuts at: 2456 2698 4003 2456 Size: 242 1305 3950 Fragments arranged by size: 3950 1305 242 FspAI rTGC'GCAy Cuts at: 2456 2698 2456 Size: 242 5255 HaeII r_GCGC'y Cuts at: 1678 2066 2142 4644 1678 Size: 388 76 2502 2531 Fragments arranged by size: 2531 2502 388 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2408 2440 4138 4170 4212 Size: 32 135 32 836 32 1698 32 42 Cuts at: 4212 4244 1373 Size: 32 2626 Fragments arranged by size: 2626 1698 836 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2841 3087 669 Size: 988 281 903 246 3079 Fragments arranged by size: 3079 988 903 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KasI G'GCGC_C Cuts at: 2062 2138 2062 Size: 76 5421 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4611 4795 1305 Size: 3306 184 2007 Fragments arranged by size: 3306 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3675 Fragments arranged by size: 3675 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 3455 3814 3973 518 Size: 648 1245 1044 359 159 2042 Fragments arranged by size: 2042 1245 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2203 2342 2380 2434 2479 2913 3590 4738 Size: 597 139 38 54 45 434 677 1148 Cuts at: 4738 4983 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 597 434 245 139 54 45 38 NarI GG'CG_CC Cuts at: 2063 2139 2063 Size: 76 5421 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4945 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NruI TCG'CGA Cuts at: 2546 2546 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2855 4400 1132 Size: 97 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 97 PasI CC'CwG_GG Cuts at: 1348 2287 1348 Size: 939 4558 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5497 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2483 2515 3566 3598 5079 5111 2483 Size: 32 1051 32 1481 32 2869 Fragments arranged by size: 2869 1481 1051 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PspOMI G'GGCC_C Cuts at: 2210 2210 Size: 5497 PstI C_TGCA'G Cuts at: 830 2849 830 Size: 2019 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 PvuII CAG'CTG Cuts at: 2203 2434 2479 2203 Size: 231 45 5221 Fragments arranged by size: 5221 231 45 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SapI GCTCTTCn'nnn_ Cuts at: 2093 2093 Size: 5497 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 2273 3745 5457 2273 Size: 1472 1712 2313 Fragments arranged by size: 2313 1712 1472 SfcI C'TryA_G Cuts at: 826 1045 2845 3980 4661 4852 826 Size: 219 1800 1135 681 191 1471 Fragments arranged by size: 1800 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2218 2218 Size: 5497 SfoI GGC'GCC Cuts at: 2064 2140 2064 Size: 76 5421 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2218 2317 2824 1872 Size: 346 99 507 4545 Fragments arranged by size: 4545 507 346 99 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 3601 4502 4764 820 Size: 197 926 297 1361 901 262 1553 Fragments arranged by size: 1553 1361 926 901 297 262 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5497 StyI C'CwwG_G Cuts at: 513 1065 2936 513 Size: 552 1871 3074 Fragments arranged by size: 3074 1871 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3524 3541 3694 3879 4324 325 Size: 1255 1944 17 153 185 445 1498 Fragments arranged by size: 1944 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 3743 5455 Size: 274 80 33 51 1443 294 1472 1712 Cuts at: 5455 96 Size: 138 Fragments arranged by size: 1712 1472 1443 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5457 5489 5457 Size: 32 5465 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5497 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5497 XmaI C'CCGG_G Cuts at: 1870 2216 2315 2822 1870 Size: 346 99 507 4545 Fragments arranged by size: 4545 507 346 99 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3305 276 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI NaeI NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SrfI SwaI XcmI