(Circular) (Six-base) MAPSORT of: pFN21AB9691.seq Check: 9456 from: 1 to: 5395 pFN21AB9691 5395 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3205 278 Size: 53 83 186 936 1669 2468 Fragments arranged by size: 2468 1669 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2738 1937 Size: 801 4594 Acc65I G'GTAC_C Cuts at: 2716 2716 Size: 5395 AclI AA'CG_TT Cuts at: 1563 3522 3895 1563 Size: 1959 373 3063 Fragments arranged by size: 3063 1959 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2364 3458 4842 5110 1632 Size: 732 1094 1384 268 1917 Fragments arranged by size: 1917 1384 1094 732 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5198 AflIII A'CryG_T Cuts at: 1203 4294 5271 1203 Size: 3091 977 1327 Fragments arranged by size: 3091 1327 977 AgeI A'CCGG_T Cuts at: 4226 4226 Size: 5395 AhdI GACnn_n'nnGTC Cuts at: 4124 4124 Size: 5395 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5395 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2432 2464 2432 Size: 32 5363 AlwNI CAG_nnn'CTG Cuts at: 2387 2431 3138 4710 2387 Size: 44 707 1572 3072 Fragments arranged by size: 3072 1572 707 44 ApaLI G'TGCA_C Cuts at: 3452 4608 3452 Size: 1156 4239 ApoI r'AATT_y Cuts at: 1493 2704 2930 3082 5191 1493 Size: 1211 226 152 2109 1697 Fragments arranged by size: 2109 1697 1211 226 152 AseI AT'TA_AT Cuts at: 160 1032 3949 160 Size: 872 2917 1606 Fragments arranged by size: 2917 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5395 AvaI C'yCGr_G Cuts at: 1870 1943 2273 2603 2720 1870 Size: 73 330 330 117 4545 Fragments arranged by size: 4545 330 330 117 73 BamHI G'GATC_C Cuts at: 2725 3092 2725 Size: 367 5028 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2138 2222 2300 2475 Size: 325 217 627 345 6 84 78 175 Cuts at: 2475 2609 2716 4171 618 Size: 134 107 1455 1842 Fragments arranged by size: 1842 1455 627 345 325 217 175 134 107 84 78 6 BanII G_rGCy'C Cuts at: 1391 2198 2280 2714 1391 Size: 807 82 434 4072 Fragments arranged by size: 4072 807 434 82 BbeI G_GCGC'C Cuts at: 2136 2142 2226 2304 2479 2613 2136 Size: 6 84 78 175 134 4918 Fragments arranged by size: 4918 175 134 84 78 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2370 2688 928 Size: 971 471 318 3635 Fragments arranged by size: 3635 971 471 318 BbvCI CC'TCA_GC Cuts at: 2343 2343 Size: 5395 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2272 2306 2473 2507 3586 3620 1903 Size: 34 335 34 167 34 1079 34 3678 Fragments arranged by size: 3678 1079 335 167 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3287 4497 3287 Size: 1210 4185 BclI T'GATC_A Cuts at: 1548 1548 Size: 5395 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4006 136 Size: 107 122 71 631 605 2334 1525 Fragments arranged by size: 2334 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2529 5390 2529 Size: 2861 2534 BlpI GC'TnA_GC Cuts at: 2812 2812 Size: 5395 Bme1580I G_kGCm'C Cuts at: 1790 2619 3456 4612 1790 Size: 829 837 1156 2573 Fragments arranged by size: 2573 1156 837 829 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5395 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3126 4084 442 Size: 416 53 83 399 24 1709 958 1753 Fragments arranged by size: 1753 1709 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5395 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2459 4055 1239 Size: 1214 6 1596 2579 Fragments arranged by size: 2579 1596 1214 6 Bpu10I CC'TnA_GC Cuts at: 2343 2343 Size: 5395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3520 4385 4683 3520 Size: 865 298 4232 Fragments arranged by size: 4232 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4058 882 Size: 617 2559 2219 Fragments arranged by size: 2559 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3186 493 Size: 711 1982 2702 Fragments arranged by size: 2702 1982 711 BsaBI GATnn'nnATC Cuts at: 2528 5389 2528 Size: 2861 2534 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2139 2223 2229 Size: 53 83 186 936 600 6 84 6 Cuts at: 2229 2301 2476 2610 3202 3584 4219 275 Size: 72 175 134 592 382 635 1451 Fragments arranged by size: 1451 936 635 600 592 382 186 175 134 84 83 72 53 6 6 BsaWI w'CCGG_w Cuts at: 3828 4226 4500 4647 5058 3828 Size: 398 274 147 411 4165 Fragments arranged by size: 4165 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2432 2462 1178 Size: 30 165 30 1029 30 4111 Fragments arranged by size: 4111 1029 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2672 2702 1186 Size: 1486 30 3879 Fragments arranged by size: 3879 1486 30 BseYI C'CCAG_C Cuts at: 4598 5094 4598 Size: 496 4899 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4822 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2154 2845 3606 4634 1540 Size: 275 186 153 691 761 1028 2301 Fragments arranged by size: 2301 1028 761 691 275 186 153 BsiHKAI G_wGCw'C Cuts at: 3456 3541 4612 3456 Size: 85 1071 4239 Fragments arranged by size: 4239 1071 85 BsmI GAATG_Cn' Cuts at: 2906 2999 2906 Size: 93 5302 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2280 2619 2714 3456 3541 4612 Size: 399 408 82 339 95 742 85 1071 Cuts at: 4612 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 408 399 339 95 85 82 BspEI T'CCGG_A Cuts at: 5058 5058 Size: 5395 BspHI T'CATG_A Cuts at: 3284 5014 3284 Size: 1730 3665 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2250 2750 844 Size: 1406 500 3489 Fragments arranged by size: 3489 1406 500 BsrBI CCG'CTC Cuts at: 1394 1823 3282 1394 Size: 429 1459 3507 Fragments arranged by size: 3507 1459 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3890 4064 65 Size: 1183 2642 174 1396 Fragments arranged by size: 2642 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2135 4039 4226 1700 Size: 110 325 1904 187 2869 Fragments arranged by size: 2869 1904 325 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5395 BssHII G'CGCG_C Cuts at: 1925 2325 1925 Size: 400 4995 BssSI C'ACGA_G Cuts at: 3455 4467 3455 Size: 1012 4383 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5395 BstBI TT'CG_AA Cuts at: 4199 4199 Size: 5395 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5395 BstYI r'GATC_y Cuts at: 1972 2529 2725 3092 3477 3494 4946 5032 5044 Size: 557 196 367 385 17 1452 86 12 Cuts at: 5044 5390 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 557 385 367 346 196 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2640 513 Size: 552 1575 3268 Fragments arranged by size: 3268 1575 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4656 Fragments arranged by size: 4656 683 56 BtsI GCAGTG_nn' Cuts at: 2290 2631 2911 3705 3725 2290 Size: 341 280 794 20 3960 Fragments arranged by size: 3960 794 341 280 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5395 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5360 DraI TTT'AAA Cuts at: 2699 2699 Size: 5395 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4402 809 Size: 507 3086 1802 Fragments arranged by size: 3086 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2842 3731 5360 Size: 54 1425 50 275 18 1012 889 1629 Cuts at: 5360 8 Size: 43 Fragments arranged by size: 1629 1425 1012 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2842 1537 Size: 275 1030 4090 Fragments arranged by size: 4090 1030 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2670 3325 1353 Size: 1317 655 3423 Fragments arranged by size: 3423 1317 655 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3982 4356 4502 387 Size: 1064 138 2393 374 146 1280 Fragments arranged by size: 2393 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2364 2453 2459 3458 4055 4842 5110 Size: 393 732 89 6 999 597 787 268 Cuts at: 5110 1239 Size: 1524 Fragments arranged by size: 1524 999 787 732 597 393 268 89 6 EcoO109I rG'GnC_Cy Cuts at: 2384 2384 Size: 5395 EcoRI G'AATT_C Cuts at: 2704 3082 2704 Size: 378 5017 FspI TGC'GCA Cuts at: 3901 3901 Size: 5395 HaeII r_GCGC'y Cuts at: 1678 2136 2142 2226 2304 2479 2521 2613 4542 Size: 458 6 84 78 175 42 92 1929 Cuts at: 4542 1678 Size: 2531 Fragments arranged by size: 2531 1929 458 175 92 84 78 42 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4036 4068 4110 4142 1373 Size: 32 135 32 2464 32 42 32 2626 Fragments arranged by size: 2626 2464 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2739 2985 669 Size: 988 281 238 563 246 3079 Fragments arranged by size: 3079 988 563 281 246 238 HindIII A'AGCT_T Cuts at: 748 748 Size: 5395 HpaI GTT'AAC Cuts at: 2985 2985 Size: 5395 KasI G'GCGC_C Cuts at: 2132 2138 2222 2300 2475 2609 2132 Size: 6 84 78 175 134 4918 Fragments arranged by size: 4918 175 134 84 78 6 KpnI G_GTAC'C Cuts at: 2720 2720 Size: 5395 MfeI C'AATT_G Cuts at: 2994 2994 Size: 5395 MluI A'CGCG_T Cuts at: 5271 5271 Size: 5395 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2609 4509 4693 1305 Size: 1304 1900 184 2007 Fragments arranged by size: 2007 1900 1304 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3573 Fragments arranged by size: 3573 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3353 3712 3871 518 Size: 648 2187 359 159 2042 Fragments arranged by size: 2187 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2642 2811 3488 4636 4881 1606 Size: 1036 169 677 1148 245 2120 Fragments arranged by size: 2120 1148 1036 677 245 169 NaeI GCC'GGC Cuts at: 2137 2137 Size: 5395 NarI GG'CG_CC Cuts at: 2133 2139 2223 2301 2476 2610 2133 Size: 6 84 78 175 134 4918 Fragments arranged by size: 4918 175 134 84 78 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4843 NdeI CA'TA_TG Cuts at: 387 387 Size: 5395 NgoMIV G'CCGG_C Cuts at: 2135 2135 Size: 5395 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5395 NotI GC'GGCC_GC Cuts at: 2842 2842 Size: 5395 NspI r_CATG'y Cuts at: 1132 1229 2753 4298 1132 Size: 97 1524 1545 2229 Fragments arranged by size: 2229 1545 1524 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5395 PciI A'CATG_T Cuts at: 4294 4294 Size: 5395 PflMI CCAn_nnn'nTGG Cuts at: 1108 2431 1108 Size: 1323 4072 PmeI GTTT'AAAC Cuts at: 2699 2699 Size: 5395 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2432 2464 3464 3496 4977 5009 2432 Size: 32 1000 32 1481 32 2818 Fragments arranged by size: 2818 1481 1000 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2384 2384 Size: 5395 PshAI GACnn'nnGTC Cuts at: 1613 2580 1613 Size: 967 4428 PsiI TTA'TAA Cuts at: 2965 2965 Size: 5395 PstI C_TGCA'G Cuts at: 830 2285 2747 830 Size: 1455 462 3478 Fragments arranged by size: 3478 1455 462 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5395 SacII CC_GC'GG Cuts at: 2643 2643 Size: 5395 SalI G'TCGA_C Cuts at: 1936 2737 1936 Size: 801 4594 SbfI CC_TGCA'GG Cuts at: 2747 2747 Size: 5395 ScaI AGT'ACT Cuts at: 3643 5355 3643 Size: 1712 3683 SfcI C'TryA_G Cuts at: 826 1045 2281 2743 3878 4559 4750 826 Size: 219 1236 462 1135 681 191 1471 Fragments arranged by size: 1471 1236 1135 681 462 219 191 SfoI GGC'GCC Cuts at: 2134 2140 2224 2302 2477 2611 2134 Size: 6 84 78 175 134 4918 Fragments arranged by size: 4918 175 134 84 78 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5395 SgrAI Cr'CCGG_yG Cuts at: 1700 2135 1700 Size: 435 4960 SmaI CCC'GGG Cuts at: 1872 2275 2722 1872 Size: 403 447 4545 Fragments arranged by size: 4545 447 403 SmlI C'TyrA_G Cuts at: 820 1017 1943 3499 4400 4662 820 Size: 197 926 1556 901 262 1553 Fragments arranged by size: 1556 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3186 493 Size: 2693 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5395 SphI G_CATG'C Cuts at: 1132 2753 1132 Size: 1621 3774 SspI AAT'ATT Cuts at: 5 52 3319 5 Size: 47 3267 2081 Fragments arranged by size: 3267 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5395 StyI C'CwwG_G Cuts at: 513 1065 2834 513 Size: 552 1769 3074 Fragments arranged by size: 3074 1769 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3422 3439 3592 3777 4222 325 Size: 1255 1842 17 153 185 445 1498 Fragments arranged by size: 1842 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3641 5353 96 Size: 274 80 33 51 1443 1664 1712 138 Fragments arranged by size: 1712 1664 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5355 5387 5355 Size: 32 5363 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5395 XbaI T'CTAG_A Cuts at: 2731 2731 Size: 5395 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5395 XmaI C'CCGG_G Cuts at: 1870 2273 2720 1870 Size: 403 447 4545 Fragments arranged by size: 4545 447 403 XmnI GAAnn'nnTTC Cuts at: 2447 3524 2447 Size: 1077 4318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3203 276 Size: 53 83 186 936 1669 2468 Fragments arranged by size: 2468 1669 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI