(Circular) (Six-base) MAPSORT of: pFN21AB9689.seq Check: 9613 from: 1 to: 5395 pFN21AB9689 5395 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3205 278 Size: 53 83 186 936 1669 2468 Fragments arranged by size: 2468 1669 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2738 1937 Size: 801 4594 Acc65I G'GTAC_C Cuts at: 2716 2716 Size: 5395 AclI AA'CG_TT Cuts at: 1563 3522 3895 1563 Size: 1959 373 3063 Fragments arranged by size: 3063 1959 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3458 4842 5110 1632 Size: 1826 1384 268 1917 Fragments arranged by size: 1917 1826 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5198 AflIII A'CryG_T Cuts at: 1203 4294 5271 1203 Size: 3091 977 1327 Fragments arranged by size: 3091 1327 977 AgeI A'CCGG_T Cuts at: 4226 4226 Size: 5395 AhdI GACnn_n'nnGTC Cuts at: 4124 4124 Size: 5395 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5395 AlwNI CAG_nnn'CTG Cuts at: 3138 4710 3138 Size: 1572 3823 ApaLI G'TGCA_C Cuts at: 2510 3452 4608 2510 Size: 942 1156 3297 Fragments arranged by size: 3297 1156 942 ApoI r'AATT_y Cuts at: 1493 2704 2930 3082 5191 1493 Size: 1211 226 152 2109 1697 Fragments arranged by size: 2109 1697 1211 226 152 AscI GG'CGCG_CC Cuts at: 2583 2583 Size: 5395 AseI AT'TA_AT Cuts at: 160 1032 3949 160 Size: 872 2917 1606 Fragments arranged by size: 2917 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5395 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2133 2720 1870 Size: 73 70 120 587 4545 Fragments arranged by size: 4545 587 120 73 70 BamHI G'GATC_C Cuts at: 2725 3092 2725 Size: 367 5028 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2590 2644 2716 4171 Size: 325 217 627 286 517 54 72 1455 Cuts at: 4171 618 Size: 1842 Fragments arranged by size: 1842 1455 627 517 325 286 217 72 54 BanII G_rGCy'C Cuts at: 1391 2226 2714 1391 Size: 835 488 4072 Fragments arranged by size: 4072 835 488 BbeI G_GCGC'C Cuts at: 2077 2594 2648 2077 Size: 517 54 4824 Fragments arranged by size: 4824 517 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4424 BbvCI CC'TCA_GC Cuts at: 2108 2108 Size: 5395 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3586 3620 1903 Size: 34 1649 34 3678 Fragments arranged by size: 3678 1649 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3287 4497 3287 Size: 1210 4185 BclI T'GATC_A Cuts at: 1548 1548 Size: 5395 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2237 2354 2474 Size: 107 122 71 631 605 565 117 120 Cuts at: 2474 4006 136 Size: 1532 1525 Fragments arranged by size: 1532 1525 631 605 565 122 120 117 107 71 BglII A'GATC_T Cuts at: 5390 5390 Size: 5395 BlpI GC'TnA_GC Cuts at: 2812 2812 Size: 5395 Bme1580I G_kGCm'C Cuts at: 1790 2514 3456 4612 1790 Size: 724 942 1156 2573 Fragments arranged by size: 2573 1156 942 724 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5395 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2255 2501 3126 Size: 416 53 83 399 24 838 246 625 Cuts at: 3126 4084 442 Size: 958 1753 Fragments arranged by size: 1753 958 838 625 416 399 246 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5395 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2183 2226 2424 2701 4055 1239 Size: 905 39 43 198 277 1354 2579 Fragments arranged by size: 2579 1354 905 277 198 43 39 Bpu10I CC'TnA_GC Cuts at: 2108 2108 Size: 5395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2036 2221 3520 4385 4683 2036 Size: 185 1299 865 298 2748 Fragments arranged by size: 2748 1299 865 298 185 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2677 4058 882 Size: 617 1178 1381 2219 Fragments arranged by size: 2219 1381 1178 617 BsaAI yAC'GTr Cuts at: 493 1204 3186 493 Size: 711 1982 2702 Fragments arranged by size: 2702 1982 711 BsaBI GATnn'nnATC Cuts at: 5389 5389 Size: 5395 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2074 2271 2346 2591 Size: 53 83 186 936 541 197 75 245 Cuts at: 2591 2645 3202 3584 4219 275 Size: 54 557 382 635 1451 Fragments arranged by size: 1451 936 635 557 541 382 245 197 186 83 75 54 53 BsaWI w'CCGG_w Cuts at: 2148 2300 2600 3828 4226 4500 4647 5058 2148 Size: 152 300 1228 398 274 147 411 2485 Fragments arranged by size: 2485 1228 411 398 300 274 152 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5170 Fragments arranged by size: 5170 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5395 BseYI C'CCAG_C Cuts at: 2367 4598 5094 2367 Size: 2231 496 2668 Fragments arranged by size: 2668 2231 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 2477 1163 Size: 573 633 108 4081 Fragments arranged by size: 4081 633 573 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2232 2358 2845 3606 4634 1540 Size: 275 186 231 126 487 761 1028 2301 Fragments arranged by size: 2301 1028 761 487 275 231 186 126 BsiHKAI G_wGCw'C Cuts at: 2514 3456 3541 4612 2514 Size: 942 85 1071 3297 Fragments arranged by size: 3297 1071 942 85 BsmI GAATG_Cn' Cuts at: 2906 2999 2906 Size: 93 5302 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2514 2714 3456 3541 4612 1391 Size: 399 436 288 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 288 200 85 BspEI T'CCGG_A Cuts at: 2148 5058 2148 Size: 2910 2485 BspHI T'CATG_A Cuts at: 3284 5014 3284 Size: 1730 3665 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2750 844 Size: 1906 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2128 3282 1394 Size: 429 305 1154 3507 Fragments arranged by size: 3507 1154 429 305 BsrDI GCAATG_nn' Cuts at: 65 1248 3890 4064 65 Size: 1183 2642 174 1396 Fragments arranged by size: 2642 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2422 2473 2587 4039 4226 1700 Size: 110 612 51 114 1452 187 2869 Fragments arranged by size: 2869 1452 612 187 114 110 51 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5395 BssHII G'CGCG_C Cuts at: 1925 2566 2583 1925 Size: 641 17 4737 Fragments arranged by size: 4737 641 17 BssSI C'ACGA_G Cuts at: 3455 4467 3455 Size: 1012 4383 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5395 BstBI TT'CG_AA Cuts at: 4199 4199 Size: 5395 BstEII G'GTnAC_C Cuts at: 1184 2008 1184 Size: 824 4571 BstYI r'GATC_y Cuts at: 1972 2428 2725 3092 3477 3494 4946 5032 5044 Size: 456 297 367 385 17 1452 86 12 Cuts at: 5044 5390 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 456 385 367 346 297 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 2235 513 Size: 552 939 186 45 3673 Fragments arranged by size: 3673 939 552 186 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4656 Fragments arranged by size: 4656 683 56 BtsI GCAGTG_nn' Cuts at: 2911 3705 3725 2911 Size: 794 20 4581 Fragments arranged by size: 4581 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5395 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5360 DraI TTT'AAA Cuts at: 2699 2699 Size: 5395 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4402 809 Size: 507 3086 1802 Fragments arranged by size: 3086 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2355 2842 Size: 54 1425 50 275 18 399 126 487 Cuts at: 2842 3731 5360 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 487 399 275 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2229 2355 2842 1537 Size: 275 417 126 487 4090 Fragments arranged by size: 4090 487 417 275 126 EarI CTCTTCn'nnn_ Cuts at: 1353 2097 2378 3325 1353 Size: 744 281 947 3423 Fragments arranged by size: 3423 947 744 281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3982 4356 4502 387 Size: 1064 138 2393 374 146 1280 Fragments arranged by size: 2393 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2183 2226 2424 2701 3458 4055 Size: 393 512 39 43 198 277 757 597 Cuts at: 4055 4842 5110 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 757 597 512 393 277 268 198 43 39 EcoO109I rG'GnC_Cy Cuts at: 2653 2653 Size: 5395 EcoRI G'AATT_C Cuts at: 2704 3082 2704 Size: 378 5017 FspI TGC'GCA Cuts at: 2539 3901 2539 Size: 1362 4033 HaeII r_GCGC'y Cuts at: 1678 2077 2282 2447 2503 2594 2648 4542 1678 Size: 399 205 165 56 91 54 1894 2531 Fragments arranged by size: 2531 1894 399 205 165 91 56 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4036 4068 4110 4142 1373 Size: 32 135 32 2464 32 42 32 2626 Fragments arranged by size: 2626 2464 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2739 2985 669 Size: 988 281 801 246 3079 Fragments arranged by size: 3079 988 801 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5395 HpaI GTT'AAC Cuts at: 2985 2985 Size: 5395 KasI G'GCGC_C Cuts at: 2073 2590 2644 2073 Size: 517 54 4824 Fragments arranged by size: 4824 517 54 KpnI G_GTAC'C Cuts at: 2720 2720 Size: 5395 MfeI C'AATT_G Cuts at: 2994 2994 Size: 5395 MluI A'CGCG_T Cuts at: 5271 5271 Size: 5395 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4509 4693 1305 Size: 3204 184 2007 Fragments arranged by size: 3204 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3573 Fragments arranged by size: 3573 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3353 3712 3871 518 Size: 648 2187 359 159 2042 Fragments arranged by size: 2187 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2237 2289 2659 2811 3488 4636 4881 1606 Size: 631 52 370 152 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 631 370 245 152 52 NaeI GCC'GGC Cuts at: 2589 2589 Size: 5395 NarI GG'CG_CC Cuts at: 2074 2591 2645 2074 Size: 517 54 4824 Fragments arranged by size: 4824 517 54 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3904 Fragments arranged by size: 3904 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5395 NgoMIV G'CCGG_C Cuts at: 2587 2587 Size: 5395 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5395 NotI GC'GGCC_GC Cuts at: 2842 2842 Size: 5395 NspI r_CATG'y Cuts at: 1132 1229 2753 4298 1132 Size: 97 1524 1545 2229 Fragments arranged by size: 2229 1545 1524 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5395 PciI A'CATG_T Cuts at: 4294 4294 Size: 5395 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5395 PfoI T'CCnGG_A Cuts at: 2265 2265 Size: 5395 PmeI GTTT'AAAC Cuts at: 2699 2699 Size: 5395 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3464 3496 4977 5009 3464 Size: 32 1481 32 3850 Fragments arranged by size: 3850 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5395 PsiI TTA'TAA Cuts at: 2965 2965 Size: 5395 PstI C_TGCA'G Cuts at: 830 2747 830 Size: 1917 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5395 PvuII CAG'CTG Cuts at: 2289 2289 Size: 5395 SacII CC_GC'GG Cuts at: 2238 2238 Size: 5395 SalI G'TCGA_C Cuts at: 1936 2737 1936 Size: 801 4594 SapI GCTCTTCn'nnn_ Cuts at: 2097 2378 2097 Size: 281 5114 SbfI CC_TGCA'GG Cuts at: 2747 2747 Size: 5395 ScaI AGT'ACT Cuts at: 3643 5355 3643 Size: 1712 3683 SfcI C'TryA_G Cuts at: 826 1045 2743 3878 4559 4750 826 Size: 219 1698 1135 681 191 1471 Fragments arranged by size: 1698 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2075 2592 2646 2075 Size: 517 54 4824 Fragments arranged by size: 4824 517 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5395 SgrAI Cr'CCGG_yG Cuts at: 1700 2587 1700 Size: 887 4508 SmaI CCC'GGG Cuts at: 1872 2015 2135 2722 1872 Size: 143 120 587 4545 Fragments arranged by size: 4545 587 143 120 SmlI C'TyrA_G Cuts at: 820 1017 1943 2051 2200 3499 4400 4662 820 Size: 197 926 108 149 1299 901 262 1553 Fragments arranged by size: 1553 1299 926 901 262 197 149 108 SnaBI TAC'GTA Cuts at: 493 3186 493 Size: 2693 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5395 SphI G_CATG'C Cuts at: 1132 2753 1132 Size: 1621 3774 SspI AAT'ATT Cuts at: 5 52 3319 5 Size: 47 3267 2081 Fragments arranged by size: 3267 2081 47 StuI AGG'CCT Cuts at: 1517 2437 1517 Size: 920 4475 StyI C'CwwG_G Cuts at: 513 1065 2004 2479 2649 2687 2834 513 Size: 552 939 475 170 38 147 3074 Fragments arranged by size: 3074 939 552 475 170 147 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3422 3439 3592 3777 4222 325 Size: 1255 1842 17 153 185 445 1498 Fragments arranged by size: 1842 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3641 5353 96 Size: 274 80 33 51 1443 1664 1712 138 Fragments arranged by size: 1712 1664 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5355 5387 5355 Size: 32 5363 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5395 XbaI T'CTAG_A Cuts at: 2731 2731 Size: 5395 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5395 XmaI C'CCGG_G Cuts at: 1870 2013 2133 2720 1870 Size: 143 120 587 4545 Fragments arranged by size: 4545 587 143 120 XmnI GAAnn'nnTTC Cuts at: 3524 3524 Size: 5395 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3203 276 Size: 53 83 186 936 1669 2468 Fragments arranged by size: 2468 1669 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI