Restriction map for
pFN21AB9678
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HapII
HgaI Hin1I HindIII HinfI HpaI
HpaII KasI KpnI KspI MaeI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 33 GCGG CCGC(-3/-1)
BanII 226 GGGCTC G[GA]GC[CT]^C
BbvCI 72 GCTGAGG CCTCAGC(-5/-2)
BglII 11 AGATCT A^GATCT
BlpI 111 GCTGAGC GC^T[ACGT]AGC
BmyI 226 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC
BsaJI 26 CCAGGG C^C[ACGT][ACGT]GG
Bsp1286I 226 GGGCTC G[AGT]GC[ACT]^C
BspMI 248 ACCTGC ACCTGC(4/8)
BsrBI 31 GAGCGG CCGCTC(-3/-3)
BsrDI 139 GCAATG GCAATG(2/0)
BstAPI 180 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 84 CATCC GGATG(2/0)
BstYI 11 AGATCT [GA]^GATC[CT]
Cac8I 185 GCCTGC GC[ACGT]^[ACGT]GC
CelII 111 GCTGAGC GC^T[ACGT]AGC
CfoI 59 GCGC GCG^C
DpnII 12 GATC ^GATC
DraII 240 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 229 CTCTTC CTCTTC(1/4)
EcoO109I 240 AGGACCC [GA]G^G[ACGT]CC[CT]
FokI 84 CATCC GGATG(9/13)
HaeII 58 AGCGCC [GA]GCGC^[CT]
HaeIII 256 GGCC GG^CC
HhaI 59 GCGC GCG^C
HinP1I 59 GCGC G^CGC
HincII 52 GTCAAC GT[CT]^[GA]AC
HindII 52 GTCAAC GT[CT]^[GA]AC
Ksp632I 229 CTCTTC CTCTTC(1/4)
MboI 12 GATC ^GATC
MflI 11 AGATCT [GA]^GATC[CT]
NdeII 12 GATC ^GATC
PpuMI 240 AGGACCC [GA]G^G[AT]CC[CT]
SapI 228 GCTCTTC GCTCTTC(1/4)
Sau3AI 12 GATC ^GATC
StuI 255 AGGCCT AGG^CCT
XhoII 11 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Asp700I 9 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
124 GAAACGCTTC
AvaII 241 GGACC G^G[AT]CC
304 GGTCC
BbvI 42 GCAGC GCAGC(8/12)
205 GCTGC
BpmI 98 CTCCAG CTGGAG(16/14)
259 CTGGAG
Cfr13I 241 GGACC G^G[ACGT]CC
304 GGTCC
Fnu4HI 42 GCAGC GC^[ACGT]GC
205 GCTGC
HphI 93 TCACC GGTGA(8/7)
298 TCACC
ItaI 42 GCAGC GC^[ACGT]GC
205 GCTGC
NlaIV 241 GGACCC GG[ACGT]^[ACGT]CC
291 GGTTCC
Sau96I 241 GGACC G^G[ACGT]CC
304 GGTCC
SexAI 287 ACCAGGT A^CC[AT]GGT
300 ACCTGGT
SmlI 236 CTCAAG C^T[CT][GA]AG
277 CTTGAG
TseI 42 GCAGC G^C[AT]GC
205 GCTGC
XmnI 9 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
124 GAAACGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AluI BsiYI BslI BssKI BstNI
DdeI EcoRII MboII MnlI MvaI
MwoI ScrFI