(Circular) (Six-base) MAPSORT of: pFN21AB9671.seq Check: 5296 from: 1 to: 6253 pFN21AB9671 6253 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4063 278 Size: 53 83 186 936 2527 2468 Fragments arranged by size: 2527 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3596 1937 Size: 1659 4594 Acc65I G'GTAC_C Cuts at: 3574 3574 Size: 6253 AclI AA'CG_TT Cuts at: 1563 4380 4753 1563 Size: 2817 373 3063 Fragments arranged by size: 3063 2817 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2881 2965 3049 3217 4316 5700 5968 Size: 492 757 84 84 168 1099 1384 268 Cuts at: 5968 1632 Size: 1917 Fragments arranged by size: 1917 1384 1099 757 492 268 168 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6056 AflIII A'CryG_T Cuts at: 1203 3545 5152 6129 1203 Size: 2342 1607 977 1327 Fragments arranged by size: 2342 1607 1327 977 AgeI A'CCGG_T Cuts at: 5084 5084 Size: 6253 AhdI GACnn_n'nnGTC Cuts at: 4982 4982 Size: 6253 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6253 AlwNI CAG_nnn'CTG Cuts at: 3996 5568 3996 Size: 1572 4681 ApaLI G'TGCA_C Cuts at: 4310 5466 4310 Size: 1156 5097 ApoI r'AATT_y Cuts at: 1493 2427 2568 2595 2652 2931 3157 3241 3267 Size: 934 141 27 57 279 226 84 26 Cuts at: 3267 3351 3562 3788 3940 6049 1493 Size: 84 211 226 152 2109 1697 Fragments arranged by size: 2109 1697 934 279 226 226 211 152 141 84 84 57 27 26 AseI AT'TA_AT Cuts at: 160 1032 4807 160 Size: 872 3775 1606 Fragments arranged by size: 3775 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 AvaI C'yCGr_G Cuts at: 1870 1943 3578 1870 Size: 73 1635 4545 Fragments arranged by size: 4545 1635 73 BamHI G'GATC_C Cuts at: 3583 3950 3583 Size: 367 5886 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3574 5029 618 Size: 325 217 627 1787 1455 1842 Fragments arranged by size: 1842 1787 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3572 1391 Size: 2181 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2467 2635 2803 2887 2971 3055 3223 Size: 971 568 168 168 84 84 84 168 Cuts at: 3223 3307 928 Size: 84 3874 Fragments arranged by size: 3874 971 568 168 168 168 84 84 84 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4444 4478 1903 Size: 34 50 34 2423 34 3678 Fragments arranged by size: 3678 2423 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4145 5355 4145 Size: 1210 5043 BclI T'GATC_A Cuts at: 1548 2125 2760 1548 Size: 577 635 5041 Fragments arranged by size: 5041 635 577 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 6253 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4864 136 Size: 107 122 71 631 605 3192 1525 Fragments arranged by size: 3192 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2388 6248 2388 Size: 3860 2393 BlpI GC'TnA_GC Cuts at: 3670 3670 Size: 6253 Bme1580I G_kGCm'C Cuts at: 1790 4314 5470 1790 Size: 2524 1156 2573 Fragments arranged by size: 2573 2524 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6253 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3984 4942 442 Size: 416 53 83 399 24 2567 958 1753 Fragments arranged by size: 2567 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2457 2541 2709 2793 2877 2947 2961 3031 Size: 1218 84 168 84 84 70 14 70 Cuts at: 3031 3045 3213 3297 3381 3465 4913 1239 Size: 14 168 84 84 84 1448 2579 Fragments arranged by size: 2579 1448 1218 168 168 84 84 84 84 84 84 70 70 14 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4378 5243 5541 4378 Size: 865 298 5090 Fragments arranged by size: 5090 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4916 882 Size: 617 3417 2219 Fragments arranged by size: 3417 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4044 493 Size: 711 2840 2702 Fragments arranged by size: 2840 2702 711 BsaBI GATnn'nnATC Cuts at: 6247 6247 Size: 6253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4060 4442 5077 275 Size: 53 83 186 936 2527 382 635 1451 Fragments arranged by size: 2527 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2603 4686 5084 5358 5505 5916 2603 Size: 2083 398 274 147 411 2940 Fragments arranged by size: 2940 2083 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6028 Fragments arranged by size: 6028 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6253 BseYI C'CCAG_C Cuts at: 5456 5952 5456 Size: 496 5757 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5680 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3703 4464 5492 1540 Size: 275 186 1702 761 1028 2301 Fragments arranged by size: 2301 1702 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4314 4399 5470 4314 Size: 85 1071 5097 Fragments arranged by size: 5097 1071 85 BsmI GAATG_Cn' Cuts at: 3146 3764 3857 3146 Size: 618 93 5542 Fragments arranged by size: 5542 618 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2351 2351 Size: 6253 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3572 4314 4399 5470 1391 Size: 399 1782 742 85 1071 2174 Fragments arranged by size: 2174 1782 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5916 5916 Size: 6253 BspHI T'CATG_A Cuts at: 4142 5872 4142 Size: 1730 4523 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3608 844 Size: 2764 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4140 1394 Size: 429 2317 3507 Fragments arranged by size: 3507 2317 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4748 4922 65 Size: 1183 3500 174 1396 Fragments arranged by size: 3500 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3107 4897 5084 1700 Size: 110 1297 1790 187 2869 Fragments arranged by size: 2869 1790 1297 187 110 BsrGI T'GTAC_A Cuts at: 96 2749 3169 96 Size: 2653 420 3180 Fragments arranged by size: 3180 2653 420 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6253 BssSI C'ACGA_G Cuts at: 4313 5325 4313 Size: 1012 5241 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6253 BstBI TT'CG_AA Cuts at: 5057 5057 Size: 6253 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6253 BstYI r'GATC_y Cuts at: 1972 2388 3583 3950 4335 4352 5804 5890 5902 Size: 416 1195 367 385 17 1452 86 12 Cuts at: 5902 6248 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1195 416 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5701 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2489 487 Size: 683 56 1263 4251 Fragments arranged by size: 4251 1263 683 56 BtsI GCAGTG_nn' Cuts at: 3769 4563 4583 3769 Size: 794 20 5439 Fragments arranged by size: 5439 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6253 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6218 DraI TTT'AAA Cuts at: 2368 3557 2368 Size: 1189 5064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5260 809 Size: 507 3944 1802 Fragments arranged by size: 3944 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3700 4589 6218 Size: 54 1425 50 275 18 1870 889 1629 Cuts at: 6218 8 Size: 43 Fragments arranged by size: 1870 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3700 1537 Size: 275 1888 4090 Fragments arranged by size: 4090 1888 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3488 4183 1353 Size: 2135 695 3423 Fragments arranged by size: 3423 2135 695 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4840 5214 5360 387 Size: 1064 138 3251 374 146 1280 Fragments arranged by size: 3251 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2457 2541 2709 2793 2877 2881 Size: 393 492 333 84 168 84 84 4 Cuts at: 2881 2947 2961 2965 3031 3045 3049 3213 3217 Size: 66 14 4 66 14 4 164 4 Cuts at: 3217 3297 3381 3465 4316 4913 5700 5968 1239 Size: 80 84 84 851 597 787 268 1524 Fragments arranged by size: 1524 851 787 597 492 393 333 268 168 164 84 84 84 84 84 80 66 66 14 14 4 4 4 4 EcoRI G'AATT_C Cuts at: 2427 2595 2931 3267 3562 3940 2427 Size: 168 336 336 295 378 4740 Fragments arranged by size: 4740 378 336 336 295 168 FspI TGC'GCA Cuts at: 4759 4759 Size: 6253 HaeII r_GCGC'y Cuts at: 1678 5400 1678 Size: 3722 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4894 4926 4968 5000 1373 Size: 32 135 32 3322 32 42 32 2626 Fragments arranged by size: 3322 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 3597 3843 669 Size: 988 281 208 1451 246 3079 Fragments arranged by size: 3079 1451 988 281 246 208 HindIII A'AGCT_T Cuts at: 748 3285 3396 748 Size: 2537 111 3605 Fragments arranged by size: 3605 2537 111 HpaI GTT'AAC Cuts at: 3843 3843 Size: 6253 KpnI G_GTAC'C Cuts at: 3578 3578 Size: 6253 MfeI C'AATT_G Cuts at: 3852 3852 Size: 6253 MluI A'CGCG_T Cuts at: 6129 6129 Size: 6253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5367 5551 1305 Size: 4062 184 2007 Fragments arranged by size: 4062 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4431 Fragments arranged by size: 4431 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2214 2218 2452 2536 3108 4211 4570 Size: 648 1048 4 234 84 572 1103 359 Cuts at: 4570 4729 518 Size: 159 2042 Fragments arranged by size: 2042 1103 1048 648 572 359 234 159 84 4 MspA1I CmG'CkG Cuts at: 1606 3669 4346 5494 5739 1606 Size: 2063 677 1148 245 2120 Fragments arranged by size: 2120 2063 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5701 NdeI CA'TA_TG Cuts at: 387 2215 387 Size: 1828 4425 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6253 NotI GC'GGCC_GC Cuts at: 3700 3700 Size: 6253 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 6253 NspI r_CATG'y Cuts at: 1132 1229 3549 3611 5156 1132 Size: 97 2320 62 1545 2229 Fragments arranged by size: 2320 2229 1545 97 62 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6253 PciI A'CATG_T Cuts at: 3545 5152 3545 Size: 1607 4646 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6253 PmeI GTTT'AAAC Cuts at: 3557 3557 Size: 6253 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4322 4354 5835 5867 4322 Size: 32 1481 32 4708 Fragments arranged by size: 4708 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6253 PsiI TTA'TAA Cuts at: 2619 3123 3291 3823 2619 Size: 504 168 532 5049 Fragments arranged by size: 5049 532 504 168 PstI C_TGCA'G Cuts at: 830 2091 3605 830 Size: 1261 1514 3478 Fragments arranged by size: 3478 1514 1261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6253 SalI G'TCGA_C Cuts at: 1936 3595 1936 Size: 1659 4594 SbfI CC_TGCA'GG Cuts at: 3605 3605 Size: 6253 ScaI AGT'ACT Cuts at: 4501 6213 4501 Size: 1712 4541 SfcI C'TryA_G Cuts at: 826 1045 2087 3601 4736 5417 5608 826 Size: 219 1042 1514 1135 681 191 1471 Fragments arranged by size: 1514 1471 1135 1042 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6253 SmaI CCC'GGG Cuts at: 1872 3580 1872 Size: 1708 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4357 5258 5520 820 Size: 197 926 2414 901 262 1553 Fragments arranged by size: 2414 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4044 493 Size: 3551 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6253 SphI G_CATG'C Cuts at: 1132 3611 1132 Size: 2479 3774 SspI AAT'ATT Cuts at: 5 52 2266 4177 5 Size: 47 2214 1911 2081 Fragments arranged by size: 2214 2081 1911 47 StuI AGG'CCT Cuts at: 1517 2306 1517 Size: 789 5464 StyI C'CwwG_G Cuts at: 513 1065 3692 513 Size: 552 2627 3074 Fragments arranged by size: 3074 2627 552 SwaI ATTT'AAAT Cuts at: 2368 2368 Size: 6253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3308 4280 4297 4450 4635 5080 325 Size: 1255 1728 972 17 153 185 445 1498 Fragments arranged by size: 1728 1498 1255 972 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2749 3169 4499 Size: 274 80 33 51 1443 772 420 1330 Cuts at: 4499 6211 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1330 772 420 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2894 2926 2978 3010 6213 6245 2894 Size: 32 52 32 3203 32 2902 Fragments arranged by size: 3203 2902 52 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6253 XbaI T'CTAG_A Cuts at: 3589 3589 Size: 6253 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6253 XmaI C'CCGG_G Cuts at: 1870 3578 1870 Size: 1708 4545 XmnI GAAnn'nnTTC Cuts at: 3314 3371 3455 4382 3314 Size: 57 84 927 5185 Fragments arranged by size: 5185 927 84 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4061 276 Size: 53 83 186 936 2527 2468 Fragments arranged by size: 2527 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI