(Circular) (Six-base) MAPSORT of: pFN21AB9664.seq Check: 3782 from: 1 to: 6079 pFN21AB9664 6079 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2287 3889 278 Size: 53 83 186 936 751 1602 2468 Fragments arranged by size: 2468 1602 936 751 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 2635 2971 3055 3307 4142 5526 5794 Size: 588 415 336 84 252 835 1384 268 Cuts at: 5794 1632 Size: 1917 Fragments arranged by size: 1917 1384 835 588 415 336 268 252 84 AfeI AGC'GCT Cuts at: 2996 2996 Size: 6079 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 4978 5955 1203 Size: 3775 977 1327 Fragments arranged by size: 3775 1327 977 AgeI A'CCGG_T Cuts at: 3099 4910 3099 Size: 1811 4268 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 3822 5394 3822 Size: 1572 4507 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 3388 3614 3766 5875 1493 Size: 1895 226 152 2109 1697 Fragments arranged by size: 2109 1895 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 2746 3404 1870 Size: 73 803 658 4545 Fragments arranged by size: 4545 803 658 73 BamHI G'GATC_C Cuts at: 2446 3409 3776 2446 Size: 963 367 4749 Fragments arranged by size: 4749 963 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3400 4855 618 Size: 325 217 627 1613 1455 1842 Fragments arranged by size: 1842 1613 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2747 2831 2915 3083 3167 3335 3398 1391 Size: 1356 84 84 168 84 168 63 4072 Fragments arranged by size: 4072 1356 168 168 84 84 84 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4270 4304 1903 Size: 34 2333 34 3678 Fragments arranged by size: 3678 2333 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2424 4690 136 Size: 107 122 71 631 605 752 2266 1525 Fragments arranged by size: 2266 1525 752 631 605 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 4140 5296 1790 Size: 2350 1156 2573 Fragments arranged by size: 2573 2350 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3810 4768 442 Size: 416 53 83 399 24 2393 958 1753 Fragments arranged by size: 2393 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2967 3219 3303 4739 1239 Size: 1728 252 84 1436 2579 Fragments arranged by size: 2579 1728 1436 252 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2194 2815 4204 5069 5367 2194 Size: 621 1389 865 298 2906 Fragments arranged by size: 2906 1389 865 621 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2679 4742 882 Size: 617 1180 2063 2219 Fragments arranged by size: 2219 2063 1180 617 BsaAI yAC'GTr Cuts at: 493 1204 3870 493 Size: 711 2666 2702 Fragments arranged by size: 2702 2666 711 BsaBI GATnn'nnATC Cuts at: 2485 2586 6073 2485 Size: 101 3487 2491 Fragments arranged by size: 3487 2491 101 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2284 3886 4268 4903 Size: 53 83 186 936 751 1602 382 635 Cuts at: 4903 275 Size: 1451 Fragments arranged by size: 1602 1451 936 751 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3099 4512 4910 5184 5331 5742 3099 Size: 1413 398 274 147 411 3436 Fragments arranged by size: 3436 1413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2734 2764 2818 2848 1178 Size: 30 165 30 1331 30 54 30 4409 Fragments arranged by size: 4409 1331 165 54 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 2984 3248 1186 Size: 1357 441 264 4017 Fragments arranged by size: 4017 1357 441 264 BseYI C'CCAG_C Cuts at: 5282 5778 5282 Size: 496 5583 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2906 2990 3322 1163 Size: 573 1170 84 332 3920 Fragments arranged by size: 3920 1170 573 332 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3529 4290 5318 1540 Size: 275 186 1528 761 1028 2301 Fragments arranged by size: 2301 1528 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2747 2831 2915 3083 3167 3335 4140 4225 5296 Size: 84 84 168 84 168 805 85 1071 Cuts at: 5296 2747 Size: 3530 Fragments arranged by size: 3530 1071 805 168 168 85 84 84 84 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 BsmBI CGTCTCn'nnnn_ Cuts at: 2291 2291 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2747 2831 2915 3083 3167 3335 3398 Size: 399 957 84 84 168 84 168 63 Cuts at: 3398 4140 4225 5296 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 957 742 399 168 168 85 84 84 84 63 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3012 3434 844 Size: 2168 422 3489 Fragments arranged by size: 3489 2168 422 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2726 4574 4748 65 Size: 1183 1478 1848 174 1396 Fragments arranged by size: 1848 1478 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3099 4723 4910 1700 Size: 110 1289 1624 187 2869 Fragments arranged by size: 2869 1624 1289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 2169 4883 2169 Size: 2714 3365 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2446 3169 3370 3409 3776 4161 4178 5630 Size: 474 723 201 39 367 385 17 1452 Cuts at: 5630 5716 5728 6074 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 723 474 385 367 346 201 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2262 2262 Size: 6079 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 2607 3595 4389 4409 2607 Size: 988 794 20 4277 Fragments arranged by size: 4277 988 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2425 3526 Size: 54 1425 50 275 18 177 418 1101 Cuts at: 3526 4415 6044 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1101 889 418 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3526 1537 Size: 275 1714 4090 Fragments arranged by size: 4090 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 2239 4009 1353 Size: 804 82 1770 3423 Fragments arranged by size: 3423 1770 804 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2745 2829 2913 3081 3165 3333 2745 Size: 84 84 168 84 168 5491 Fragments arranged by size: 5491 168 168 84 84 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 2635 2967 2971 3055 3219 3303 Size: 393 588 415 332 4 84 164 84 Cuts at: 3303 3307 4142 4739 5526 5794 1239 Size: 4 835 597 787 268 1524 Fragments arranged by size: 1524 835 787 597 588 415 393 332 268 164 84 84 4 4 EcoO109I rG'GnC_Cy Cuts at: 3039 3039 Size: 6079 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 2998 5226 1678 Size: 1320 2228 2531 Fragments arranged by size: 2531 2228 1320 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2734 2766 2818 2850 2974 Size: 32 135 32 1162 32 52 32 124 Cuts at: 2974 3006 3154 3186 4720 4752 4794 4826 1373 Size: 32 148 32 1534 32 42 32 2626 Fragments arranged by size: 2626 1534 1162 148 135 124 52 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2096 3423 3669 669 Size: 988 281 158 1327 246 3079 Fragments arranged by size: 3079 1327 988 281 246 158 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3360 5193 5377 1305 Size: 2055 1833 184 2007 Fragments arranged by size: 2055 2007 1833 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2427 10 Size: 54 1425 343 177 418 3662 Fragments arranged by size: 3662 1425 418 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2592 2778 4037 4396 4555 518 Size: 648 1426 186 1259 359 159 2042 Fragments arranged by size: 2042 1426 1259 648 359 186 159 MspA1I CmG'CkG Cuts at: 1606 3355 3495 4172 5320 5565 1606 Size: 1749 140 677 1148 245 2120 Fragments arranged by size: 2120 1749 1148 677 245 140 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 2593 387 Size: 2206 3873 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 3437 4982 1132 Size: 97 2208 1545 2229 Fragments arranged by size: 2229 2208 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 2793 2877 3649 2793 Size: 84 772 5223 Fragments arranged by size: 5223 772 84 PspXI vC'TCGA_Gb Cuts at: 2746 2746 Size: 6079 PstI C_TGCA'G Cuts at: 830 2614 3431 830 Size: 1784 817 3478 Fragments arranged by size: 3478 1784 817 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 SacI G_AGCT'C Cuts at: 2747 2831 2915 3083 3167 3335 2747 Size: 84 84 168 84 168 5491 Fragments arranged by size: 5491 168 168 84 84 84 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SfcI C'TryA_G Cuts at: 826 1045 2610 3427 4562 5243 5434 826 Size: 219 1565 817 1135 681 191 1471 Fragments arranged by size: 1565 1471 1135 817 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 3099 1700 Size: 1399 4680 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2209 2746 2830 4183 5084 5346 Size: 197 926 266 537 84 1353 901 262 Cuts at: 5346 820 Size: 1553 Fragments arranged by size: 1553 1353 926 901 537 266 262 197 84 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2004 3518 513 Size: 552 939 1514 3074 Fragments arranged by size: 3074 1514 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2012 3022 3274 Size: 274 80 33 51 1443 35 1010 252 Cuts at: 3274 4325 6037 96 Size: 1051 1712 138 Fragments arranged by size: 1712 1443 1051 1010 274 252 138 80 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6039 6071 6039 Size: 32 6047 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2552 2552 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 2746 1943 Size: 803 5276 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 2732 2816 3068 3152 3320 4208 2732 Size: 84 252 84 168 888 4603 Fragments arranged by size: 4603 888 252 168 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2285 3887 276 Size: 53 83 186 936 751 1602 2468 Fragments arranged by size: 2468 1602 936 751 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI