Restriction map for
pFN21AB9652
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BspDI BspEI BspMI BsrDI BsrI
BssHII BssSI Bst1107I BstBI BstF5I
BstXI BstYI BstZ17I Bsu36I ClaI
DraI DraIII DrdI Eam1105I Eco81I
EcoNI EcoRI EcoRV EcoT22I FokI
FseI HincII HindII HindIII HinfI
HpaI HphI KpnI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tsp45I Tsp509I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AflIII 878 ACATGT A^C[GA][CT]GT
Aor51HI 294 AGCGCT AGC^GCT
ApaI 36 GGGCCC GGGCC^C
Asp700I 135 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 310 GAGCAC G[AT]GC[AT]^C
AspI 115 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 549 CCCGGG C^[CT]CG[GA]G
AviII 561 TGCGCA TGC^GCA
BanII 36 GGGCCC G[GA]GC[CT]^C
BclI 818 TGATCA T^GATCA
BglI 381 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 280 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 280 GCTAAGC GC^T[ACGT]AGC
BsgI 298 CTGCAC GTGCAG(16/14)
BsiHKAI 310 GAGCAC G[AT]GC[AT]^C
BsmI 135 GAATGC GAATGC(1/-1)
BsoBI 549 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2 TGTACA T^GTACA
BspHI 356 TCATGA T^CATGA
BspLU11I 878 ACATGT A^CATGT
BsrGI 2 TGTACA T^GTACA
BstAPI 582 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 762 GGTTACC G^GT[ACGT]ACC
BstPI 762 GGTTACC G^GT[ACGT]ACC
CelII 280 GCTAAGC GC^T[ACGT]AGC
CpoI 113 CGGACCG CG^G[AT]CCG
DdeI 281 CTAAG C^T[ACGT]AG
DraII 803 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 768 CTCTTC CTCTTC(1/4)
Eco47III 294 AGCGCT AGC^GCT
EcoO109I 803 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 762 GGTTACC G^GT[ACGT]ACC
EcoT14I 716 CCATGG C^C[AT][AT]GG
FbaI 818 TGATCA T^GATCA
FspI 561 TGCGCA TGC^GCA
Ksp632I 768 CTCTTC CTCTTC(1/4)
MaeII 602 ACGT A^CGT
MseI 923 TTAA T^TAA
NcoI 716 CCATGG C^CATGG
PflFI 115 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 462 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 803 GGGACCT [GA]G^G[AT]CC[CT]
PstI 613 CTGCAG CTGCA^G
PvuII 529 CAGCTG CAG^CTG
RcaI 356 TCATGA T^CATGA
RsrII 113 CGGACCG CG^G[AT]CCG
SmaI 549 CCCGGG CCC^GGG
SspBI 2 TGTACA T^GTACA
StyI 716 CCATGG C^C[AT][AT]GG
TaqI 272 TCGA T^CGA
Tru9I 923 TTAA T^TAA
TspRI 932 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 115 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 272 TCGA T^CGA
Van91I 462 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 549 CCCGGG C^CCGGG
XmaI 549 CCCGGG C^CCGGG
XmnI 135 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BsiEI 86 CGGCCG CG[GA][CT]^CG
262 CGGCCG
BsmAI 16 GAGAC GTCTC(1/5)
250 GAGAC
BsmBI 16 GAGACG CGTCTC(1/5)
250 GAGACG
BsrBI 420 GAGCGG CCGCTC(-3/-3)
897 GAGCGG
DpnII 210 GATC ^GATC
819 GATC
EagI 86 CGGCCG C^GGCCG
262 CGGCCG
EclXI 86 CGGCCG C^GGCCG
262 CGGCCG
Eco52I 86 CGGCCG C^GGCCG
262 CGGCCG
HgaI 351 GACGC GACGC(5/10)
119 GCGTC
KspI 77 CCGCGG CCGC^GG
156 CCGCGG
MaeIII 476 GTTAC ^GT[ACGT]AC
763 GTTAC
MboI 210 GATC ^GATC
819 GATC
MboII 360 GAAGA GAAGA(8/7)
769 TCTTC
NaeI 88 GCCGGC GCC^GGC
439 GCCGGC
NdeII 210 GATC ^GATC
819 GATC
NgoMIV 88 GCCGGC G^CCGGC
439 GCCGGC
SacII 77 CCGCGG CCGC^GG
156 CCGCGG
Sau3AI 210 GATC ^GATC
819 GATC
XcmI 716 CCATGGGTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
749 CCAGCCCCCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BbeI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DsaI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI KasI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
NspI RsaI Sau96I ScrFI SfaNI
SfcI SfoI TseI