(Circular) (Six-base) MAPSORT of: pFN21AB9652.seq Check: 6441 from: 1 to: 5656 pFN21AB9652 5656 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 3719 5103 5371 1632 Size: 419 1668 1384 268 1917 Fragments arranged by size: 1917 1668 1384 419 268 AfeI AGC'GCT Cuts at: 2301 2301 Size: 5656 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 2883 4555 5532 1203 Size: 1680 1672 977 1327 Fragments arranged by size: 1680 1672 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 3399 4971 3399 Size: 1572 4084 ApaI G_GGCC'C Cuts at: 2045 2045 Size: 5656 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2554 2981 1870 Size: 73 611 427 4545 Fragments arranged by size: 4545 611 427 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2231 2441 2696 2791 2843 Size: 325 217 627 444 210 255 95 52 Cuts at: 2843 2851 2911 2977 4432 618 Size: 8 60 66 1455 1842 Fragments arranged by size: 1842 1455 627 444 325 255 217 210 95 66 60 52 8 BanII G_rGCy'C Cuts at: 1391 2045 2975 1391 Size: 654 930 4072 Fragments arranged by size: 4072 930 654 BbeI G_GCGC'C Cuts at: 2235 2445 2795 2847 2235 Size: 210 350 52 5044 Fragments arranged by size: 5044 350 210 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 2823 1548 Size: 1275 4381 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2392 4267 136 Size: 107 122 71 631 605 720 1875 1525 Fragments arranged by size: 1875 1525 720 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 2286 3073 2286 Size: 787 4869 Bme1580I G_kGCm'C Cuts at: 1790 2045 2699 2916 3717 4873 1790 Size: 255 654 217 801 1156 2573 Fragments arranged by size: 2573 1156 801 654 255 217 BmgBI CAC'GTC Cuts at: 1318 2608 1318 Size: 1290 4366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2767 2767 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2521 2737 2770 4316 1239 Size: 1282 216 33 1546 2579 Fragments arranged by size: 2579 1546 1282 216 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2442 2792 2844 Size: 53 83 186 936 699 210 350 52 Cuts at: 2844 3463 3845 4480 275 Size: 619 382 635 1451 Fragments arranged by size: 1451 936 699 635 619 382 350 210 186 83 53 52 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5431 Fragments arranged by size: 5431 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5656 BseYI C'CCAG_C Cuts at: 2536 2870 2889 4859 5355 2536 Size: 334 19 1970 496 2837 Fragments arranged by size: 2837 1970 496 334 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2288 1163 Size: 573 552 4531 Fragments arranged by size: 4531 573 552 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 2270 3106 3867 4895 1540 Size: 275 186 93 176 836 761 1028 2301 Fragments arranged by size: 2301 1028 836 761 275 186 176 93 BsiHKAI G_wGCw'C Cuts at: 2319 3717 3802 4873 2319 Size: 1398 85 1071 3102 Fragments arranged by size: 3102 1398 1071 85 BsmI GAATG_Cn' Cuts at: 2146 3167 3260 2146 Size: 1021 93 4542 Fragments arranged by size: 4542 1021 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2015 2249 2015 Size: 234 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2319 2699 2916 2975 3717 3802 Size: 399 255 274 380 217 59 742 85 Cuts at: 3802 4873 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 274 255 217 85 59 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 2361 3545 5275 2361 Size: 1184 1730 2742 Fragments arranged by size: 2742 1730 1184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3011 844 Size: 2167 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2427 2904 3543 1394 Size: 429 604 477 639 3507 Fragments arranged by size: 3507 639 604 477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 2334 2444 4300 4487 1700 Size: 110 283 241 110 1856 187 2869 Fragments arranged by size: 2869 1856 283 241 187 110 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 3745 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 2593 794 Size: 1799 3857 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 2767 1184 Size: 1583 4073 BstYI r'GATC_y Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651 Size: 1014 367 385 17 1452 86 12 346 Cuts at: 5651 1972 Size: 1977 Fragments arranged by size: 1977 1452 1014 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2082 2161 2721 513 Size: 552 1017 79 560 3448 Fragments arranged by size: 3448 1017 560 552 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 2936 3172 3966 3986 2936 Size: 236 794 20 4606 Fragments arranged by size: 4606 794 236 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2267 2922 Size: 54 1425 50 275 18 261 176 655 Cuts at: 2922 3103 3992 5621 8 Size: 181 889 1629 43 Fragments arranged by size: 1629 1425 889 655 275 261 181 176 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2091 2267 3103 1537 Size: 275 279 176 836 4090 Fragments arranged by size: 4090 836 279 275 176 EarI CTCTTCn'nnn_ Cuts at: 1353 2779 3586 1353 Size: 1426 807 3423 Fragments arranged by size: 3423 1426 807 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2359 4243 4617 4763 387 Size: 1064 138 770 1884 374 146 1280 Fragments arranged by size: 1884 1280 1064 770 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2521 2737 2770 3719 4316 5103 Size: 393 419 470 216 33 949 597 787 Cuts at: 5103 5371 1239 Size: 268 1524 Fragments arranged by size: 1524 949 787 597 470 419 393 268 216 33 EcoO109I rG'GnC_Cy Cuts at: 2809 2809 Size: 5656 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 2568 4162 2568 Size: 1594 4062 FspAI rTGC'GCAy Cuts at: 2568 2568 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 2227 2235 2303 2445 2616 2795 2847 4803 Size: 549 8 68 142 171 179 52 1956 Cuts at: 4803 1678 Size: 2531 Fragments arranged by size: 2531 1956 549 179 171 142 68 52 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373 Size: 32 135 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KasI G'GCGC_C Cuts at: 2231 2441 2791 2843 2231 Size: 210 350 52 5044 Fragments arranged by size: 5044 350 210 52 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 4770 4954 1305 Size: 927 2538 184 2007 Fragments arranged by size: 2538 2007 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3614 3973 4132 518 Size: 648 2448 359 159 2042 Fragments arranged by size: 2448 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2163 2170 2536 3072 3749 4897 5142 Size: 478 79 7 366 536 677 1148 245 Cuts at: 5142 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 536 478 366 245 79 7 NaeI GCC'GGC Cuts at: 2095 2446 2095 Size: 351 5305 NarI GG'CG_CC Cuts at: 2232 2442 2792 2844 2232 Size: 210 350 52 5044 Fragments arranged by size: 5044 350 210 52 NcoI C'CATG_G Cuts at: 513 1065 2721 513 Size: 552 1656 3448 Fragments arranged by size: 3448 1656 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NgoMIV G'CCGG_C Cuts at: 2093 2444 2093 Size: 351 5305 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2680 2833 2887 3014 4559 1132 Size: 97 1451 153 54 127 1545 2229 Fragments arranged by size: 2229 1545 1451 153 127 97 54 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 2883 4555 2883 Size: 1672 3984 PflMI CCAn_nnn'nTGG Cuts at: 1108 2473 1108 Size: 1365 4291 PfoI T'CCnGG_A Cuts at: 2814 2814 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2809 2809 Size: 5656 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 2328 3226 2328 Size: 898 4758 PspOMI G'GGCC_C Cuts at: 2041 2041 Size: 5656 PstI C_TGCA'G Cuts at: 830 2622 3008 830 Size: 1792 386 3478 Fragments arranged by size: 3478 1792 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2536 2536 Size: 5656 RsrII CG'GwC_CG Cuts at: 2119 2119 Size: 5656 SacII CC_GC'GG Cuts at: 2085 2164 2085 Size: 79 5577 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2515 2618 2671 3004 4139 4820 5011 Size: 219 1470 103 53 333 1135 681 191 Cuts at: 5011 826 Size: 1471 Fragments arranged by size: 1471 1470 1135 681 333 219 191 103 53 SfoI GGC'GCC Cuts at: 2233 2443 2793 2845 2233 Size: 210 350 52 5044 Fragments arranged by size: 5044 350 210 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2556 2983 1872 Size: 684 427 4545 Fragments arranged by size: 4545 684 427 SmlI C'TyrA_G Cuts at: 820 1017 1943 3760 4661 4923 820 Size: 197 926 1817 901 262 1553 Fragments arranged by size: 1817 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2721 3095 513 Size: 552 1656 374 3074 Fragments arranged by size: 3074 1656 552 374 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3700 3853 4038 4483 325 Size: 1255 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2376 2622 Size: 274 80 33 51 1443 30 369 246 Cuts at: 2622 3902 5614 96 Size: 1280 1712 138 Fragments arranged by size: 1712 1443 1280 369 274 246 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 2123 1316 Size: 807 4849 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2728 2761 2728 Size: 33 5623 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2554 2981 1870 Size: 684 427 4545 Fragments arranged by size: 4545 684 427 XmnI GAAnn'nnTTC Cuts at: 2144 3785 2144 Size: 1641 4015 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PmlI PspXI PsrI SacI SanDI SapI SexAI SfiI SrfI SwaI