Restriction map for
pFN21AB9652
(Linear) (Six-base) MAPSORT of: KIBB9652.seq Check: 327 from: 1 to: 951
KIBB9652.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 951
Size: 46 905
AfeI AGC'GCT
Cuts at: 0 296 951
Size: 296 655
AflIII A'CryG_T
Cuts at: 0 878 951
Size: 878 73
ApaI G_GGCC'C
Cuts at: 0 40 951
Size: 40 911
AvaI C'yCGr_G
Cuts at: 0 549 951
Size: 549 402
BanI G'GyrC_C
Cuts at: 0 226 436 691 786 838 846 906 951
Size: 226 210 255 95 52 8 60 45
Fragments arranged by size:
255 226 210 95 60 52 45 8
BanII G_rGCy'C
Cuts at: 0 40 951
Size: 40 911
BbeI G_GCGC'C
Cuts at: 0 230 440 790 842 951
Size: 230 210 350 52 109
Fragments arranged by size:
350 230 210 109 52
BclI T'GATC_A
Cuts at: 0 818 951
Size: 818 133
BglI GCCn_nnn'nGGC
Cuts at: 0 387 951
Size: 387 564
BlpI GC'TnA_GC
Cuts at: 0 281 951
Size: 281 670
Bme1580I G_kGCm'C
Cuts at: 0 40 694 911 951
Size: 40 654 217 40
Fragments arranged by size:
654 217 40 40
BmgBI CAC'GTC
Cuts at: 0 603 951
Size: 603 348
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 762 951
Size: 762 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 732 765 951
Size: 516 216 33 186
Fragments arranged by size:
516 216 186 33
BsaHI Gr'CG_yC
Cuts at: 0 227 437 787 839 951
Size: 227 210 350 52 112
Fragments arranged by size:
350 227 210 112 52
BseYI C'CCAG_C
Cuts at: 0 531 865 884 951
Size: 531 334 19 67
Fragments arranged by size:
531 334 67 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 951
Size: 283 668
BsiEI CG_ry'CG
Cuts at: 0 89 265 951
Size: 89 176 686
Fragments arranged by size:
686 176 89
BsiHKAI G_wGCw'C
Cuts at: 0 314 951
Size: 314 637
BsmI GAATG_Cn'
Cuts at: 0 141 951
Size: 141 810
BsmBI CGTCTCn'nnnn_
Cuts at: 0 10 244 951
Size: 10 234 707
Fragments arranged by size:
707 234 10
Bsp1286I G_dGCh'C
Cuts at: 0 40 314 694 911 951
Size: 40 274 380 217 40
Fragments arranged by size:
380 274 217 40 40
BspHI T'CATG_A
Cuts at: 0 356 951
Size: 356 595
BsrBI CCG'CTC
Cuts at: 0 422 899 951
Size: 422 477 52
Fragments arranged by size:
477 422 52
BsrFI r'CCGG_y
Cuts at: 0 88 329 439 951
Size: 88 241 110 512
Fragments arranged by size:
512 241 110 88
BsrGI T'GTAC_A
Cuts at: 0 2 951
Size: 2 949
BstAPI GCAn_nnn'nTGC
Cuts at: 0 588 951
Size: 588 363
BstEII G'GTnAC_C
Cuts at: 0 762 951
Size: 762 189
BtgI C'CryG_G
Cuts at: 0 77 156 716 951
Size: 77 79 560 235
Fragments arranged by size:
560 235 79 77
BtsI GCAGTG_nn'
Cuts at: 0 931 951
Size: 931 20
EaeI y'GGCC_r
Cuts at: 0 86 262 917 951
Size: 86 176 655 34
Fragments arranged by size:
655 176 86 34
EagI C'GGCC_G
Cuts at: 0 86 262 951
Size: 86 176 689
Fragments arranged by size:
689 176 86
EarI CTCTTCn'nnn_
Cuts at: 0 774 951
Size: 774 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 354 951
Size: 354 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 516 732 765 951
Size: 46 470 216 33 186
Fragments arranged by size:
470 216 186 46 33
EcoO109I rG'GnC_Cy
Cuts at: 0 804 951
Size: 804 147
FspI TGC'GCA
Cuts at: 0 563 951
Size: 563 388
FspAI rTGC'GCAy
Cuts at: 0 563 951
Size: 563 388
HaeII r_GCGC'y
Cuts at: 0 222 230 298 440 611 790 842 951
Size: 222 8 68 142 171 179 52 109
Fragments arranged by size:
222 179 171 142 109 68 52 8
KasI G'GCGC_C
Cuts at: 0 226 436 786 838 951
Size: 226 210 350 52 113
Fragments arranged by size:
350 226 210 113 52
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 951
Size: 227 724
MspA1I CmG'CkG
Cuts at: 0 79 158 165 531 951
Size: 79 79 7 366 420
Fragments arranged by size:
420 366 79 79 7
NaeI GCC'GGC
Cuts at: 0 90 441 951
Size: 90 351 510
Fragments arranged by size:
510 351 90
NarI GG'CG_CC
Cuts at: 0 227 437 787 839 951
Size: 227 210 350 52 112
Fragments arranged by size:
350 227 210 112 52
NcoI C'CATG_G
Cuts at: 0 716 951
Size: 716 235
NgoMIV G'CCGG_C
Cuts at: 0 88 439 951
Size: 88 351 512
Fragments arranged by size:
512 351 88
NspI r_CATG'y
Cuts at: 0 675 828 882 951
Size: 675 153 54 69
Fragments arranged by size:
675 153 69 54
PciI A'CATG_T
Cuts at: 0 878 951
Size: 878 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 468 951
Size: 468 483
PfoI T'CCnGG_A
Cuts at: 0 809 951
Size: 809 142
PpuMI rG'GwC_Cy
Cuts at: 0 804 951
Size: 804 147
PsiI TTA'TAA
Cuts at: 0 323 951
Size: 323 628
PspOMI G'GGCC_C
Cuts at: 0 36 951
Size: 36 915
PstI C_TGCA'G
Cuts at: 0 617 951
Size: 617 334
PvuII CAG'CTG
Cuts at: 0 531 951
Size: 531 420
RsrII CG'GwC_CG
Cuts at: 0 114 951
Size: 114 837
SacII CC_GC'GG
Cuts at: 0 80 159 951
Size: 80 79 792
Fragments arranged by size:
792 80 79
SfcI C'TryA_G
Cuts at: 0 510 613 666 951
Size: 510 103 53 285
Fragments arranged by size:
510 285 103 53
SfoI GGC'GCC
Cuts at: 0 228 438 788 840 951
Size: 228 210 350 52 111
Fragments arranged by size:
350 228 210 111 52
SmaI CCC'GGG
Cuts at: 0 551 951
Size: 551 400
StyI C'CwwG_G
Cuts at: 0 716 951
Size: 716 235
TatI w'GTAC_w
Cuts at: 0 2 371 617 951
Size: 2 369 246 334
Fragments arranged by size:
369 334 246 2
Tth111I GACn'n_nGTC
Cuts at: 0 118 951
Size: 118 833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 756 951
Size: 723 33 195
Fragments arranged by size:
723 195 33
XmaI C'CCGG_G
Cuts at: 0 549 951
Size: 549 402
XmnI GAAnn'nnTTC
Cuts at: 0 139 951
Size: 139 812
Enzymes that do cut:
AcuI AfeI AflIII ApaI AvaI BanI BanII BbeI
BclI BglI BlpI Bme1580I BmgBI BplI BpmI BsaHI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BsrBI BsrFI BsrGI BstAPI BstEII BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I FspI FspAI HaeII
KasI MmeI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PpuMI PsiI PspOMI PstI PvuII
RsrII SacII SfcI SfoI SmaI StyI TatI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BsiWI BspEI BspMI BsrDI
BssHII BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
EcoRV FalI FseI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI XbaI XhoI
ZraI