(Circular) (Six-base) MAPSORT of: pFN21AB9640.seq Check: 5011 from: 1 to: 5788 pFN21AB9640 5788 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2768 3851 5235 5503 1632 Size: 1136 1083 1384 268 1917 Fragments arranged by size: 1917 1384 1136 1083 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 2302 2400 2679 4687 5664 1203 Size: 1099 98 279 2008 977 1327 Fragments arranged by size: 2008 1327 1099 977 279 98 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2292 1184 Size: 1108 4680 AlwNI CAG_nnn'CTG Cuts at: 2488 3531 5103 2488 Size: 1043 1572 3173 Fragments arranged by size: 3173 1572 1043 ApaI G_GGCC'C Cuts at: 2201 2201 Size: 5788 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 3097 3323 3475 5584 1493 Size: 1604 226 152 2109 1697 Fragments arranged by size: 2109 1697 1604 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2162 2252 3113 1870 Size: 73 219 90 861 4545 Fragments arranged by size: 4545 861 219 90 73 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2359 3109 4564 618 Size: 325 217 627 572 750 1455 1842 Fragments arranged by size: 1842 1455 750 627 572 325 217 BanII G_rGCy'C Cuts at: 1391 2201 2511 2534 2801 3107 1391 Size: 810 310 23 267 306 4072 Fragments arranged by size: 4072 810 310 306 267 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2268 2998 928 Size: 971 369 730 3718 Fragments arranged by size: 3718 971 730 369 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2348 2357 4399 Size: 107 122 71 631 605 676 9 2042 Cuts at: 4399 136 Size: 1525 Fragments arranged by size: 2042 1525 676 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 2925 5783 2925 Size: 2858 2930 BlpI GC'TnA_GC Cuts at: 2723 3205 2723 Size: 482 5306 Bme1580I G_kGCm'C Cuts at: 1790 2201 2666 3849 5005 1790 Size: 411 465 1183 1156 2573 Fragments arranged by size: 2573 1183 1156 465 411 BmgBI CAC'GTC Cuts at: 1318 2245 2379 2647 1318 Size: 927 134 268 4459 Fragments arranged by size: 4459 927 268 134 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2182 2282 2537 Size: 416 53 83 399 24 765 100 255 Cuts at: 2537 3519 4477 442 Size: 982 958 1753 Fragments arranged by size: 1753 982 958 765 416 399 255 100 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2947 2947 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2448 2792 2903 2933 4448 1239 Size: 1209 344 111 30 1515 2579 Fragments arranged by size: 2579 1515 1209 344 111 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2882 3913 4778 5076 2882 Size: 1031 865 298 3594 Fragments arranged by size: 3594 1031 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2305 3579 493 Size: 711 1101 1274 2702 Fragments arranged by size: 2702 1274 1101 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2567 3595 3977 4612 Size: 53 83 186 936 1034 1028 382 635 Cuts at: 4612 275 Size: 1451 Fragments arranged by size: 1451 1034 1028 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2446 2476 1178 Size: 30 165 30 1043 30 4490 Fragments arranged by size: 4490 1043 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2576 2789 2920 1186 Size: 1390 213 131 4054 Fragments arranged by size: 4054 1390 213 131 BseYI C'CCAG_C Cuts at: 2144 2659 4991 5487 2144 Size: 515 2332 496 2445 Fragments arranged by size: 2445 2332 515 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2463 2474 2808 1163 Size: 573 727 11 334 4143 Fragments arranged by size: 4143 727 573 334 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2502 3849 3934 5005 2502 Size: 1347 85 1071 3285 Fragments arranged by size: 3285 1347 1071 85 BsmI GAATG_Cn' Cuts at: 2034 3299 3392 2034 Size: 1265 93 4430 Fragments arranged by size: 4430 1265 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2558 2879 2558 Size: 321 5467 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2502 2511 2534 2666 2801 3107 Size: 399 411 301 9 23 132 135 306 Cuts at: 3107 3849 3934 5005 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 306 301 135 132 85 23 9 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 2694 3143 844 Size: 1430 420 449 3489 Fragments arranged by size: 3489 1430 449 420 BsrBI CCG'CTC Cuts at: 1394 1823 2295 2430 3675 1394 Size: 429 472 135 1245 3507 Fragments arranged by size: 3507 1245 472 429 135 BsrDI GCAATG_nn' Cuts at: 65 1248 2155 2539 4283 4457 65 Size: 1183 907 384 1744 174 1396 Fragments arranged by size: 1744 1396 1183 907 384 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 2497 794 Size: 1703 4085 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 2176 1184 Size: 992 4796 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2230 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2925 3118 3485 3870 3887 5339 5425 5437 Size: 953 193 367 385 17 1452 86 12 Cuts at: 5437 5783 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 953 385 367 346 193 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2201 513 Size: 552 939 197 4100 Fragments arranged by size: 4100 939 552 197 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 3304 4098 4118 3304 Size: 794 20 4974 Fragments arranged by size: 4974 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DraIII CAC_nnn'GTG Cuts at: 2176 2497 3058 2176 Size: 321 561 4906 Fragments arranged by size: 4906 561 321 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2217 2386 Size: 54 1425 50 275 18 177 210 169 Cuts at: 2386 3235 4124 5753 8 Size: 849 889 1629 43 Fragments arranged by size: 1629 1425 889 849 275 210 177 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3718 1353 Size: 2365 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2795 3006 4375 4749 4895 387 Size: 1064 138 1206 211 1369 374 146 1280 Fragments arranged by size: 1369 1280 1206 1064 374 211 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2448 2768 2792 2903 2933 3851 4448 Size: 393 816 320 24 111 30 918 597 Cuts at: 4448 5235 5503 1239 Size: 787 268 1524 Fragments arranged by size: 1524 918 816 787 597 393 320 268 111 30 24 EcoO109I rG'GnC_Cy Cuts at: 2197 2197 Size: 5788 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2647 2647 Size: 5788 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 2660 4935 1678 Size: 982 2275 2531 Fragments arranged by size: 2531 2275 982 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2272 2304 4429 4461 4503 Size: 32 135 32 700 32 2125 32 42 Cuts at: 4503 4535 1373 Size: 32 2626 Fragments arranged by size: 2626 2125 700 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 2580 2934 4902 5086 1305 Size: 1260 15 354 1968 184 2007 Fragments arranged by size: 2007 1968 1260 354 184 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2219 10 Size: 54 1425 343 177 210 3579 Fragments arranged by size: 3579 1425 343 210 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 3746 4105 4264 518 Size: 648 1695 885 359 159 2042 Fragments arranged by size: 2042 1695 885 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2468 2722 3204 3881 5029 5274 1606 Size: 409 453 254 482 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 482 453 409 254 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2201 513 Size: 552 939 197 4100 Fragments arranged by size: 4100 939 552 197 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 2080 2437 3146 4691 1132 Size: 97 851 357 709 1545 2229 Fragments arranged by size: 2229 1545 851 709 357 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 2343 1108 Size: 1235 4553 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2197 2197 Size: 5788 PstI C_TGCA'G Cuts at: 830 2705 3140 830 Size: 1875 435 3478 Fragments arranged by size: 3478 1875 435 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2015 2468 2722 2015 Size: 453 254 5081 Fragments arranged by size: 5081 453 254 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2029 2701 3136 4271 4952 5143 826 Size: 219 984 672 435 1135 681 191 1471 Fragments arranged by size: 1471 1135 984 681 672 435 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 2254 3115 1872 Size: 382 861 4545 Fragments arranged by size: 4545 861 382 SmlI C'TyrA_G Cuts at: 820 1017 1943 2897 3892 4793 5055 820 Size: 197 926 954 995 901 262 1553 Fragments arranged by size: 1553 995 954 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 2080 2437 3146 1132 Size: 948 357 709 3774 Fragments arranged by size: 3774 948 709 357 SrfI GCCC'GGGC Cuts at: 2254 2254 Size: 5788 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 2714 1517 Size: 1197 4591 StyI C'CwwG_G Cuts at: 513 1065 2004 2201 3227 513 Size: 552 939 197 1026 3074 Fragments arranged by size: 3074 1026 939 552 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2164 2264 3815 3832 3985 4170 4615 Size: 1255 584 100 1551 17 153 185 445 Cuts at: 4615 325 Size: 1498 Fragments arranged by size: 1551 1498 1255 584 445 185 153 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2403 4034 5746 Size: 274 80 33 51 1443 426 1631 1712 Cuts at: 5746 96 Size: 138 Fragments arranged by size: 1712 1631 1443 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2446 2478 5748 5780 2446 Size: 32 3270 32 2454 Fragments arranged by size: 3270 2454 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2227 2227 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2252 3113 1870 Size: 382 861 4545 Fragments arranged by size: 4545 861 382 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI