(Circular) (Six-base) MAPSORT of: pFN21AB9621.seq Check: 6787 from: 1 to: 6757 pFN21AB9621 6757 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2025 2506 4567 278 Size: 53 83 186 936 489 481 2061 2468 Fragments arranged by size: 2468 2061 936 489 481 186 83 53 AccI GT'mk_AC Cuts at: 1937 2382 3612 4100 1937 Size: 445 1230 488 4594 Fragments arranged by size: 4594 1230 488 445 Acc65I G'GTAC_C Cuts at: 3800 4078 3800 Size: 278 6479 AclI AA'CG_TT Cuts at: 1563 4884 5257 1563 Size: 3321 373 3063 Fragments arranged by size: 3321 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2838 4820 6204 6472 1632 Size: 1206 1982 1384 268 1917 Fragments arranged by size: 1982 1917 1384 1206 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6560 AflIII A'CryG_T Cuts at: 1203 5656 6633 1203 Size: 4453 977 1327 Fragments arranged by size: 4453 1327 977 AgeI A'CCGG_T Cuts at: 5588 5588 Size: 6757 AhdI GACnn_n'nnGTC Cuts at: 2362 3967 5486 2362 Size: 1605 1519 3633 Fragments arranged by size: 3633 1605 1519 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6757 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3273 1184 Size: 2089 4668 AlwNI CAG_nnn'CTG Cuts at: 2434 2671 2743 3016 4500 6072 2434 Size: 237 72 273 1484 1572 3119 Fragments arranged by size: 3119 1572 1484 273 237 72 ApaI G_GGCC'C Cuts at: 2899 2899 Size: 6757 ApaLI G'TGCA_C Cuts at: 4814 5970 4814 Size: 1156 5601 ApoI r'AATT_y Cuts at: 1493 4066 4292 4444 6553 1493 Size: 2573 226 152 2109 1697 Fragments arranged by size: 2573 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3792 5311 160 Size: 872 2760 1519 1606 Fragments arranged by size: 2760 1606 1519 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 AvaI C'yCGr_G Cuts at: 1870 1943 2013 3091 4082 1870 Size: 73 70 1078 991 4545 Fragments arranged by size: 4545 1078 991 73 70 BamHI G'GATC_C Cuts at: 4087 4454 4087 Size: 367 6390 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2938 3800 4078 5533 618 Size: 325 217 627 1151 862 278 1455 1842 Fragments arranged by size: 1842 1455 1151 862 627 325 278 217 BanII G_rGCy'C Cuts at: 1391 2899 4076 1391 Size: 1508 1177 4072 Fragments arranged by size: 4072 1508 1177 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 2779 3556 928 Size: 971 126 754 777 4129 Fragments arranged by size: 4129 971 777 754 126 BbvCI CC'TCA_GC Cuts at: 3157 4023 3157 Size: 866 5891 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4948 4982 1903 Size: 34 50 34 2927 34 3678 Fragments arranged by size: 3678 2927 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4649 5859 4649 Size: 1210 5547 BclI T'GATC_A Cuts at: 1548 1548 Size: 6757 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2153 5368 136 Size: 107 122 71 631 605 481 3215 1525 Fragments arranged by size: 3215 1525 631 605 481 122 107 71 BglII A'GATC_T Cuts at: 2650 6752 2650 Size: 4102 2655 BlpI GC'TnA_GC Cuts at: 2612 3201 4174 2612 Size: 589 973 5195 Fragments arranged by size: 5195 973 589 Bme1580I G_kGCm'C Cuts at: 1790 2899 4818 5974 1790 Size: 1109 1919 1156 2573 Fragments arranged by size: 2573 1919 1156 1109 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6757 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2575 2766 3026 Size: 416 53 83 399 24 1158 191 260 Cuts at: 3026 3593 4488 5446 442 Size: 567 895 958 1753 Fragments arranged by size: 1753 1158 958 895 567 416 399 260 191 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2273 1056 Size: 1217 5540 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3979 3979 Size: 6757 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2765 3366 5417 1239 Size: 1526 601 2051 2579 Fragments arranged by size: 2579 2051 1526 601 Bpu10I CC'TnA_GC Cuts at: 3157 4023 3157 Size: 866 5891 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 2519 4882 5747 6045 2147 Size: 372 2363 865 298 2859 Fragments arranged by size: 2859 2363 865 372 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3327 3662 5420 882 Size: 617 1828 335 1758 2219 Fragments arranged by size: 2219 1828 1758 617 335 BsaAI yAC'GTr Cuts at: 493 1204 4548 493 Size: 711 3344 2702 Fragments arranged by size: 3344 2702 711 BsaBI GATnn'nnATC Cuts at: 3041 6751 3041 Size: 3710 3047 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 2216 2358 2503 Size: 53 83 186 936 489 194 142 145 Cuts at: 2503 2547 4564 4946 5581 275 Size: 44 2017 382 635 1451 Fragments arranged by size: 2017 1451 936 635 489 382 194 186 145 142 83 53 44 BsaWI w'CCGG_w Cuts at: 5190 5588 5862 6009 6420 5190 Size: 398 274 147 411 5527 Fragments arranged by size: 5527 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2429 2459 1178 Size: 30 165 30 1026 30 5476 Fragments arranged by size: 5476 1026 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2651 3145 1186 Size: 946 519 494 4798 Fragments arranged by size: 4798 946 519 494 BseYI C'CCAG_C Cuts at: 2664 3619 5960 6456 2664 Size: 955 2341 496 2965 Fragments arranged by size: 2965 2341 955 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2447 2506 2756 1163 Size: 573 711 59 250 5164 Fragments arranged by size: 5164 711 573 250 59 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4207 4968 5996 1540 Size: 275 186 2206 761 1028 2301 Fragments arranged by size: 2301 2206 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3853 4818 4903 5974 3853 Size: 965 85 1071 4636 Fragments arranged by size: 4636 1071 965 85 BsmI GAATG_Cn' Cuts at: 3374 4268 4361 3374 Size: 894 93 5770 Fragments arranged by size: 5770 894 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2899 3853 4076 4818 4903 5974 1391 Size: 399 1109 954 223 742 85 1071 2174 Fragments arranged by size: 2174 1109 1071 954 742 399 223 85 BspEI T'CCGG_A Cuts at: 6420 6420 Size: 6757 BspHI T'CATG_A Cuts at: 3603 3694 4646 6376 3603 Size: 91 952 1730 3984 Fragments arranged by size: 3984 1730 952 91 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4112 844 Size: 3268 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2713 2764 4644 1394 Size: 429 890 51 1880 3507 Fragments arranged by size: 3507 1880 890 429 51 BsrDI GCAATG_nn' Cuts at: 65 1248 5252 5426 65 Size: 1183 4004 174 1396 Fragments arranged by size: 4004 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5401 5588 1700 Size: 110 3591 187 2869 Fragments arranged by size: 3591 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2925 96 Size: 2829 3928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6757 BssSI C'ACGA_G Cuts at: 3852 4817 5829 3852 Size: 965 1012 4780 Fragments arranged by size: 4780 1012 965 BstAPI GCAn_nnn'nTGC Cuts at: 794 3489 794 Size: 2695 4062 BstBI TT'CG_AA Cuts at: 5561 5561 Size: 6757 BstEII G'GTnAC_C Cuts at: 1184 2915 1184 Size: 1731 5026 BstXI CCAn_nnnn'nTGG Cuts at: 2209 3037 2209 Size: 828 5929 BstYI r'GATC_y Cuts at: 1972 2650 3064 4087 4454 4839 4856 6308 6394 Size: 678 414 1023 367 385 17 1452 86 Cuts at: 6394 6406 6752 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1023 678 414 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2053 513 Size: 552 988 5217 Fragments arranged by size: 5217 988 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6018 Fragments arranged by size: 6018 683 56 BtsI GCAGTG_nn' Cuts at: 2744 4273 5067 5087 2744 Size: 1529 794 20 4414 Fragments arranged by size: 4414 1529 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6757 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6722 DraI TTT'AAA Cuts at: 4061 4061 Size: 6757 DraIII CAC_nnn'GTG Cuts at: 2353 3917 2353 Size: 1564 5193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3599 5764 809 Size: 507 2283 2165 1802 Fragments arranged by size: 2283 2165 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4204 5093 6722 Size: 54 1425 50 275 18 2374 889 1629 Cuts at: 6722 8 Size: 43 Fragments arranged by size: 2374 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4204 1537 Size: 275 2392 4090 Fragments arranged by size: 4090 2392 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4687 1353 Size: 3334 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3655 5344 5718 5864 387 Size: 1064 138 2066 1689 374 146 1280 Fragments arranged by size: 2066 1689 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2765 2838 3366 4820 5417 6204 6472 Size: 393 1133 73 528 1454 597 787 268 Cuts at: 6472 1239 Size: 1524 Fragments arranged by size: 1524 1454 1133 787 597 528 393 268 73 EcoO109I rG'GnC_Cy Cuts at: 2768 2895 2896 2768 Size: 127 1 6629 Fragments arranged by size: 6629 127 1 EcoRI G'AATT_C Cuts at: 4066 4444 4066 Size: 378 6379 FspI TGC'GCA Cuts at: 5263 5263 Size: 6757 HaeII r_GCGC'y Cuts at: 1678 2434 5904 1678 Size: 756 3470 2531 Fragments arranged by size: 3470 2531 756 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 3074 3106 3676 Size: 32 135 32 776 32 694 32 570 Cuts at: 3676 3708 3953 3985 5398 5430 5472 5504 1373 Size: 32 245 32 1413 32 42 32 2626 Fragments arranged by size: 2626 1413 776 694 570 245 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2383 3472 4101 4347 669 Size: 988 281 445 1089 629 246 3079 Fragments arranged by size: 3079 1089 988 629 445 281 246 HindIII A'AGCT_T Cuts at: 748 2564 748 Size: 1816 4941 HpaI GTT'AAC Cuts at: 4347 4347 Size: 6757 KpnI G_GTAC'C Cuts at: 3804 4082 3804 Size: 278 6479 MfeI C'AATT_G Cuts at: 4356 4356 Size: 6757 MluI A'CGCG_T Cuts at: 6633 6633 Size: 6757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2472 5871 6055 1305 Size: 1167 3399 184 2007 Fragments arranged by size: 3399 2007 1167 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4935 Fragments arranged by size: 4935 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 3290 4715 5074 5233 518 Size: 648 1041 1083 1425 359 159 2042 Fragments arranged by size: 2042 1425 1083 1041 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2226 2276 2461 2668 3161 4173 4850 5998 Size: 620 50 185 207 493 1012 677 1148 Cuts at: 5998 6243 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1012 677 620 493 245 207 185 50 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6205 NdeI CA'TA_TG Cuts at: 387 387 Size: 6757 NheI G'CTAG_C Cuts at: 1052 2269 1052 Size: 1217 5540 NotI GC'GGCC_GC Cuts at: 4204 4204 Size: 6757 NspI r_CATG'y Cuts at: 1132 1229 2302 2317 2455 3487 3561 4115 5660 Size: 97 1073 15 138 1032 74 554 1545 Cuts at: 5660 1132 Size: 2229 Fragments arranged by size: 2229 1545 1073 1032 554 138 97 74 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6757 PciI A'CATG_T Cuts at: 5656 5656 Size: 6757 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 3016 1108 Size: 1563 345 4849 Fragments arranged by size: 4849 1563 345 PmeI GTTT'AAAC Cuts at: 4061 4061 Size: 6757 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4826 4858 6339 6371 4826 Size: 32 1481 32 5212 Fragments arranged by size: 5212 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2768 2768 Size: 6757 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6757 PsiI TTA'TAA Cuts at: 4327 4327 Size: 6757 PspOMI G'GGCC_C Cuts at: 2895 2895 Size: 6757 PstI C_TGCA'G Cuts at: 830 2146 2782 2904 2995 4109 830 Size: 1316 636 122 91 1114 3478 Fragments arranged by size: 3478 1316 1114 636 122 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6757 PvuII CAG'CTG Cuts at: 2276 2461 2668 3161 2276 Size: 185 207 493 5872 Fragments arranged by size: 5872 493 207 185 SalI G'TCGA_C Cuts at: 1936 2381 4099 1936 Size: 445 1718 4594 Fragments arranged by size: 4594 1718 445 SanDI GG'GwC_CC Cuts at: 2768 2768 Size: 6757 SbfI CC_TGCA'GG Cuts at: 4109 4109 Size: 6757 ScaI AGT'ACT Cuts at: 2873 3928 5005 6717 2873 Size: 1055 1077 1712 2913 Fragments arranged by size: 2913 1712 1077 1055 SexAI A'CCwGG_T Cuts at: 3877 3877 Size: 6757 SfcI C'TryA_G Cuts at: 826 1045 2142 2456 2778 2900 2991 3373 4105 Size: 219 1097 314 322 122 91 382 732 Cuts at: 4105 5240 5921 6112 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1097 732 681 382 322 314 219 191 122 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6757 SmaI CCC'GGG Cuts at: 1872 2015 4084 1872 Size: 143 2069 4545 Fragments arranged by size: 4545 2069 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 2534 4861 5762 6024 820 Size: 197 926 219 372 2327 901 262 1553 Fragments arranged by size: 2327 1553 926 901 372 262 219 197 SnaBI TAC'GTA Cuts at: 493 4548 493 Size: 4055 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6757 SphI G_CATG'C Cuts at: 1132 3487 4115 1132 Size: 2355 628 3774 Fragments arranged by size: 3774 2355 628 SspI AAT'ATT Cuts at: 5 52 3652 4681 5 Size: 47 3600 1029 2081 Fragments arranged by size: 3600 2081 1029 47 StuI AGG'CCT Cuts at: 1517 3813 1517 Size: 2296 4461 StyI C'CwwG_G Cuts at: 513 1065 3030 4196 513 Size: 552 1965 1166 3074 Fragments arranged by size: 3074 1965 1166 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3211 3997 4784 4801 4954 5139 5584 Size: 1255 1631 786 787 17 153 185 445 Cuts at: 5584 325 Size: 1498 Fragments arranged by size: 1631 1498 1255 787 786 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2871 2925 3926 Size: 274 80 33 51 1443 894 54 1001 Cuts at: 3926 5003 6715 96 Size: 1077 1712 138 Fragments arranged by size: 1712 1443 1077 1001 894 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3770 3802 6717 6749 3770 Size: 32 2915 32 3778 Fragments arranged by size: 3778 2915 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6757 XbaI T'CTAG_A Cuts at: 4093 4093 Size: 6757 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 6757 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6757 XmaI C'CCGG_G Cuts at: 1870 2013 4082 1870 Size: 143 2069 4545 Fragments arranged by size: 4545 2069 143 XmnI GAAnn'nnTTC Cuts at: 2623 4886 2623 Size: 2263 4494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2023 2504 4565 276 Size: 53 83 186 936 489 481 2061 2468 Fragments arranged by size: 2468 2061 936 489 481 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI