Restriction map for
pFN21AB9616
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MscI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I RsaI RsrII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I TspRI Van91I XbaI
XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 276 TCCGGA T^CCGGA
AspI 33 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 405 GGTCC G^G[AT]CC
AviII 119 TGCGCA TGC^GCA
BglI 655 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 316 GAGACC GGTCTC(1/5)
BseAI 276 TCCGGA T^CCGGA
BspEI 276 TCCGGA T^CCGGA
BspHI 29 TCATGA T^CATGA
BsrFI 183 GCCGGC [GA]^CCGG[CT]
BsrI 402 ACTGG ACTGG(1/-1)
BssSI 646 CTCGTG CACGAG(-5/-1)
BstF5I 458 CATCC GGATG(2/0)
BstNI 519 CCTGG CC^[AT]GG
Cfr10I 183 GCCGGC [GA]^CCGG[CT]
DdeI 379 CTGAG C^T[ACGT]AG
DpnII 472 GATC ^GATC
DraII 158 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 442 GAAGAG CTCTTC(1/4)
EcoO109I 158 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 519 CCTGG ^CC[AT]GG
EcoT14I 149 CCAAGG C^C[AT][AT]GG
FokI 458 CATCC GGATG(9/13)
FspI 119 TGCGCA TGC^GCA
HphI 262 GGTGA GGTGA(8/7)
Ksp632I 442 GAAGAG CTCTTC(1/4)
KspI 569 CCGCGG CCGC^GG
MaeII 641 ACGT A^CGT
MboI 472 GATC ^GATC
MroI 276 TCCGGA T^CCGGA
MvaI 519 CCTGG CC^[AT]GG
NaeI 183 GCCGGC GCC^GGC
NdeII 472 GATC ^GATC
NgoMIV 183 GCCGGC G^CCGGC
PaeR7I 163 CTCGAG C^TCGAG
PflFI 33 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 471 CGATCG CGAT^CG
PvuII 103 CAGCTG CAG^CTG
RcaI 29 TCATGA T^CATGA
SacI 133 GAGCTC GAGCT^C
SacII 569 CCGCGG CCGC^GG
Sau3AI 472 GATC ^GATC
SfaNI 26 GCATC GCATC(5/9)
SfiI 654 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 128 CCCGGG CCC^GGG
SmlI 163 CTCGAG C^T[CT][GA]AG
StyI 149 CCAAGG C^C[AT][AT]GG
TaqI 164 TCGA T^CGA
Tth111I 33 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 164 TCGA T^CGA
XhoI 163 CTCGAG C^TCGAG
XmaCI 128 CCCGGG C^CCGGG
XmaI 128 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 104 AGCT AG^CT
134 AGCT
BanII 133 GAGCTC G[GA]GC[CT]^C
166 GAGCCC
BsaWI 92 ACCGGA [AT]^CCGG[AT]
276 TCCGGA
BseRI 313 GAGGAG GAGGAG(10/8)
445 GAGGAG
BsmAI 244 GAGAC GTCTC(1/5)
316 GAGAC
BsmFI 392 GGGAC GGGAC(10/14)
406 GTCCC
EagI 185 CGGCCG C^GGCCG
239 CGGCCG
EclXI 185 CGGCCG C^GGCCG
239 CGGCCG
Eco52I 185 CGGCCG C^GGCCG
239 CGGCCG
HinfI 96 GAGTC G^A[ACGT]TC
232 GAGTC
MspA1I 103 CAGCTG C[AC]G^C[GT]G
569 CCGCGG
NspI 65 GCATGC [GA]CATG^[CT]
632 GCATGC
PleI 96 GAGTC GAGTC(4/5)
232 GAGTC
SphI 65 GCATGC GCATG^C
632 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AspHI AvaI
BanI BbeI BbvI BcnI BmyI
BsaHI BsaJI BsgI BsiEI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BssKI
BstUI Cac8I CfoI Cfr13I DsaI
EaeI FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI KasI MboII MnlI
MspI MvnI MwoI NarI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI SfoI TseI