################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 1897 GTCGAC GT^[AC][GT]AC AflII 493 CTTAAG C^TTAAG AocI 352 CCTGAGG CC^T[ACGT]AGG BamHI 341 GGATCC G^GATCC BanII 1144 GGGCTC G[GA]GC[CT]^C BclI 1353 TGATCA T^GATCA BcnI 2067 CCCGG CC^[GC]GG BfrI 493 CTTAAG C^TTAAG BglI 1910 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1875 CTCCAG CTGGAG(16/14) BseRI 355 GAGGAG GAGGAG(10/8) BspLU11I 560 ACATGT A^CATGT BstAPI 770 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1244 TTCGAA TT^CGAA BstXI 1664 CCAAATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 352 CCTGAGG CC^T[ACGT]AGG DraII 2009 AGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 888 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 811 GACACTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1209 TGGCCG [CT]^GGCC[GA] Eco81I 352 CCTGAGG CC^T[ACGT]AGG EcoO109I 2009 AGGTCCC [GA]G^G[ACGT]CC[CT] FbaI 1353 TGATCA T^GATCA KspI 161 CCGCGG CCGC^GG NciI 2067 CCCGG CC^[GC]GG NcoI 1670 CCATGG C^CATGG NheI 1813 GCTAGC G^CTAGC NspV 1244 TTCGAA TT^CGAA PflMI 911 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2009 AGGTCCC [GA]G^G[AT]CC[CT] SacII 161 CCGCGG CCGC^GG SalI 1897 GTCGAC G^TCGAC ScaI 1563 AGTACT AGT^ACT SexAI 1002 ACCAGGT A^CC[AT]GGT SfuI 1244 TTCGAA TT^CGAA SmlI 493 CTTAAG C^T[CT][GA]AG SspI 618 AATATT AAT^ATT Van91I 911 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 459 CCAAAGAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 162 CGCG CG^CG 1937 CGCG AflIII 25 ACGTGT A^C[GA][CT]GT 560 ACATGT AseI 324 ATTAAT AT^TAAT 1449 ATTAAT AsnI 324 ATTAAT AT^TAAT 1449 ATTAAT Asp700I 259 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 608 GAACAGTTTC AvaII 433 GGTCC G^G[AT]CC 2010 GGTCC AvrII 822 CCTAGG C^CTAGG 1740 CCTAGG BbsI 1255 GTCTTC GAAGAC(2/6) 1471 GTCTTC BlnI 822 CCTAGG C^CTAGG 1740 CCTAGG BmyI 52 GGGCAC G[AGT]GC[ACT]^C 1144 GGGCTC BpuAI 1255 GTCTTC GAAGAC(2/6) 1471 GTCTTC BsaI 693 GAGACC GGTCTC(1/5) 1347 GGTCTC BsiYI 911 CCATTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2068 CCGGTTTCAGG BslI 911 CCATTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2068 CCGGTTTCAGG BsmFI 507 GGGAC GGGAC(10/14) 2011 GTCCC Bsp1286I 52 GGGCAC G[AGT]GC[ACT]^C 1144 GGGCTC BstUI 162 CGCG CG^CG 1937 CGCG BstYI 341 GGATCC [GA]^GATC[CT] 1539 AGATCC CfoI 544 GCGC GCG^C 1936 GCGC DraI 568 TTTAAA TTT^AAA 1519 TTTAAA DsaI 161 CCGCGG C^C[GA][CT]GG 1670 CCATGG HapII 1212 CCGG C^CGG 2068 CCGG HhaI 544 GCGC GCG^C 1936 GCGC HinP1I 544 GCGC G^CGC 1936 GCGC HincII 21 GTTGAC GT[CT]^[GA]AC 1897 GTCGAC HindII 21 GTTGAC GT[CT]^[GA]AC 1897 GTCGAC HpaII 1212 CCGG C^CGG 2068 CCGG MflI 341 GGATCC [GA]^GATC[CT] 1539 AGATCC MspA1I 161 CCGCGG C[AC]G^C[GT]G 1260 CAGCGG MspI 1212 CCGG C^CGG 2068 CCGG MvnI 162 CGCG CG^CG 1937 CGCG NspI 560 ACATGT [GA]CATG^[CT] 1495 ACATGC PleI 1880 GACTC GAGTC(4/5) 1253 GAGTC PshBI 324 ATTAAT AT^TAAT 1449 ATTAAT PstI 929 CTGCAG CTGCA^G 1712 CTGCAG XhoII 341 GGATCC [GA]^GATC[CT] 1539 AGATCC XmnI 259 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 608 GAACAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BfaI BsaJI BsmAI BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I