(Circular) (Six-base) MAPSORT of: pFN21AB9612.seq Check: 8909 from: 1 to: 6778 pFN21AB9612 6778 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4588 278 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4121 1937 Size: 2184 4594 Acc65I G'GTAC_C Cuts at: 4099 4099 Size: 6778 AclI AA'CG_TT Cuts at: 1563 4905 5278 1563 Size: 3342 373 3063 Fragments arranged by size: 3342 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 2720 3359 4841 6225 6493 1632 Size: 671 417 639 1482 1384 268 1917 Fragments arranged by size: 1917 1482 1384 671 639 417 268 AflII C'TTAA_G Cuts at: 820 1017 3032 820 Size: 197 2015 4566 Fragments arranged by size: 4566 2015 197 AflIII A'CryG_T Cuts at: 1203 5677 6654 1203 Size: 4474 977 1327 Fragments arranged by size: 4474 1327 977 AgeI A'CCGG_T Cuts at: 5609 5609 Size: 6778 AhdI GACnn_n'nnGTC Cuts at: 5507 5507 Size: 6778 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6778 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3622 1184 Size: 2438 4340 AlwNI CAG_nnn'CTG Cuts at: 4521 6093 4521 Size: 1572 5206 ApaLI G'TGCA_C Cuts at: 4835 5991 4835 Size: 1156 5622 ApoI r'AATT_y Cuts at: 1493 2967 3256 3350 3617 4087 4313 4465 6574 Size: 1474 289 94 267 470 226 152 2109 Cuts at: 6574 1493 Size: 1697 Fragments arranged by size: 2109 1697 1474 470 289 267 226 152 94 AseI AT'TA_AT Cuts at: 160 1032 5332 160 Size: 872 4300 1606 Fragments arranged by size: 4300 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 AvaI C'yCGr_G Cuts at: 1870 1943 4103 1870 Size: 73 2160 4545 Fragments arranged by size: 4545 2160 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3362 3395 3362 Size: 33 6745 BamHI G'GATC_C Cuts at: 4108 4475 4108 Size: 367 6411 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4099 5554 618 Size: 325 217 627 2312 1455 1842 Fragments arranged by size: 2312 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2743 3137 4097 1391 Size: 1352 394 960 4072 Fragments arranged by size: 4072 1352 960 394 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2291 3785 3862 928 Size: 971 392 1494 77 3844 Fragments arranged by size: 3844 1494 971 392 77 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4969 5003 1903 Size: 34 3032 34 3678 Fragments arranged by size: 3678 3032 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4670 5880 4670 Size: 1210 5568 BclI T'GATC_A Cuts at: 1548 1548 Size: 6778 BfrBI ATG'CAT Cuts at: 2486 3599 2486 Size: 1113 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5389 136 Size: 107 122 71 631 605 337 3380 1525 Fragments arranged by size: 3380 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6773 6773 Size: 6778 BlpI GC'TnA_GC Cuts at: 4195 4195 Size: 6778 Bme1580I G_kGCm'C Cuts at: 1790 4839 5995 1790 Size: 3049 1156 2573 Fragments arranged by size: 3049 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6778 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4509 5467 442 Size: 416 53 83 399 24 3092 958 1753 Fragments arranged by size: 3092 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6778 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3151 3151 Size: 6778 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2378 5438 1239 Size: 995 144 3060 2579 Fragments arranged by size: 3060 2579 995 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3121 4903 5768 6066 3121 Size: 1782 865 298 3833 Fragments arranged by size: 3833 1782 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3721 5441 882 Size: 617 2222 1720 2219 Fragments arranged by size: 2222 2219 1720 617 BsaAI yAC'GTr Cuts at: 493 1204 3410 4569 493 Size: 711 2206 1159 2702 Fragments arranged by size: 2702 2206 1159 711 BsaBI GATnn'nnATC Cuts at: 3913 6772 3913 Size: 2859 3919 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4585 4967 5602 275 Size: 53 83 186 936 3052 382 635 1451 Fragments arranged by size: 3052 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5211 5609 5883 6030 6441 5211 Size: 398 274 147 411 5548 Fragments arranged by size: 5548 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6553 Fragments arranged by size: 6553 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2855 3990 1186 Size: 1669 1135 3974 Fragments arranged by size: 3974 1669 1135 BseYI C'CCAG_C Cuts at: 5981 6477 5981 Size: 496 6282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6205 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4228 4989 6017 1540 Size: 275 186 2227 761 1028 2301 Fragments arranged by size: 2301 2227 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2438 3137 4839 4924 5995 2438 Size: 699 1702 85 1071 3221 Fragments arranged by size: 3221 1702 1071 699 85 BsmI GAATG_Cn' Cuts at: 3971 4289 4382 3971 Size: 318 93 6367 Fragments arranged by size: 6367 318 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2438 2743 3137 4097 4839 4924 5995 Size: 399 648 305 394 960 742 85 1071 Cuts at: 5995 1391 Size: 2174 Fragments arranged by size: 2174 1071 960 742 648 399 394 305 85 BspEI T'CCGG_A Cuts at: 6441 6441 Size: 6778 BspHI T'CATG_A Cuts at: 4667 6397 4667 Size: 1730 5048 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2967 4133 844 Size: 2123 1166 3489 Fragments arranged by size: 3489 2123 1166 BsrBI CCG'CTC Cuts at: 1394 1823 4665 1394 Size: 429 2842 3507 Fragments arranged by size: 3507 2842 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5273 5447 65 Size: 1183 4025 174 1396 Fragments arranged by size: 4025 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5422 5609 1700 Size: 110 3612 187 2869 Fragments arranged by size: 3612 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2489 96 Size: 2393 4385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6778 BssSI C'ACGA_G Cuts at: 4838 5850 4838 Size: 1012 5766 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6778 BstBI TT'CG_AA Cuts at: 5582 5582 Size: 6778 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6778 BstXI CCAn_nnnn'nTGG Cuts at: 3237 3237 Size: 6778 BstYI r'GATC_y Cuts at: 1972 4108 4475 4860 4877 6329 6415 6427 6773 Size: 2136 367 385 17 1452 86 12 346 Cuts at: 6773 1972 Size: 1977 Fragments arranged by size: 2136 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6039 Fragments arranged by size: 6039 683 56 BtsI GCAGTG_nn' Cuts at: 3892 4294 5088 5108 3892 Size: 402 794 20 5562 Fragments arranged by size: 5562 794 402 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6743 DraI TTT'AAA Cuts at: 2025 2248 2275 2569 3088 4033 4082 2025 Size: 223 27 294 519 945 49 4721 Fragments arranged by size: 4721 945 519 294 223 49 27 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5785 809 Size: 507 4469 1802 Fragments arranged by size: 4469 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3024 4225 5114 Size: 54 1425 50 275 18 1194 1201 889 Cuts at: 5114 6743 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1201 1194 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4225 1537 Size: 275 2413 4090 Fragments arranged by size: 4090 2413 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2750 4004 4708 1353 Size: 1397 1254 704 3423 Fragments arranged by size: 3423 1397 1254 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5365 5739 5885 387 Size: 1064 138 3776 374 146 1280 Fragments arranged by size: 3776 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3135 3135 Size: 6778 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2303 2378 2720 3359 4841 5438 Size: 393 602 69 75 342 639 1482 597 Cuts at: 5438 6225 6493 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1482 787 639 602 597 393 342 268 75 69 EcoO109I rG'GnC_Cy Cuts at: 3507 3507 Size: 6778 EcoRI G'AATT_C Cuts at: 3350 4087 4465 3350 Size: 737 378 5663 Fragments arranged by size: 5663 737 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3115 3287 3337 3846 3115 Size: 172 50 509 6047 Fragments arranged by size: 6047 509 172 50 FspI TGC'GCA Cuts at: 5284 5284 Size: 6778 HaeII r_GCGC'y Cuts at: 1678 5925 1678 Size: 4247 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2849 2881 3719 3751 5419 Size: 32 135 32 1277 32 838 32 1668 Cuts at: 5419 5451 5493 5525 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1668 1277 838 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2604 3664 4122 4368 669 Size: 988 281 666 1060 458 246 3079 Fragments arranged by size: 3079 1060 988 666 458 281 246 HindIII A'AGCT_T Cuts at: 748 3029 748 Size: 2281 4497 HpaI GTT'AAC Cuts at: 3664 4368 3664 Size: 704 6074 KpnI G_GTAC'C Cuts at: 4103 4103 Size: 6778 MfeI C'AATT_G Cuts at: 3195 4377 3195 Size: 1182 5596 MluI A'CGCG_T Cuts at: 6654 6654 Size: 6778 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5892 6076 1305 Size: 4587 184 2007 Fragments arranged by size: 4587 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3026 10 Size: 54 1425 343 1194 3762 Fragments arranged by size: 3762 1425 1194 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2170 3964 4736 5095 5254 518 Size: 648 1004 1794 772 359 159 2042 Fragments arranged by size: 2042 1794 1004 772 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2943 4194 4871 6019 6264 1606 Size: 1337 1251 677 1148 245 2120 Fragments arranged by size: 2120 1337 1251 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6226 NdeI CA'TA_TG Cuts at: 387 2083 387 Size: 1696 5082 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6778 NotI GC'GGCC_GC Cuts at: 4225 4225 Size: 6778 NsiI A_TGCA'T Cuts at: 2488 3601 2488 Size: 1113 5665 NspI r_CATG'y Cuts at: 1132 1229 2490 2540 4136 5681 1132 Size: 97 1261 50 1596 1545 2229 Fragments arranged by size: 2229 1596 1545 1261 97 50 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6778 PciI A'CATG_T Cuts at: 5677 5677 Size: 6778 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6778 PmeI GTTT'AAAC Cuts at: 4082 4082 Size: 6778 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4847 4879 6360 6392 4847 Size: 32 1481 32 5233 Fragments arranged by size: 5233 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6778 PsiI TTA'TAA Cuts at: 4348 4348 Size: 6778 PstI C_TGCA'G Cuts at: 830 4130 830 Size: 3300 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6778 PvuII CAG'CTG Cuts at: 2943 2943 Size: 6778 SacI G_AGCT'C Cuts at: 3137 3137 Size: 6778 SalI G'TCGA_C Cuts at: 1936 4120 1936 Size: 2184 4594 SbfI CC_TGCA'GG Cuts at: 4130 4130 Size: 6778 ScaI AGT'ACT Cuts at: 5026 6738 5026 Size: 1712 5066 SfcI C'TryA_G Cuts at: 826 1045 4126 5261 5942 6133 826 Size: 219 3081 1135 681 191 1471 Fragments arranged by size: 3081 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6778 SmaI CCC'GGG Cuts at: 1872 4105 1872 Size: 2233 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3032 3100 4882 5783 6045 820 Size: 197 926 1089 68 1782 901 262 1553 Fragments arranged by size: 1782 1553 1089 926 901 262 197 68 SnaBI TAC'GTA Cuts at: 493 3410 4569 493 Size: 2917 1159 2702 Fragments arranged by size: 2917 2702 1159 SpeI A'CTAG_T Cuts at: 152 2203 152 Size: 2051 4727 SphI G_CATG'C Cuts at: 1132 4136 1132 Size: 3004 3774 SspI AAT'ATT Cuts at: 5 52 2095 2479 3523 4702 5 Size: 47 2043 384 1044 1179 2081 Fragments arranged by size: 2081 2043 1179 1044 384 47 StuI AGG'CCT Cuts at: 1517 2563 1517 Size: 1046 5732 StyI C'CwwG_G Cuts at: 513 1065 3395 4217 513 Size: 552 2330 822 3074 Fragments arranged by size: 3074 2330 822 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4805 4822 4975 5160 5605 325 Size: 1255 3225 17 153 185 445 1498 Fragments arranged by size: 3225 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2489 2539 2582 Size: 274 80 33 51 1443 512 50 43 Cuts at: 2582 3802 5024 6736 96 Size: 1220 1222 1712 138 Fragments arranged by size: 1712 1443 1222 1220 512 274 138 80 51 50 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2876 6738 6770 2844 Size: 32 3862 32 2852 Fragments arranged by size: 3862 2852 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6778 XbaI T'CTAG_A Cuts at: 4114 4114 Size: 6778 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3617 3617 Size: 6778 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6778 XmaI C'CCGG_G Cuts at: 1870 4103 1870 Size: 2233 4545 XmnI GAAnn'nnTTC Cuts at: 4907 4907 Size: 6778 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4586 276 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI