Restriction map for
pFN21AB9606
(Linear) (Six-base) MAPSORT of: KIBB9606.seq Check: 8879 from: 1 to: 1077
KIBB9606.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
Acc65I G'GTAC_C
Cuts at: 0 818 1077
Size: 818 259
AhdI GACnn_n'nnGTC
Cuts at: 0 288 1077
Size: 288 789
AlwNI CAG_nnn'CTG
Cuts at: 0 61 1017 1077
Size: 61 956 60
Fragments arranged by size:
956 61 60
AvaI C'yCGr_G
Cuts at: 0 121 337 1077
Size: 121 216 740
Fragments arranged by size:
740 216 121
BamHI G'GATC_C
Cuts at: 0 563 1077
Size: 563 514
BanI G'GyrC_C
Cuts at: 0 117 818 1077
Size: 117 701 259
Fragments arranged by size:
701 259 117
BanII G_rGCy'C
Cuts at: 0 396 860 1077
Size: 396 464 217
Fragments arranged by size:
464 396 217
BclI T'GATC_A
Cuts at: 0 633 1077
Size: 633 444
BglI GCCn_nnn'nGGC
Cuts at: 0 101 1077
Size: 101 976
Bme1580I G_kGCm'C
Cuts at: 0 122 1077
Size: 122 955
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 1077
Size: 153 924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 949 994 1000 1077
Size: 901 48 45 6 77
Fragments arranged by size:
901 77 48 45 6
Bpu10I CC'TnA_GC
Cuts at: 0 89 1077
Size: 89 988
BsaHI Gr'CG_yC
Cuts at: 0 334 465 1077
Size: 334 131 612
Fragments arranged by size:
612 334 131
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 139 430 661 754 1077
Size: 124 15 291 231 93 323
Fragments arranged by size:
323 291 231 124 93 15
BseYI C'CCAG_C
Cuts at: 0 356 441 942 1077
Size: 356 85 501 135
Fragments arranged by size:
501 356 135 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1077
Size: 1018 59
BsiEI CG_ry'CG
Cuts at: 0 599 1077
Size: 599 478
BsiHKAI G_wGCw'C
Cuts at: 0 860 1077
Size: 860 217
BsiWI C'GTAC_G
Cuts at: 0 809 1077
Size: 809 268
BsmBI CGTCTCn'nnnn_
Cuts at: 0 456 799 1077
Size: 456 343 278
Fragments arranged by size:
456 343 278
Bsp1286I G_dGCh'C
Cuts at: 0 122 396 860 1077
Size: 122 274 464 217
Fragments arranged by size:
464 274 217 122
BspHI T'CATG_A
Cuts at: 0 366 1077
Size: 366 711
BsrBI CCG'CTC
Cuts at: 0 114 816 1077
Size: 114 702 261
Fragments arranged by size:
702 261 114
BsrFI r'CCGG_y
Cuts at: 0 315 594 664 917 989 1037 1077
Size: 315 279 70 253 72 48 40
Fragments arranged by size:
315 279 253 72 70 48 40
BsrGI T'GTAC_A
Cuts at: 0 52 1077
Size: 52 1025
BssHII G'CGCG_C
Cuts at: 0 996 1077
Size: 996 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 166 387 1077
Size: 166 221 690
Fragments arranged by size:
690 221 166
BstYI r'GATC_y
Cuts at: 0 563 654 1077
Size: 563 91 423
Fragments arranged by size:
563 423 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 725 770 1077
Size: 725 45 307
Fragments arranged by size:
725 307 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 200 235 1077
Size: 200 35 842
Fragments arranged by size:
842 200 35
EaeI y'GGCC_r
Cuts at: 0 2 168 1077
Size: 2 166 909
Fragments arranged by size:
909 166 2
EarI CTCTTCn'nnn_
Cuts at: 0 71 407 512 1077
Size: 71 336 105 565
Fragments arranged by size:
565 336 105 71
EcoICRI GAG'CTC
Cuts at: 0 858 1077
Size: 858 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 949 994 1000 1077
Size: 901 48 45 6 77
Fragments arranged by size:
901 77 48 45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 401 1077
Size: 256 145 676
Fragments arranged by size:
676 256 145
HaeII r_GCGC'y
Cuts at: 0 96 1057 1077
Size: 96 961 20
Fragments arranged by size:
961 96 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 1077
Size: 274 32 771
Fragments arranged by size:
771 274 32
KpnI G_GTAC'C
Cuts at: 0 822 1077
Size: 822 255
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1077
Size: 722 355
MspA1I CmG'CkG
Cuts at: 0 295 441 792 1014 1077
Size: 295 146 351 222 63
Fragments arranged by size:
351 295 222 146 63
NaeI GCC'GGC
Cuts at: 0 317 1077
Size: 317 760
NgoMIV G'CCGG_C
Cuts at: 0 315 1077
Size: 315 762
PfoI T'CCnGG_A
Cuts at: 0 71 1077
Size: 71 1006
PshAI GACnn'nnGTC
Cuts at: 0 468 1077
Size: 468 609
PstI C_TGCA'G
Cuts at: 0 791 839 1077
Size: 791 48 238
Fragments arranged by size:
791 238 48
PvuII CAG'CTG
Cuts at: 0 441 1014 1077
Size: 441 573 63
Fragments arranged by size:
573 441 63
SacI G_AGCT'C
Cuts at: 0 860 1077
Size: 860 217
SapI GCTCTTCn'nnn_
Cuts at: 0 71 1077
Size: 71 1006
SfcI C'TryA_G
Cuts at: 0 787 835 1077
Size: 787 48 242
Fragments arranged by size:
787 242 48
StuI AGG'CCT
Cuts at: 0 232 1077
Size: 232 845
StyI C'CwwG_G
Cuts at: 0 584 1077
Size: 584 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 934 1036 1077
Size: 154 780 102 41
Fragments arranged by size:
780 154 102 41
TatI w'GTAC_w
Cuts at: 0 52 671 1077
Size: 52 619 406
Fragments arranged by size:
619 406 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 1077
Size: 121 32 924
Fragments arranged by size:
924 121 32
XmnI GAAnn'nnTTC
Cuts at: 0 529 1077
Size: 529 548
Enzymes that do cut:
Acc65I AhdI AlwNI AvaI BamHI BanI BanII BclI
BglI Bme1580I BmrI BpmI Bpu10I BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BsrBI
BsrFI BsrGI BssHII BstXI BstYI BtgZI CspCI EaeI
EarI EcoICRI Eco57MI EcoNI HaeII Hin4I KpnI MmeI
MspA1I NaeI NgoMIV PfoI PshAI PstI PvuII SacI
SapI SfcI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsmI BspEI BspMI
BsrDI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtsI ClaI DraI DraIII DrdI EagI EciI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI MscI MslI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI