(Circular) (Six-base) MAPSORT of: pFN21AB9603.seq Check: 8239 from: 1 to: 5740 pFN21AB9603 5740 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2770 2817 3803 5187 5455 1632 Size: 1138 47 986 1384 268 1917 Fragments arranged by size: 1917 1384 1138 986 268 47 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 2397 4639 5616 1203 Size: 1194 2242 977 1327 Fragments arranged by size: 2242 1327 1194 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 2370 3483 5055 2370 Size: 1113 1572 3055 Fragments arranged by size: 3055 1572 1113 ApaI G_GGCC'C Cuts at: 2450 2458 2546 2582 2871 2450 Size: 8 88 36 289 5319 Fragments arranged by size: 5319 289 88 36 8 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 3049 3275 3427 5536 1493 Size: 1556 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 226 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2125 2201 2450 2539 2722 2978 Size: 73 129 53 76 249 89 183 256 Cuts at: 2978 3065 1870 Size: 87 4545 Fragments arranged by size: 4545 256 249 183 129 89 87 76 73 53 AvrII C'CTAG_G Cuts at: 2848 2848 Size: 5740 BamHI G'GATC_C Cuts at: 2982 3070 3437 2982 Size: 88 367 5285 Fragments arranged by size: 5285 367 88 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2332 2421 2614 2855 2885 Size: 325 217 627 545 89 193 241 30 Cuts at: 2885 3061 4516 618 Size: 176 1455 1842 Fragments arranged by size: 1842 1455 627 545 325 241 217 193 176 89 30 BanII G_rGCy'C Cuts at: 1391 2450 2458 2540 2546 2582 2768 2871 3059 Size: 1059 8 82 6 36 186 103 188 Cuts at: 3059 1391 Size: 4072 Fragments arranged by size: 4072 1059 188 186 103 82 36 8 6 BbeI G_GCGC'C Cuts at: 2425 2618 2425 Size: 193 5547 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4769 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2520 2554 3931 3965 1903 Size: 34 583 34 1377 34 3678 Fragments arranged by size: 3678 1377 583 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2108 2462 4351 Size: 107 122 71 631 605 436 354 1889 Cuts at: 4351 136 Size: 1525 Fragments arranged by size: 1889 1525 631 605 436 354 122 107 71 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 2337 2450 2458 2546 2582 2871 3801 4957 Size: 547 113 8 88 36 289 930 1156 Cuts at: 4957 1790 Size: 2573 Fragments arranged by size: 2573 1156 930 547 289 113 88 36 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3471 4429 442 Size: 416 53 83 399 24 2054 958 1753 Fragments arranged by size: 2054 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2893 4400 1239 Size: 1654 1507 2579 Fragments arranged by size: 2579 1654 1507 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2071 2132 4403 882 Size: 617 572 61 2271 2219 Fragments arranged by size: 2271 2219 617 572 61 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2422 2615 3547 3929 Size: 53 83 186 936 889 193 932 382 Cuts at: 3929 4564 275 Size: 635 1451 Fragments arranged by size: 1451 936 932 889 635 382 193 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2070 2100 1178 Size: 30 165 30 667 30 4818 Fragments arranged by size: 4818 667 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2896 1186 Size: 1710 4030 BseYI C'CCAG_C Cuts at: 4943 5439 4943 Size: 496 5244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2354 2685 2694 2878 3190 3951 Size: 275 186 353 331 9 184 312 761 Cuts at: 3951 4979 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 353 331 312 275 186 184 9 BsiHKAI G_wGCw'C Cuts at: 2768 3801 3886 4957 2768 Size: 1033 85 1071 3551 Fragments arranged by size: 3551 1071 1033 85 BsmI GAATG_Cn' Cuts at: 3251 3344 3251 Size: 93 5647 BsmBI CGTCTCn'nnnn_ Cuts at: 2477 2477 Size: 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2450 2458 2540 2546 2582 2768 Size: 399 547 113 8 82 6 36 186 Cuts at: 2768 2871 3059 3801 3886 4957 1391 Size: 103 188 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 547 399 188 186 113 103 85 82 36 8 6 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3001 3095 844 Size: 2157 94 3489 Fragments arranged by size: 3489 2157 94 BsrBI CCG'CTC Cuts at: 1394 1823 2526 3627 1394 Size: 429 703 1101 3507 Fragments arranged by size: 3507 1101 703 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4235 4409 65 Size: 1183 2987 174 1396 Fragments arranged by size: 2987 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2271 2418 2611 4384 4571 1700 Size: 110 461 147 193 1773 187 2869 Fragments arranged by size: 2869 1773 461 193 187 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5740 BssHII G'CGCG_C Cuts at: 1925 2705 1925 Size: 780 4960 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2982 3070 3437 3822 3839 5291 5377 5389 Size: 1010 88 367 385 17 1452 86 12 Cuts at: 5389 5735 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1010 385 367 346 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 2181 2574 2685 513 Size: 552 1041 75 393 111 3568 Fragments arranged by size: 3568 1041 552 393 111 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2152 2161 487 Size: 683 56 926 9 4066 Fragments arranged by size: 4066 926 683 56 9 BtsI GCAGTG_nn' Cuts at: 3256 4050 4070 3256 Size: 794 20 4926 Fragments arranged by size: 4926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2324 2954 4747 809 Size: 507 1008 630 1793 1802 Fragments arranged by size: 1802 1793 1008 630 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2351 2627 Size: 54 1425 50 275 18 210 311 276 Cuts at: 2627 2682 2695 2875 2898 3187 4076 5705 8 Size: 55 13 180 23 289 889 1629 43 Fragments arranged by size: 1629 1425 889 311 289 276 275 210 180 55 54 50 43 23 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2351 2682 2875 3187 1537 Size: 275 539 331 193 312 4090 Fragments arranged by size: 4090 539 331 312 275 193 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2423 2756 3670 1353 Size: 668 402 333 914 3423 Fragments arranged by size: 3423 914 668 402 333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2285 2598 2910 4327 4701 4847 Size: 1064 138 696 313 312 1417 374 146 Cuts at: 4847 387 Size: 1280 Fragments arranged by size: 1417 1280 1064 696 374 313 312 146 138 EcoICRI GAG'CTC Cuts at: 2766 2766 Size: 5740 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2770 2817 2893 3803 4400 5187 5455 Size: 393 1138 47 76 910 597 787 268 Cuts at: 5455 1239 Size: 1524 Fragments arranged by size: 1524 1138 910 787 597 393 268 76 47 EcoO109I rG'GnC_Cy Cuts at: 2447 2454 2455 2472 2543 2579 2727 2447 Size: 7 1 17 71 36 148 5460 Fragments arranged by size: 5460 148 71 36 17 7 1 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 2425 2573 2618 4887 1678 Size: 747 148 45 2269 2531 Fragments arranged by size: 2531 2269 747 148 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 2588 2620 4381 Size: 32 135 32 498 32 486 32 1761 Cuts at: 4381 4413 4455 4487 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1761 498 486 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KasI G'GCGC_C Cuts at: 2421 2614 2421 Size: 193 5547 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2793 4854 5038 1305 Size: 1488 2061 184 2007 Fragments arranged by size: 2061 2007 1488 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3918 Fragments arranged by size: 3918 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 3698 4057 4216 518 Size: 648 1014 1518 359 159 2042 Fragments arranged by size: 2042 1518 1014 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2108 2388 2576 2681 3156 3833 4981 5226 Size: 502 280 188 105 475 677 1148 245 Cuts at: 5226 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 502 475 280 245 188 105 NaeI GCC'GGC Cuts at: 2613 2613 Size: 5740 NarI GG'CG_CC Cuts at: 2422 2615 2422 Size: 193 5547 NcoI C'CATG_G Cuts at: 513 1065 2181 513 Size: 552 1116 4072 Fragments arranged by size: 4072 1116 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NgoMIV G'CCGG_C Cuts at: 2611 2611 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 2401 2957 3098 4643 1132 Size: 97 1172 556 141 1545 2229 Fragments arranged by size: 2229 1545 1172 556 141 97 PasI CC'CwG_GG Cuts at: 1348 2671 1348 Size: 1323 4417 PciI A'CATG_T Cuts at: 2397 4639 2397 Size: 2242 3498 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5740 PfoI T'CCnGG_A Cuts at: 2190 2592 2190 Size: 402 5338 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2727 2727 Size: 5740 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PspOMI G'GGCC_C Cuts at: 2446 2454 2542 2578 2867 2446 Size: 8 88 36 289 5319 Fragments arranged by size: 5319 289 88 36 8 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2399 2431 2399 Size: 32 5708 PstI C_TGCA'G Cuts at: 830 2565 3012 3092 830 Size: 1735 447 80 3478 Fragments arranged by size: 3478 1735 447 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 SacI G_AGCT'C Cuts at: 2768 2768 Size: 5740 SacII CC_GC'GG Cuts at: 2109 2577 2109 Size: 468 5272 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SanDI GG'GwC_CC Cuts at: 2727 2727 Size: 5740 SapI GCTCTTCn'nnn_ Cuts at: 2756 2756 Size: 5740 SbfI CC_TGCA'GG Cuts at: 2565 3012 3092 2565 Size: 447 80 5213 Fragments arranged by size: 5213 447 80 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 2158 2561 3008 3088 4223 4904 5095 Size: 219 1113 403 447 80 1135 681 191 Cuts at: 5095 826 Size: 1471 Fragments arranged by size: 1471 1135 1113 681 447 403 219 191 80 SfiI GGCCn_nnn'nGGCC Cuts at: 2462 2462 Size: 5740 SfoI GGC'GCC Cuts at: 2423 2616 2423 Size: 193 5547 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 2611 1700 Size: 911 4829 SmaI CCC'GGG Cuts at: 1872 2452 2724 3067 1872 Size: 580 272 343 4545 Fragments arranged by size: 4545 580 343 272 SmlI C'TyrA_G Cuts at: 820 1017 1943 3844 4745 5007 820 Size: 197 926 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2181 2848 3179 513 Size: 552 1116 667 331 3074 Fragments arranged by size: 3074 1116 667 552 331 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2873 3767 3784 3937 4122 4567 325 Size: 1255 1293 894 17 153 185 445 1498 Fragments arranged by size: 1498 1293 1255 894 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3986 5698 96 Size: 274 80 33 51 1443 2009 1712 138 Fragments arranged by size: 2009 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 2638 1316 Size: 1322 4418 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 2450 2722 3065 1870 Size: 580 272 343 4545 Fragments arranged by size: 4545 580 343 272 XmnI GAAnn'nnTTC Cuts at: 3869 3869 Size: 5740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PvuII RsrII SexAI SrfI SwaI XcmI