(Circular) (Six-base) MAPSORT of: pFN21AB9600.seq Check: 8552 from: 1 to: 5824 pFN21AB9600 5824 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2637 3167 1937 Size: 700 530 4594 Fragments arranged by size: 4594 700 530 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3887 5271 5539 1632 Size: 2255 1384 268 1917 Fragments arranged by size: 2255 1917 1384 268 AfeI AGC'GCT Cuts at: 2261 2447 2456 3009 2261 Size: 186 9 553 5076 Fragments arranged by size: 5076 553 186 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 2409 4723 5700 1203 Size: 1206 2314 977 1327 Fragments arranged by size: 2314 1327 1206 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2447 1184 Size: 1263 4561 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2396 2428 2396 Size: 32 5792 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaI G_GGCC'C Cuts at: 2718 2733 2812 2855 2718 Size: 15 79 43 5687 Fragments arranged by size: 5687 79 43 15 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2365 2581 3149 1870 Size: 73 270 152 216 568 4545 Fragments arranged by size: 4545 568 270 216 152 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2133 2557 2585 2762 2876 Size: 325 217 627 346 424 28 177 114 Cuts at: 2876 3059 3145 4600 618 Size: 183 86 1455 1842 Fragments arranged by size: 1842 1455 627 424 346 325 217 183 177 114 86 28 BanII G_rGCy'C Cuts at: 1391 2718 2733 2812 2855 3143 1391 Size: 1327 15 79 43 288 4072 Fragments arranged by size: 4072 1327 288 79 43 15 BbeI G_GCGC'C Cuts at: 2137 2589 2766 2880 3063 2137 Size: 452 177 114 183 4898 Fragments arranged by size: 4898 452 183 177 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2665 928 Size: 971 766 4087 Fragments arranged by size: 4087 971 766 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2816 2837 2859 Size: 107 122 71 631 605 1144 21 22 Cuts at: 2859 4435 136 Size: 1576 1525 Fragments arranged by size: 1576 1525 1144 631 605 122 107 71 22 21 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 2699 3241 2699 Size: 542 5282 Bme1580I G_kGCm'C Cuts at: 1790 2562 2718 2733 2812 2855 3885 5041 1790 Size: 772 156 15 79 43 1030 1156 2573 Fragments arranged by size: 2573 1156 1030 772 156 79 43 15 BmgBI CAC'GTC Cuts at: 1318 2988 1318 Size: 1670 4154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 3555 4513 Size: 416 53 83 399 24 975 1163 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1163 975 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2616 4484 1239 Size: 1377 1868 2579 Fragments arranged by size: 2579 1868 1377 Bpu10I CC'TnA_GC Cuts at: 2688 2688 Size: 5824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2324 3949 4814 5112 2324 Size: 1625 865 298 3036 Fragments arranged by size: 3036 1625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2954 4487 882 Size: 617 1455 1533 2219 Fragments arranged by size: 2219 1533 1455 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2134 2586 2651 2763 Size: 53 83 186 936 601 452 65 112 Cuts at: 2763 2877 3060 3631 4013 4648 275 Size: 114 183 571 382 635 1451 Fragments arranged by size: 1451 936 635 601 571 452 382 186 183 114 112 83 65 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 1178 Size: 30 165 30 993 30 4576 Fragments arranged by size: 4576 993 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5824 BseYI C'CCAG_C Cuts at: 2230 2710 2932 3010 5027 5523 2230 Size: 480 222 78 2017 496 2531 Fragments arranged by size: 2531 2017 496 480 222 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2785 3075 1163 Size: 573 1049 290 3912 Fragments arranged by size: 3912 1049 573 290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2362 2696 2816 3274 4035 5063 Size: 275 186 361 334 120 458 761 1028 Cuts at: 5063 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 458 361 334 275 186 120 BsiHKAI G_wGCw'C Cuts at: 3885 3970 5041 3885 Size: 85 1071 4668 Fragments arranged by size: 4668 1071 85 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 BsmBI CGTCTCn'nnnn_ Cuts at: 2069 2069 Size: 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2562 2718 2733 2812 2855 3143 3885 Size: 399 772 156 15 79 43 288 742 Cuts at: 3885 3970 5041 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 772 742 399 288 156 85 79 43 15 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3179 844 Size: 2335 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2019 2421 2692 2698 3711 1394 Size: 429 196 402 271 6 1013 3507 Fragments arranged by size: 3507 1013 429 402 271 196 6 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2357 2759 2780 2815 2879 4468 4655 Size: 110 547 402 21 35 64 1589 187 Cuts at: 4655 1700 Size: 2869 Fragments arranged by size: 2869 1589 547 402 187 110 64 35 21 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 2567 1925 Size: 642 5182 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5824 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2343 3154 3521 3906 3923 5375 5461 5473 Size: 371 811 367 385 17 1452 86 12 Cuts at: 5473 5819 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 811 385 371 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2109 2116 2193 2646 2964 3120 513 Size: 552 1044 7 77 453 318 156 3217 Fragments arranged by size: 3217 1044 552 453 318 156 77 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2164 2173 2185 487 Size: 683 56 938 9 12 4126 Fragments arranged by size: 4126 938 683 56 12 9 BtsI GCAGTG_nn' Cuts at: 2890 2953 3340 4134 4154 2890 Size: 63 387 794 20 4560 Fragments arranged by size: 4560 794 387 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2359 2619 Size: 54 1425 50 275 18 366 163 260 Cuts at: 2619 2693 2813 2817 2905 2995 3271 4160 5789 Size: 74 120 4 88 90 276 889 1629 Cuts at: 5789 8 Size: 43 Fragments arranged by size: 1629 1425 889 366 276 275 260 163 120 90 88 74 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2359 2693 2813 3271 1537 Size: 275 547 334 120 458 4090 Fragments arranged by size: 4090 547 458 334 275 120 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 3754 1353 Size: 705 1696 3423 Fragments arranged by size: 3423 1696 705 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2297 2746 2818 3106 4411 4785 Size: 1064 138 708 449 72 288 1305 374 Cuts at: 4785 4931 387 Size: 146 1280 Fragments arranged by size: 1305 1280 1064 708 449 374 288 146 138 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2616 3887 4484 5271 5539 1239 Size: 393 984 1271 597 787 268 1524 Fragments arranged by size: 1524 1271 984 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2095 2714 2729 2808 2852 2918 2095 Size: 619 15 79 44 66 5001 Fragments arranged by size: 5001 619 79 66 44 15 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FseI GG_CCGG'CC Cuts at: 2361 2819 2361 Size: 458 5366 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2137 2146 2221 2263 2390 2449 2458 2589 Size: 459 9 75 42 127 59 9 131 Cuts at: 2589 2766 2880 3011 3063 4971 1678 Size: 177 114 131 52 1908 2531 Fragments arranged by size: 2531 1908 459 177 131 131 127 114 75 59 52 42 9 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373 Size: 32 135 32 2893 32 42 32 2626 Fragments arranged by size: 2893 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KasI G'GCGC_C Cuts at: 2133 2585 2762 2876 3059 2133 Size: 452 177 114 183 4898 Fragments arranged by size: 4898 452 183 177 114 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2198 10 Size: 54 1425 343 366 3636 Fragments arranged by size: 3636 1425 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 3782 4141 4300 518 Size: 648 1026 1590 359 159 2042 Fragments arranged by size: 2042 1590 1026 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2648 2900 2936 3240 3917 5065 5310 Size: 512 530 252 36 304 677 1148 245 Cuts at: 5310 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 530 512 304 252 245 36 NaeI GCC'GGC Cuts at: 2359 2761 2817 2881 2359 Size: 402 56 64 5302 Fragments arranged by size: 5302 402 64 56 NarI GG'CG_CC Cuts at: 2134 2586 2763 2877 3060 2134 Size: 452 177 114 183 4898 Fragments arranged by size: 4898 452 183 177 114 NcoI C'CATG_G Cuts at: 513 1065 2193 3120 513 Size: 552 1128 927 3217 Fragments arranged by size: 3217 1128 927 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2357 2759 2815 2879 2357 Size: 402 56 64 5302 Fragments arranged by size: 5302 402 64 56 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 2693 3271 2693 Size: 578 5246 NspI r_CATG'y Cuts at: 1132 1229 2413 2488 3182 4727 1132 Size: 97 1184 75 694 1545 2229 Fragments arranged by size: 2229 1545 1184 694 97 75 PasI CC'CwG_GG Cuts at: 1348 2099 1348 Size: 751 5073 PciI A'CATG_T Cuts at: 2409 4723 2409 Size: 2314 3510 PflMI CCAn_nnn'nTGG Cuts at: 1108 2292 1108 Size: 1184 4640 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2396 2428 3893 3925 5406 5438 2396 Size: 32 1465 32 1481 32 2782 Fragments arranged by size: 2782 1481 1465 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2095 2918 2095 Size: 823 5001 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2714 2729 2808 2851 2714 Size: 15 79 43 5687 Fragments arranged by size: 5687 79 43 15 PstI C_TGCA'G Cuts at: 830 2154 3176 830 Size: 1324 1022 3478 Fragments arranged by size: 3478 1324 1022 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 SacII CC_GC'GG Cuts at: 2119 2649 2119 Size: 530 5294 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SanDI GG'GwC_CC Cuts at: 2095 2918 2095 Size: 823 5001 SapI GCTCTTCn'nnn_ Cuts at: 2058 2058 Size: 5824 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2150 2170 3172 4307 4988 5179 826 Size: 219 1105 20 1002 1135 681 191 1471 Fragments arranged by size: 1471 1135 1105 1002 681 219 191 20 SfiI GGCCn_nnn'nGGCC Cuts at: 2816 2816 Size: 5824 SfoI GGC'GCC Cuts at: 2135 2587 2764 2878 3061 2135 Size: 452 177 114 183 4898 Fragments arranged by size: 4898 452 183 177 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 2759 1700 Size: 1059 4765 SmaI CCC'GGG Cuts at: 1872 2367 2583 3151 1872 Size: 495 216 568 4545 Fragments arranged by size: 4545 568 495 216 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 3928 4829 5091 820 Size: 197 926 396 1589 901 262 1553 Fragments arranged by size: 1589 1553 926 901 396 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2193 3120 3263 513 Size: 552 1128 927 143 3074 Fragments arranged by size: 3074 1128 927 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3851 3868 4021 4206 4651 325 Size: 1255 2271 17 153 185 445 1498 Fragments arranged by size: 2271 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4070 5782 96 Size: 274 80 33 51 1443 2093 1712 138 Fragments arranged by size: 2093 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 2365 2581 3149 1870 Size: 495 216 568 4545 Fragments arranged by size: 4545 568 495 216 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SexAI SrfI SwaI XcmI