(Circular) (Six-base) MAPSORT of: pFN21AB9585.seq Check: 8292 from: 1 to: 5740 pFN21AB9585 5740 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2212 3803 5187 5455 1632 Size: 580 1591 1384 268 1917 Fragments arranged by size: 1917 1591 1384 580 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 3483 5055 3483 Size: 1572 4168 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 3049 3275 3427 5536 1493 Size: 1556 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 226 152 AscI GG'CGCG_CC Cuts at: 2253 2253 Size: 5740 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2165 3008 3065 1870 Size: 73 222 843 57 4545 Fragments arranged by size: 4545 843 222 73 57 BamHI G'GATC_C Cuts at: 3070 3437 3070 Size: 367 5373 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2269 2433 2578 2650 Size: 325 217 627 359 123 164 145 72 Cuts at: 2650 2731 2767 3061 4516 618 Size: 81 36 294 1455 1842 Fragments arranged by size: 1842 1455 627 359 325 294 217 164 145 123 81 72 36 BanII G_rGCy'C Cuts at: 1391 2523 2547 2717 2922 2976 3015 3059 1391 Size: 1132 24 170 205 54 39 44 4072 Fragments arranged by size: 4072 1132 205 170 54 44 39 24 BbeI G_GCGC'C Cuts at: 2150 2273 2437 2582 2735 2771 2150 Size: 123 164 145 153 36 5119 Fragments arranged by size: 5119 164 153 145 123 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2804 928 Size: 971 905 3864 Fragments arranged by size: 3864 971 905 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2833 2867 3931 3965 1903 Size: 34 50 34 812 34 1064 34 3678 Fragments arranged by size: 3678 1064 812 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 2703 2765 Size: 107 122 71 631 605 472 559 62 Cuts at: 2765 4351 136 Size: 1586 1525 Fragments arranged by size: 1586 1525 631 605 559 472 122 107 71 62 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 2975 3157 2975 Size: 182 5558 Bme1580I G_kGCm'C Cuts at: 1790 3801 4957 1790 Size: 2011 1156 2573 Fragments arranged by size: 2573 2011 1156 BmgBI CAC'GTC Cuts at: 1318 2682 1318 Size: 1364 4376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3471 4429 442 Size: 416 53 83 399 24 2054 958 1753 Fragments arranged by size: 2054 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2915 2936 4400 1239 Size: 1022 654 21 1464 2579 Fragments arranged by size: 2579 1464 1022 654 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2507 3865 4730 5028 2507 Size: 1358 865 298 3219 Fragments arranged by size: 3219 1358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4403 882 Size: 617 2904 2219 Fragments arranged by size: 2904 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2422 3531 493 Size: 711 1218 1109 2702 Fragments arranged by size: 2702 1218 1109 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 2270 2434 2579 Size: 53 83 186 936 614 123 164 145 Cuts at: 2579 2724 2732 2768 3547 3929 4564 275 Size: 145 8 36 779 382 635 1451 Fragments arranged by size: 1451 936 779 635 614 382 186 164 145 145 123 83 53 36 8 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2342 2354 2372 2384 2948 Size: 30 165 30 939 12 18 12 564 Cuts at: 2948 2978 1178 Size: 30 3940 Fragments arranged by size: 3940 939 564 165 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2323 2582 2677 3016 1186 Size: 1137 259 95 339 3910 Fragments arranged by size: 3910 1137 339 259 95 BseYI C'CCAG_C Cuts at: 2029 2191 2248 4943 5439 2029 Size: 162 57 2695 496 2330 Fragments arranged by size: 2695 2330 496 162 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2444 2468 1163 Size: 573 708 24 4435 Fragments arranged by size: 4435 708 573 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2409 2742 2928 3190 3951 4979 Size: 275 186 408 333 186 262 761 1028 Cuts at: 4979 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 408 333 275 262 186 186 BsiHKAI G_wGCw'C Cuts at: 2523 2922 2976 3801 3886 4957 2523 Size: 399 54 825 85 1071 3306 Fragments arranged by size: 3306 1071 825 399 85 54 BsiWI C'GTAC_G Cuts at: 2121 2616 2121 Size: 495 5245 BsmI GAATG_Cn' Cuts at: 3251 3344 3251 Size: 93 5647 BsmBI CGTCTCn'nnnn_ Cuts at: 2891 2891 Size: 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2523 2547 2717 2922 2976 3015 3059 Size: 399 733 24 170 205 54 39 44 Cuts at: 3059 3801 3886 4957 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 733 399 205 170 85 54 44 39 24 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2767 3095 844 Size: 1923 328 3489 Fragments arranged by size: 3489 1923 328 BsrBI CCG'CTC Cuts at: 1394 1823 2658 2938 3627 1394 Size: 429 835 280 689 3507 Fragments arranged by size: 3507 835 689 429 280 BsrDI GCAATG_nn' Cuts at: 65 1248 4235 4409 65 Size: 1183 2987 174 1396 Fragments arranged by size: 2987 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2183 2404 2764 4384 4571 1700 Size: 110 373 221 360 1620 187 2869 Fragments arranged by size: 2869 1620 373 360 221 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5740 BssHII G'CGCG_C Cuts at: 1925 2215 2253 2454 2626 1925 Size: 290 38 201 172 5039 Fragments arranged by size: 5039 290 201 172 38 BssSI C'ACGA_G Cuts at: 2210 3800 4812 2210 Size: 1590 1012 3138 Fragments arranged by size: 3138 1590 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2394 794 Size: 1600 4140 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstXI CCAn_nnnn'nTGG Cuts at: 2080 2080 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2829 3070 3437 3822 3839 5291 5377 5389 Size: 857 241 367 385 17 1452 86 12 Cuts at: 5389 5735 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 857 385 367 346 241 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2073 2142 2154 2277 2283 2547 2802 Size: 552 1008 69 12 123 6 264 255 Cuts at: 2802 513 Size: 3451 Fragments arranged by size: 3451 1008 552 264 255 123 69 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2836 487 Size: 683 56 1610 3391 Fragments arranged by size: 3391 1610 683 56 BtsI GCAGTG_nn' Cuts at: 3034 3256 4050 4070 3034 Size: 222 794 20 4704 Fragments arranged by size: 4704 794 222 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 2068 3044 2068 Size: 976 4764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2157 2280 Size: 54 1425 50 275 18 252 75 123 Cuts at: 2280 2406 2925 3031 3187 4076 5705 8 Size: 126 519 106 156 889 1629 43 Fragments arranged by size: 1629 1425 889 519 275 252 156 126 123 106 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2406 2925 3187 1537 Size: 275 594 519 262 4090 Fragments arranged by size: 4090 594 519 275 262 EarI CTCTTCn'nnn_ Cuts at: 1353 2372 3670 1353 Size: 1019 1298 3423 Fragments arranged by size: 3423 1298 1019 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 2295 2543 4327 4701 4847 Size: 1064 138 528 178 248 1784 374 146 Cuts at: 4847 387 Size: 1280 Fragments arranged by size: 1784 1280 1064 528 374 248 178 146 138 EcoICRI GAG'CTC Cuts at: 2521 2920 2974 2521 Size: 399 54 5287 Fragments arranged by size: 5287 399 54 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2261 2915 2936 3803 4400 5187 Size: 393 580 49 654 21 867 597 787 Cuts at: 5187 5455 1239 Size: 268 1524 Fragments arranged by size: 1524 867 787 654 597 580 393 268 49 21 EcoO109I rG'GnC_Cy Cuts at: 2026 2263 2911 2026 Size: 237 648 4855 Fragments arranged by size: 4855 648 237 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FspI TGC'GCA Cuts at: 2861 2888 4246 2861 Size: 27 1358 4355 Fragments arranged by size: 4355 1358 27 HaeII r_GCGC'y Cuts at: 1678 2150 2273 2437 2582 2735 2771 4887 1678 Size: 472 123 164 145 153 36 2116 2531 Fragments arranged by size: 2531 2116 472 164 153 145 123 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2354 2374 2386 2948 Size: 32 135 32 770 12 20 12 562 Cuts at: 2948 2980 4381 4413 4455 4487 1373 Size: 32 1401 32 42 32 2626 Fragments arranged by size: 2626 1401 770 562 135 42 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KasI G'GCGC_C Cuts at: 2146 2269 2433 2578 2731 2767 2146 Size: 123 164 145 153 36 5119 Fragments arranged by size: 5119 164 153 145 123 36 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 2397 4854 5038 1305 Size: 1080 12 2457 184 2007 Fragments arranged by size: 2457 2007 1080 184 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 2084 10 Size: 54 1425 343 252 3666 Fragments arranged by size: 3666 1425 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2855 3698 4057 4216 518 Size: 648 912 777 843 359 159 2042 Fragments arranged by size: 2042 912 843 777 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2144 2156 2248 2549 2667 2720 2962 3004 Size: 538 12 92 301 118 53 242 42 Cuts at: 3004 3156 3833 4981 5226 1606 Size: 152 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 538 301 245 242 152 118 92 53 42 12 NaeI GCC'GGC Cuts at: 2185 2406 2766 2185 Size: 221 360 5159 Fragments arranged by size: 5159 360 221 NarI GG'CG_CC Cuts at: 2147 2270 2434 2579 2732 2768 2147 Size: 123 164 145 153 36 5119 Fragments arranged by size: 5119 164 153 145 123 36 NcoI C'CATG_G Cuts at: 513 1065 2073 513 Size: 552 1008 4180 Fragments arranged by size: 4180 1008 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NgoMIV G'CCGG_C Cuts at: 2183 2404 2764 2183 Size: 221 360 5159 Fragments arranged by size: 5159 360 221 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 2037 3098 4643 1132 Size: 97 808 1061 1545 2229 Fragments arranged by size: 2229 1545 1061 808 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5740 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5740 PfoI T'CCnGG_A Cuts at: 2913 2913 Size: 5740 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 2911 2026 Size: 885 4855 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PstI C_TGCA'G Cuts at: 830 2946 3092 830 Size: 2116 146 3478 Fragments arranged by size: 3478 2116 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2962 3004 2962 Size: 42 5698 SacI G_AGCT'C Cuts at: 2523 2922 2976 2523 Size: 399 54 5287 Fragments arranged by size: 5287 399 54 SacII CC_GC'GG Cuts at: 2145 2157 2550 2145 Size: 12 393 5335 Fragments arranged by size: 5335 393 12 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SanDI GG'GwC_CC Cuts at: 2026 2026 Size: 5740 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 2942 3088 4223 4904 5095 826 Size: 219 1897 146 1135 681 191 1471 Fragments arranged by size: 1897 1471 1135 681 219 191 146 SfoI GGC'GCC Cuts at: 2148 2271 2435 2580 2733 2769 2148 Size: 123 164 145 153 36 5119 Fragments arranged by size: 5119 164 153 145 123 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 2183 2764 1700 Size: 483 581 4676 Fragments arranged by size: 4676 581 483 SmaI CCC'GGG Cuts at: 1872 2167 3067 1872 Size: 295 900 4545 Fragments arranged by size: 4545 900 295 SmlI C'TyrA_G Cuts at: 820 1017 1943 2522 3844 4745 5007 820 Size: 197 926 579 1322 901 262 1553 Fragments arranged by size: 1553 1322 926 901 579 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2073 2448 2727 3179 513 Size: 552 1008 375 279 452 3074 Fragments arranged by size: 3074 1008 552 452 375 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2502 3767 3784 3937 4122 4567 325 Size: 1255 922 1265 17 153 185 445 1498 Fragments arranged by size: 1498 1265 1255 922 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 3986 5698 Size: 274 80 33 51 1443 846 1163 1712 Cuts at: 5698 96 Size: 138 Fragments arranged by size: 1712 1443 1163 846 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2800 2011 Size: 789 4951 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 2165 3065 1870 Size: 295 900 4545 Fragments arranged by size: 4545 900 295 XmnI GAAnn'nnTTC Cuts at: 2993 3869 2993 Size: 876 4864 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI