Restriction map for
pFN21AB9572
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstXI BstZ17I
CelII CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MroI NarI NdeI NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 565 GACGCC G[GA]^CG[CT]C
AfaI 64 GTAC GT^AC
AocI 711 CCTGAGG CC^T[ACGT]AGG
Asp700I 1941 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 51 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1536 TGGCCA TGG^CCA
BamHI 1278 GGATCC G^GATCC
BanI 1594 GGTGCC G^G[CT][GA]CC
BbsI 1642 GAAGAC GAAGAC(2/6)
BbvCI 1740 CCTCAGC CCTCAGC(-5/-2)
BpuAI 1642 GAAGAC GAAGAC(2/6)
BsaHI 565 GACGCC G[GA]^CG[CT]C
BspMI 1084 GCAGGT ACCTGC(4/8)
BsrFI 79 GCCGGC [GA]^CCGG[CT]
BssSI 1115 CACGAG CACGAG(-5/-1)
BstBI 2201 TTCGAA TT^CGAA
BstEII 606 GGTCACC G^GT[ACGT]ACC
BstPI 606 GGTCACC G^GT[ACGT]ACC
Bsu36I 711 CCTGAGG CC^T[ACGT]AGG
Cfr10I 79 GCCGGC [GA]^CCGG[CT]
DraI 1285 TTTAAA TTT^AAA
EaeI 1536 TGGCCA [CT]^GGCC[GA]
Eco81I 711 CCTGAGG CC^T[ACGT]AGG
EcoNI 829 CCTTTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 606 GGTCACC G^GT[ACGT]ACC
EcoRV 481 GATATC GAT^ATC
EcoT22I 1475 ATGCAT ATGCA^T
HgaI 565 GACGC GACGC(5/10)
Hin1I 565 GACGCC G[GA]^CG[CT]C
MfeI 725 CAATTG C^AATTG
MluNI 1536 TGGCCA TGG^CCA
MscI 1536 TGGCCA TGG^CCA
MunI 725 CAATTG C^AATTG
NaeI 79 GCCGGC GCC^GGC
NgoMIV 79 GCCGGC G^CCGGC
NsiI 1475 ATGCAT ATGCA^T
NspI 1477 GCATGT [GA]CATG^[CT]
NspV 2201 TTCGAA TT^CGAA
PaeR7I 613 CTCGAG C^TCGAG
PflFI 51 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1629 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 64 GTAC GT^AC
SacI 942 GAGCTC GAGCT^C
SapI 1941 GAAGAGC GCTCTTC(1/4)
SfuI 2201 TTCGAA TT^CGAA
SgrAI 78 CGCCGGCG C[GA]^CCGG[CT]G
StuI 617 AGGCCT AGG^CCT
Tth111I 51 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1629 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 749 CCAACGCTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 613 CTCGAG C^TCGAG
XmnI 1941 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 67 CGCG CG^CG
1379 CGCG
ApaI 100 GGGCCC GGGCC^C
2282 GGGCCC
BcnI 883 CCCGG CC^[GC]GG
1841 CCCGG
BfaI 835 CTAG C^TAG
1157 CTAG
BpmI 571 CTCCAG CTGGAG(16/14)
1669 CTGGAG
BsaI 139 GAGACC GGTCTC(1/5)
1837 GAGACC
BsgI 34 GTGCAG GTGCAG(16/14)
2225 GTGCAG
BsmBI 1118 GAGACG CGTCTC(1/5)
1621 GAGACG
BstUI 67 CGCG CG^CG
1379 CGCG
HindIII 955 AAGCTT A^AGCTT
1018 AAGCTT
MaeI 835 CTAG C^TAG
1157 CTAG
MslI 514 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1339 CACGTTAATG
MspA1I 1589 CAGCTG C[AC]G^C[GT]G
1760 CAGCTG
MvnI 67 CGCG CG^CG
1379 CGCG
NciI 883 CCCGG CC^[GC]GG
1841 CCCGG
NcoI 1274 CCATGG C^CATGG
1961 CCATGG
PpuMI 255 AGGACCT [GA]G^G[AT]CC[CT]
2005 AGGTCCC
PvuII 1589 CAGCTG CAG^CTG
1760 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BanII
BbvI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII