(Circular) (Six-base) MAPSORT of: pFN21AB9572.seq Check: 3791 from: 1 to: 7069 pFN21AB9572 7069 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4879 278 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4412 1937 Size: 2475 4594 Acc65I G'GTAC_C Cuts at: 4390 4390 Size: 7069 AclI AA'CG_TT Cuts at: 1563 5196 5569 1563 Size: 3633 373 3063 Fragments arranged by size: 3633 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 2217 2340 2785 2829 4012 5132 6516 Size: 564 21 123 445 44 1183 1120 1384 Cuts at: 6516 6784 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1183 1120 564 445 268 123 44 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6872 AflIII A'CryG_T Cuts at: 1203 5968 6945 1203 Size: 4765 977 1327 Fragments arranged by size: 4765 1327 977 AgeI A'CCGG_T Cuts at: 5900 5900 Size: 7069 AhdI GACnn_n'nnGTC Cuts at: 5798 5798 Size: 7069 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2567 1184 Size: 1383 5686 AlwNI CAG_nnn'CTG Cuts at: 2029 2264 2636 3961 3985 4150 4812 6384 2029 Size: 235 372 1325 24 165 662 1572 2714 Fragments arranged by size: 2714 1572 1325 662 372 235 165 24 ApaI G_GGCC'C Cuts at: 2109 4291 2109 Size: 2182 4887 ApaLI G'TGCA_C Cuts at: 5126 6282 5126 Size: 1156 5913 ApoI r'AATT_y Cuts at: 1493 2404 2439 3413 4378 4604 4756 6865 1493 Size: 911 35 974 965 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 965 911 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 5623 160 Size: 872 4591 1606 Fragments arranged by size: 4591 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2120 2618 3489 4394 1870 Size: 73 165 12 498 871 905 4545 Fragments arranged by size: 4545 905 871 498 165 73 12 BamHI G'GATC_C Cuts at: 3283 4399 4766 3283 Size: 1116 367 5586 Fragments arranged by size: 5586 1116 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3599 4390 5845 618 Size: 325 217 627 1812 791 1455 1842 Fragments arranged by size: 1842 1812 1455 791 627 325 217 BanII G_rGCy'C Cuts at: 1391 2109 2951 3245 3728 4291 4388 1391 Size: 718 842 294 483 563 97 4072 Fragments arranged by size: 4072 842 718 563 483 294 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3654 928 Size: 971 1755 4343 Fragments arranged by size: 4343 1755 971 BbvCI CC'TCA_GC Cuts at: 3746 3746 Size: 7069 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5260 5294 1903 Size: 34 3323 34 3678 Fragments arranged by size: 3678 3323 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4961 6171 4961 Size: 1210 5859 BclI T'GATC_A Cuts at: 1548 1548 Size: 7069 BfrBI ATG'CAT Cuts at: 3482 3482 Size: 7069 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5680 136 Size: 107 122 71 631 605 4008 1525 Fragments arranged by size: 4008 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7064 7064 Size: 7069 BlpI GC'TnA_GC Cuts at: 4486 4486 Size: 7069 Bme1580I G_kGCm'C Cuts at: 1790 2109 2676 2774 4291 5130 6286 1790 Size: 319 567 98 1517 839 1156 2573 Fragments arranged by size: 2573 1517 1156 839 567 319 98 BmgBI CAC'GTC Cuts at: 1318 2058 3576 3630 1318 Size: 740 1518 54 4757 Fragments arranged by size: 4757 1518 740 54 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3047 3107 4800 Size: 416 53 83 399 24 1630 60 1693 Cuts at: 4800 5758 442 Size: 958 1753 Fragments arranged by size: 1753 1693 1630 958 416 399 83 60 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7069 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2970 4251 2970 Size: 1281 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2561 3695 5729 1239 Size: 1322 1134 2034 2579 Fragments arranged by size: 2579 2034 1322 1134 Bpu10I CC'TnA_GC Cuts at: 3746 4101 4200 3746 Size: 355 99 6615 Fragments arranged by size: 6615 355 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2176 2942 2984 5194 6059 6357 2176 Size: 766 42 2210 865 298 2888 Fragments arranged by size: 2888 2210 865 766 298 42 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2138 3836 5732 882 Size: 617 639 1698 1896 2219 Fragments arranged by size: 2219 1896 1698 639 617 BsaAI yAC'GTr Cuts at: 493 1204 4860 493 Size: 711 3656 2702 Fragments arranged by size: 3656 2702 711 BsaBI GATnn'nnATC Cuts at: 7063 7063 Size: 7069 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2571 4876 5258 5893 Size: 53 83 186 936 1038 2305 382 635 Cuts at: 5893 275 Size: 1451 Fragments arranged by size: 2305 1451 1038 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5502 5900 6174 6321 6732 5502 Size: 398 274 147 411 5839 Fragments arranged by size: 5839 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3037 3067 1178 Size: 30 165 30 1634 30 5180 Fragments arranged by size: 5180 1634 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2141 2751 2951 2959 3572 4145 1186 Size: 952 3 610 200 8 613 573 4110 Fragments arranged by size: 4110 952 613 610 573 200 8 3 BseYI C'CCAG_C Cuts at: 2675 2929 3179 4354 6272 6768 2675 Size: 254 250 1175 1918 496 2976 Fragments arranged by size: 2976 1918 1175 496 254 250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 4251 1163 Size: 573 324 2191 3981 Fragments arranged by size: 3981 2191 573 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4519 5280 6308 1540 Size: 275 186 2518 761 1028 2301 Fragments arranged by size: 2518 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2951 3774 4102 5130 5215 6286 2951 Size: 823 328 1028 85 1071 3734 Fragments arranged by size: 3734 1071 1028 823 328 85 BsmI GAATG_Cn' Cuts at: 4580 4673 4580 Size: 93 6976 BsmBI CGTCTCn'nnnn_ Cuts at: 3117 3620 3117 Size: 503 6566 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2676 2774 2951 3245 3728 3774 Size: 399 319 567 98 177 294 483 46 Cuts at: 3774 4102 4291 4388 5130 5215 6286 1391 Size: 328 189 97 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 483 399 328 319 294 189 177 98 97 85 46 BspEI T'CCGG_A Cuts at: 6732 6732 Size: 7069 BspHI T'CATG_A Cuts at: 4958 6688 4958 Size: 1730 5339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3080 4424 844 Size: 2236 1344 3489 Fragments arranged by size: 3489 2236 1344 BsrBI CCG'CTC Cuts at: 1394 1823 4956 1394 Size: 429 3133 3507 Fragments arranged by size: 3507 3133 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5564 5738 65 Size: 1183 4316 174 1396 Fragments arranged by size: 4316 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 5713 5900 1700 Size: 110 274 3629 187 2869 Fragments arranged by size: 3629 2869 274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7069 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7069 BssSI C'ACGA_G Cuts at: 3120 5129 6141 3120 Size: 2009 1012 4048 Fragments arranged by size: 4048 2009 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7069 BstBI TT'CG_AA Cuts at: 4207 5873 4207 Size: 1666 5403 BstEII G'GTnAC_C Cuts at: 1184 2611 1184 Size: 1427 5642 BstYI r'GATC_y Cuts at: 1972 2025 3157 3283 3738 4399 4766 5151 5168 Size: 53 1132 126 455 661 367 385 17 Cuts at: 5168 6620 6706 6718 7064 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1132 661 455 385 367 346 126 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2717 2717 Size: 7069 BtgI C'CryG_G Cuts at: 513 1065 2004 2018 2481 3279 3966 513 Size: 552 939 14 463 798 687 3616 Fragments arranged by size: 3616 939 798 687 552 463 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2603 487 Size: 683 56 1377 4953 Fragments arranged by size: 4953 1377 683 56 BtsI GCAGTG_nn' Cuts at: 2048 3322 3510 4585 5379 5399 2048 Size: 1274 188 1075 794 20 3718 Fragments arranged by size: 3718 1274 1075 794 188 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7069 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3646 3681 529 Size: 35 3082 35 3917 Fragments arranged by size: 3917 3082 35 35 DraI TTT'AAA Cuts at: 3292 4373 3292 Size: 1081 5988 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6076 809 Size: 507 4760 1802 Fragments arranged by size: 4760 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3541 4516 5405 Size: 54 1425 50 275 18 1711 975 889 Cuts at: 5405 7034 8 Size: 1629 43 Fragments arranged by size: 1711 1629 1425 975 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4516 1537 Size: 275 2704 4090 Fragments arranged by size: 4090 2704 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2805 3238 3783 3834 3941 4031 4161 4185 Size: 1452 433 545 51 107 90 130 24 Cuts at: 4185 4999 1353 Size: 814 3423 Fragments arranged by size: 3423 1452 814 545 433 130 107 90 51 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5656 6030 6176 387 Size: 1064 138 4067 374 146 1280 Fragments arranged by size: 4067 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2949 2949 Size: 7069 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2217 2340 2561 2785 2829 3695 Size: 393 564 21 123 221 224 44 866 Cuts at: 3695 4012 5132 5729 6516 6784 1239 Size: 317 1120 597 787 268 1524 Fragments arranged by size: 1524 1120 866 787 597 564 393 317 268 224 221 123 44 21 EcoNI CCTnn'n_nnAGG Cuts at: 2838 2838 Size: 7069 EcoO109I rG'GnC_Cy Cuts at: 2261 3694 3982 4011 4147 4287 4288 2261 Size: 1433 288 29 136 140 1 5042 Fragments arranged by size: 5042 1433 288 140 136 29 1 EcoRI G'AATT_C Cuts at: 4378 4756 4378 Size: 378 6691 EcoRV GAT'ATC Cuts at: 2488 2488 Size: 7069 FspI TGC'GCA Cuts at: 5575 5575 Size: 7069 HaeII r_GCGC'y Cuts at: 1678 6216 1678 Size: 4538 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2378 2410 3886 Size: 32 135 32 563 32 211 32 1476 Cuts at: 3886 3918 3983 4015 5710 5742 5784 5816 1373 Size: 32 65 32 1695 32 42 32 2626 Fragments arranged by size: 2626 1695 1476 563 211 135 65 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4413 4659 669 Size: 988 281 2475 246 3079 Fragments arranged by size: 3079 2475 988 281 246 HindIII A'AGCT_T Cuts at: 748 2960 3023 748 Size: 2212 63 4794 Fragments arranged by size: 4794 2212 63 HpaI GTT'AAC Cuts at: 4659 4659 Size: 7069 KpnI G_GTAC'C Cuts at: 4394 4394 Size: 7069 MfeI C'AATT_G Cuts at: 2730 4668 2730 Size: 1938 5131 MluI A'CGCG_T Cuts at: 6945 6945 Size: 7069 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2796 6183 6367 1305 Size: 1491 3387 184 2007 Fragments arranged by size: 3387 2007 1491 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3543 10 Size: 54 1425 343 1711 3536 Fragments arranged by size: 3536 1711 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2523 3348 5027 5386 5545 518 Size: 648 1357 825 1679 359 159 2042 Fragments arranged by size: 2042 1679 1357 825 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3596 3767 4485 5162 6310 6555 1606 Size: 1990 171 718 677 1148 245 2120 Fragments arranged by size: 2120 1990 1148 718 677 245 171 NaeI GCC'GGC Cuts at: 2086 2086 Size: 7069 NcoI C'CATG_G Cuts at: 513 1065 2004 3279 3966 513 Size: 552 939 1275 687 3616 Fragments arranged by size: 3616 1275 939 687 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7069 NgoMIV G'CCGG_C Cuts at: 2084 2084 Size: 7069 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7069 NotI GC'GGCC_GC Cuts at: 4516 4516 Size: 7069 NsiI A_TGCA'T Cuts at: 3484 3484 Size: 7069 NspI r_CATG'y Cuts at: 1132 1229 3486 4427 5972 1132 Size: 97 2257 941 1545 2229 Fragments arranged by size: 2257 2229 1545 941 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7069 PciI A'CATG_T Cuts at: 5968 5968 Size: 7069 PflMI CCAn_nnn'nTGG Cuts at: 1108 3640 1108 Size: 2532 4537 PfoI T'CCnGG_A Cuts at: 2577 2577 Size: 7069 PmeI GTTT'AAAC Cuts at: 4373 4373 Size: 7069 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5138 5170 6651 6683 5138 Size: 32 1481 32 5524 Fragments arranged by size: 5524 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2261 4011 2261 Size: 1750 5319 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7069 PsiI TTA'TAA Cuts at: 4639 4639 Size: 7069 PspOMI G'GGCC_C Cuts at: 2105 4287 2105 Size: 2182 4887 PspXI vC'TCGA_Gb Cuts at: 2618 2618 Size: 7069 PstI C_TGCA'G Cuts at: 830 2536 3216 3688 4296 4421 830 Size: 1706 680 472 608 125 3478 Fragments arranged by size: 3478 1706 680 608 472 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7069 PvuII CAG'CTG Cuts at: 3596 3767 3596 Size: 171 6898 SacI G_AGCT'C Cuts at: 2951 2951 Size: 7069 SalI G'TCGA_C Cuts at: 1936 4411 1936 Size: 2475 4594 SapI GCTCTTCn'nnn_ Cuts at: 3941 3941 Size: 7069 SbfI CC_TGCA'GG Cuts at: 4421 4421 Size: 7069 ScaI AGT'ACT Cuts at: 5317 7029 5317 Size: 1712 5357 SfcI C'TryA_G Cuts at: 826 1045 2532 3105 3212 3684 4292 4417 5552 Size: 219 1487 573 107 472 608 125 1135 Cuts at: 5552 6233 6424 826 Size: 681 191 1471 Fragments arranged by size: 1487 1471 1135 681 608 573 472 219 191 125 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 SgrAI Cr'CCGG_yG Cuts at: 1700 2084 1700 Size: 384 6685 SmaI CCC'GGG Cuts at: 1872 4396 1872 Size: 2524 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2155 2618 2957 2963 5173 6074 Size: 197 926 212 463 339 6 2210 901 Cuts at: 6074 6336 820 Size: 262 1553 Fragments arranged by size: 2210 1553 926 901 463 339 262 212 197 6 SnaBI TAC'GTA Cuts at: 493 4860 493 Size: 4367 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7069 SphI G_CATG'C Cuts at: 1132 4427 1132 Size: 3295 3774 SspI AAT'ATT Cuts at: 5 52 4993 5 Size: 47 4941 2081 Fragments arranged by size: 4941 2081 47 StuI AGG'CCT Cuts at: 1517 2624 1517 Size: 1107 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2784 3017 3279 3966 4508 513 Size: 552 939 780 233 262 687 542 3074 Fragments arranged by size: 3074 939 780 687 552 542 262 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3382 5096 5113 5266 5451 5896 325 Size: 1255 1802 1714 17 153 185 445 1498 Fragments arranged by size: 1802 1714 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5315 7027 96 Size: 274 80 33 51 1443 3338 1712 138 Fragments arranged by size: 3338 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3037 3069 7029 7061 3037 Size: 32 3960 32 3045 Fragments arranged by size: 3960 3045 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2059 1316 Size: 743 6326 XbaI T'CTAG_A Cuts at: 4405 4405 Size: 7069 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2761 2761 Size: 7069 XhoI C'TCGA_G Cuts at: 1943 2618 1943 Size: 675 6394 XmaI C'CCGG_G Cuts at: 1870 4394 1870 Size: 2524 4545 XmnI GAAnn'nnTTC Cuts at: 3950 5198 3950 Size: 1248 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4877 276 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I DraIII FalI FseI FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI