Restriction map for
pFN21AB9572
(Linear) (Six-base) MAPSORT of: KIBB9572.seq Check: 2788 from: 1 to: 2364
KIBB9572.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 212 335 780 824 2007 2364
Size: 191 21 123 445 44 1183 357
Fragments arranged by size:
1183 445 357 191 123 44 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 2364
Size: 562 1802
AlwNI CAG_nnn'CTG
Cuts at: 0 24 259 631 1956 1980 2145 2364
Size: 24 235 372 1325 24 165 219
Fragments arranged by size:
1325 372 235 219 165 24 24
ApaI G_GGCC'C
Cuts at: 0 104 2286 2364
Size: 104 2182 78
Fragments arranged by size:
2182 104 78
ApoI r'AATT_y
Cuts at: 0 399 434 1408 2364
Size: 399 35 974 956
Fragments arranged by size:
974 956 399 35
AvaI C'yCGr_G
Cuts at: 0 103 115 613 1484 2364
Size: 103 12 498 871 880
Fragments arranged by size:
880 871 498 103 12
BamHI G'GATC_C
Cuts at: 0 1278 2364
Size: 1278 1086
BanI G'GyrC_C
Cuts at: 0 1594 2364
Size: 1594 770
BanII G_rGCy'C
Cuts at: 0 104 946 1240 1723 2286 2364
Size: 104 842 294 483 563 78
Fragments arranged by size:
842 563 483 294 104 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 1649 2364
Size: 1649 715
BbvCI CC'TCA_GC
Cuts at: 0 1741 2364
Size: 1741 623
BfrBI ATG'CAT
Cuts at: 0 1477 2364
Size: 1477 887
Bme1580I G_kGCm'C
Cuts at: 0 104 671 769 2286 2364
Size: 104 567 98 1517 78
Fragments arranged by size:
1517 567 104 98 78
BmgBI CAC'GTC
Cuts at: 0 53 1571 1625 2364
Size: 53 1518 54 739
Fragments arranged by size:
1518 739 54 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 1042 1102 2364
Size: 1042 60 1262
Fragments arranged by size:
1262 1042 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 965 2246 2364
Size: 965 1281 118
Fragments arranged by size:
1281 965 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1690 2364
Size: 556 1134 674
Fragments arranged by size:
1134 674 556
Bpu10I CC'TnA_GC
Cuts at: 0 1741 2096 2195 2364
Size: 1741 355 99 169
Fragments arranged by size:
1741 355 169 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 937 979 2364
Size: 171 766 42 1385
Fragments arranged by size:
1385 766 171 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 133 1831 2364
Size: 133 1698 533
Fragments arranged by size:
1698 533 133
BsaHI Gr'CG_yC
Cuts at: 0 566 2364
Size: 566 1798
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1032 1062 2364
Size: 1032 30 1302
Fragments arranged by size:
1302 1032 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 136 746 946 954 1567 2140 2364
Size: 133 3 610 200 8 613 573 224
Fragments arranged by size:
613 610 573 224 200 133 8 3
BseYI C'CCAG_C
Cuts at: 0 670 924 1174 2349 2364
Size: 670 254 250 1175 15
Fragments arranged by size:
1175 670 254 250 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 2246 2364
Size: 55 2191 118
Fragments arranged by size:
2191 118 55
BsiHKAI G_wGCw'C
Cuts at: 0 946 1769 2097 2364
Size: 946 823 328 267
Fragments arranged by size:
946 823 328 267
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1112 1615 2364
Size: 1112 503 749
Fragments arranged by size:
1112 749 503
Bsp1286I G_dGCh'C
Cuts at: 0 104 671 769 946 1240 1723 1769 2097
Size: 104 567 98 177 294 483 46 328
Cuts at: 2097 2286 2364
Size: 189 78
Fragments arranged by size:
567 483 328 294 189 177 104 98
78 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1075 2364
Size: 1075 1289
BsrFI r'CCGG_y
Cuts at: 0 79 2364
Size: 79 2285
BssSI C'ACGA_G
Cuts at: 0 1115 2364
Size: 1115 1249
BstBI TT'CG_AA
Cuts at: 0 2202 2364
Size: 2202 162
BstEII G'GTnAC_C
Cuts at: 0 606 2364
Size: 606 1758
BstYI r'GATC_y
Cuts at: 0 20 1152 1278 1733 2364
Size: 20 1132 126 455 631
Fragments arranged by size:
1132 631 455 126 20
Bsu36I CC'TnA_GG
Cuts at: 0 712 2364
Size: 712 1652
BtgI C'CryG_G
Cuts at: 0 13 476 1274 1961 2364
Size: 13 463 798 687 403
Fragments arranged by size:
798 687 463 403 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 598 2364
Size: 598 1766
BtsI GCAGTG_nn'
Cuts at: 0 43 1317 1505 2364
Size: 43 1274 188 859
Fragments arranged by size:
1274 859 188 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1641 1676 2364
Size: 1641 35 688
Fragments arranged by size:
1641 688 35
DraI TTT'AAA
Cuts at: 0 1287 2364
Size: 1287 1077
EaeI y'GGCC_r
Cuts at: 0 1536 2364
Size: 1536 828
EarI CTCTTCn'nnn_
Cuts at: 0 800 1233 1778 1829 1936 2026 2156 2180
Size: 800 433 545 51 107 90 130 24
Cuts at: 2180 2364
Size: 184
Fragments arranged by size:
800 545 433 184 130 107 90 51
24
EcoICRI GAG'CTC
Cuts at: 0 944 2364
Size: 944 1420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 212 335 556 780 824 1690 2007
Size: 191 21 123 221 224 44 866 317
Cuts at: 2007 2364
Size: 357
Fragments arranged by size:
866 357 317 224 221 191 123 44
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 833 2364
Size: 833 1531
EcoO109I rG'GnC_Cy
Cuts at: 0 256 1689 1977 2006 2142 2282 2283 2364
Size: 256 1433 288 29 136 140 1 81
Fragments arranged by size:
1433 288 256 140 136 81 29 1
EcoRV GAT'ATC
Cuts at: 0 483 2364
Size: 483 1881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 373 405 1881 1913 1978 2010
Size: 130 32 211 32 1476 32 65 32
Cuts at: 2010 2364
Size: 354
Fragments arranged by size:
1476 354 211 130 65 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 955 1018 2364
Size: 955 63 1346
Fragments arranged by size:
1346 955 63
MfeI C'AATT_G
Cuts at: 0 725 2364
Size: 725 1639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 2364
Size: 791 1573
MscI TGG'CCA
Cuts at: 0 1538 2364
Size: 1538 826
MslI CAynn'nnrTG
Cuts at: 0 518 1343 2364
Size: 518 825 1021
Fragments arranged by size:
1021 825 518
MspA1I CmG'CkG
Cuts at: 0 1591 1762 2364
Size: 1591 171 602
Fragments arranged by size:
1591 602 171
NaeI GCC'GGC
Cuts at: 0 81 2364
Size: 81 2283
NcoI C'CATG_G
Cuts at: 0 1274 1961 2364
Size: 1274 687 403
Fragments arranged by size:
1274 687 403
NgoMIV G'CCGG_C
Cuts at: 0 79 2364
Size: 79 2285
NsiI A_TGCA'T
Cuts at: 0 1479 2364
Size: 1479 885
NspI r_CATG'y
Cuts at: 0 1481 2364
Size: 1481 883
PflMI CCAn_nnn'nTGG
Cuts at: 0 1635 2364
Size: 1635 729
PfoI T'CCnGG_A
Cuts at: 0 572 2364
Size: 572 1792
PpuMI rG'GwC_Cy
Cuts at: 0 256 2006 2364
Size: 256 1750 358
Fragments arranged by size:
1750 358 256
PspOMI G'GGCC_C
Cuts at: 0 100 2282 2364
Size: 100 2182 82
Fragments arranged by size:
2182 100 82
PspXI vC'TCGA_Gb
Cuts at: 0 613 2364
Size: 613 1751
PstI C_TGCA'G
Cuts at: 0 531 1211 1683 2291 2364
Size: 531 680 472 608 73
Fragments arranged by size:
680 608 531 472 73
PvuII CAG'CTG
Cuts at: 0 1591 1762 2364
Size: 1591 171 602
Fragments arranged by size:
1591 602 171
SacI G_AGCT'C
Cuts at: 0 946 2364
Size: 946 1418
SapI GCTCTTCn'nnn_
Cuts at: 0 1936 2364
Size: 1936 428
SfcI C'TryA_G
Cuts at: 0 527 1100 1207 1679 2287 2364
Size: 527 573 107 472 608 77
Fragments arranged by size:
608 573 527 472 107 77
SgrAI Cr'CCGG_yG
Cuts at: 0 79 2364
Size: 79 2285
SmlI C'TyrA_G
Cuts at: 0 150 613 952 958 2364
Size: 150 463 339 6 1406
Fragments arranged by size:
1406 463 339 150 6
StuI AGG'CCT
Cuts at: 0 619 2364
Size: 619 1745
StyI C'CwwG_G
Cuts at: 0 779 1012 1274 1961 2364
Size: 779 233 262 687 403
Fragments arranged by size:
779 687 403 262 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1377 2364
Size: 1377 987
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1032 1064 2364
Size: 1032 32 1300
Fragments arranged by size:
1300 1032 32
Tth111I GACn'n_nGTC
Cuts at: 0 54 2364
Size: 54 2310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 756 2364
Size: 756 1608
XhoI C'TCGA_G
Cuts at: 0 613 2364
Size: 613 1751
XmnI GAAnn'nnTTC
Cuts at: 0 1945 2364
Size: 1945 419
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BfrBI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I
HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NsiI NspI PflMI PfoI PpuMI PspOMI PspXI
PstI PvuII SacI SapI SfcI SgrAI SmlI StuI
StyI TaqII TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BclI BglI BglII
BlpI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstXI
BstZ17I ClaI DraIII DrdI EagI EciI EcoRI FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI NarI NdeI NheI NotI NruI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XmaI ZraI