(Circular) (Six-base) MAPSORT of: pFN21AB9571.seq Check: 63 from: 1 to: 5659 pFN21AB9571 5659 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2269 2269 Size: 5659 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2015 3002 1937 Size: 78 987 4594 Fragments arranged by size: 4594 987 78 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2427 3722 5106 5374 1632 Size: 795 1295 1384 268 1917 Fragments arranged by size: 1917 1384 1295 795 268 AflII C'TTAA_G Cuts at: 820 1017 2744 820 Size: 197 1727 3735 Fragments arranged by size: 3735 1727 197 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2388 2850 1184 Size: 1204 462 3993 Fragments arranged by size: 3993 1204 462 AlwNI CAG_nnn'CTG Cuts at: 2474 3402 4974 2474 Size: 928 1572 3159 Fragments arranged by size: 3159 1572 928 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2787 2968 3194 3346 5455 1493 Size: 1294 181 226 152 2109 1697 Fragments arranged by size: 2109 1697 1294 226 181 152 AseI AT'TA_AT Cuts at: 160 1032 2096 4213 160 Size: 872 1064 2117 1606 Fragments arranged by size: 2117 1606 1064 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2760 2984 1870 Size: 73 817 224 4545 Fragments arranged by size: 4545 817 224 73 BamHI G'GATC_C Cuts at: 2908 2989 3356 2908 Size: 81 367 5211 Fragments arranged by size: 5211 367 81 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2447 2826 2980 4435 618 Size: 325 217 627 660 379 154 1455 1842 Fragments arranged by size: 1842 1455 660 627 379 325 217 154 BanII G_rGCy'C Cuts at: 1391 2978 1391 Size: 1587 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4688 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2804 2838 3850 3884 1903 Size: 34 867 34 1012 34 3678 Fragments arranged by size: 3678 1012 867 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 2487 1548 Size: 939 4720 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2295 2510 4270 Size: 107 122 71 631 605 623 215 1760 Cuts at: 4270 136 Size: 1525 Fragments arranged by size: 1760 1525 631 623 605 215 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 3720 4876 1790 Size: 1930 1156 2573 Fragments arranged by size: 2573 1930 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 4348 442 Size: 416 53 83 399 24 1973 958 1753 Fragments arranged by size: 1973 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2441 2703 4319 1239 Size: 1202 262 1616 2579 Fragments arranged by size: 2579 1616 1202 262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3784 4649 4947 3784 Size: 865 298 4496 Fragments arranged by size: 4496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2716 3450 493 Size: 711 1512 734 2702 Fragments arranged by size: 2702 1512 734 711 BsaBI GATnn'nnATC Cuts at: 2255 2315 2492 5653 2255 Size: 60 177 3161 2261 Fragments arranged by size: 3161 2261 177 60 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2610 2640 1178 Size: 30 165 30 1207 30 4197 Fragments arranged by size: 4197 1207 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2764 1186 Size: 1578 4081 BseYI C'CCAG_C Cuts at: 2304 2497 4862 5358 2304 Size: 193 2365 496 2605 Fragments arranged by size: 2605 2365 496 193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2297 2303 1163 Size: 573 387 174 6 4519 Fragments arranged by size: 4519 573 387 174 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2521 3720 3805 4876 2521 Size: 1199 85 1071 3304 Fragments arranged by size: 3304 1199 1071 85 BsmI GAATG_Cn' Cuts at: 2725 3170 3263 2725 Size: 445 93 5121 Fragments arranged by size: 5121 445 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2521 2978 3720 3805 4876 1391 Size: 399 731 457 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 731 457 399 85 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 2628 3548 5278 2628 Size: 920 1730 3009 Fragments arranged by size: 3009 1730 920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2068 2095 2269 2820 3014 844 Size: 1224 27 174 551 194 3489 Fragments arranged by size: 3489 1224 551 194 174 27 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2608 2940 4154 4328 65 Size: 1183 1360 332 1214 174 1396 Fragments arranged by size: 1396 1360 1214 1183 332 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 2402 794 Size: 1608 4051 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2250 2256 2908 2989 3356 3741 3758 5210 Size: 278 6 652 81 367 385 17 1452 Cuts at: 5210 5296 5308 5654 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 652 385 367 346 278 86 81 17 12 6 BstZ17I GTA'TAC Cuts at: 2016 2016 Size: 5659 BtgI C'CryG_G Cuts at: 513 1065 2004 2838 513 Size: 552 939 834 3334 Fragments arranged by size: 3334 939 834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 2340 2566 3175 3969 3989 2340 Size: 226 609 794 20 4010 Fragments arranged by size: 4010 794 609 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2347 3106 Size: 54 1425 50 275 18 307 210 759 Cuts at: 3106 3995 5624 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 759 307 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2697 3589 1353 Size: 1344 892 3423 Fragments arranged by size: 3423 1344 892 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2427 2441 2703 3722 4319 5106 5374 Size: 393 795 14 262 1019 597 787 268 Cuts at: 5374 1239 Size: 1524 Fragments arranged by size: 1524 1019 795 787 597 393 268 262 14 EcoNI CCTnn'n_nnAGG Cuts at: 2478 2478 Size: 5659 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 4806 1678 Size: 3128 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2610 2642 4300 4332 4374 Size: 32 135 32 1038 32 1658 32 42 Cuts at: 4374 4406 1373 Size: 32 2626 Fragments arranged by size: 2626 1658 1038 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2139 2349 10 Size: 54 1425 343 307 210 3320 Fragments arranged by size: 3320 1425 343 307 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2832 2951 3617 3976 4135 518 Size: 648 1666 119 666 359 159 2042 Fragments arranged by size: 2042 1666 666 648 359 159 119 MspA1I CmG'CkG Cuts at: 1606 3075 3752 4900 5145 1606 Size: 1469 677 1148 245 2120 Fragments arranged by size: 2120 1469 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2838 513 Size: 552 939 834 3334 Fragments arranged by size: 3334 939 834 552 NdeI CA'TA_TG Cuts at: 387 2901 387 Size: 2514 3145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 3017 4562 1132 Size: 97 1788 1545 2229 Fragments arranged by size: 2229 1788 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2336 2420 1613 Size: 723 84 4852 Fragments arranged by size: 4852 723 84 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 3011 830 Size: 2181 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SapI GCTCTTCn'nnn_ Cuts at: 2697 2697 Size: 5659 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 3007 4142 4823 5014 826 Size: 219 1962 1135 681 191 1471 Fragments arranged by size: 1962 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2295 2295 Size: 5659 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 3763 4664 4926 820 Size: 197 926 801 1019 901 262 1553 Fragments arranged by size: 1553 1019 926 901 801 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2004 2083 2838 3098 513 Size: 552 939 79 755 260 3074 Fragments arranged by size: 3074 939 755 552 260 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3686 3703 3856 4041 4486 325 Size: 1255 2106 17 153 185 445 1498 Fragments arranged by size: 2106 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3905 5617 96 Size: 274 80 33 51 1443 1928 1712 138 Fragments arranged by size: 1928 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2259 2291 2376 2408 2839 2871 2931 2963 5619 Size: 32 85 32 431 32 60 32 2656 Cuts at: 5619 5651 2259 Size: 32 2267 Fragments arranged by size: 2656 2267 431 85 60 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2175 1316 Size: 859 4800 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2162 2261 2162 Size: 99 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI