(Circular) (Six-base) MAPSORT of: pFN21AB9564.seq Check: 3335 from: 1 to: 5434 pFN21AB9564 5434 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3244 278 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2427 2777 1937 Size: 490 350 4594 Fragments arranged by size: 4594 490 350 Acc65I G'GTAC_C Cuts at: 2755 2755 Size: 5434 AclI AA'CG_TT Cuts at: 1563 3561 3934 1563 Size: 1998 373 3063 Fragments arranged by size: 3063 1998 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 2513 3497 4881 5149 1632 Size: 755 126 984 1384 268 1917 Fragments arranged by size: 1917 1384 984 755 268 126 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5237 AflIII A'CryG_T Cuts at: 1203 4333 5310 1203 Size: 3130 977 1327 Fragments arranged by size: 3130 1327 977 AgeI A'CCGG_T Cuts at: 4265 4265 Size: 5434 AhdI GACnn_n'nnGTC Cuts at: 4163 4163 Size: 5434 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5434 AlwNI CAG_nnn'CTG Cuts at: 2323 2365 3177 4749 2323 Size: 42 812 1572 3008 Fragments arranged by size: 3008 1572 812 42 ApaI G_GGCC'C Cuts at: 2419 2563 2635 2716 2419 Size: 144 72 81 5137 Fragments arranged by size: 5137 144 81 72 ApaLI G'TGCA_C Cuts at: 3491 4647 3491 Size: 1156 4278 ApoI r'AATT_y Cuts at: 1493 2743 2969 3121 5230 1493 Size: 1250 226 152 2109 1697 Fragments arranged by size: 2109 1697 1250 226 152 AseI AT'TA_AT Cuts at: 160 1032 3988 160 Size: 872 2956 1606 Fragments arranged by size: 2956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 AvaI C'yCGr_G Cuts at: 1870 1943 2556 2619 2639 2759 1870 Size: 73 613 63 20 120 4545 Fragments arranged by size: 4545 613 120 73 63 20 BamHI G'GATC_C Cuts at: 2478 2764 3131 2478 Size: 286 367 4781 Fragments arranged by size: 4781 367 286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2539 2688 2755 4210 618 Size: 325 217 627 752 149 67 1455 1842 Fragments arranged by size: 1842 1455 752 627 325 217 149 67 BanII G_rGCy'C Cuts at: 1391 2419 2563 2635 2716 2753 1391 Size: 1028 144 72 81 37 4072 Fragments arranged by size: 4072 1028 144 81 72 37 BbeI G_GCGC'C Cuts at: 2692 2692 Size: 5434 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2235 928 Size: 971 336 4127 Fragments arranged by size: 4127 971 336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3625 3659 1903 Size: 34 1688 34 3678 Fragments arranged by size: 3678 1688 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3326 4536 3326 Size: 1210 4224 BclI T'GATC_A Cuts at: 1548 1548 Size: 5434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2630 2711 4045 Size: 107 122 71 631 605 958 81 1334 Cuts at: 4045 136 Size: 1525 Fragments arranged by size: 1525 1334 958 631 605 122 107 81 71 BglII A'GATC_T Cuts at: 5429 5429 Size: 5434 BlpI GC'TnA_GC Cuts at: 2372 2600 2851 2372 Size: 228 251 4955 Fragments arranged by size: 4955 251 228 Bme1580I G_kGCm'C Cuts at: 1790 2419 2563 2635 2716 3495 4651 1790 Size: 629 144 72 81 779 1156 2573 Fragments arranged by size: 2573 1156 779 629 144 81 72 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 2147 3165 Size: 416 53 83 399 24 681 49 1018 Cuts at: 3165 4123 442 Size: 958 1753 Fragments arranged by size: 1753 1018 958 681 416 399 83 53 49 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5434 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 4094 1239 Size: 760 2095 2579 Fragments arranged by size: 2579 2095 760 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3559 4424 4722 3559 Size: 865 298 4271 Fragments arranged by size: 4271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 4097 882 Size: 617 714 1884 2219 Fragments arranged by size: 2219 1884 714 617 BsaAI yAC'GTr Cuts at: 493 1204 3225 493 Size: 711 2021 2702 Fragments arranged by size: 2702 2021 711 BsaBI GATnn'nnATC Cuts at: 5428 5428 Size: 5434 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2689 3241 3623 4258 Size: 53 83 186 936 1156 552 382 635 Cuts at: 4258 275 Size: 1451 Fragments arranged by size: 1451 1156 936 635 552 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3867 4265 4539 4686 5097 3867 Size: 398 274 147 411 4204 Fragments arranged by size: 4204 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5209 Fragments arranged by size: 5209 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2438 2690 2741 2744 1186 Size: 889 363 252 51 3 3876 Fragments arranged by size: 3876 889 363 252 51 3 BseYI C'CCAG_C Cuts at: 2470 4637 5133 2470 Size: 2167 496 2771 Fragments arranged by size: 2771 2167 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4861 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2884 3645 4673 1540 Size: 275 186 883 761 1028 2301 Fragments arranged by size: 2301 1028 883 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3495 3580 4651 3495 Size: 85 1071 4278 Fragments arranged by size: 4278 1071 85 BsmI GAATG_Cn' Cuts at: 2332 2945 3038 2332 Size: 613 93 4728 Fragments arranged by size: 4728 613 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2419 2563 2635 2716 2753 3495 3580 Size: 399 629 144 72 81 37 742 85 Cuts at: 3580 4651 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 629 399 144 85 81 72 37 BspEI T'CCGG_A Cuts at: 5097 5097 Size: 5434 BspHI T'CATG_A Cuts at: 3323 5053 3323 Size: 1730 3704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 844 Size: 1945 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2702 3321 1394 Size: 429 879 619 3507 Fragments arranged by size: 3507 879 619 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3929 4103 65 Size: 1183 2681 174 1396 Fragments arranged by size: 2681 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2513 2604 4078 4265 1700 Size: 110 703 91 1474 187 2869 Fragments arranged by size: 2869 1474 703 187 110 91 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5434 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5434 BssSI C'ACGA_G Cuts at: 3494 4506 3494 Size: 1012 4422 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5434 BstBI TT'CG_AA Cuts at: 4238 4238 Size: 5434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5434 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2230 2284 2062 Size: 168 54 5212 Fragments arranged by size: 5212 168 54 BstYI r'GATC_y Cuts at: 1972 2478 2764 3131 3516 3533 4985 5071 5083 Size: 506 286 367 385 17 1452 86 12 Cuts at: 5083 5429 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 506 385 367 346 286 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2030 513 Size: 552 939 26 3917 Fragments arranged by size: 3917 939 552 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4695 Fragments arranged by size: 4695 683 56 BtsI GCAGTG_nn' Cuts at: 2725 2950 3744 3764 2725 Size: 225 794 20 4395 Fragments arranged by size: 4395 794 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2240 2275 529 Size: 35 1676 35 3688 Fragments arranged by size: 3688 1676 35 35 DraI TTT'AAA Cuts at: 2738 2738 Size: 5434 DraIII CAC_nnn'GTG Cuts at: 2233 2233 Size: 5434 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4441 809 Size: 507 3125 1802 Fragments arranged by size: 3125 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2380 2511 2703 Size: 54 1425 50 275 18 550 131 192 Cuts at: 2703 2881 3770 5399 8 Size: 178 889 1629 43 Fragments arranged by size: 1629 1425 889 550 275 192 178 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2881 1537 Size: 275 1069 4090 Fragments arranged by size: 4090 1069 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 3364 1353 Size: 782 1229 3423 Fragments arranged by size: 3423 1229 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2491 4021 4395 4541 387 Size: 1064 138 902 1530 374 146 1280 Fragments arranged by size: 1530 1280 1064 902 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2387 2513 3497 4094 4881 5149 Size: 393 367 388 126 984 597 787 268 Cuts at: 5149 1239 Size: 1524 Fragments arranged by size: 1524 984 787 597 393 388 367 268 126 EcoNI CCTnn'n_nnAGG Cuts at: 2357 2357 Size: 5434 EcoO109I rG'GnC_Cy Cuts at: 2362 2415 2416 2488 2560 2631 2712 2362 Size: 53 1 72 72 71 81 5084 Fragments arranged by size: 5084 81 72 72 71 53 1 EcoRI G'AATT_C Cuts at: 2743 3121 2743 Size: 378 5056 FspI TGC'GCA Cuts at: 3940 3940 Size: 5434 HaeII r_GCGC'y Cuts at: 1678 2692 4581 1678 Size: 1014 1889 2531 Fragments arranged by size: 2531 1889 1014 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2231 2263 4075 4107 4149 Size: 32 135 32 659 32 1812 32 42 Cuts at: 4149 4181 1373 Size: 32 2626 Fragments arranged by size: 2626 1812 659 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2778 3024 669 Size: 988 281 840 246 3079 Fragments arranged by size: 3079 988 840 281 246 HindIII A'AGCT_T Cuts at: 748 2495 748 Size: 1747 3687 HpaI GTT'AAC Cuts at: 3024 3024 Size: 5434 KasI G'GCGC_C Cuts at: 2688 2688 Size: 5434 KpnI G_GTAC'C Cuts at: 2759 2759 Size: 5434 MfeI C'AATT_G Cuts at: 3033 3033 Size: 5434 MluI A'CGCG_T Cuts at: 5310 5310 Size: 5434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4548 4732 1305 Size: 3243 184 2007 Fragments arranged by size: 3243 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2382 10 Size: 54 1425 343 550 3062 Fragments arranged by size: 3062 1425 550 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2282 3392 3751 3910 518 Size: 648 894 222 1110 359 159 2042 Fragments arranged by size: 2042 1110 894 648 359 222 159 MspA1I CmG'CkG Cuts at: 1606 2320 2850 3527 4675 4920 1606 Size: 714 530 677 1148 245 2120 Fragments arranged by size: 2120 1148 714 677 530 245 NaeI GCC'GGC Cuts at: 2515 2606 2515 Size: 91 5343 NarI GG'CG_CC Cuts at: 2689 2689 Size: 5434 NcoI C'CATG_G Cuts at: 513 1065 2004 2030 513 Size: 552 939 26 3917 Fragments arranged by size: 3917 939 552 26 NdeI CA'TA_TG Cuts at: 387 387 Size: 5434 NgoMIV G'CCGG_C Cuts at: 2513 2604 2513 Size: 91 5343 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5434 NotI GC'GGCC_GC Cuts at: 2881 2881 Size: 5434 NspI r_CATG'y Cuts at: 1132 1229 2045 2792 4337 1132 Size: 97 816 747 1545 2229 Fragments arranged by size: 2229 1545 816 747 97 PasI CC'CwG_GG Cuts at: 1348 2359 1348 Size: 1011 4423 PciI A'CATG_T Cuts at: 4333 4333 Size: 5434 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5434 PmeI GTTT'AAAC Cuts at: 2738 2738 Size: 5434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3535 5016 5048 3503 Size: 32 1481 32 3889 Fragments arranged by size: 3889 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5434 PsiI TTA'TAA Cuts at: 3004 3004 Size: 5434 PspOMI G'GGCC_C Cuts at: 2415 2559 2631 2712 2415 Size: 144 72 81 5137 Fragments arranged by size: 5137 144 81 72 PspXI vC'TCGA_Gb Cuts at: 2639 2639 Size: 5434 PstI C_TGCA'G Cuts at: 830 2786 830 Size: 1956 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5434 PvuII CAG'CTG Cuts at: 2320 2320 Size: 5434 SalI G'TCGA_C Cuts at: 1936 2776 1936 Size: 840 4594 SbfI CC_TGCA'GG Cuts at: 2786 2786 Size: 5434 ScaI AGT'ACT Cuts at: 3682 5394 3682 Size: 1712 3722 SexAI A'CCwGG_T Cuts at: 2229 2229 Size: 5434 SfcI C'TryA_G Cuts at: 826 1045 2782 3917 4598 4789 826 Size: 219 1737 1135 681 191 1471 Fragments arranged by size: 1737 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2711 2711 Size: 5434 SfoI GGC'GCC Cuts at: 2690 2690 Size: 5434 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5434 SmaI CCC'GGG Cuts at: 1872 2558 2761 1872 Size: 686 203 4545 Fragments arranged by size: 4545 686 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 3538 4439 4701 820 Size: 197 926 696 899 901 262 1553 Fragments arranged by size: 1553 926 901 899 696 262 197 SnaBI TAC'GTA Cuts at: 493 3225 493 Size: 2732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5434 SphI G_CATG'C Cuts at: 1132 2792 1132 Size: 1660 3774 SspI AAT'ATT Cuts at: 5 52 3358 5 Size: 47 3306 2081 Fragments arranged by size: 3306 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5434 StyI C'CwwG_G Cuts at: 513 1065 2004 2030 2331 2646 2706 2873 513 Size: 552 939 26 301 315 60 167 3074 Fragments arranged by size: 3074 939 552 315 301 167 60 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3461 3478 3631 3816 4261 325 Size: 1255 1881 17 153 185 445 1498 Fragments arranged by size: 1881 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3680 5392 96 Size: 274 80 33 51 1443 1703 1712 138 Fragments arranged by size: 1712 1703 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2028 2060 5394 5426 2028 Size: 32 3334 32 2036 Fragments arranged by size: 3334 2036 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5434 XbaI T'CTAG_A Cuts at: 2770 2770 Size: 5434 XhoI C'TCGA_G Cuts at: 1943 2639 1943 Size: 696 4738 XmaI C'CCGG_G Cuts at: 1870 2556 2759 1870 Size: 686 203 4545 Fragments arranged by size: 4545 686 203 XmnI GAAnn'nnTTC Cuts at: 3563 3563 Size: 5434 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3242 276 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI SrfI SwaI XcmI