(Circular) (Six-base) MAPSORT of: pFN21AB9563.seq Check: 8448 from: 1 to: 11671 pFN21AB9563 11671 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2790 4145 4285 5311 5824 6181 8147 8408 2790 Size: 1355 140 1026 513 357 1966 261 6053 Fragments arranged by size: 6053 1966 1355 1026 513 357 261 140 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8775 9481 278 Size: 53 83 186 936 7239 706 2468 Fragments arranged by size: 7239 2468 936 706 186 83 53 AccI GT'mk_AC Cuts at: 1937 4917 6534 6957 9014 1937 Size: 2980 1617 423 2057 4594 Fragments arranged by size: 4594 2980 2057 1617 423 Acc65I G'GTAC_C Cuts at: 2810 4230 8992 2810 Size: 1420 4762 5489 Fragments arranged by size: 5489 4762 1420 AclI AA'CG_TT Cuts at: 1563 9798 10171 1563 Size: 8235 373 3063 Fragments arranged by size: 8235 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2604 3986 5119 5741 6082 6776 7987 8059 Size: 972 1382 1133 622 341 694 1211 72 Cuts at: 8059 9734 11118 11386 1632 Size: 1675 1384 268 1917 Fragments arranged by size: 1917 1675 1384 1382 1211 1133 972 694 622 341 268 72 AfeI AGC'GCT Cuts at: 7229 7229 Size: 11671 AflII C'TTAA_G Cuts at: 820 1017 8948 820 Size: 197 7931 3543 Fragments arranged by size: 7931 3543 197 AflIII A'CryG_T Cuts at: 1203 8667 10570 11547 1203 Size: 7464 1903 977 1327 Fragments arranged by size: 7464 1903 1327 977 AgeI A'CCGG_T Cuts at: 10502 10502 Size: 11671 AhdI GACnn_n'nnGTC Cuts at: 5534 6517 10400 5534 Size: 983 3883 6805 Fragments arranged by size: 6805 3883 983 AleI CACnn'nnGTG Cuts at: 1166 2339 2829 3897 4683 4871 5447 6593 7371 Size: 1173 490 1068 786 188 576 1146 778 Cuts at: 7371 7404 8867 1166 Size: 33 1463 3970 Fragments arranged by size: 3970 1463 1173 1146 1068 786 778 576 490 188 33 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3157 4031 5688 6611 1184 Size: 1973 874 1657 923 6244 Fragments arranged by size: 6244 1973 1657 923 874 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3905 3937 5367 5399 3905 Size: 32 1430 32 10177 Fragments arranged by size: 10177 1430 32 32 AlwNI CAG_nnn'CTG Cuts at: 3298 3409 5297 5578 6196 6772 6842 6856 7598 Size: 111 1888 281 618 576 70 14 742 Cuts at: 7598 8782 9414 10986 3298 Size: 1184 632 1572 3983 Fragments arranged by size: 3983 1888 1572 1184 742 632 618 576 281 111 70 14 ApaI G_GGCC'C Cuts at: 2631 4123 5708 6159 6881 7567 7674 8407 8886 Size: 1492 1585 451 722 686 107 733 479 Cuts at: 8886 2631 Size: 5416 Fragments arranged by size: 5416 1585 1492 733 722 686 479 451 107 ApaLI G'TGCA_C Cuts at: 5285 5663 9728 10884 5285 Size: 378 4065 1156 6072 Fragments arranged by size: 6072 4065 1156 378 ApoI r'AATT_y Cuts at: 1493 8980 9206 9358 11467 1493 Size: 7487 226 152 2109 1697 Fragments arranged by size: 7487 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 10225 160 Size: 872 9193 1606 Fragments arranged by size: 9193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11671 AvaI C'yCGr_G Cuts at: 1870 1943 2975 3423 3446 3460 3582 5094 5149 Size: 73 1032 448 23 14 122 1512 55 Cuts at: 5149 6035 6995 7053 7151 7536 7560 8298 8315 Size: 886 960 58 98 385 24 738 17 Cuts at: 8315 8540 8996 1870 Size: 225 456 4545 Fragments arranged by size: 4545 1512 1032 960 886 738 456 448 385 225 122 98 73 58 55 24 23 17 14 BamHI G'GATC_C Cuts at: 5310 9001 9368 5310 Size: 3691 367 7613 Fragments arranged by size: 7613 3691 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2713 2810 3586 3791 Size: 325 217 627 335 591 97 776 205 Cuts at: 3791 4230 4729 5432 5882 6580 7379 7627 7846 Size: 439 499 703 450 698 799 248 219 Cuts at: 7846 7858 8177 8992 10447 618 Size: 12 319 815 1455 1842 Fragments arranged by size: 1842 1455 815 799 776 703 698 627 591 499 450 439 335 325 319 248 219 217 205 97 12 BanII G_rGCy'C Cuts at: 1391 2419 2442 2631 3447 3469 4123 5226 5708 Size: 1028 23 189 816 22 654 1103 482 Cuts at: 5708 5846 6159 6220 6633 6881 7002 7032 7302 Size: 138 313 61 413 248 121 30 270 Cuts at: 7302 7567 7674 8140 8316 8407 8625 8886 8990 Size: 265 107 466 176 91 218 261 104 Cuts at: 8990 1391 Size: 4072 Fragments arranged by size: 4072 1103 1028 816 654 482 466 413 313 270 265 261 248 218 189 176 138 121 107 104 91 61 30 23 22 BbeI G_GCGC'C Cuts at: 5886 6584 5886 Size: 698 10973 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3078 4899 5014 5743 928 Size: 971 1179 1821 115 729 6856 Fragments arranged by size: 6856 1821 1179 971 729 115 BbvCI CC'TCA_GC Cuts at: 2285 2401 2942 5618 6182 6254 7503 7646 2285 Size: 116 541 2676 564 72 1249 143 6310 Fragments arranged by size: 6310 2676 1249 564 541 143 116 72 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 9862 9896 1903 Size: 34 50 34 7841 34 3678 Fragments arranged by size: 7841 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9563 10773 9563 Size: 1210 10461 BclI T'GATC_A Cuts at: 1548 1548 Size: 11671 BfrBI ATG'CAT Cuts at: 8108 8108 Size: 11671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2729 3215 3724 Size: 107 122 71 631 605 1057 486 509 Cuts at: 3724 4190 4604 5230 5610 5804 7439 7724 10282 Size: 466 414 626 380 194 1635 285 2558 Cuts at: 10282 136 Size: 1525 Fragments arranged by size: 2558 1635 1525 1057 631 626 605 509 486 466 414 380 285 194 122 107 71 BglII A'GATC_T Cuts at: 5742 11666 5742 Size: 5924 5747 BlpI GC'TnA_GC Cuts at: 3387 7760 9088 3387 Size: 4373 1328 5970 Fragments arranged by size: 5970 4373 1328 Bme1580I G_kGCm'C Cuts at: 1790 2456 2631 2663 3335 3433 3591 4123 4520 Size: 666 175 32 672 98 158 532 397 Cuts at: 4520 5289 5435 5667 5708 6159 6590 6881 7382 Size: 769 146 232 41 451 431 291 501 Cuts at: 7382 7411 7552 7567 7630 7674 7849 7863 8298 Size: 29 141 15 63 44 175 14 435 Cuts at: 8298 8407 8449 8886 9732 10888 1790 Size: 109 42 437 846 1156 2573 Fragments arranged by size: 2573 1156 846 769 672 666 532 501 451 437 435 431 397 291 232 175 175 158 146 141 109 98 63 44 42 41 32 29 15 14 BmgBI CAC'GTC Cuts at: 1318 2175 3499 5128 5506 7060 1318 Size: 857 1324 1629 378 1554 5929 Fragments arranged by size: 5929 1629 1554 1324 857 378 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2466 4125 4325 Size: 416 53 83 399 24 1049 1659 200 Cuts at: 4325 7954 8974 9402 10360 442 Size: 3629 1020 428 958 1753 Fragments arranged by size: 3629 1753 1659 1049 1020 958 428 416 399 200 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11671 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2957 3562 2957 Size: 605 11066 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2283 2972 2993 3170 3584 4307 4391 Size: 899 145 689 21 177 414 723 84 Cuts at: 4391 4445 4457 5378 5386 5810 6007 6077 6218 Size: 54 12 921 8 424 197 70 141 Cuts at: 6218 6289 6814 7016 7400 7874 8282 8399 8456 Size: 71 525 202 384 474 408 117 57 Cuts at: 8456 8874 10331 1239 Size: 418 1457 2579 Fragments arranged by size: 2579 1457 921 899 723 689 525 474 424 418 414 408 384 202 197 177 145 141 117 84 71 70 57 54 21 12 8 Bpu10I CC'TnA_GC Cuts at: 2285 2401 2942 3240 3255 4235 4313 5618 6182 Size: 116 541 298 15 980 78 1305 564 Cuts at: 6182 6254 7503 7646 8619 8648 2285 Size: 72 1249 143 973 29 5308 Fragments arranged by size: 5308 1305 1249 980 973 564 541 298 143 116 78 72 29 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3365 5403 6225 8378 8678 9796 10661 10959 3365 Size: 2038 822 2153 300 1118 865 298 4077 Fragments arranged by size: 4077 2153 2038 1118 865 822 300 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3816 4064 4397 4931 7145 8816 10334 Size: 617 2317 248 333 534 2214 1671 1518 Cuts at: 10334 882 Size: 2219 Fragments arranged by size: 2317 2219 2214 1671 1518 617 534 333 248 BsaAI yAC'GTr Cuts at: 493 1204 4595 6301 9462 493 Size: 711 3391 1706 3161 2702 Fragments arranged by size: 3391 3161 2702 1706 711 BsaBI GATnn'nnATC Cuts at: 11665 11665 Size: 11671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2248 5163 5883 6042 Size: 53 83 186 936 715 2915 720 159 Cuts at: 6042 6581 8454 8772 9478 9860 10495 275 Size: 539 1873 318 706 382 635 1451 Fragments arranged by size: 2915 1873 1451 936 720 715 706 635 539 382 318 186 159 83 53 BsaWI w'CCGG_w Cuts at: 10104 10502 10776 10923 11334 10104 Size: 398 274 147 411 10441 Fragments arranged by size: 10441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3146 3176 5367 5397 5954 Size: 30 165 30 1743 30 2191 30 557 Cuts at: 5954 5984 7595 7625 8042 8072 8691 8721 1178 Size: 30 1611 30 417 30 619 30 4128 Fragments arranged by size: 4128 2191 1743 1611 619 557 417 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2294 2389 2534 2949 3167 3170 3173 3787 Size: 1108 95 145 415 218 3 3 614 Cuts at: 3787 4454 4712 5021 5183 6511 6737 7310 7870 Size: 667 258 309 162 1328 226 573 560 Cuts at: 7870 7937 8047 8050 1186 Size: 67 110 3 4807 Fragments arranged by size: 4807 1328 1108 667 614 573 560 415 309 258 226 218 162 145 110 95 67 3 3 3 BseYI C'CCAG_C Cuts at: 2178 2212 2361 3058 3291 3721 4395 5166 5523 Size: 34 149 697 233 430 674 771 357 Cuts at: 5523 5568 5903 6207 6551 6615 6849 6882 6906 Size: 45 335 304 344 64 234 33 24 Cuts at: 6906 6962 7107 7115 7224 7268 7310 7335 7383 Size: 56 145 8 109 44 42 25 48 Cuts at: 7383 7566 8371 10874 11370 2178 Size: 183 805 2503 496 2479 Fragments arranged by size: 2503 2479 805 771 697 674 496 430 357 344 335 304 234 233 183 149 145 109 64 56 48 45 44 42 34 33 25 24 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3380 3891 4313 4364 5084 5384 5939 Size: 573 1644 511 422 51 720 300 555 Cuts at: 5939 5951 7856 1163 Size: 12 1905 4978 Fragments arranged by size: 4978 1905 1644 720 573 555 511 422 300 51 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3846 3986 5448 9121 9882 10910 Size: 275 186 1845 140 1462 3673 761 1028 Cuts at: 10910 1540 Size: 2301 Fragments arranged by size: 3673 2301 1845 1462 1028 761 275 186 140 BsiHKAI G_wGCw'C Cuts at: 3447 4241 5289 5454 5667 6031 6503 6699 6747 Size: 794 1048 165 213 364 472 196 48 Cuts at: 6747 7032 7248 9732 9817 10888 3447 Size: 285 216 2484 85 1071 4230 Fragments arranged by size: 4230 2484 1071 1048 794 472 364 285 216 213 196 165 85 48 BsmI GAATG_Cn' Cuts at: 9182 9275 9182 Size: 93 11578 BsmBI CGTCTCn'nnnn_ Cuts at: 2300 3088 2300 Size: 788 10883 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2419 2442 2456 2631 2663 3335 3433 Size: 399 629 23 14 175 32 672 98 Cuts at: 3433 3447 3469 3591 4123 4241 4520 5226 5289 Size: 14 22 122 532 118 279 706 63 Cuts at: 5289 5435 5454 5667 5708 5846 6031 6159 6220 Size: 146 19 213 41 138 185 128 61 Cuts at: 6220 6503 6590 6633 6699 6747 6881 7002 7032 Size: 283 87 43 66 48 134 121 30 Cuts at: 7032 7248 7302 7382 7411 7552 7567 7630 7674 Size: 216 54 80 29 141 15 63 44 Cuts at: 7674 7849 7863 8140 8298 8316 8407 8449 8625 Size: 175 14 277 158 18 91 42 176 Cuts at: 8625 8886 8990 9732 9817 10888 1391 Size: 261 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 706 672 629 532 399 283 279 277 261 216 213 185 176 175 175 158 146 141 138 134 128 122 121 118 104 98 91 87 85 80 66 63 63 61 54 48 44 43 42 41 32 30 29 23 22 19 18 15 14 14 14 BspEI T'CCGG_A Cuts at: 11334 11334 Size: 11671 BspHI T'CATG_A Cuts at: 8065 9560 11290 8065 Size: 1495 1730 8446 Fragments arranged by size: 8446 1730 1495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 2245 2790 4145 4285 5311 5586 5824 Size: 1319 82 545 1355 140 1026 275 238 Cuts at: 5824 5973 6181 8147 8408 9026 844 Size: 149 208 1966 261 618 3489 Fragments arranged by size: 3489 1966 1355 1319 1026 618 545 275 261 238 208 149 140 82 BsrBI CCG'CTC Cuts at: 1394 1823 4819 7495 8860 9558 1394 Size: 429 2996 2676 1365 698 3507 Fragments arranged by size: 3507 2996 2676 1365 698 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5691 10166 10340 65 Size: 1183 4443 4475 174 1396 Fragments arranged by size: 4475 4443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2706 2739 2886 3137 3838 3872 3985 Size: 110 896 33 147 251 701 34 113 Cuts at: 3985 4908 5855 6891 10315 10502 1700 Size: 923 947 1036 3424 187 2869 Fragments arranged by size: 3424 2869 1036 947 923 896 701 251 187 147 113 110 34 33 BsrGI T'GTAC_A Cuts at: 96 96 Size: 11671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11671 BssSI C'ACGA_G Cuts at: 4724 6632 7545 9731 10743 4724 Size: 1908 913 2186 1012 5652 Fragments arranged by size: 5652 2186 1908 1012 913 BstAPI GCAn_nnn'nTGC Cuts at: 794 5578 6988 7243 794 Size: 4784 1410 255 5222 Fragments arranged by size: 5222 4784 1410 255 BstBI TT'CG_AA Cuts at: 2927 10475 2927 Size: 7548 4123 BstEII G'GTnAC_C Cuts at: 1184 4573 4688 1184 Size: 3389 115 8167 Fragments arranged by size: 8167 3389 115 BstXI CCAn_nnnn'nTGG Cuts at: 3788 3899 4081 4685 5410 6175 7204 7373 7391 Size: 111 182 604 725 765 1029 169 18 Cuts at: 7391 7801 8695 3788 Size: 410 894 6764 Fragments arranged by size: 6764 1029 894 765 725 604 410 182 169 111 18 BstYI r'GATC_y Cuts at: 1972 3996 4308 5079 5310 5742 6526 9001 9368 Size: 2024 312 771 231 432 784 2475 367 Cuts at: 9368 9753 9770 11222 11308 11320 11666 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2475 2024 1977 1452 784 771 432 385 367 346 312 231 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2480 2501 3213 3729 4734 5261 5979 6016 6108 Size: 21 712 516 1005 527 718 37 92 Cuts at: 6108 6818 2480 Size: 710 7333 Fragments arranged by size: 7333 1005 718 712 710 527 516 92 37 21 BtgI C'CryG_G Cuts at: 513 1065 2018 2088 2133 2790 2901 2957 3228 Size: 552 953 70 45 657 111 56 271 Cuts at: 3228 3436 3810 3895 4080 4269 4828 5302 5634 Size: 208 374 85 185 189 559 474 332 Cuts at: 5634 5646 7176 7366 7437 8165 8604 513 Size: 12 1530 190 71 728 439 3580 Fragments arranged by size: 3580 1530 953 728 657 559 552 474 439 374 332 271 208 190 189 185 111 85 71 70 56 45 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3948 5154 6006 487 Size: 683 56 2722 1206 852 6152 Fragments arranged by size: 6152 2722 1206 852 683 56 BtsI GCAGTG_nn' Cuts at: 4376 4993 5346 5488 5814 5946 6137 6468 8245 Size: 617 353 142 326 132 191 331 1777 Cuts at: 8245 9187 9981 10001 4376 Size: 942 794 20 6046 Fragments arranged by size: 6046 1777 942 794 617 353 331 326 191 142 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11636 DraI TTT'AAA Cuts at: 8975 8975 Size: 11671 DraIII CAC_nnn'GTG Cuts at: 2338 4356 5852 2338 Size: 2018 1496 8157 Fragments arranged by size: 8157 2018 1496 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3080 6335 8201 8777 10678 809 Size: 507 1764 3255 1866 576 1901 1802 Fragments arranged by size: 3255 1901 1866 1802 1764 576 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2130 2533 Size: 54 1425 50 275 18 243 57 403 Cuts at: 2533 2841 3088 3221 3321 3957 3975 3983 3987 Size: 308 247 133 100 636 18 8 4 Cuts at: 3987 4534 4567 4906 5031 5211 5391 5445 7062 Size: 547 33 339 125 180 180 54 1617 Cuts at: 7062 7206 7725 8223 8278 8631 8875 8956 9118 Size: 144 519 498 55 353 244 81 162 Cuts at: 9118 10007 11636 8 Size: 889 1629 43 Fragments arranged by size: 1629 1617 1425 889 636 547 519 498 403 353 339 308 275 247 244 243 180 180 162 144 133 125 100 81 57 55 54 54 50 43 33 18 18 8 4 EagI C'GGCC_G Cuts at: 1537 1812 3983 5445 9118 1537 Size: 275 2171 1462 3673 4090 Fragments arranged by size: 4090 3673 2171 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2580 5261 5834 6276 7253 8738 9601 1353 Size: 1227 2681 573 442 977 1485 863 3423 Fragments arranged by size: 3423 2681 1485 1227 977 863 573 442 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6668 7813 8243 10258 10632 10778 Size: 1064 138 5079 1145 430 2015 374 146 Cuts at: 10778 387 Size: 1280 Fragments arranged by size: 5079 2015 1280 1145 1064 430 374 146 138 EcoICRI GAG'CTC Cuts at: 3445 7030 3445 Size: 3585 8086 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2283 2604 2972 2993 3170 3584 Size: 393 506 145 321 368 21 177 414 Cuts at: 3584 3986 4307 4391 4445 4457 5119 5378 5386 Size: 402 321 84 54 12 662 259 8 Cuts at: 5386 5741 5810 6007 6077 6082 6218 6289 6776 Size: 355 69 197 70 5 136 71 487 Cuts at: 6776 6814 7016 7400 7874 7987 8059 8282 8399 Size: 38 202 384 474 113 72 223 117 Cuts at: 8399 8456 8874 9734 10331 11118 11386 1239 Size: 57 418 860 597 787 268 1524 Fragments arranged by size: 1524 860 787 662 597 506 487 474 418 414 402 393 384 368 355 321 321 268 259 223 202 197 177 145 136 117 113 84 72 71 70 69 57 54 38 21 12 8 5 EcoNI CCTnn'n_nnAGG Cuts at: 2479 3258 3380 4439 4805 5421 6224 7949 8261 Size: 779 122 1059 366 616 803 1725 312 Cuts at: 8261 8892 2479 Size: 631 5258 Fragments arranged by size: 5258 1725 1059 803 779 631 616 366 312 122 EcoO109I rG'GnC_Cy Cuts at: 2428 2460 2628 3725 3769 3784 4119 4120 5188 Size: 32 168 1097 44 15 335 1 1068 Cuts at: 5188 5257 5575 5704 5956 6156 6249 6877 6878 Size: 69 318 129 252 200 93 628 1 Cuts at: 6878 6928 7147 7431 7670 7955 8267 8353 8403 Size: 50 219 284 239 285 312 86 50 Cuts at: 8403 8404 8600 8779 8882 8883 2428 Size: 1 196 179 103 1 5216 Fragments arranged by size: 5216 1097 1068 628 335 318 312 285 284 252 239 219 200 196 179 168 129 103 93 86 69 50 50 44 32 15 1 1 1 1 EcoRI G'AATT_C Cuts at: 8980 9358 8980 Size: 378 11293 EcoRV GAT'ATC Cuts at: 4954 4954 Size: 11671 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2210 3535 4520 8659 2210 Size: 1325 985 4139 5222 Fragments arranged by size: 5222 4139 1325 985 FseI GG_CCGG'CC Cuts at: 3989 3989 Size: 11671 FspI TGC'GCA Cuts at: 3395 6599 10177 3395 Size: 3204 3578 4889 Fragments arranged by size: 4889 3578 3204 HaeII r_GCGC'y Cuts at: 1678 3820 5886 6261 6584 7114 7231 7879 8717 Size: 2142 2066 375 323 530 117 648 838 Cuts at: 8717 10818 1678 Size: 2101 2531 Fragments arranged by size: 2531 2142 2101 2066 838 648 530 375 323 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3740 3772 5019 5051 5132 Size: 32 135 32 2168 32 1247 32 81 Cuts at: 5132 5164 5520 5552 5984 5999 6016 6031 6497 Size: 32 356 32 432 15 17 15 466 Cuts at: 6497 6503 6529 6535 7574 7606 10312 10344 10386 Size: 6 26 6 1039 32 2706 32 42 Cuts at: 10386 10418 1373 Size: 32 2626 Fragments arranged by size: 2706 2626 2168 1247 1039 466 432 356 135 81 42 32 32 32 32 32 32 32 32 32 26 17 15 15 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3643 9015 9261 669 Size: 988 281 1705 5372 246 3079 Fragments arranged by size: 5372 3079 1705 988 281 246 HindIII A'AGCT_T Cuts at: 748 3111 3192 7866 748 Size: 2363 81 4674 4553 Fragments arranged by size: 4674 4553 2363 81 HpaI GTT'AAC Cuts at: 9261 9261 Size: 11671 KasI G'GCGC_C Cuts at: 5882 6580 5882 Size: 698 10973 KpnI G_GTAC'C Cuts at: 2814 4234 8996 2814 Size: 1420 4762 5489 Fragments arranged by size: 5489 4762 1420 MfeI C'AATT_G Cuts at: 9270 9270 Size: 11671 MluI A'CGCG_T Cuts at: 11547 11547 Size: 11671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3573 3846 5352 10785 10969 1305 Size: 2268 273 1506 5433 184 2007 Fragments arranged by size: 5433 2268 2007 1506 273 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2132 3090 3223 3977 Size: 54 1425 343 243 57 958 133 754 Cuts at: 3977 4536 5033 5393 7208 7727 8225 8633 8877 Size: 559 497 360 1815 519 498 408 244 Cuts at: 8877 8958 10 Size: 81 2723 Fragments arranged by size: 2723 1815 1425 958 754 559 519 498 497 408 360 343 244 243 133 81 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 2759 2829 2877 3897 4683 4871 Size: 648 1173 420 70 48 1020 786 188 Cuts at: 4871 5447 5670 6593 7371 7404 7475 7832 8687 Size: 576 223 923 778 33 71 357 855 Cuts at: 8687 8867 9629 9988 10147 518 Size: 180 762 359 159 2042 Fragments arranged by size: 2042 1173 1020 923 855 786 778 762 648 576 420 359 357 223 188 180 159 71 70 48 33 MspA1I CmG'CkG Cuts at: 1606 2020 2090 2161 2216 3295 3556 3721 3897 Size: 414 70 71 55 1079 261 165 176 Cuts at: 3897 4204 5170 5317 5513 5768 5788 6071 6289 Size: 307 966 147 196 255 20 283 218 Cuts at: 6289 6619 7036 7162 7282 7339 7570 7624 7759 Size: 330 417 126 120 57 231 54 135 Cuts at: 7759 7840 8329 8627 8867 9087 9764 10912 11157 Size: 81 489 298 240 220 677 1148 245 Cuts at: 11157 1606 Size: 2120 Fragments arranged by size: 2120 1148 1079 966 677 489 417 414 330 307 298 283 261 255 245 240 231 220 218 196 176 165 147 135 126 120 81 71 70 57 55 54 20 NaeI GCC'GGC Cuts at: 2708 2741 3139 3840 3874 3987 4910 5857 6893 Size: 33 398 701 34 113 923 947 1036 Cuts at: 6893 2708 Size: 7486 Fragments arranged by size: 7486 1036 947 923 701 398 113 34 33 NarI GG'CG_CC Cuts at: 5883 6581 5883 Size: 698 10973 NcoI C'CATG_G Cuts at: 513 1065 3228 3436 4828 5634 8165 8604 513 Size: 552 2163 208 1392 806 2531 439 3580 Fragments arranged by size: 3580 2531 2163 1392 806 552 439 208 NdeI CA'TA_TG Cuts at: 387 387 Size: 11671 NgoMIV G'CCGG_C Cuts at: 2706 2739 3137 3838 3872 3985 4908 5855 6891 Size: 33 398 701 34 113 923 947 1036 Cuts at: 6891 2706 Size: 7486 Fragments arranged by size: 7486 1036 947 923 701 398 113 34 33 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11671 NotI GC'GGCC_GC Cuts at: 9118 9118 Size: 11671 NsiI A_TGCA'T Cuts at: 8110 8110 Size: 11671 NspI r_CATG'y Cuts at: 1132 1229 3359 7768 8150 8671 9029 10574 1132 Size: 97 2130 4409 382 521 358 1545 2229 Fragments arranged by size: 4409 2229 2130 1545 521 382 358 97 PasI CC'CwG_GG Cuts at: 1348 2614 6785 8173 1348 Size: 1266 4171 1388 4846 Fragments arranged by size: 4846 4171 1388 1266 PciI A'CATG_T Cuts at: 8667 10570 8667 Size: 1903 9768 PflMI CCAn_nnn'nTGG Cuts at: 1108 2338 2638 4356 6382 7285 7870 8332 1108 Size: 1230 300 1718 2026 903 585 462 4447 Fragments arranged by size: 4447 2026 1718 1230 903 585 462 300 PfoI T'CCnGG_A Cuts at: 3009 3492 3699 6266 8259 8890 3009 Size: 483 207 2567 1993 631 5790 Fragments arranged by size: 5790 2567 1993 631 483 207 PmeI GTTT'AAAC Cuts at: 8975 8975 Size: 11671 PmlI CAC'GTG Cuts at: 4595 4595 Size: 11671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2384 2416 5367 5399 9740 9772 11253 11285 2384 Size: 32 2951 32 4341 32 1481 32 2770 Fragments arranged by size: 4341 2951 2770 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2428 2460 3784 5188 5257 5575 5956 6249 7147 Size: 32 1324 1404 69 318 381 293 898 Cuts at: 7147 7955 8267 8353 8779 2428 Size: 808 312 86 426 5320 Fragments arranged by size: 5320 1404 1324 898 808 426 381 318 312 293 86 69 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11671 PsiI TTA'TAA Cuts at: 9241 9241 Size: 11671 PspOMI G'GGCC_C Cuts at: 2627 4119 5704 6155 6877 7563 7670 8403 8882 Size: 1492 1585 451 722 686 107 733 479 Cuts at: 8882 2627 Size: 5416 Fragments arranged by size: 5416 1585 1492 733 722 686 479 451 107 PspXI vC'TCGA_Gb Cuts at: 3446 6035 3446 Size: 2589 9082 PstI C_TGCA'G Cuts at: 830 2160 2166 2235 2256 2295 3146 3945 4128 Size: 1330 6 69 21 39 851 799 183 Cuts at: 4128 4143 5322 5821 6192 6225 6453 7332 7348 Size: 15 1179 499 371 33 228 879 16 Cuts at: 7348 8922 9023 830 Size: 1574 101 3478 Fragments arranged by size: 3478 1574 1330 1179 879 851 799 499 371 228 183 101 69 39 33 21 16 15 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11671 PvuII CAG'CTG Cuts at: 2161 2216 3295 3556 5170 5317 5513 5768 5788 Size: 55 1079 261 1614 147 196 255 20 Cuts at: 5788 6289 6619 7036 7282 7339 7570 7759 7840 Size: 501 330 417 246 57 231 189 81 Cuts at: 7840 8329 8867 2161 Size: 489 538 4965 Fragments arranged by size: 4965 1614 1079 538 501 489 417 330 261 255 246 231 196 189 147 81 57 55 20 SacI G_AGCT'C Cuts at: 3447 7032 3447 Size: 3585 8086 SacII CC_GC'GG Cuts at: 2021 2091 3898 2021 Size: 70 1807 9794 Fragments arranged by size: 9794 1807 70 SalI G'TCGA_C Cuts at: 1936 9013 1936 Size: 7077 4594 SanDI GG'GwC_CC Cuts at: 2460 7147 7955 8267 2460 Size: 4687 808 312 5864 Fragments arranged by size: 5864 4687 808 312 SapI GCTCTTCn'nnn_ Cuts at: 5834 6276 7253 8738 5834 Size: 442 977 1485 8767 Fragments arranged by size: 8767 1485 977 442 SbfI CC_TGCA'GG Cuts at: 2256 6192 9023 2256 Size: 3936 2831 4904 Fragments arranged by size: 4904 3936 2831 ScaI AGT'ACT Cuts at: 5277 8963 9919 11631 5277 Size: 3686 956 1712 5317 Fragments arranged by size: 5317 3686 1712 956 SexAI A'CCwGG_T Cuts at: 5862 5862 Size: 11671 SfcI C'TryA_G Cuts at: 826 1045 2156 2162 2231 2252 2291 3142 3941 Size: 219 1111 6 69 21 39 851 799 Cuts at: 3941 4124 4139 4511 5118 5318 5599 5817 6188 Size: 183 15 372 607 200 281 218 371 Cuts at: 6188 6221 6449 7328 7344 8515 8918 9019 10154 Size: 33 228 879 16 1171 403 101 1135 Cuts at: 10154 10835 11026 826 Size: 681 191 1471 Fragments arranged by size: 1471 1171 1135 1111 879 851 799 681 607 403 372 371 281 228 219 218 200 191 183 101 69 39 33 21 16 15 6 SfiI GGCCn_nnn'nGGCC Cuts at: 7724 7724 Size: 11671 SfoI GGC'GCC Cuts at: 5884 6582 5884 Size: 698 10973 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11671 SmaI CCC'GGG Cuts at: 1872 3462 5096 7055 7562 8317 8998 1872 Size: 1590 1634 1959 507 755 681 4545 Fragments arranged by size: 4545 1959 1634 1590 755 681 507 SmlI C'TyrA_G Cuts at: 820 1017 1943 3344 3446 5418 6035 6240 8357 Size: 197 926 1401 102 1972 617 205 2117 Cuts at: 8357 8657 8948 9775 10676 10938 820 Size: 300 291 827 901 262 1553 Fragments arranged by size: 2117 1972 1553 1401 926 901 827 617 300 291 262 205 197 102 SnaBI TAC'GTA Cuts at: 493 9462 493 Size: 8969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11671 SphI G_CATG'C Cuts at: 1132 9029 1132 Size: 7897 3774 SspI AAT'ATT Cuts at: 5 52 6970 9595 5 Size: 47 6918 2625 2081 Fragments arranged by size: 6918 2625 2081 47 StuI AGG'CCT Cuts at: 1517 2996 3743 5369 5984 6021 6447 7463 7514 Size: 1479 747 1626 615 37 426 1016 51 Cuts at: 7514 1517 Size: 5674 Fragments arranged by size: 5674 1626 1479 1016 747 615 426 51 37 StyI C'CwwG_G Cuts at: 513 1065 2049 2464 2718 3228 3436 3787 4027 Size: 552 984 415 254 510 208 351 240 Cuts at: 4027 4828 5634 5886 8165 8604 8634 9110 513 Size: 801 806 252 2279 439 30 476 3074 Fragments arranged by size: 3074 2279 984 806 801 552 510 476 439 415 351 254 252 240 208 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 3305 3860 4409 4562 5264 5917 Size: 1255 579 1146 555 549 153 702 653 Cuts at: 5917 6014 7397 7646 8950 8956 9698 9715 9868 Size: 97 1383 249 1304 6 742 17 153 Cuts at: 9868 10053 10498 325 Size: 185 445 1498 Fragments arranged by size: 1498 1383 1304 1255 1146 742 702 653 579 555 549 445 249 185 153 153 97 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5275 8961 9917 Size: 274 80 33 51 1443 3298 3686 956 Cuts at: 9917 11629 96 Size: 1712 138 Fragments arranged by size: 3686 3298 1712 1443 956 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4179 4211 8439 8471 11631 11663 4179 Size: 32 4228 32 3160 32 4187 Fragments arranged by size: 4228 4187 3160 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11671 XbaI T'CTAG_A Cuts at: 9007 9007 Size: 11671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 3967 4063 4420 5353 5510 7075 7174 8188 Size: 1362 96 357 933 157 1565 99 1014 Cuts at: 8188 2605 Size: 6088 Fragments arranged by size: 6088 1565 1362 1014 933 357 157 99 96 XhoI C'TCGA_G Cuts at: 1943 3446 6035 1943 Size: 1503 2589 7579 Fragments arranged by size: 7579 2589 1503 XmaI C'CCGG_G Cuts at: 1870 3460 5094 7053 7560 8315 8996 1870 Size: 1590 1634 1959 507 755 681 4545 Fragments arranged by size: 4545 1959 1634 1590 755 681 507 XmnI GAAnn'nnTTC Cuts at: 9800 9800 Size: 11671 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8773 9479 276 Size: 53 83 186 936 7239 706 2468 Fragments arranged by size: 7239 2468 936 706 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I FspAI NruI PacI PsrI RsrII SrfI SwaI