(Linear) (Six-base) MAPSORT of: KIBB9563.seq Check: 2768 from: 1 to: 6966 KIBB9563.orf With 182 enzymes: SgfI * March 3, 2008 11:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 785 2140 2280 3306 3819 4176 6142 6403 Size: 785 1355 140 1026 513 357 1966 261 Cuts at: 6403 6966 Size: 563 Fragments arranged by size: 1966 1355 1026 785 563 513 357 261 140 AatII G_ACGT'C Cuts at: 0 6770 6966 Size: 6770 196 AccI GT'mk_AC Cuts at: 0 2912 4529 4952 6966 Size: 2912 1617 423 2014 Fragments arranged by size: 2912 2014 1617 423 Acc65I G'GTAC_C Cuts at: 0 805 2225 6966 Size: 805 1420 4741 Fragments arranged by size: 4741 1420 805 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 599 1981 3114 3736 4077 4771 5982 6054 Size: 599 1382 1133 622 341 694 1211 72 Cuts at: 6054 6966 Size: 912 Fragments arranged by size: 1382 1211 1133 912 694 622 599 341 72 AfeI AGC'GCT Cuts at: 0 5224 6966 Size: 5224 1742 AflII C'TTAA_G Cuts at: 0 6943 6966 Size: 6943 23 AflIII A'CryG_T Cuts at: 0 6662 6966 Size: 6662 304 AhdI GACnn_n'nnGTC Cuts at: 0 3529 4512 6966 Size: 3529 983 2454 Fragments arranged by size: 3529 2454 983 AleI CACnn'nnGTG Cuts at: 0 334 824 1892 2678 2866 3442 4588 5366 Size: 334 490 1068 786 188 576 1146 778 Cuts at: 5366 5399 6862 6966 Size: 33 1463 104 Fragments arranged by size: 1463 1146 1068 786 778 576 490 334 188 104 33 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1152 2026 3683 4606 6966 Size: 1152 874 1657 923 2360 Fragments arranged by size: 2360 1657 1152 923 874 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1900 1932 3362 3394 6966 Size: 1900 32 1430 32 3572 Fragments arranged by size: 3572 1900 1430 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1293 1404 3292 3573 4191 4767 4837 4851 Size: 1293 111 1888 281 618 576 70 14 Cuts at: 4851 5593 6777 6966 Size: 742 1184 189 Fragments arranged by size: 1888 1293 1184 742 618 576 281 189 111 70 14 ApaI G_GGCC'C Cuts at: 0 626 2118 3703 4154 4876 5562 5669 6402 Size: 626 1492 1585 451 722 686 107 733 Cuts at: 6402 6881 6966 Size: 479 85 Fragments arranged by size: 1585 1492 733 722 686 626 479 451 107 85 ApaLI G'TGCA_C Cuts at: 0 3280 3658 6966 Size: 3280 378 3308 Fragments arranged by size: 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265 261 248 218 189 176 138 121 107 91 85 61 30 23 22 BbeI G_GCGC'C Cuts at: 0 3881 4579 6966 Size: 3881 698 2387 Fragments arranged by size: 3881 2387 698 BbsI GAAGACnn'nnnn_ Cuts at: 0 1073 2894 3009 3738 6966 Size: 1073 1821 115 729 3228 Fragments arranged by size: 3228 1821 1073 729 115 BbvCI CC'TCA_GC Cuts at: 0 280 396 937 3613 4177 4249 5498 5641 Size: 280 116 541 2676 564 72 1249 143 Cuts at: 5641 6966 Size: 1325 Fragments arranged by size: 2676 1325 1249 564 541 280 143 116 72 BfrBI ATG'CAT Cuts at: 0 6103 6966 Size: 6103 863 BglI GCCn_nnn'nGGC Cuts at: 0 724 1210 1719 2185 2599 3225 3605 3799 Size: 724 486 509 466 414 626 380 194 Cuts at: 3799 5434 5719 6966 Size: 1635 285 1247 Fragments arranged by size: 1635 1247 724 626 509 486 466 414 380 285 194 BglII A'GATC_T Cuts at: 0 3737 6966 Size: 3737 3229 BlpI GC'TnA_GC Cuts at: 0 1382 5755 6966 Size: 1382 4373 1211 Fragments arranged by size: 4373 1382 1211 Bme1580I G_kGCm'C Cuts at: 0 451 626 658 1330 1428 1586 2118 2515 Size: 451 175 32 672 98 158 532 397 Cuts at: 2515 3284 3430 3662 3703 4154 4585 4876 5377 Size: 769 146 232 41 451 431 291 501 Cuts at: 5377 5406 5547 5562 5625 5669 5844 5858 6293 Size: 29 141 15 63 44 175 14 435 Cuts at: 6293 6402 6444 6881 6966 Size: 109 42 437 85 Fragments arranged by size: 769 672 532 501 451 451 437 435 431 397 291 232 175 175 158 146 141 109 98 85 63 44 42 41 32 29 15 14 BmgBI CAC'GTC Cuts at: 0 170 1494 3123 3501 5055 6966 Size: 170 1324 1629 378 1554 1911 Fragments arranged by size: 1911 1629 1554 1324 378 170 BmrI ACTGGGnnnn_n' Cuts at: 0 461 2120 2320 5949 6966 Size: 461 1659 200 3629 1017 Fragments arranged by size: 3629 1659 1017 461 200 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 952 1557 6966 Size: 952 605 5409 Fragments arranged by size: 5409 952 605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 278 967 988 1165 1579 2302 2386 Size: 133 145 689 21 177 414 723 84 Cuts at: 2386 2440 2452 3373 3381 3805 4002 4072 4213 Size: 54 12 921 8 424 197 70 141 Cuts at: 4213 4284 4809 5011 5395 5869 6277 6394 6451 Size: 71 525 202 384 474 408 117 57 Cuts at: 6451 6869 6966 Size: 418 97 Fragments arranged by size: 921 723 689 525 474 424 418 414 408 384 202 197 177 145 141 133 117 97 84 71 70 57 54 21 12 8 Bpu10I CC'TnA_GC Cuts at: 0 280 396 937 1235 1250 2230 2308 3613 Size: 280 116 541 298 15 980 78 1305 Cuts at: 3613 4177 4249 5498 5641 6614 6643 6966 Size: 564 72 1249 143 973 29 323 Fragments arranged by size: 1305 1249 980 973 564 541 323 298 280 143 116 78 72 29 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1360 3398 4220 6373 6673 6966 Size: 1360 2038 822 2153 300 293 Fragments arranged by size: 2153 2038 1360 822 300 293 BsaI GGTCTCn'nnnn_ Cuts at: 0 1811 2059 2392 2926 5140 6811 6966 Size: 1811 248 333 534 2214 1671 155 Fragments arranged by size: 2214 1811 1671 534 333 248 155 BsaAI yAC'GTr Cuts at: 0 2590 4296 6966 Size: 2590 1706 2670 Fragments arranged by size: 2670 2590 1706 BsaHI Gr'CG_yC Cuts at: 0 243 3158 3878 4037 4576 6449 6767 6966 Size: 243 2915 720 159 539 1873 318 199 Fragments arranged by size: 2915 1873 720 539 318 243 199 159 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1141 1171 3362 3392 3949 3979 5590 5620 Size: 1141 30 2191 30 557 30 1611 30 Cuts at: 5620 6037 6067 6686 6716 6966 Size: 417 30 619 30 250 Fragments arranged by size: 2191 1611 1141 619 557 417 250 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 289 384 529 944 1162 1165 1168 1782 Size: 289 95 145 415 218 3 3 614 Cuts at: 1782 2449 2707 3016 3178 4506 4732 5305 5865 Size: 667 258 309 162 1328 226 573 560 Cuts at: 5865 5932 6042 6045 6966 Size: 67 110 3 921 Fragments arranged by size: 1328 921 667 614 573 560 415 309 289 258 226 218 162 145 110 95 67 3 3 3 BseYI C'CCAG_C Cuts at: 0 173 207 356 1053 1286 1716 2390 3161 Size: 173 34 149 697 233 430 674 771 Cuts at: 3161 3518 3563 3898 4202 4546 4610 4844 4877 Size: 357 45 335 304 344 64 234 33 Cuts at: 4877 4901 4957 5102 5110 5219 5263 5305 5330 Size: 24 56 145 8 109 44 42 25 Cuts at: 5330 5378 5561 6366 6966 Size: 48 183 805 600 Fragments arranged by size: 805 771 697 674 600 430 357 344 335 304 234 233 183 173 149 145 109 64 56 48 45 44 42 34 33 25 24 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1375 1886 2308 2359 3079 3379 3934 3946 Size: 1375 511 422 51 720 300 555 12 Cuts at: 3946 5851 6966 Size: 1905 1115 Fragments arranged by size: 1905 1375 1115 720 555 511 422 300 51 12 BsiEI CG_ry'CG Cuts at: 0 1841 1981 3443 6966 Size: 1841 140 1462 3523 Fragments arranged by size: 3523 1841 1462 140 BsiHKAI G_wGCw'C Cuts at: 0 1442 2236 3284 3449 3662 4026 4498 4694 Size: 1442 794 1048 165 213 364 472 196 Cuts at: 4694 4742 5027 5243 6966 Size: 48 285 216 1723 Fragments arranged by size: 1723 1442 1048 794 472 364 285 216 213 196 165 48 BsmBI CGTCTCn'nnnn_ Cuts at: 0 295 1083 6966 Size: 295 788 5883 Fragments arranged by size: 5883 788 295 Bsp1286I G_dGCh'C Cuts at: 0 414 437 451 626 658 1330 1428 1442 Size: 414 23 14 175 32 672 98 14 Cuts at: 1442 1464 1586 2118 2236 2515 3221 3284 3430 Size: 22 122 532 118 279 706 63 146 Cuts at: 3430 3449 3662 3703 3841 4026 4154 4215 4498 Size: 19 213 41 138 185 128 61 283 Cuts at: 4498 4585 4628 4694 4742 4876 4997 5027 5243 Size: 87 43 66 48 134 121 30 216 Cuts at: 5243 5297 5377 5406 5547 5562 5625 5669 5844 Size: 54 80 29 141 15 63 44 175 Cuts at: 5844 5858 6135 6293 6311 6402 6444 6620 6881 Size: 14 277 158 18 91 42 176 261 Cuts at: 6881 6966 Size: 85 Fragments arranged by size: 706 672 532 414 283 279 277 261 216 213 185 176 175 175 158 146 141 138 134 128 122 121 118 98 91 87 85 80 66 63 63 61 54 48 44 43 42 41 32 30 29 23 22 19 18 15 14 14 14 BspHI T'CATG_A Cuts at: 0 6060 6966 Size: 6060 906 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 158 240 785 2140 2280 3306 3581 3819 Size: 158 82 545 1355 140 1026 275 238 Cuts at: 3819 3968 4176 6142 6403 6966 Size: 149 208 1966 261 563 Fragments arranged by size: 1966 1355 1026 563 545 275 261 238 208 158 149 140 82 BsrBI CCG'CTC Cuts at: 0 2814 5490 6855 6966 Size: 2814 2676 1365 111 Fragments arranged by size: 2814 2676 1365 111 BsrDI GCAATG_nn' Cuts at: 0 3686 6966 Size: 3686 3280 BsrFI r'CCGG_y Cuts at: 0 701 734 881 1132 1833 1867 1980 2903 Size: 701 33 147 251 701 34 113 923 Cuts at: 2903 3850 4886 6966 Size: 947 1036 2080 Fragments arranged by size: 2080 1036 947 923 701 701 251 147 113 34 33 BssSI C'ACGA_G Cuts at: 0 2719 4627 5540 6966 Size: 2719 1908 913 1426 Fragments arranged by size: 2719 1908 1426 913 BstAPI GCAn_nnn'nTGC Cuts at: 0 3573 4983 5238 6966 Size: 3573 1410 255 1728 Fragments arranged by size: 3573 1728 1410 255 BstBI TT'CG_AA Cuts at: 0 922 6966 Size: 922 6044 BstEII G'GTnAC_C Cuts at: 0 2568 2683 6966 Size: 2568 115 4283 Fragments arranged by size: 4283 2568 115 BstXI CCAn_nnnn'nTGG Cuts at: 0 1783 1894 2076 2680 3405 4170 5199 5368 Size: 1783 111 182 604 725 765 1029 169 Cuts at: 5368 5386 5796 6690 6966 Size: 18 410 894 276 Fragments arranged by size: 1783 1029 894 765 725 604 410 276 182 169 111 18 BstYI r'GATC_y Cuts at: 0 1991 2303 3074 3305 3737 4521 6966 Size: 1991 312 771 231 432 784 2445 Fragments arranged by size: 2445 1991 784 771 432 312 231 Bsu36I CC'TnA_GG Cuts at: 0 475 496 1208 1724 2729 3256 3974 4011 Size: 475 21 712 516 1005 527 718 37 Cuts at: 4011 4103 4813 6966 Size: 92 710 2153 Fragments arranged by size: 2153 1005 718 712 710 527 516 475 92 37 21 BtgI C'CryG_G Cuts at: 0 13 83 128 785 896 952 1223 1431 Size: 13 70 45 657 111 56 271 208 Cuts at: 1431 1805 1890 2075 2264 2823 3297 3629 3641 Size: 374 85 185 189 559 474 332 12 Cuts at: 3641 5171 5361 5432 6160 6599 6966 Size: 1530 190 71 728 439 367 Fragments arranged by size: 1530 728 657 559 474 439 374 367 332 271 208 190 189 185 111 85 71 70 56 45 13 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1943 3149 4001 6966 Size: 1943 1206 852 2965 Fragments arranged by size: 2965 1943 1206 852 BtsI GCAGTG_nn' Cuts at: 0 2371 2988 3341 3483 3809 3941 4132 4463 Size: 2371 617 353 142 326 132 191 331 Cuts at: 4463 6240 6966 Size: 1777 726 Fragments arranged by size: 2371 1777 726 617 353 331 326 191 142 132 DraIII CAC_nnn'GTG Cuts at: 0 333 2351 3847 6966 Size: 333 2018 1496 3119 Fragments arranged by size: 3119 2018 1496 333 DrdI GACnn_nn'nnGTC Cuts at: 0 1075 4330 6196 6772 6966 Size: 1075 3255 1866 576 194 Fragments arranged by size: 3255 1866 1075 576 194 EaeI y'GGCC_r Cuts at: 0 68 125 528 836 1083 1216 1316 1952 Size: 68 57 403 308 247 133 100 636 Cuts at: 1952 1970 1978 1982 2529 2562 2901 3026 3206 Size: 18 8 4 547 33 339 125 180 Cuts at: 3206 3386 3440 5057 5201 5720 6218 6273 6626 Size: 180 54 1617 144 519 498 55 353 Cuts at: 6626 6870 6951 6966 Size: 244 81 15 Fragments arranged by size: 1617 636 547 519 498 403 353 339 308 247 244 180 180 144 133 125 100 81 68 57 55 54 33 18 15 8 4 EagI C'GGCC_G Cuts at: 0 1978 3440 6966 Size: 1978 1462 3526 Fragments arranged by size: 3526 1978 1462 EarI CTCTTCn'nnn_ Cuts at: 0 575 3256 3829 4271 5248 6733 6966 Size: 575 2681 573 442 977 1485 233 Fragments arranged by size: 2681 1485 977 575 573 442 233 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4663 5808 6238 6966 Size: 4663 1145 430 728 Fragments arranged by size: 4663 1145 728 430 EcoICRI GAG'CTC Cuts at: 0 1440 5025 6966 Size: 1440 3585 1941 Fragments arranged by size: 3585 1941 1440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 278 599 967 988 1165 1579 1981 Size: 133 145 321 368 21 177 414 402 Cuts at: 1981 2302 2386 2440 2452 3114 3373 3381 3736 Size: 321 84 54 12 662 259 8 355 Cuts at: 3736 3805 4002 4072 4077 4213 4284 4771 4809 Size: 69 197 70 5 136 71 487 38 Cuts at: 4809 5011 5395 5869 5982 6054 6277 6394 6451 Size: 202 384 474 113 72 223 117 57 Cuts at: 6451 6869 6966 Size: 418 97 Fragments arranged by size: 662 487 474 418 414 402 384 368 355 321 321 259 223 202 197 177 145 136 133 117 113 97 84 72 71 70 69 57 54 38 21 12 8 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 474 1253 1375 2434 2800 3416 4219 5944 Size: 474 779 122 1059 366 616 803 1725 Cuts at: 5944 6256 6887 6966 Size: 312 631 79 Fragments arranged by size: 1725 1059 803 779 631 616 474 366 312 122 79 EcoO109I rG'GnC_Cy Cuts at: 0 423 455 623 1720 1764 1779 2114 2115 Size: 423 32 168 1097 44 15 335 1 Cuts at: 2115 3183 3252 3570 3699 3951 4151 4244 4872 Size: 1068 69 318 129 252 200 93 628 Cuts at: 4872 4873 4923 5142 5426 5665 5950 6262 6348 Size: 1 50 219 284 239 285 312 86 Cuts at: 6348 6398 6399 6595 6774 6877 6878 6966 Size: 50 1 196 179 103 1 88 Fragments arranged by size: 1097 1068 628 423 335 318 312 285 284 252 239 219 200 196 179 168 129 103 93 88 86 69 50 50 44 32 15 1 1 1 1 EcoRV GAT'ATC Cuts at: 0 2949 6966 Size: 2949 4017 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 205 1530 2515 6654 6966 Size: 205 1325 985 4139 312 Fragments arranged by size: 4139 1325 985 312 205 FseI GG_CCGG'CC Cuts at: 0 1984 6966 Size: 1984 4982 FspI TGC'GCA Cuts at: 0 1390 4594 6966 Size: 1390 3204 2372 Fragments arranged by size: 3204 2372 1390 HaeII r_GCGC'y Cuts at: 0 1815 3881 4256 4579 5109 5226 5874 6712 Size: 1815 2066 375 323 530 117 648 838 Cuts at: 6712 6966 Size: 254 Fragments arranged by size: 2066 1815 838 648 530 375 323 254 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1735 1767 3014 3046 3127 3159 3515 3547 Size: 1735 32 1247 32 81 32 356 32 Cuts at: 3547 3979 3994 4011 4026 4492 4498 4524 4530 Size: 432 15 17 15 466 6 26 6 Cuts at: 4530 5569 5601 6966 Size: 1039 32 1365 Fragments arranged by size: 1735 1365 1247 1039 466 432 356 81 32 32 32 32 32 26 17 15 15 6 6 HincII GTy'rAC Cuts at: 0 1638 6966 Size: 1638 5328 HindIII A'AGCT_T Cuts at: 0 1106 1187 5861 6966 Size: 1106 81 4674 1105 Fragments arranged by size: 4674 1106 1105 81 KasI G'GCGC_C Cuts at: 0 3877 4575 6966 Size: 3877 698 2391 Fragments arranged by size: 3877 2391 698 KpnI G_GTAC'C Cuts at: 0 809 2229 6966 Size: 809 1420 4737 Fragments arranged by size: 4737 1420 809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1568 1841 3347 6966 Size: 1568 273 1506 3619 Fragments arranged by size: 3619 1568 1506 273 MscI TGG'CCA Cuts at: 0 70 127 1085 1218 1972 2531 3028 3388 Size: 70 57 958 133 754 559 497 360 Cuts at: 3388 5203 5722 6220 6628 6872 6953 6966 Size: 1815 519 498 408 244 81 13 Fragments arranged by size: 1815 958 754 559 519 498 497 408 360 244 133 81 70 57 13 MslI CAynn'nnrTG Cuts at: 0 334 754 824 872 1892 2678 2866 3442 Size: 334 420 70 48 1020 786 188 576 Cuts at: 3442 3665 4588 5366 5399 5470 5827 6682 6862 Size: 223 923 778 33 71 357 855 180 Cuts at: 6862 6966 Size: 104 Fragments arranged by size: 1020 923 855 786 778 576 420 357 334 223 188 180 104 71 70 48 33 MspA1I CmG'CkG Cuts at: 0 15 85 156 211 1290 1551 1716 1892 Size: 15 70 71 55 1079 261 165 176 Cuts at: 1892 2199 3165 3312 3508 3763 3783 4066 4284 Size: 307 966 147 196 255 20 283 218 Cuts at: 4284 4614 5031 5157 5277 5334 5565 5619 5754 Size: 330 417 126 120 57 231 54 135 Cuts at: 5754 5835 6324 6622 6862 6966 Size: 81 489 298 240 104 Fragments arranged by size: 1079 966 489 417 330 307 298 283 261 255 240 231 218 196 176 165 147 135 126 120 104 81 71 70 57 55 54 20 15 NaeI GCC'GGC Cuts at: 0 703 736 1134 1835 1869 1982 2905 3852 Size: 703 33 398 701 34 113 923 947 Cuts at: 3852 4888 6966 Size: 1036 2078 Fragments arranged by size: 2078 1036 947 923 703 701 398 113 34 33 NarI GG'CG_CC Cuts at: 0 3878 4576 6966 Size: 3878 698 2390 Fragments arranged by size: 3878 2390 698 NcoI C'CATG_G Cuts at: 0 1223 1431 2823 3629 6160 6599 6966 Size: 1223 208 1392 806 2531 439 367 Fragments arranged by size: 2531 1392 1223 806 439 367 208 NgoMIV G'CCGG_C Cuts at: 0 701 734 1132 1833 1867 1980 2903 3850 Size: 701 33 398 701 34 113 923 947 Cuts at: 3850 4886 6966 Size: 1036 2080 Fragments arranged by size: 2080 1036 947 923 701 701 398 113 34 33 NsiI A_TGCA'T Cuts at: 0 6105 6966 Size: 6105 861 NspI r_CATG'y Cuts at: 0 1354 5763 6145 6666 6966 Size: 1354 4409 382 521 300 Fragments arranged by size: 4409 1354 521 382 300 PasI CC'CwG_GG Cuts at: 0 609 4780 6168 6966 Size: 609 4171 1388 798 Fragments arranged by size: 4171 1388 798 609 PciI A'CATG_T Cuts at: 0 6662 6966 Size: 6662 304 PflMI CCAn_nnn'nTGG Cuts at: 0 333 633 2351 4377 5280 5865 6327 6966 Size: 333 300 1718 2026 903 585 462 639 Fragments arranged by size: 2026 1718 903 639 585 462 333 300 PfoI T'CCnGG_A Cuts at: 0 1004 1487 1694 4261 6254 6885 6966 Size: 1004 483 207 2567 1993 631 81 Fragments arranged by size: 2567 1993 1004 631 483 207 81 PmlI CAC'GTG Cuts at: 0 2590 6966 Size: 2590 4376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 379 411 3362 3394 6966 Size: 379 32 2951 32 3572 Fragments arranged by size: 3572 2951 379 32 32 PpuMI rG'GwC_Cy Cuts at: 0 423 455 1779 3183 3252 3570 3951 4244 Size: 423 32 1324 1404 69 318 381 293 Cuts at: 4244 5142 5950 6262 6348 6774 6966 Size: 898 808 312 86 426 192 Fragments arranged by size: 1404 1324 898 808 426 423 381 318 312 293 192 86 69 32 PspOMI G'GGCC_C Cuts at: 0 622 2114 3699 4150 4872 5558 5665 6398 Size: 622 1492 1585 451 722 686 107 733 Cuts at: 6398 6877 6966 Size: 479 89 Fragments arranged by size: 1585 1492 733 722 686 622 479 451 107 89 PspXI vC'TCGA_Gb Cuts at: 0 1441 4030 6966 Size: 1441 2589 2936 Fragments arranged by size: 2936 2589 1441 PstI C_TGCA'G Cuts at: 0 155 161 230 251 290 1141 1940 2123 Size: 155 6 69 21 39 851 799 183 Cuts at: 2123 2138 3317 3816 4187 4220 4448 5327 5343 Size: 15 1179 499 371 33 228 879 16 Cuts at: 5343 6917 6966 Size: 1574 49 Fragments arranged by size: 1574 1179 879 851 799 499 371 228 183 155 69 49 39 33 21 16 15 6 PvuII CAG'CTG Cuts at: 0 156 211 1290 1551 3165 3312 3508 3763 Size: 156 55 1079 261 1614 147 196 255 Cuts at: 3763 3783 4284 4614 5031 5277 5334 5565 5754 Size: 20 501 330 417 246 57 231 189 Cuts at: 5754 5835 6324 6862 6966 Size: 81 489 538 104 Fragments arranged by size: 1614 1079 538 501 489 417 330 261 255 246 231 196 189 156 147 104 81 57 55 20 SacI G_AGCT'C Cuts at: 0 1442 5027 6966 Size: 1442 3585 1939 Fragments arranged by size: 3585 1939 1442 SacII CC_GC'GG Cuts at: 0 16 86 1893 6966 Size: 16 70 1807 5073 Fragments arranged by size: 5073 1807 70 16 SanDI GG'GwC_CC Cuts at: 0 455 5142 5950 6262 6966 Size: 455 4687 808 312 704 Fragments arranged by size: 4687 808 704 455 312 SapI GCTCTTCn'nnn_ Cuts at: 0 3829 4271 5248 6733 6966 Size: 3829 442 977 1485 233 Fragments arranged by size: 3829 1485 977 442 233 SbfI CC_TGCA'GG Cuts at: 0 251 4187 6966 Size: 251 3936 2779 Fragments arranged by size: 3936 2779 251 ScaI AGT'ACT Cuts at: 0 3272 6958 6966 Size: 3272 3686 8 Fragments arranged by size: 3686 3272 8 SexAI A'CCwGG_T Cuts at: 0 3857 6966 Size: 3857 3109 SfcI C'TryA_G Cuts at: 0 151 157 226 247 286 1137 1936 2119 Size: 151 6 69 21 39 851 799 183 Cuts at: 2119 2134 2506 3113 3313 3594 3812 4183 4216 Size: 15 372 607 200 281 218 371 33 Cuts at: 4216 4444 5323 5339 6510 6913 6966 Size: 228 879 16 1171 403 53 Fragments arranged by size: 1171 879 851 799 607 403 372 371 281 228 218 200 183 151 69 53 39 33 21 16 15 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 5719 6966 Size: 5719 1247 SfoI GGC'GCC Cuts at: 0 3879 4577 6966 Size: 3879 698 2389 Fragments arranged by size: 3879 2389 698 SmaI CCC'GGG Cuts at: 0 1457 3091 5050 5557 6312 6966 Size: 1457 1634 1959 507 755 654 Fragments arranged by size: 1959 1634 1457 755 654 507 SmlI C'TyrA_G Cuts at: 0 1339 1441 3413 4030 4235 6352 6652 6943 Size: 1339 102 1972 617 205 2117 300 291 Cuts at: 6943 6966 Size: 23 Fragments arranged by size: 2117 1972 1339 617 300 291 205 102 23 SspI AAT'ATT Cuts at: 0 4965 6966 Size: 4965 2001 StuI AGG'CCT Cuts at: 0 991 1738 3364 3979 4016 4442 5458 5509 Size: 991 747 1626 615 37 426 1016 51 Cuts at: 5509 6966 Size: 1457 Fragments arranged by size: 1626 1457 1016 991 747 615 426 51 37 StyI C'CwwG_G Cuts at: 0 44 459 713 1223 1431 1782 2022 2823 Size: 44 415 254 510 208 351 240 801 Cuts at: 2823 3629 3881 6160 6599 6629 6966 Size: 806 252 2279 439 30 337 Fragments arranged by size: 2279 806 801 510 439 415 351 337 254 252 240 208 44 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 154 1300 1855 2404 2557 3259 3912 4009 Size: 154 1146 555 549 153 702 653 97 Cuts at: 4009 5392 5641 6945 6951 6966 Size: 1383 249 1304 6 15 Fragments arranged by size: 1383 1304 1146 702 653 555 549 249 154 153 97 15 6 TatI w'GTAC_w Cuts at: 0 3270 6956 6966 Size: 3270 3686 10 Fragments arranged by size: 3686 3270 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2174 2206 6434 6466 6966 Size: 2174 32 4228 32 500 Fragments arranged by size: 4228 2174 500 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 600 1962 2058 2415 3348 3505 5070 5169 Size: 600 1362 96 357 933 157 1565 99 Cuts at: 5169 6183 6966 Size: 1014 783 Fragments arranged by size: 1565 1362 1014 933 783 600 357 157 99 96 XhoI C'TCGA_G Cuts at: 0 1441 4030 6966 Size: 1441 2589 2936 Fragments arranged by size: 2936 2589 1441 XmaI C'CCGG_G Cuts at: 0 1455 3089 5048 5555 6310 6966 Size: 1455 1634 1959 507 755 656 Fragments arranged by size: 1959 1634 1455 755 656 507 ZraI GAC'GTC Cuts at: 0 6768 6966 Size: 6768 198 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: AclI AgeI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BmtI BsaBI BsaWI BsiWI BsmI BspEI BsrGI BssHII BstZ17I ClaI CspCI DraI EcoRI FspAI HpaI MfeI MluI NdeI NheI NotI NruI PacI PmeI PshAI PsiI PsrI PvuI RsrII SalI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XmnI