(Circular) (Six-base) MAPSORT of: pFN21AB9521.seq Check: 4160 from: 1 to: 6469 pFN21AB9521 6469 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2324 2324 Size: 6469 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3812 1937 Size: 1875 4594 Acc65I G'GTAC_C Cuts at: 2705 2725 3790 2705 Size: 20 1065 5384 Fragments arranged by size: 5384 1065 20 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2536 3438 4532 5916 6184 1632 Size: 904 902 1094 1384 268 1917 Fragments arranged by size: 1917 1384 1094 904 902 268 AfeI AGC'GCT Cuts at: 2489 2990 2489 Size: 501 5968 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6272 AflIII A'CryG_T Cuts at: 1203 5368 6345 1203 Size: 4165 977 1327 Fragments arranged by size: 4165 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 5198 5198 Size: 6469 AleI CACnn'nnGTG Cuts at: 1166 2858 1166 Size: 1692 4777 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6469 AlwNI CAG_nnn'CTG Cuts at: 2407 2620 2956 4212 5784 2407 Size: 213 336 1256 1572 3092 Fragments arranged by size: 3092 1572 1256 336 213 ApaI G_GGCC'C Cuts at: 2629 3349 3737 2629 Size: 720 388 5361 Fragments arranged by size: 5361 720 388 ApaLI G'TGCA_C Cuts at: 4526 5682 4526 Size: 1156 5313 ApoI r'AATT_y Cuts at: 1493 3778 4004 4156 6265 1493 Size: 2285 226 152 2109 1697 Fragments arranged by size: 2285 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2751 3273 3350 3794 1870 Size: 73 67 741 522 77 444 4545 Fragments arranged by size: 4545 741 522 444 77 73 67 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2251 2284 2251 Size: 33 6436 BamHI G'GATC_C Cuts at: 3725 3799 4166 3725 Size: 74 367 6028 Fragments arranged by size: 6028 367 74 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2705 2725 2860 2905 3790 Size: 325 217 627 918 20 135 45 885 Cuts at: 3790 5245 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 918 885 627 325 217 135 45 20 BanII G_rGCy'C Cuts at: 1391 2079 2629 2644 3349 3737 3788 1391 Size: 688 550 15 705 388 51 4072 Fragments arranged by size: 4072 705 688 550 388 51 15 BbeI G_GCGC'C Cuts at: 2909 2909 Size: 6469 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2272 928 Size: 971 373 5125 Fragments arranged by size: 5125 971 373 BbvCI CC'TCA_GC Cuts at: 2111 2384 2111 Size: 273 6196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2213 2247 2263 2297 3560 3594 4660 Size: 34 276 34 16 34 1263 34 1066 Cuts at: 4660 4694 1903 Size: 34 3678 Fragments arranged by size: 3678 1263 1066 276 34 34 34 34 34 16 BciVI GTATCCnnnnn_n' Cuts at: 4361 5571 4361 Size: 1210 5259 BclI T'GATC_A Cuts at: 1548 1548 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3175 5080 136 Size: 107 122 71 631 605 1503 1905 1525 Fragments arranged by size: 1905 1525 1503 631 605 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 3886 3886 Size: 6469 Bme1580I G_kGCm'C Cuts at: 1790 2177 2202 2629 2865 3349 3573 3737 4530 Size: 387 25 427 236 484 224 164 793 Cuts at: 4530 5686 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 793 484 427 387 236 224 164 25 BmgBI CAC'GTC Cuts at: 1318 3004 1318 Size: 1686 4783 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3605 4200 5158 Size: 416 53 83 399 24 2188 595 958 Cuts at: 5158 442 Size: 1753 Fragments arranged by size: 2188 1753 958 595 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2053 2053 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 5129 1239 Size: 929 2961 2579 Fragments arranged by size: 2961 2579 929 Bpu10I CC'TnA_GC Cuts at: 2111 2384 2111 Size: 273 6196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2876 3218 4594 5459 5757 2876 Size: 342 1376 865 298 3588 Fragments arranged by size: 3588 1376 865 342 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5132 882 Size: 617 3633 2219 Fragments arranged by size: 3633 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4260 493 Size: 711 3056 2702 Fragments arranged by size: 3056 2702 711 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2906 3235 4276 4658 Size: 53 83 186 936 1373 329 1041 382 Cuts at: 4658 5293 275 Size: 635 1451 Fragments arranged by size: 1451 1373 1041 936 635 382 329 186 83 53 BsaWI w'CCGG_w Cuts at: 4902 5300 5574 5721 6132 4902 Size: 398 274 147 411 5239 Fragments arranged by size: 5239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6244 Fragments arranged by size: 6244 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 3059 3183 3413 3527 3567 1186 Size: 859 1014 124 230 114 40 4088 Fragments arranged by size: 4088 1014 859 230 124 114 40 BseYI C'CCAG_C Cuts at: 3331 3756 5672 6168 3331 Size: 425 1916 496 3632 Fragments arranged by size: 3632 1916 496 425 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 3278 1163 Size: 573 507 1035 4354 Fragments arranged by size: 4354 1035 573 507 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2328 3919 4680 5708 1540 Size: 275 186 327 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 327 275 186 BsiHKAI G_wGCw'C Cuts at: 2942 4530 4615 5686 2942 Size: 1588 85 1071 3725 Fragments arranged by size: 3725 1588 1071 85 BsmI GAATG_Cn' Cuts at: 3980 4073 3980 Size: 93 6376 BsmBI CGTCTCn'nnnn_ Cuts at: 3670 3670 Size: 6469 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2177 2202 2629 2644 2865 2942 Size: 399 289 98 25 427 15 221 77 Cuts at: 2942 3349 3573 3737 3788 4530 4615 5686 1391 Size: 407 224 164 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 407 399 289 224 221 164 98 85 77 51 25 15 BspEI T'CCGG_A Cuts at: 6132 6132 Size: 6469 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2324 2797 3824 844 Size: 1480 473 1027 3489 Fragments arranged by size: 3489 1480 1027 473 BsrBI CCG'CTC Cuts at: 1394 1823 4356 1394 Size: 429 2533 3507 Fragments arranged by size: 3507 2533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3029 4964 5138 65 Size: 1183 1781 1935 174 1396 Fragments arranged by size: 1935 1781 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5113 5300 1700 Size: 110 3303 187 2869 Fragments arranged by size: 3303 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 4529 5541 4529 Size: 1012 5457 BstAPI GCAn_nnn'nTGC Cuts at: 794 2407 2411 794 Size: 1613 4 4852 Fragments arranged by size: 4852 1613 4 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 2809 1184 Size: 1625 4844 BstXI CCAn_nnnn'nTGG Cuts at: 2740 2872 2740 Size: 132 6337 BstYI r'GATC_y Cuts at: 1972 3725 3799 4166 4551 4568 6020 6106 6118 Size: 1753 74 367 385 17 1452 86 12 Cuts at: 6118 6464 1972 Size: 346 1977 Fragments arranged by size: 1977 1753 1452 385 367 346 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2217 2500 2943 3488 513 Size: 552 939 213 283 443 545 3494 Fragments arranged by size: 3494 939 552 545 443 283 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2330 487 Size: 683 56 1104 4626 Fragments arranged by size: 4626 1104 683 56 BtsI GCAGTG_nn' Cuts at: 2405 2453 3985 4779 4799 2405 Size: 48 1532 794 20 4075 Fragments arranged by size: 4075 1532 794 48 20 ClaI AT'CG_AT Cuts at: 1554 3531 1554 Size: 1977 4492 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2668 2703 529 Size: 35 2104 35 4295 Fragments arranged by size: 4295 2104 35 35 DraI TTT'AAA Cuts at: 3773 3773 Size: 6469 DraIII CAC_nnn'GTG Cuts at: 3015 3015 Size: 6469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3323 5476 809 Size: 507 2007 2153 1802 Fragments arranged by size: 2153 2007 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2121 2310 Size: 54 1425 50 275 18 261 30 189 Cuts at: 2310 2598 2711 3030 3432 3590 3916 4805 6434 Size: 288 113 319 402 158 326 889 1629 Cuts at: 6434 8 Size: 43 Fragments arranged by size: 1629 1425 889 402 326 319 288 275 261 189 158 113 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3191 3405 4399 1353 Size: 1838 214 994 3423 Fragments arranged by size: 3423 1838 994 214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5056 5430 5576 387 Size: 1064 138 3467 374 146 1280 Fragments arranged by size: 3467 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2536 3438 4532 5129 5916 6184 Size: 393 536 368 902 1094 597 787 268 Cuts at: 6184 1239 Size: 1524 Fragments arranged by size: 1524 1094 902 787 597 536 393 368 268 EcoO109I rG'GnC_Cy Cuts at: 2697 3145 3220 3325 3346 3356 3482 3494 3627 Size: 448 75 105 21 10 126 12 133 Cuts at: 3627 3733 2697 Size: 106 5433 Fragments arranged by size: 5433 448 133 126 106 105 75 21 12 10 EcoRI G'AATT_C Cuts at: 3778 4156 3778 Size: 378 6091 FspI TGC'GCA Cuts at: 3008 4975 3008 Size: 1967 4502 FspAI rTGC'GCAy Cuts at: 3008 3008 Size: 6469 HaeII r_GCGC'y Cuts at: 1678 2087 2309 2491 2829 2909 2992 5616 1678 Size: 409 222 182 338 80 83 2624 2531 Fragments arranged by size: 2624 2531 409 338 222 182 83 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5110 5142 5184 5216 1373 Size: 32 135 32 3538 32 42 32 2626 Fragments arranged by size: 3538 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2374 3813 4059 669 Size: 988 281 436 1439 246 3079 Fragments arranged by size: 3079 1439 988 436 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6469 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KasI G'GCGC_C Cuts at: 2905 2905 Size: 6469 KpnI G_GTAC'C Cuts at: 2709 2729 3794 2709 Size: 20 1065 5384 Fragments arranged by size: 5384 1065 20 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2073 2271 2877 3369 3578 3628 5583 5767 Size: 768 198 606 492 209 50 1955 184 Cuts at: 5767 1305 Size: 2007 Fragments arranged by size: 2007 1955 768 606 492 209 198 184 50 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 2312 2600 3032 3434 Size: 54 1425 343 261 219 288 432 402 Cuts at: 3434 10 Size: 3045 Fragments arranged by size: 3045 1425 432 402 343 288 261 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2354 2429 2738 2858 2870 4427 Size: 648 1020 168 75 309 120 12 1557 Cuts at: 4427 4786 4945 518 Size: 359 159 2042 Fragments arranged by size: 2042 1557 1020 648 359 309 168 159 120 75 12 MspA1I CmG'CkG Cuts at: 1606 2195 2227 2502 3760 3885 4562 5710 5955 Size: 589 32 275 1258 125 677 1148 245 Cuts at: 5955 1606 Size: 2120 Fragments arranged by size: 2120 1258 1148 677 589 275 245 125 32 NarI GG'CG_CC Cuts at: 2906 2906 Size: 6469 NcoI C'CATG_G Cuts at: 513 1065 2004 2943 513 Size: 552 939 939 4039 Fragments arranged by size: 4039 939 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 2353 2414 3184 3827 5372 1132 Size: 97 1124 61 770 643 1545 2229 Fragments arranged by size: 2229 1545 1124 770 643 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 2100 2469 3451 1108 Size: 992 369 982 4126 Fragments arranged by size: 4126 992 982 369 PfoI T'CCnGG_A Cuts at: 3507 3507 Size: 6469 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3145 3220 3325 3356 3482 3494 3627 3145 Size: 75 105 31 126 12 133 5987 Fragments arranged by size: 5987 133 126 105 75 31 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PspOMI G'GGCC_C Cuts at: 2625 3345 3733 2625 Size: 720 388 5361 Fragments arranged by size: 5361 720 388 PstI C_TGCA'G Cuts at: 830 2808 3821 830 Size: 1978 1013 3478 Fragments arranged by size: 3478 1978 1013 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 2195 2227 3760 2195 Size: 32 1533 4904 Fragments arranged by size: 4904 1533 32 SacII CC_GC'GG Cuts at: 2503 2503 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3811 1936 Size: 1875 4594 SanDI GG'GwC_CC Cuts at: 3145 3482 3627 3145 Size: 337 145 5987 Fragments arranged by size: 5987 337 145 SapI GCTCTTCn'nnn_ Cuts at: 3191 3191 Size: 6469 SbfI CC_TGCA'GG Cuts at: 2808 3821 2808 Size: 1013 5456 ScaI AGT'ACT Cuts at: 4717 6429 4717 Size: 1712 4757 SfcI C'TryA_G Cuts at: 826 1045 2804 3817 4952 5633 5824 826 Size: 219 1759 1013 1135 681 191 1471 Fragments arranged by size: 1759 1471 1135 1013 681 219 191 SfoI GGC'GCC Cuts at: 2907 2907 Size: 6469 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2891 3197 4573 5474 5736 820 Size: 197 926 948 306 1376 901 262 1553 Fragments arranged by size: 1553 1376 948 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 2414 3827 1132 Size: 1282 1413 3774 Fragments arranged by size: 3774 1413 1282 SspI AAT'ATT Cuts at: 5 52 4393 5 Size: 47 4341 2081 Fragments arranged by size: 4341 2081 47 StuI AGG'CCT Cuts at: 1517 2036 1517 Size: 519 5950 StyI C'CwwG_G Cuts at: 513 1065 2004 2700 2943 3622 3908 513 Size: 552 939 696 243 679 286 3074 Fragments arranged by size: 3074 939 696 679 552 286 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4496 4513 4666 4851 5296 325 Size: 1255 2916 17 153 185 445 1498 Fragments arranged by size: 2916 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4715 6427 96 Size: 274 80 33 51 1443 2738 1712 138 Fragments arranged by size: 2738 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6429 6461 6429 Size: 32 6437 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6469 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2215 2215 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6469 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 4598 4598 Size: 6469 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI