(Circular) (Six-base) MAPSORT of: pFN21AB9510.seq Check: 201 from: 1 to: 5821 pFN21AB9510 5821 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 3884 5268 5536 1632 Size: 483 1769 1384 268 1917 Fragments arranged by size: 1917 1769 1384 483 268 AflII C'TTAA_G Cuts at: 820 1017 2810 820 Size: 197 1793 3831 Fragments arranged by size: 3831 1793 197 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 2113 1166 Size: 947 4874 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AlwNI CAG_nnn'CTG Cuts at: 2408 2837 3564 5136 2408 Size: 429 727 1572 3093 Fragments arranged by size: 3093 1572 727 429 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 3146 1870 Size: 73 1203 4545 Fragments arranged by size: 4545 1203 73 AvrII C'CTAG_G Cuts at: 2138 2138 Size: 5821 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3044 3142 4597 618 Size: 325 217 627 1257 98 1455 1842 Fragments arranged by size: 1842 1455 1257 627 325 217 98 BanII G_rGCy'C Cuts at: 1391 3140 1391 Size: 1749 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4850 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 2072 2346 3238 2072 Size: 274 892 4655 Fragments arranged by size: 4655 892 274 Bme1580I G_kGCm'C Cuts at: 1790 3882 5038 1790 Size: 2092 1156 2573 Fragments arranged by size: 2573 2092 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3552 4510 442 Size: 416 53 83 399 24 2135 958 1753 Fragments arranged by size: 2135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2670 3088 4481 1239 Size: 1431 418 1393 2579 Fragments arranged by size: 2579 1431 1393 418 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2115 2764 3612 493 Size: 711 911 649 848 2702 Fragments arranged by size: 2702 911 848 711 649 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3628 4010 4645 275 Size: 53 83 186 936 2095 382 635 1451 Fragments arranged by size: 2095 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5821 BseYI C'CCAG_C Cuts at: 2240 5024 5520 2240 Size: 2784 496 2541 Fragments arranged by size: 2784 2541 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5248 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3882 3967 5038 3882 Size: 85 1071 4665 Fragments arranged by size: 4665 1071 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3140 3882 3967 5038 1391 Size: 399 1350 742 85 1071 2174 Fragments arranged by size: 2174 1350 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2407 3176 844 Size: 1563 769 3489 Fragments arranged by size: 3489 1563 769 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2787 4316 4490 65 Size: 1183 1539 1529 174 1396 Fragments arranged by size: 1539 1529 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4465 4652 1700 Size: 110 2655 187 2869 Fragments arranged by size: 2869 2655 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 2975 794 Size: 2181 3640 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstXI CCAn_nnnn'nTGG Cuts at: 2184 2184 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2199 3151 3518 3903 3920 5372 5458 5470 Size: 227 952 367 385 17 1452 86 12 Cuts at: 5470 5816 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 952 385 367 346 227 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2457 2457 Size: 5821 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5269 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2382 3337 4131 4151 2382 Size: 955 794 20 4052 Fragments arranged by size: 4052 955 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2704 2739 529 Size: 35 2140 35 3611 Fragments arranged by size: 3611 2140 35 35 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3268 4157 5786 Size: 54 1425 50 275 18 1438 889 1629 Cuts at: 5786 8 Size: 43 Fragments arranged by size: 1629 1438 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2556 3751 1353 Size: 1203 1195 3423 Fragments arranged by size: 3423 1203 1195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2670 3088 3884 4481 5268 5536 Size: 393 483 555 418 796 597 787 268 Cuts at: 5536 1239 Size: 1524 Fragments arranged by size: 1524 796 787 597 555 483 418 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2087 2087 Size: 5821 EcoO109I rG'GnC_Cy Cuts at: 2362 2362 Size: 5821 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 4968 1678 Size: 3290 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4462 4494 4536 4568 1373 Size: 32 135 32 2890 32 42 32 2626 Fragments arranged by size: 2890 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2484 3165 3411 669 Size: 988 281 72 474 681 246 3079 Fragments arranged by size: 3079 988 681 474 281 246 72 HindIII A'AGCT_T Cuts at: 748 3094 748 Size: 2346 3475 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4935 5119 1305 Size: 3630 184 2007 Fragments arranged by size: 3630 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2113 2766 2868 3779 4138 4297 518 Size: 648 947 653 102 911 359 159 2042 Fragments arranged by size: 2042 947 911 653 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2015 2350 2408 3237 3914 5062 5307 1606 Size: 409 335 58 829 677 1148 245 2120 Fragments arranged by size: 2120 1148 829 677 409 335 245 58 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5269 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PmlI CAC'GTG Cuts at: 2115 2115 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2362 Size: 5821 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2979 3011 2979 Size: 32 5789 PstI C_TGCA'G Cuts at: 830 2091 2404 3028 3173 830 Size: 1261 313 624 145 3478 Fragments arranged by size: 3478 1261 624 313 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2015 2350 2408 2015 Size: 335 58 5428 Fragments arranged by size: 5428 335 58 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 3022 4069 5781 3022 Size: 1047 1712 3062 Fragments arranged by size: 3062 1712 1047 SexAI A'CCwGG_T Cuts at: 2049 2049 Size: 5821 SfcI C'TryA_G Cuts at: 826 1045 2087 2400 3024 3169 4304 4985 5176 Size: 219 1042 313 624 145 1135 681 191 Cuts at: 5176 826 Size: 1471 Fragments arranged by size: 1471 1135 1042 681 624 313 219 191 145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2810 3925 4826 5088 820 Size: 197 926 867 1115 901 262 1553 Fragments arranged by size: 1553 1115 926 901 867 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2138 2487 3260 513 Size: 552 1073 349 773 3074 Fragments arranged by size: 3074 1073 773 552 349 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2125 2254 3848 3865 4018 4203 4648 Size: 1255 545 129 1594 17 153 185 445 Cuts at: 4648 325 Size: 1498 Fragments arranged by size: 1594 1498 1255 545 445 185 153 129 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2367 3020 4067 Size: 274 80 33 51 1443 390 653 1047 Cuts at: 4067 5779 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1047 653 390 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5781 5813 5781 Size: 32 5789 Tth111I GACn'n_nGTC Cuts at: 1316 2939 1316 Size: 1623 4198 XbaI T'CTAG_A Cuts at: 2497 3157 2497 Size: 660 5161 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2275 2275 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI