(Circular) (Six-base) MAPSORT of: pFN21AB9451.seq Check: 5525 from: 1 to: 5701 pFN21AB9451 5701 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3044 1937 Size: 1107 4594 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3764 5148 5416 1632 Size: 2132 1384 268 1917 Fragments arranged by size: 2132 1917 1384 268 AfeI AGC'GCT Cuts at: 2474 2474 Size: 5701 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 4600 5577 1203 Size: 3397 977 1327 Fragments arranged by size: 3397 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5701 AlwNI CAG_nnn'CTG Cuts at: 3444 5016 3444 Size: 1572 4129 ApaI G_GGCC'C Cuts at: 2865 2865 Size: 5701 ApaLI G'TGCA_C Cuts at: 3758 4914 3758 Size: 1156 4545 ApoI r'AATT_y Cuts at: 1493 3010 3236 3388 5497 1493 Size: 1517 226 152 2109 1697 Fragments arranged by size: 2109 1697 1517 226 152 AscI GG'CGCG_CC Cuts at: 2868 2868 Size: 5701 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 2291 2960 3026 1870 Size: 73 348 669 66 4545 Fragments arranged by size: 4545 669 348 73 66 BamHI G'GATC_C Cuts at: 3031 3398 3031 Size: 367 5334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2162 2560 2653 3022 Size: 325 217 627 222 153 398 93 369 Cuts at: 3022 4477 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 398 369 325 222 217 153 93 BanII G_rGCy'C Cuts at: 1391 2220 2352 2787 2865 2907 3020 1391 Size: 829 132 435 78 42 113 4072 Fragments arranged by size: 4072 829 435 132 113 78 42 BbeI G_GCGC'C Cuts at: 2166 2564 2657 2166 Size: 398 93 5210 Fragments arranged by size: 5210 398 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4730 BbvCI CC'TCA_GC Cuts at: 2556 2556 Size: 5701 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3892 3926 1903 Size: 34 1955 34 3678 Fragments arranged by size: 3678 1955 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 1548 Size: 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2294 2387 2621 Size: 107 122 71 631 605 622 93 234 Cuts at: 2621 2816 2843 4312 136 Size: 195 27 1469 1525 Fragments arranged by size: 1525 1469 631 622 605 234 195 122 107 93 71 27 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 2501 3118 2501 Size: 617 5084 Bme1580I G_kGCm'C Cuts at: 1790 2014 2865 3762 4918 1790 Size: 224 851 897 1156 2573 Fragments arranged by size: 2573 1156 897 851 224 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2008 3432 4390 Size: 416 53 83 399 24 591 1424 958 Cuts at: 4390 442 Size: 1753 Fragments arranged by size: 1753 1424 958 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2371 2866 2371 Size: 495 5206 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2559 4361 1239 Size: 1320 1802 2579 Fragments arranged by size: 2579 1802 1320 Bpu10I CC'TnA_GC Cuts at: 2556 2556 Size: 5701 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2444 2759 4364 882 Size: 617 945 315 1605 2219 Fragments arranged by size: 2219 1605 945 617 315 BsaAI yAC'GTr Cuts at: 493 1204 2119 3492 493 Size: 711 915 1373 2702 Fragments arranged by size: 2702 1373 915 711 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 2243 2561 2654 Size: 53 83 186 936 630 80 318 93 Cuts at: 2654 3508 3890 4525 275 Size: 854 382 635 1451 Fragments arranged by size: 1451 936 854 635 630 382 318 186 93 83 80 53 BsaWI w'CCGG_w Cuts at: 4134 4532 4806 4953 5364 4134 Size: 398 274 147 411 4471 Fragments arranged by size: 4471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5476 Fragments arranged by size: 5476 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2228 2386 2678 2696 2795 2813 2828 Size: 1000 42 158 292 18 99 18 15 Cuts at: 2828 2915 1186 Size: 87 3972 Fragments arranged by size: 3972 1000 292 158 99 87 42 18 18 15 BseYI C'CCAG_C Cuts at: 2441 2786 2955 2983 4904 5400 2441 Size: 345 169 28 1921 496 2742 Fragments arranged by size: 2742 1921 496 345 169 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2986 1163 Size: 573 1250 3878 Fragments arranged by size: 3878 1250 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2151 2202 2226 2259 2277 2367 Size: 275 186 150 51 24 33 18 90 Cuts at: 2367 2391 2613 2913 3151 3912 4940 1540 Size: 24 222 300 238 761 1028 2301 Fragments arranged by size: 2301 1028 761 300 275 238 222 186 150 90 51 33 24 24 18 BsiHKAI G_wGCw'C Cuts at: 3762 3847 4918 3762 Size: 85 1071 4545 Fragments arranged by size: 4545 1071 85 BsmI GAATG_Cn' Cuts at: 3212 3305 3212 Size: 93 5608 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2220 2352 2787 2865 2907 3020 Size: 399 224 206 132 435 78 42 113 Cuts at: 3020 3762 3847 4918 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 224 206 132 113 85 78 42 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 3590 5320 3590 Size: 1730 3971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3056 844 Size: 2212 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2235 2505 3588 1394 Size: 429 412 270 1083 3507 Fragments arranged by size: 3507 1083 429 412 270 BsrDI GCAATG_nn' Cuts at: 65 1248 4196 4370 65 Size: 1183 2948 174 1396 Fragments arranged by size: 2948 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2272 4345 4532 1700 Size: 110 462 2073 187 2869 Fragments arranged by size: 2869 2073 462 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5701 BssHII G'CGCG_C Cuts at: 1925 2868 1925 Size: 943 4758 BssSI C'ACGA_G Cuts at: 3761 4773 3761 Size: 1012 4689 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5701 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5701 BstXI CCAn_nnnn'nTGG Cuts at: 2794 2794 Size: 5701 BstYI r'GATC_y Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696 Size: 1059 367 385 17 1452 86 12 346 Cuts at: 5696 1972 Size: 1977 Fragments arranged by size: 1977 1452 1059 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 2145 2220 2253 2328 2361 Size: 552 939 45 96 75 33 75 33 Cuts at: 2361 2367 2895 2907 513 Size: 6 528 12 3307 Fragments arranged by size: 3307 939 552 528 96 75 75 45 33 33 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4962 Fragments arranged by size: 4962 683 56 BtsI GCAGTG_nn' Cuts at: 3217 4011 4031 3217 Size: 794 20 4887 Fragments arranged by size: 4887 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5666 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4708 809 Size: 507 3392 1802 Fragments arranged by size: 3392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 2148 2199 Size: 54 1425 50 275 18 283 35 51 Cuts at: 2199 2223 2256 2274 2364 2388 2610 2910 3148 Size: 24 33 18 90 24 222 300 238 Cuts at: 3148 4037 5666 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 300 283 275 238 222 90 54 51 50 43 35 33 24 24 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2148 2199 2223 2256 2274 2364 2388 Size: 275 336 51 24 33 18 90 24 Cuts at: 2388 2610 2910 3148 1537 Size: 222 300 238 4090 Fragments arranged by size: 4090 336 300 275 238 222 90 51 33 24 24 18 EarI CTCTTCn'nnn_ Cuts at: 1353 3631 1353 Size: 2278 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2842 4288 4662 4808 387 Size: 1064 138 1253 1446 374 146 1280 Fragments arranged by size: 1446 1280 1253 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2559 3764 4361 5148 5416 1239 Size: 393 927 1205 597 787 268 1524 Fragments arranged by size: 1524 1205 927 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2323 2845 2323 Size: 522 5179 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2491 2491 Size: 5701 FspI TGC'GCA Cuts at: 2935 4207 2935 Size: 1272 4429 HaeII r_GCGC'y Cuts at: 1678 2166 2196 2476 2564 2657 2832 4848 1678 Size: 488 30 280 88 93 175 2016 2531 Fragments arranged by size: 2531 2016 488 280 175 93 88 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 4342 4374 4416 Size: 32 135 32 797 32 1941 32 42 Cuts at: 4416 4448 1373 Size: 32 2626 Fragments arranged by size: 2626 1941 797 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3045 3291 669 Size: 988 281 1107 246 3079 Fragments arranged by size: 3079 1107 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KasI G'GCGC_C Cuts at: 2162 2560 2653 2162 Size: 398 93 5210 Fragments arranged by size: 5210 398 93 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4815 4999 1305 Size: 3510 184 2007 Fragments arranged by size: 3510 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2115 10 Size: 54 1425 343 283 3596 Fragments arranged by size: 3596 1425 343 283 54 MslI CAynn'nnrTG Cuts at: 518 1166 3659 4018 4177 518 Size: 648 2493 359 159 2042 Fragments arranged by size: 2493 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2147 2222 2255 2285 2330 2369 2567 Size: 445 96 75 33 30 45 39 198 Cuts at: 2567 2606 2651 2675 2732 2897 2909 2987 3117 Size: 39 45 24 57 165 12 78 130 Cuts at: 3117 3794 4942 5187 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 445 245 198 165 130 96 78 75 57 45 45 39 39 33 30 24 12 NaeI GCC'GGC Cuts at: 2274 2274 Size: 5701 NarI GG'CG_CC Cuts at: 2163 2561 2654 2163 Size: 398 93 5210 Fragments arranged by size: 5210 398 93 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4210 Fragments arranged by size: 4210 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NgoMIV G'CCGG_C Cuts at: 2272 2272 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 2148 2223 2610 2910 3148 2148 Size: 75 387 300 238 4701 Fragments arranged by size: 4701 387 300 238 75 NspI r_CATG'y Cuts at: 1132 1229 3059 4604 1132 Size: 97 1830 1545 2229 Fragments arranged by size: 2229 1830 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5701 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5701 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PmlI CAC'GTG Cuts at: 2119 2119 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 3271 3271 Size: 5701 PspOMI G'GGCC_C Cuts at: 2861 2861 Size: 5701 PstI C_TGCA'G Cuts at: 830 2181 3053 830 Size: 1351 872 3478 Fragments arranged by size: 3478 1351 872 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 PvuII CAG'CTG Cuts at: 2606 2606 Size: 5701 SacII CC_GC'GG Cuts at: 2052 2148 2223 2256 2331 2370 2898 2910 2052 Size: 96 75 33 75 39 528 12 4843 Fragments arranged by size: 4843 528 96 75 75 39 33 12 SalI G'TCGA_C Cuts at: 1936 3043 1936 Size: 1107 4594 SbfI CC_TGCA'GG Cuts at: 3053 3053 Size: 5701 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SfcI C'TryA_G Cuts at: 826 1045 2177 3049 4184 4865 5056 826 Size: 219 1132 872 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 872 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2387 2816 2387 Size: 429 5272 SfoI GGC'GCC Cuts at: 2164 2562 2655 2164 Size: 398 93 5210 Fragments arranged by size: 5210 398 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3805 4706 4968 820 Size: 197 926 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 3059 1132 Size: 1927 3774 SspI AAT'ATT Cuts at: 5 52 3625 5 Size: 47 3573 2081 Fragments arranged by size: 3573 2081 47 StuI AGG'CCT Cuts at: 1517 2059 1517 Size: 542 5159 StyI C'CwwG_G Cuts at: 513 1065 2004 2166 2412 2769 3140 513 Size: 552 939 162 246 357 371 3074 Fragments arranged by size: 3074 939 552 371 357 246 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 3728 3745 3898 4083 4528 325 Size: 1255 417 1731 17 153 185 445 1498 Fragments arranged by size: 1731 1498 1255 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3947 5659 96 Size: 274 80 33 51 1443 1970 1712 138 Fragments arranged by size: 1970 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5661 5693 5661 Size: 32 5669 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5701 XbaI T'CTAG_A Cuts at: 3037 3037 Size: 5701 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5701 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 2492 3830 2492 Size: 1338 4363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI