(Circular) (Six-base) MAPSORT of: pFN21AB9385.seq Check: 5561 from: 1 to: 5677 pFN21AB9385 5677 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2826 2998 2826 Size: 172 5505 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2839 2946 3740 5124 5392 1632 Size: 1207 107 794 1384 268 1917 Fragments arranged by size: 1917 1384 1207 794 268 107 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 2112 4576 5553 1203 Size: 909 2464 977 1327 Fragments arranged by size: 2464 1327 977 909 AgeI A'CCGG_T Cuts at: 2009 4508 2009 Size: 2499 3178 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 2117 1166 Size: 951 4726 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 2092 2441 3420 4992 2092 Size: 349 979 1572 2777 Fragments arranged by size: 2777 1572 979 349 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2986 3212 3364 5473 1493 Size: 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2042 3002 1870 Size: 73 99 960 4545 Fragments arranged by size: 4545 960 99 73 BamHI G'GATC_C Cuts at: 2374 2788 3007 3374 2374 Size: 414 219 367 4677 Fragments arranged by size: 4677 414 367 219 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2826 2998 4453 618 Size: 325 217 627 387 652 172 1455 1842 Fragments arranged by size: 1842 1455 652 627 387 325 217 172 BanII G_rGCy'C Cuts at: 1391 2996 1391 Size: 1605 4072 BbeI G_GCGC'C Cuts at: 2178 2178 Size: 5677 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 2263 1548 Size: 715 4962 BfrBI ATG'CAT Cuts at: 2248 2248 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 3738 4894 1790 Size: 1948 1156 2573 Fragments arranged by size: 2573 1948 1156 BmgBI CAC'GTC Cuts at: 1318 2115 1318 Size: 797 4880 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2080 2705 3408 Size: 416 53 83 399 24 663 625 703 Cuts at: 3408 4366 442 Size: 958 1753 Fragments arranged by size: 1753 958 703 663 625 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2236 2463 4337 1239 Size: 997 227 1874 2579 Fragments arranged by size: 2579 1874 997 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 3802 4667 4965 2141 Size: 1661 865 298 2853 Fragments arranged by size: 2853 1661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 4340 882 Size: 617 940 1901 2219 Fragments arranged by size: 2219 1901 940 617 BsaAI yAC'GTr Cuts at: 493 1204 2512 3468 493 Size: 711 1308 956 2702 Fragments arranged by size: 2702 1308 956 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 3484 3866 4501 Size: 53 83 186 936 642 1309 382 635 Cuts at: 4501 275 Size: 1451 Fragments arranged by size: 1451 1309 936 642 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2009 4110 4508 4782 4929 5340 2009 Size: 2101 398 274 147 411 2346 Fragments arranged by size: 2346 2101 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5452 Fragments arranged by size: 5452 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 2092 1186 Size: 861 45 4771 Fragments arranged by size: 4771 861 45 BseYI C'CCAG_C Cuts at: 2396 4880 5376 2396 Size: 2484 496 2697 Fragments arranged by size: 2697 2484 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2534 1163 Size: 573 798 4306 Fragments arranged by size: 4306 798 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3738 3823 4894 3738 Size: 85 1071 4521 Fragments arranged by size: 4521 1071 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 BsmBI CGTCTCn'nnnn_ Cuts at: 2129 2129 Size: 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2996 3738 3823 4894 1391 Size: 399 1206 742 85 1071 2174 Fragments arranged by size: 2174 1206 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4172 4346 65 Size: 1183 2924 174 1396 Fragments arranged by size: 2924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2137 2171 2448 4321 4508 1700 Size: 110 199 128 34 277 1873 187 2869 Fragments arranged by size: 2869 1873 277 199 187 128 110 34 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 2833 3737 4749 2833 Size: 904 1012 3761 Fragments arranged by size: 3761 1012 904 BstAPI GCAn_nnn'nTGC Cuts at: 794 2849 794 Size: 2055 3622 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstYI r'GATC_y Cuts at: 1972 2374 2788 3007 3374 3759 3776 5228 5314 Size: 402 414 219 367 385 17 1452 86 Cuts at: 5314 5326 5672 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 414 402 385 367 346 219 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2640 2640 Size: 5677 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2824 487 Size: 683 56 1598 3340 Fragments arranged by size: 3340 1598 683 56 BtsI GCAGTG_nn' Cuts at: 3193 3987 4007 3193 Size: 794 20 4863 Fragments arranged by size: 4863 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 3124 4013 Size: 54 1425 50 275 18 309 985 889 Cuts at: 4013 5642 8 Size: 1629 43 Fragments arranged by size: 1629 1425 985 889 309 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3607 1353 Size: 2254 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2236 2463 2839 2946 3740 4337 5124 Size: 393 604 227 376 107 794 597 787 Cuts at: 5124 5392 1239 Size: 268 1524 Fragments arranged by size: 1524 794 787 604 597 393 376 268 227 107 EcoO109I rG'GnC_Cy Cuts at: 2283 2400 2283 Size: 117 5560 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2291 2680 2291 Size: 389 5288 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2178 4824 1678 Size: 500 2646 2531 Fragments arranged by size: 2646 2531 500 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2392 2424 4318 Size: 32 135 32 467 32 321 32 1894 Cuts at: 4318 4350 4392 4424 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1894 467 321 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KasI G'GCGC_C Cuts at: 2174 2174 Size: 5677 KpnI G_GTAC'C Cuts at: 2830 3002 2830 Size: 172 5505 MfeI C'AATT_G Cuts at: 2237 3276 2237 Size: 1039 4638 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2228 4791 4975 1305 Size: 923 2563 184 2007 Fragments arranged by size: 2563 2007 923 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3855 Fragments arranged by size: 3855 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 3635 3994 4153 518 Size: 648 951 1518 359 159 2042 Fragments arranged by size: 2042 1518 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3093 3770 4918 5163 1606 Size: 1487 677 1148 245 2120 Fragments arranged by size: 2120 1487 1148 677 245 NaeI GCC'GGC Cuts at: 2139 2139 Size: 5677 NarI GG'CG_CC Cuts at: 2175 2175 Size: 5677 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5125 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NgoMIV G'CCGG_C Cuts at: 2137 2137 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NsiI A_TGCA'T Cuts at: 2250 2250 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2213 3035 4580 1132 Size: 97 984 822 1545 2229 Fragments arranged by size: 2229 1545 984 822 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5677 PfoI T'CCnGG_A Cuts at: 2181 2181 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2283 2400 2283 Size: 117 5560 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2492 2524 2492 Size: 32 5645 PstI C_TGCA'G Cuts at: 830 3029 830 Size: 2199 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SanDI GG'GwC_CC Cuts at: 2283 2283 Size: 5677 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SfcI C'TryA_G Cuts at: 826 1045 3025 4160 4841 5032 826 Size: 219 1980 1135 681 191 1471 Fragments arranged by size: 1980 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2176 2176 Size: 5677 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 2171 1700 Size: 471 5206 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2156 3781 4682 4944 820 Size: 197 926 213 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 262 213 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 2213 3035 1132 Size: 1081 822 3774 Fragments arranged by size: 3774 1081 822 SspI AAT'ATT Cuts at: 5 52 2695 2782 3601 5 Size: 47 2643 87 819 2081 Fragments arranged by size: 2643 2081 819 87 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2973 3116 513 Size: 552 1908 143 3074 Fragments arranged by size: 3074 1908 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3704 3721 3874 4059 4504 325 Size: 1255 2124 17 153 185 445 1498 Fragments arranged by size: 2124 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3923 5635 96 Size: 274 80 33 51 1443 1946 1712 138 Fragments arranged by size: 1946 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2905 2937 5637 5669 2905 Size: 32 2700 32 2913 Fragments arranged by size: 2913 2700 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2211 2211 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI