Restriction map for
pFN21AB9100
(Circular) (Six-base) MAPSORT of: pFN21AB9100.seq Check: 5058 from: 1 to: 8029
pFN21AB9100 8029 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3984 3984
Size: 8029
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5839 278
Size: 53 83 186 936 4303 2468
Fragments arranged by size:
4303 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2022 5009 5278 5372 1937
Size: 85 2987 269 94 4594
Fragments arranged by size:
4594 2987 269 94 85
Acc65I G'GTAC_C
Cuts at: 5350 5350
Size: 8029
AclI AA'CG_TT
Cuts at: 1563 6156 6529 1563
Size: 4593 373 3063
Fragments arranged by size:
4593 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 3443 4460 4661 6092 7476 7744 1632
Size: 509 1302 1017 201 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 1302 1017 509 268 201
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7832
AflIII A'CryG_T
Cuts at: 1203 3399 6928 7905 1203
Size: 2196 3529 977 1327
Fragments arranged by size:
3529 2196 1327 977
AgeI A'CCGG_T
Cuts at: 2955 6860 2955
Size: 3905 4124
AhdI GACnn_n'nnGTC
Cuts at: 5026 6758 5026
Size: 1732 6297
AleI CACnn'nnGTG
Cuts at: 1166 2061 1166
Size: 895 7134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3635 1184
Size: 2451 5578
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 2486
Size: 32 7997
AlwNI CAG_nnn'CTG
Cuts at: 2366 3445 3795 5299 5772 7344 2366
Size: 1079 350 1504 473 1572 3051
Fragments arranged by size:
3051 1572 1504 1079 473 350
ApaI G_GGCC'C
Cuts at: 4412 4667 4412
Size: 255 7774
ApaLI G'TGCA_C
Cuts at: 6086 7242 6086
Size: 1156 6873
ApoI r'AATT_y
Cuts at: 1493 3896 4127 4559 4619 5338 5564 5716 7825
Size: 2403 231 432 60 719 226 152 2109
Cuts at: 7825 1493
Size: 1697
Fragments arranged by size:
2403 2109 1697 719 432 231 226 152
60
AseI AT'TA_AT
Cuts at: 160 1032 2171 6583 160
Size: 872 1139 4412 1606
Fragments arranged by size:
4412 1606 1139 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8029
AvaI C'yCGr_G
Cuts at: 1870 1943 2725 3057 3087 4046 4101 5354 1870
Size: 73 782 332 30 959 55 1253 4545
Fragments arranged by size:
4545 1253 959 782 332 73 55 30
BamHI G'GATC_C
Cuts at: 5359 5726 5359
Size: 367 7662
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2657 3672 5161 5350 6805
Size: 325 217 627 870 1015 1489 189 1455
Cuts at: 6805 618
Size: 1842
Fragments arranged by size:
1842 1489 1455 1015 870 627 325 217
189
BanII G_rGCy'C
Cuts at: 1391 2732 4412 4667 4685 5348 1391
Size: 1341 1680 255 18 663 4072
Fragments arranged by size:
4072 1680 1341 663 255 18
BbeI G_GCGC'C
Cuts at: 2661 3676 2661
Size: 1015 7014
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4540 4601 928
Size: 971 2641 61 4356
Fragments arranged by size:
4356 2641 971 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6220 6254 1903
Size: 34 4283 34 3678
Fragments arranged by size:
4283 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3547 4877 5921 7131 3547
Size: 1330 1044 1210 4445
Fragments arranged by size:
4445 1330 1210 1044
BclI T'GATC_A
Cuts at: 1548 2301 1548
Size: 753 7276
BfrBI ATG'CAT
Cuts at: 3415 3415
Size: 8029
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2727 3943 6640
Size: 107 122 71 631 605 1055 1216 2697
Cuts at: 6640 136
Size: 1525
Fragments arranged by size:
2697 1525 1216 1055 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2568 8024 2568
Size: 5456 2573
BlpI GC'TnA_GC
Cuts at: 5446 5446
Size: 8029
Bme1580I G_kGCm'C
Cuts at: 1790 4412 4667 6090 7246 1790
Size: 2622 255 1423 1156 2573
Fragments arranged by size:
2622 2573 1423 1156 255
BmgBI CAC'GTC
Cuts at: 1318 3400 1318
Size: 2082 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3805 4669 4731
Size: 416 53 83 399 24 2388 864 62
Cuts at: 4731 4850 5760 6718 442
Size: 119 910 958 1753
Fragments arranged by size:
2388 1753 958 910 864 416 399 119
83 62 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8029
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 4373 2677
Size: 1696 6333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2377 2424 2483 2488 3434 4250 4690 6689
Size: 1138 47 59 5 946 816 440 1999
Cuts at: 6689 1239
Size: 2579
Fragments arranged by size:
2579 1999 1138 946 816 440 59 47
5
Bpu10I CC'TnA_GC
Cuts at: 2717 4565 5196 2717
Size: 1848 631 5550
Fragments arranged by size:
5550 1848 631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4715 5042 6154 7019 7317 4715
Size: 327 1112 865 298 5427
Fragments arranged by size:
5427 1112 865 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4344 6692 882
Size: 617 2845 2348 2219
Fragments arranged by size:
2845 2348 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2428 3025 5820 493
Size: 711 1224 597 2795 2702
Fragments arranged by size:
2795 2702 1224 711 597
BsaBI GATnn'nnATC
Cuts at: 2801 8023 2801
Size: 5222 2807
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2658 3159 3673 3966
Size: 53 83 186 936 1125 501 514 293
Cuts at: 3966 5036 5836 6218 6853 275
Size: 1070 800 382 635 1451
Fragments arranged by size:
1451 1125 1070 936 800 635 514 501
382 293 186 83 53
BsaWI w'CCGG_w
Cuts at: 2490 2955 3643 6462 6860 7134 7281 7692 2490
Size: 465 688 2819 398 274 147 411 2827
Fragments arranged by size:
2827 2819 688 465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 4195 4225 1178
Size: 30 165 30 1083 30 1679 30 4982
Fragments arranged by size:
4982 1679 1083 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2421 3968 4036 4604 4863 1186
Size: 1235 1547 68 568 259 4352
Fragments arranged by size:
4352 1547 1235 568 259 68
BseYI C'CCAG_C
Cuts at: 3527 4015 5116 5139 7232 7728 3527
Size: 488 1101 23 2093 496 3828
Fragments arranged by size:
3828 2093 1101 496 488 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2765 3065 3956 4695 4715 1163
Size: 573 1029 300 891 739 20 4477
Fragments arranged by size:
4477 1029 891 739 573 300 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5479 6240 7268 1540
Size: 275 186 3478 761 1028 2301
Fragments arranged by size:
3478 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3486 3728 4257 6090 6175 7246 3486
Size: 242 529 1833 85 1071 4269
Fragments arranged by size:
4269 1833 1071 529 242 85
BsmI GAATG_Cn'
Cuts at: 5540 5633 5540
Size: 93 7936
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2732 3486 3728 4257 4412 4667 4685
Size: 399 942 754 242 529 155 255 18
Cuts at: 4685 5348 6090 6175 7246 1391
Size: 663 742 85 1071 2174
Fragments arranged by size:
2174 1071 942 754 742 663 529 399
255 242 155 85 18
BspEI T'CCGG_A
Cuts at: 2490 3643 7692 2490
Size: 1153 4049 2827
Fragments arranged by size:
4049 2827 1153
BspHI T'CATG_A
Cuts at: 2379 3381 3478 5918 7648 2379
Size: 1002 97 2440 1730 2760
Fragments arranged by size:
2760 2440 1730 1002 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3984 4362 4744 5384 844
Size: 3140 378 382 640 3489
Fragments arranged by size:
3489 3140 640 382 378
BsrBI CCG'CTC
Cuts at: 1394 1823 2240 2623 5916 1394
Size: 429 417 383 3293 3507
Fragments arranged by size:
3507 3293 429 417 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 6524 6698 65
Size: 1183 5276 174 1396
Fragments arranged by size:
5276 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2955 2979 6673 6860 1700
Size: 110 1145 24 3694 187 2869
Fragments arranged by size:
3694 2869 1145 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 2289 4968 96
Size: 2193 2679 3157
Fragments arranged by size:
3157 2679 2193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8029
BssSI C'ACGA_G
Cuts at: 6089 7101 6089
Size: 1012 7017
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3979 794
Size: 3185 4844
BstBI TT'CG_AA
Cuts at: 6833 6833
Size: 8029
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8029
BstXI CCAn_nnnn'nTGG
Cuts at: 3197 4712 4937 3197
Size: 1515 225 6289
Fragments arranged by size:
6289 1515 225
BstYI r'GATC_y
Cuts at: 1972 2162 2568 2802 3589 4464 5254 5359 5726
Size: 190 406 234 787 875 790 105 367
Cuts at: 5726 6111 6128 7580 7666 7678 8024 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 875 790 787 406 385 367
346 234 190 105 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5157 5157
Size: 8029
BtgI C'CryG_G
Cuts at: 513 1065 2004 3126 4977 513
Size: 552 939 1122 1851 3565
Fragments arranged by size:
3565 1851 1122 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7290
Fragments arranged by size:
7290 683 56
BtsI GCAGTG_nn'
Cuts at: 5545 6339 6359 5545
Size: 794 20 7215
Fragments arranged by size:
7215 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8029
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2041 2076 529
Size: 35 1477 35 6482
Fragments arranged by size:
6482 1477 35 35
DraI TTT'AAA
Cuts at: 5333 5333
Size: 8029
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4724 7036 809
Size: 507 3408 2312 1802
Fragments arranged by size:
3408 2312 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5476 6365 7994
Size: 54 1425 50 275 18 3646 889 1629
Cuts at: 7994 8
Size: 43
Fragments arranged by size:
3646 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5476 1537
Size: 275 3664 4090
Fragments arranged by size:
4090 3664 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2808 2904 3856 4062 4245 5959 1353
Size: 767 688 96 952 206 183 1714 3423
Fragments arranged by size:
3423 1714 952 767 688 206 183 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6616 6990 7136 387
Size: 1064 138 5027 374 146 1280
Fragments arranged by size:
5027 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2377 2424 2483 2488 3434 3443
Size: 393 509 236 47 59 5 946 9
Cuts at: 3443 4250 4460 4661 4690 6092 6689 7476 7744
Size: 807 210 201 29 1402 597 787 268
Cuts at: 7744 1239
Size: 1524
Fragments arranged by size:
1524 1402 946 807 787 597 509 393
268 236 210 201 59 47 29 9
5
EcoNI CCTnn'n_nnAGG
Cuts at: 4878 4878
Size: 8029
EcoO109I rG'GnC_Cy
Cuts at: 3076 4408 4420 4663 4664 4717 5062 5296 3076
Size: 1332 12 243 1 53 345 234 5809
Fragments arranged by size:
5809 1332 345 243 234 53 12 1
EcoRI G'AATT_C
Cuts at: 4559 5338 5716 4559
Size: 779 378 6872
Fragments arranged by size:
6872 779 378
FspI TGC'GCA
Cuts at: 6535 6535
Size: 8029
HaeII r_GCGC'y
Cuts at: 1678 2661 3676 7176 1678
Size: 983 1015 3500 2531
Fragments arranged by size:
3500 2531 1015 983
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3460 3492 4383 4415 4939
Size: 32 135 32 1888 32 891 32 524
Cuts at: 4939 4971 5012 5044 6670 6702 6744 6776 1373
Size: 32 41 32 1626 32 42 32 2626
Fragments arranged by size:
2626 1888 1626 891 524 135 42 41
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 3991 5279 5373 5619 669
Size: 988 281 244 1809 1288 94 246 3079
Fragments arranged by size:
3079 1809 1288 988 281 246 244 94
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8029
HpaI GTT'AAC
Cuts at: 5619 5619
Size: 8029
KasI G'GCGC_C
Cuts at: 2657 3672 2657
Size: 1015 7014
KpnI G_GTAC'C
Cuts at: 5354 5354
Size: 8029
MfeI C'AATT_G
Cuts at: 5628 5628
Size: 8029
MluI A'CGCG_T
Cuts at: 7905 7905
Size: 8029
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 2598 3476 7143 7327 1305
Size: 1170 123 878 3667 184 2007
Fragments arranged by size:
3667 2007 1170 878 184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6207
Fragments arranged by size:
6207 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 2268 2696 2792 3023 3522 3731
Size: 648 895 207 428 96 231 499 209
Cuts at: 3731 3854 4976 5987 6346 6505 518
Size: 123 1122 1011 359 159 2042
Fragments arranged by size:
2042 1122 1011 895 648 499 428 359
231 209 207 159 123 96
MspA1I CmG'CkG
Cuts at: 1606 2458 4015 5445 6122 7270 7515 1606
Size: 852 1557 1430 677 1148 245 2120
Fragments arranged by size:
2120 1557 1430 1148 852 677 245
NaeI GCC'GGC
Cuts at: 2981 2981
Size: 8029
NarI GG'CG_CC
Cuts at: 2658 3673 2658
Size: 1015 7014
NcoI C'CATG_G
Cuts at: 513 1065 2004 3126 4977 513
Size: 552 939 1122 1851 3565
Fragments arranged by size:
3565 1851 1122 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8029
NgoMIV G'CCGG_C
Cuts at: 2979 2979
Size: 8029
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8029
NotI GC'GGCC_GC
Cuts at: 5476 5476
Size: 8029
NsiI A_TGCA'T
Cuts at: 3417 3417
Size: 8029
NspI r_CATG'y
Cuts at: 1132 1229 3419 5387 6932 1132
Size: 97 2190 1968 1545 2229
Fragments arranged by size:
2229 2190 1968 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8029
PciI A'CATG_T
Cuts at: 6928 6928
Size: 8029
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2207 2561 3795 1108
Size: 1099 354 1234 5342
Fragments arranged by size:
5342 1234 1099 354
PfoI T'CCnGG_A
Cuts at: 3390 4936 5064 3390
Size: 1546 128 6355
Fragments arranged by size:
6355 1546 128
PmeI GTTT'AAAC
Cuts at: 5333 5333
Size: 8029
PmlI CAC'GTG
Cuts at: 2428 3025 2428
Size: 597 7432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2486 2518 6098 6130 7611 7643 2486
Size: 32 3580 32 1481 32 2872
Fragments arranged by size:
3580 2872 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4717 5062 4717
Size: 345 7684
PshAI GACnn'nnGTC
Cuts at: 1613 2417 1613
Size: 804 7225
PsiI TTA'TAA
Cuts at: 5599 5599
Size: 8029
PspOMI G'GGCC_C
Cuts at: 4408 4663 4408
Size: 255 7774
PspXI vC'TCGA_Gb
Cuts at: 4101 4101
Size: 8029
PstI C_TGCA'G
Cuts at: 830 2448 2668 3243 3657 3984 4755 5083 5381
Size: 1618 220 575 414 327 771 328 298
Cuts at: 5381 830
Size: 3478
Fragments arranged by size:
3478 1618 771 575 414 328 327 298
220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8029
PvuII CAG'CTG
Cuts at: 4015 4015
Size: 8029
SalI G'TCGA_C
Cuts at: 1936 5277 5371 1936
Size: 3341 94 4594
Fragments arranged by size:
4594 3341 94
SapI GCTCTTCn'nnn_
Cuts at: 2808 3856 4245 2808
Size: 1048 389 6592
Fragments arranged by size:
6592 1048 389
SbfI CC_TGCA'GG
Cuts at: 5381 5381
Size: 8029
ScaI AGT'ACT
Cuts at: 3323 4486 6277 7989 3323
Size: 1163 1791 1712 3363
Fragments arranged by size:
3363 1791 1712 1163
SfcI C'TryA_G
Cuts at: 826 1045 2444 2664 3239 3653 3902 3980 4751
Size: 219 1399 220 575 414 249 78 771
Cuts at: 4751 5079 5377 6512 7193 7384 826
Size: 328 298 1135 681 191 1471
Fragments arranged by size:
1471 1399 1135 771 681 575 414 328
298 249 220 219 191 78
SfoI GGC'GCC
Cuts at: 2659 3674 2659
Size: 1015 7014
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8029
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8029
SmaI CCC'GGG
Cuts at: 1872 3059 3089 5356 1872
Size: 1187 30 2267 4545
Fragments arranged by size:
4545 2267 1187 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4101 4730 5057 6133 7034 7296
Size: 197 926 2158 629 327 1076 901 262
Cuts at: 7296 820
Size: 1553
Fragments arranged by size:
2158 1553 1076 926 901 629 327 262
197
SnaBI TAC'GTA
Cuts at: 493 5820 493
Size: 5327 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8029
SphI G_CATG'C
Cuts at: 1132 3419 5387 1132
Size: 2287 1968 3774
Fragments arranged by size:
3774 2287 1968
SspI AAT'ATT
Cuts at: 5 52 3186 3830 5953 5
Size: 47 3134 644 2123 2081
Fragments arranged by size:
3134 2123 2081 644 47
StuI AGG'CCT
Cuts at: 1517 2468 3047 1517
Size: 951 579 6499
Fragments arranged by size:
6499 951 579
StyI C'CwwG_G
Cuts at: 513 1065 2004 2233 2683 3072 3126 3502 4705
Size: 552 939 229 450 389 54 376 1203
Cuts at: 4705 4977 5468 513
Size: 272 491 3074
Fragments arranged by size:
3074 1203 939 552 491 450 389 376
272 229 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3328 3413 3976 4272 5037 6056 6073
Size: 1255 1748 85 563 296 765 1019 17
Cuts at: 6073 6226 6411 6856 325
Size: 153 185 445 1498
Fragments arranged by size:
1748 1498 1255 1019 765 563 445 296
185 153 85 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 3321 4377
Size: 274 80 33 51 1443 312 1032 1056
Cuts at: 4377 4484 4968 6275 7987 96
Size: 107 484 1307 1712 138
Fragments arranged by size:
1712 1443 1307 1056 1032 484 312 274
138 107 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2346 2378 2981 3013 3386 3418 7989 8021 2346
Size: 32 603 32 373 32 4571 32 2354
Fragments arranged by size:
4571 2354 603 373 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4310 1316
Size: 2994 5035
XbaI T'CTAG_A
Cuts at: 5365 5365
Size: 8029
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2458 2581 4709 2458
Size: 123 2128 5778
Fragments arranged by size:
5778 2128 123
XhoI C'TCGA_G
Cuts at: 1943 4101 1943
Size: 2158 5871
XmaI C'CCGG_G
Cuts at: 1870 3057 3087 5354 1870
Size: 1187 30 2267 4545
Fragments arranged by size:
4545 2267 1187 30
XmnI GAAnn'nnTTC
Cuts at: 3288 6158 3288
Size: 2870 5159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5837 276
Size: 53 83 186 936 4303 2468
Fragments arranged by size:
4303 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI NruI PacI
PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI