(Circular) (Six-base) MAPSORT of: pFN21AB9001.seq Check: 6668 from: 1 to: 5983 pFN21AB9001 5983 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 1937 Size: 1389 4594 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3123 4046 5430 5698 1632 Size: 1491 923 1384 268 1917 Fragments arranged by size: 1917 1491 1384 923 268 AfeI AGC'GCT Cuts at: 2078 2078 Size: 5983 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 2567 4882 5859 1203 Size: 1364 2315 977 1327 Fragments arranged by size: 2315 1364 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 2896 4712 2896 Size: 1816 4167 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5983 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2643 2675 2722 2754 2643 Size: 32 47 32 5872 Fragments arranged by size: 5872 47 32 32 AlwNI CAG_nnn'CTG Cuts at: 3223 3726 5298 3223 Size: 503 1572 3908 Fragments arranged by size: 3908 1572 503 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 2744 3088 3292 3518 3670 5779 1493 Size: 1251 344 204 226 152 2109 1697 Fragments arranged by size: 2109 1697 1251 344 226 204 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 3308 1870 Size: 73 1365 4545 Fragments arranged by size: 4545 1365 73 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2998 3304 4759 618 Size: 325 217 627 1211 306 1455 1842 Fragments arranged by size: 1842 1455 1211 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2113 2584 3302 1391 Size: 722 471 718 4072 Fragments arranged by size: 4072 722 718 471 BbeI G_GCGC'C Cuts at: 3002 3002 Size: 5983 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2269 928 Size: 971 370 4642 Fragments arranged by size: 4642 971 370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BfrBI ATG'CAT Cuts at: 2643 2643 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4594 136 Size: 107 122 71 631 605 2922 1525 Fragments arranged by size: 2922 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 2174 3400 2174 Size: 1226 4757 Bme1580I G_kGCm'C Cuts at: 1790 4044 5200 1790 Size: 2254 1156 2573 Fragments arranged by size: 2573 2254 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3714 4672 442 Size: 416 53 83 399 24 2297 958 1753 Fragments arranged by size: 2297 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3189 3189 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 4643 1239 Size: 1079 2325 2579 Fragments arranged by size: 2579 2325 1079 Bpu10I CC'TnA_GC Cuts at: 2112 2112 Size: 5983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2057 4108 4973 5271 2057 Size: 2051 865 298 2769 Fragments arranged by size: 2769 2051 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4646 882 Size: 617 3147 2219 Fragments arranged by size: 3147 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3774 493 Size: 711 2570 2702 Fragments arranged by size: 2702 2570 711 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2946 2999 3790 4172 Size: 53 83 186 936 1413 53 791 382 Cuts at: 4172 4807 275 Size: 635 1451 Fragments arranged by size: 1451 1413 936 791 635 382 186 83 53 53 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2643 2673 3185 3215 1178 Size: 30 165 30 1240 30 512 30 3946 Fragments arranged by size: 3946 1240 512 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2315 3190 1186 Size: 1129 875 3979 Fragments arranged by size: 3979 1129 875 BseYI C'CCAG_C Cuts at: 2845 2920 2983 5186 5682 2845 Size: 75 63 2203 496 3146 Fragments arranged by size: 3146 2203 496 75 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3165 1163 Size: 573 1429 3981 Fragments arranged by size: 3981 1429 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2862 4044 4129 5200 2862 Size: 1182 85 1071 3645 Fragments arranged by size: 3645 1182 1071 85 BsmI GAATG_Cn' Cuts at: 3494 3587 3494 Size: 93 5890 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2584 2862 3302 4044 4129 5200 Size: 399 323 471 278 440 742 85 1071 Cuts at: 5200 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 471 440 399 323 278 85 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 2232 2544 2748 3872 5602 2232 Size: 312 204 1124 1730 2613 Fragments arranged by size: 2613 1730 1124 312 204 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 3338 844 Size: 1559 935 3489 Fragments arranged by size: 3489 1559 935 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4478 4652 65 Size: 1183 3230 174 1396 Fragments arranged by size: 3230 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4627 4814 1700 Size: 110 2817 187 2869 Fragments arranged by size: 2869 2817 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5983 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2438 2438 Size: 5983 BstYI r'GATC_y Cuts at: 1972 3313 3680 4065 4082 5534 5620 5632 5978 Size: 1341 367 385 17 1452 86 12 346 Cuts at: 5978 1972 Size: 1977 Fragments arranged by size: 1977 1452 1341 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2586 2586 Size: 5983 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5431 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5244 Fragments arranged by size: 5244 683 56 BtsI GCAGTG_nn' Cuts at: 3156 3166 3499 4293 4313 3156 Size: 10 333 794 20 4826 Fragments arranged by size: 4826 794 333 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 2460 3287 2460 Size: 827 5156 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2520 3430 Size: 54 1425 50 275 18 330 360 910 Cuts at: 3430 4319 5948 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 910 889 360 330 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2831 2846 3913 1353 Size: 1478 15 1067 3423 Fragments arranged by size: 3423 1478 1067 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4570 4944 5090 387 Size: 1064 138 2981 374 146 1280 Fragments arranged by size: 2981 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2318 3123 4046 4643 5430 5698 1239 Size: 393 686 805 923 597 787 268 1524 Fragments arranged by size: 1524 923 805 787 686 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3263 3263 Size: 5983 EcoRI G'AATT_C Cuts at: 2744 3088 3292 3670 2744 Size: 344 204 378 5057 Fragments arranged by size: 5057 378 344 204 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 2080 3002 5130 1678 Size: 402 922 2128 2531 Fragments arranged by size: 2531 2128 922 402 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 2615 2647 2882 Size: 32 135 32 800 32 211 32 235 Cuts at: 2882 2914 3185 3217 4624 4656 4698 4730 1373 Size: 32 271 32 1407 32 42 32 2626 Fragments arranged by size: 2626 1407 800 271 235 211 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2023 3327 3573 669 Size: 988 281 85 1304 246 3079 Fragments arranged by size: 3079 1304 988 281 246 85 HindIII A'AGCT_T Cuts at: 748 2539 748 Size: 1791 4192 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KasI G'GCGC_C Cuts at: 2998 2998 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 3048 3582 3048 Size: 534 5449 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3009 5097 5281 1305 Size: 1704 2088 184 2007 Fragments arranged by size: 2088 2007 1704 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 2522 10 Size: 54 1425 343 330 360 3471 Fragments arranged by size: 3471 1425 360 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 3941 4300 4459 518 Size: 648 2775 359 159 2042 Fragments arranged by size: 2775 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2849 3399 4076 5224 5469 1606 Size: 1243 550 677 1148 245 2120 Fragments arranged by size: 2120 1243 1148 677 550 245 NarI GG'CG_CC Cuts at: 2999 2999 Size: 5983 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5431 NdeI CA'TA_TG Cuts at: 387 2557 387 Size: 2170 3813 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NsiI A_TGCA'T Cuts at: 2645 2645 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2204 3341 4886 1132 Size: 97 975 1137 1545 2229 Fragments arranged by size: 2229 1545 1137 975 97 PasI CC'CwG_GG Cuts at: 1348 3268 1348 Size: 1920 4063 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 3223 1108 Size: 2115 3868 PfoI T'CCnGG_A Cuts at: 2505 2680 2505 Size: 175 5808 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2643 2675 4052 4084 5565 5597 2643 Size: 32 1377 32 1481 32 3029 Fragments arranged by size: 3029 1481 1377 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3263 3263 Size: 5983 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PstI C_TGCA'G Cuts at: 830 3335 830 Size: 2505 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 2035 3331 4466 5147 5338 826 Size: 219 990 1296 1135 681 191 1471 Fragments arranged by size: 1471 1296 1135 990 681 219 191 SfoI GGC'GCC Cuts at: 3000 3000 Size: 5983 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 4087 4988 5250 820 Size: 197 926 129 2015 901 262 1553 Fragments arranged by size: 2015 1553 926 901 262 197 129 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5983 StyI C'CwwG_G Cuts at: 513 1065 3422 513 Size: 552 2357 3074 Fragments arranged by size: 3074 2357 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4010 4027 4180 4365 4810 325 Size: 1255 2430 17 153 185 445 1498 Fragments arranged by size: 2430 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4229 5941 96 Size: 274 80 33 51 1443 2252 1712 138 Fragments arranged by size: 2252 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5943 5975 5943 Size: 32 5951 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2411 2411 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 2658 4112 2658 Size: 1454 4529 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI