(Circular) (Six-base) MAPSORT of: pFN21AB8997.seq Check: 5714 from: 1 to: 5929 pFN21AB8997 5929 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2485 2485 Size: 5929 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3739 278 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3272 1937 Size: 1335 4594 Acc65I G'GTAC_C Cuts at: 3250 3250 Size: 5929 AclI AA'CG_TT Cuts at: 1563 4056 4429 1563 Size: 2493 373 3063 Fragments arranged by size: 3063 2493 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 2938 3992 5376 5644 1632 Size: 623 683 1054 1384 268 1917 Fragments arranged by size: 1917 1384 1054 683 623 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5732 AflIII A'CryG_T Cuts at: 1203 4828 5805 1203 Size: 3625 977 1327 Fragments arranged by size: 3625 1327 977 AgeI A'CCGG_T Cuts at: 4760 4760 Size: 5929 AhdI GACnn_n'nnGTC Cuts at: 4658 4658 Size: 5929 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5929 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5929 AlwNI CAG_nnn'CTG Cuts at: 3082 3672 5244 3082 Size: 590 1572 3767 Fragments arranged by size: 3767 1572 590 ApaI G_GGCC'C Cuts at: 2510 2803 2510 Size: 293 5636 ApaLI G'TGCA_C Cuts at: 3986 5142 3986 Size: 1156 4773 ApoI r'AATT_y Cuts at: 1493 3238 3464 3616 5725 1493 Size: 1745 226 152 2109 1697 Fragments arranged by size: 2109 1745 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4483 160 Size: 872 3451 1606 Fragments arranged by size: 3451 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 AvaI C'yCGr_G Cuts at: 1870 1943 2679 3254 1870 Size: 73 736 575 4545 Fragments arranged by size: 4545 736 575 73 BamHI G'GATC_C Cuts at: 3259 3626 3259 Size: 367 5562 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2575 2583 2931 3222 3250 Size: 325 217 627 788 8 348 291 28 Cuts at: 3250 4705 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 788 627 348 325 291 217 28 8 BanII G_rGCy'C Cuts at: 1391 2163 2510 2660 2803 3248 1391 Size: 772 347 150 143 445 4072 Fragments arranged by size: 4072 772 445 347 150 143 BbeI G_GCGC'C Cuts at: 2935 2935 Size: 5929 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4958 BbvCI CC'TCA_GC Cuts at: 2435 2435 Size: 5929 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2170 2204 4120 4154 1903 Size: 34 233 34 1916 34 3678 Fragments arranged by size: 3678 1916 233 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2331 3116 3821 5031 2331 Size: 785 705 1210 3229 Fragments arranged by size: 3229 1210 785 705 BclI T'GATC_A Cuts at: 1548 1548 Size: 5929 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2342 2838 4540 Size: 107 122 71 631 605 670 496 1702 Cuts at: 4540 136 Size: 1525 Fragments arranged by size: 1702 1525 670 631 605 496 122 107 71 BglII A'GATC_T Cuts at: 5924 5924 Size: 5929 BlpI GC'TnA_GC Cuts at: 3346 3346 Size: 5929 Bme1580I G_kGCm'C Cuts at: 1790 2510 2586 2803 3129 3990 5146 1790 Size: 720 76 217 326 861 1156 2573 Fragments arranged by size: 2573 1156 861 720 326 217 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5929 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 4618 442 Size: 416 53 83 399 24 2243 958 1753 Fragments arranged by size: 2243 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2708 1056 Size: 1652 4277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2479 2479 Size: 5929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 4589 1239 Size: 1163 2187 2579 Fragments arranged by size: 2579 2187 1163 Bpu10I CC'TnA_GC Cuts at: 2143 2325 2435 2886 2143 Size: 182 110 451 5186 Fragments arranged by size: 5186 451 182 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 2766 4054 4919 5217 2336 Size: 430 1288 865 298 3048 Fragments arranged by size: 3048 1288 865 430 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4592 882 Size: 617 3093 2219 Fragments arranged by size: 3093 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3720 493 Size: 711 2516 2702 Fragments arranged by size: 2702 2516 711 BsaBI GATnn'nnATC Cuts at: 5923 5923 Size: 5929 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2137 2863 2932 3736 Size: 53 83 186 936 604 726 69 804 Cuts at: 3736 4118 4753 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 804 726 635 604 382 186 83 69 53 BsaWI w'CCGG_w Cuts at: 2242 4362 4760 5034 5181 5592 2242 Size: 2120 398 274 147 411 2579 Fragments arranged by size: 2579 2120 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5704 Fragments arranged by size: 5704 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2300 2371 2455 1186 Size: 1111 3 71 84 4660 Fragments arranged by size: 4660 1111 84 71 3 BseYI C'CCAG_C Cuts at: 2563 2802 5132 5628 2563 Size: 239 2330 496 2864 Fragments arranged by size: 2864 2330 496 239 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3095 1163 Size: 573 1359 3997 Fragments arranged by size: 3997 1359 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2774 3379 4140 5168 1540 Size: 275 186 773 605 761 1028 2301 Fragments arranged by size: 2301 1028 773 761 605 275 186 BsiHKAI G_wGCw'C Cuts at: 2163 3990 4075 5146 2163 Size: 1827 85 1071 2946 Fragments arranged by size: 2946 1827 1071 85 BsmI GAATG_Cn' Cuts at: 2028 3440 3533 2028 Size: 1412 93 4424 Fragments arranged by size: 4424 1412 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2084 2084 Size: 5929 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2510 2586 2660 2803 3129 3248 Size: 399 373 347 76 74 143 326 119 Cuts at: 3248 3990 4075 5146 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 373 347 326 143 119 85 76 74 BspEI T'CCGG_A Cuts at: 2242 5592 2242 Size: 3350 2579 BspHI T'CATG_A Cuts at: 3818 5548 3818 Size: 1730 4199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2485 3284 844 Size: 1641 799 3489 Fragments arranged by size: 3489 1641 799 BsrBI CCG'CTC Cuts at: 1394 1823 2142 3816 1394 Size: 429 319 1674 3507 Fragments arranged by size: 3507 1674 429 319 BsrDI GCAATG_nn' Cuts at: 65 1248 4424 4598 65 Size: 1183 3176 174 1396 Fragments arranged by size: 3176 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2265 2928 4573 4760 1700 Size: 110 455 663 1645 187 2869 Fragments arranged by size: 2869 1645 663 455 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5929 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5929 BssSI C'ACGA_G Cuts at: 2608 3989 5001 2608 Size: 1381 1012 3536 Fragments arranged by size: 3536 1381 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5929 BstBI TT'CG_AA Cuts at: 4733 4733 Size: 5929 BstEII G'GTnAC_C Cuts at: 1184 2015 2821 1184 Size: 831 806 4292 Fragments arranged by size: 4292 831 806 BstXI CCAn_nnnn'nTGG Cuts at: 2309 2309 Size: 5929 BstYI r'GATC_y Cuts at: 1972 2257 2298 3259 3626 4011 4028 5480 5566 Size: 285 41 961 367 385 17 1452 86 Cuts at: 5566 5578 5924 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 961 385 367 346 285 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 3016 513 Size: 552 1951 3426 Fragments arranged by size: 3426 1951 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2973 487 Size: 683 56 1747 3443 Fragments arranged by size: 3443 1747 683 56 BtsI GCAGTG_nn' Cuts at: 3445 4239 4259 3445 Size: 794 20 5115 Fragments arranged by size: 5115 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5929 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5894 DraI TTT'AAA Cuts at: 3233 3233 Size: 5929 DraIII CAC_nnn'GTG Cuts at: 2629 2629 Size: 5929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4936 809 Size: 507 3620 1802 Fragments arranged by size: 3620 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 2830 2839 Size: 54 1425 50 275 18 504 496 9 Cuts at: 2839 3376 4265 5894 8 Size: 537 889 1629 43 Fragments arranged by size: 1629 1425 889 537 504 496 275 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3376 1537 Size: 275 1564 4090 Fragments arranged by size: 4090 1564 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 2489 3859 1353 Size: 933 203 1370 3423 Fragments arranged by size: 3423 1370 933 203 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4516 4890 5036 387 Size: 1064 138 2927 374 146 1280 Fragments arranged by size: 2927 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2161 2161 Size: 5929 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2402 2938 3992 4589 5376 5644 Size: 393 623 147 536 1054 597 787 268 Cuts at: 5644 1239 Size: 1524 Fragments arranged by size: 1524 1054 787 623 597 536 393 268 147 EcoO109I rG'GnC_Cy Cuts at: 2506 2507 2506 Size: 1 5928 EcoRI G'AATT_C Cuts at: 3238 3616 3238 Size: 378 5551 FspI TGC'GCA Cuts at: 4435 4435 Size: 5929 HaeII r_GCGC'y Cuts at: 1678 2332 2935 5076 1678 Size: 654 603 2141 2531 Fragments arranged by size: 2531 2141 654 603 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4570 4602 4644 4676 1373 Size: 32 135 32 2998 32 42 32 2626 Fragments arranged by size: 2998 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3273 3519 669 Size: 988 281 1335 246 3079 Fragments arranged by size: 3079 1335 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5929 HpaI GTT'AAC Cuts at: 3519 3519 Size: 5929 KasI G'GCGC_C Cuts at: 2931 2931 Size: 5929 KpnI G_GTAC'C Cuts at: 3254 3254 Size: 5929 MfeI C'AATT_G Cuts at: 3528 3528 Size: 5929 MluI A'CGCG_T Cuts at: 5805 5805 Size: 5929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5043 5227 1305 Size: 3738 184 2007 Fragments arranged by size: 3738 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2832 10 Size: 54 1425 343 1000 3107 Fragments arranged by size: 3107 1425 1000 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2625 2714 3149 3887 4246 4405 518 Size: 648 1459 89 435 738 359 159 2042 Fragments arranged by size: 2042 1459 738 648 435 359 159 89 MspA1I CmG'CkG Cuts at: 1606 2902 3345 4022 5170 5415 1606 Size: 1296 443 677 1148 245 2120 Fragments arranged by size: 2120 1296 1148 677 443 245 NaeI GCC'GGC Cuts at: 2930 2930 Size: 5929 NarI GG'CG_CC Cuts at: 2932 2932 Size: 5929 NcoI C'CATG_G Cuts at: 513 1065 3016 513 Size: 552 1951 3426 Fragments arranged by size: 3426 1951 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5929 NgoMIV G'CCGG_C Cuts at: 2928 2928 Size: 5929 NheI G'CTAG_C Cuts at: 1052 2704 1052 Size: 1652 4277 NotI GC'GGCC_GC Cuts at: 3376 3376 Size: 5929 NspI r_CATG'y Cuts at: 1132 1229 2254 2759 3287 4832 1132 Size: 97 1025 505 528 1545 2229 Fragments arranged by size: 2229 1545 1025 528 505 97 PasI CC'CwG_GG Cuts at: 1348 2393 1348 Size: 1045 4884 PciI A'CATG_T Cuts at: 4828 4828 Size: 5929 PflMI CCAn_nnn'nTGG Cuts at: 1108 2699 1108 Size: 1591 4338 PfoI T'CCnGG_A Cuts at: 2487 2487 Size: 5929 PmeI GTTT'AAAC Cuts at: 3233 3233 Size: 5929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3998 4030 5511 5543 3998 Size: 32 1481 32 4384 Fragments arranged by size: 4384 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3083 1613 Size: 1470 4459 PsiI TTA'TAA Cuts at: 3499 3499 Size: 5929 PspOMI G'GGCC_C Cuts at: 2506 2799 2506 Size: 293 5636 PstI C_TGCA'G Cuts at: 830 2193 2473 3281 830 Size: 1363 280 808 3478 Fragments arranged by size: 3478 1363 808 280 PvuI CG_AT'CG Cuts at: 2001 2774 2001 Size: 773 5156 SacI G_AGCT'C Cuts at: 2163 2163 Size: 5929 SalI G'TCGA_C Cuts at: 1936 3271 1936 Size: 1335 4594 SapI GCTCTTCn'nnn_ Cuts at: 2286 2489 2286 Size: 203 5726 SbfI CC_TGCA'GG Cuts at: 3281 3281 Size: 5929 ScaI AGT'ACT Cuts at: 4177 5889 4177 Size: 1712 4217 SfcI C'TryA_G Cuts at: 826 1045 2189 2469 3277 4412 5093 5284 826 Size: 219 1144 280 808 1135 681 191 1471 Fragments arranged by size: 1471 1144 1135 808 681 280 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2342 2838 2342 Size: 496 5433 SfoI GGC'GCC Cuts at: 2933 2933 Size: 5929 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5929 SmaI CCC'GGG Cuts at: 1872 2681 3256 1872 Size: 809 575 4545 Fragments arranged by size: 4545 809 575 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 2781 4033 4934 5196 820 Size: 197 926 372 466 1252 901 262 1553 Fragments arranged by size: 1553 1252 926 901 466 372 262 197 SnaBI TAC'GTA Cuts at: 493 3720 493 Size: 3227 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5929 SphI G_CATG'C Cuts at: 1132 3287 1132 Size: 2155 3774 SspI AAT'ATT Cuts at: 5 52 3853 5 Size: 47 3801 2081 Fragments arranged by size: 3801 2081 47 StuI AGG'CCT Cuts at: 1517 3005 1517 Size: 1488 4441 StyI C'CwwG_G Cuts at: 513 1065 3016 3368 513 Size: 552 1951 352 3074 Fragments arranged by size: 3074 1951 552 352 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3210 3956 3973 4126 4311 4756 325 Size: 1255 1630 746 17 153 185 445 1498 Fragments arranged by size: 1630 1498 1255 746 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4175 5887 96 Size: 274 80 33 51 1443 2198 1712 138 Fragments arranged by size: 2198 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5889 5921 5889 Size: 32 5897 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5929 XbaI T'CTAG_A Cuts at: 3265 3265 Size: 5929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3157 3157 Size: 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5929 XmaI C'CCGG_G Cuts at: 1870 2679 3254 1870 Size: 809 575 4545 Fragments arranged by size: 4545 809 575 XmnI GAAnn'nnTTC Cuts at: 4058 4058 Size: 5929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3737 276 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SrfI SwaI