Restriction map for
pFN21AB8997
(Linear) (Six-base) MAPSORT of: KIBB8997.seq Check: 2780 from: 1 to: 1224
KIBB8997.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 480 1224
Size: 480 744
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 933 1224
Size: 250 683 291
Fragments arranged by size:
683 291 250
AlwNI CAG_nnn'CTG
Cuts at: 0 1077 1224
Size: 1077 147
ApaI G_GGCC'C
Cuts at: 0 505 798 1224
Size: 505 293 426
Fragments arranged by size:
505 426 293
AvaI C'yCGr_G
Cuts at: 0 674 1224
Size: 674 550
BanI G'GyrC_C
Cuts at: 0 570 578 926 1217 1224
Size: 570 8 348 291 7
Fragments arranged by size:
570 348 291 8 7
BanII G_rGCy'C
Cuts at: 0 158 505 655 798 1224
Size: 158 347 150 143 426
Fragments arranged by size:
426 347 158 150 143
BbeI G_GCGC'C
Cuts at: 0 930 1224
Size: 930 294
BbvCI CC'TCA_GC
Cuts at: 0 430 1224
Size: 430 794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 165 199 1224
Size: 165 34 1025
Fragments arranged by size:
1025 165 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 326 1111 1224
Size: 326 785 113
Fragments arranged by size:
785 326 113
BglI GCCn_nnn'nGGC
Cuts at: 0 337 833 1224
Size: 337 496 391
Fragments arranged by size:
496 391 337
Bme1580I G_kGCm'C
Cuts at: 0 505 581 798 1124 1224
Size: 505 76 217 326 100
Fragments arranged by size:
505 326 217 100 76
BmtI G_CTAG'C
Cuts at: 0 703 1224
Size: 703 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 474 1224
Size: 474 750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1224
Size: 397 827
Bpu10I CC'TnA_GC
Cuts at: 0 138 320 430 881 1224
Size: 138 182 110 451 343
Fragments arranged by size:
451 343 182 138 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 761 1224
Size: 331 430 463
Fragments arranged by size:
463 430 331
BsaHI Gr'CG_yC
Cuts at: 0 132 858 927 1224
Size: 132 726 69 297
Fragments arranged by size:
726 297 132 69
BsaWI w'CCGG_w
Cuts at: 0 237 1224
Size: 237 987
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 295 366 450 1224
Size: 292 3 71 84 774
Fragments arranged by size:
774 292 84 71 3
BseYI C'CCAG_C
Cuts at: 0 558 797 1224
Size: 558 239 427
Fragments arranged by size:
558 427 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 1224
Size: 1090 134
BsiEI CG_ry'CG
Cuts at: 0 769 1224
Size: 769 455
BsiHKAI G_wGCw'C
Cuts at: 0 158 1224
Size: 158 1066
BsmI GAATG_Cn'
Cuts at: 0 23 1224
Size: 23 1201
BsmBI CGTCTCn'nnnn_
Cuts at: 0 79 1224
Size: 79 1145
Bsp1286I G_dGCh'C
Cuts at: 0 158 505 581 655 798 1124 1224
Size: 158 347 76 74 143 326 100
Fragments arranged by size:
347 326 158 143 100 76 74
BspEI T'CCGG_A
Cuts at: 0 237 1224
Size: 237 987
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 480 1224
Size: 480 744
BsrBI CCG'CTC
Cuts at: 0 137 1224
Size: 137 1087
BsrFI r'CCGG_y
Cuts at: 0 260 923 1224
Size: 260 663 301
Fragments arranged by size:
663 301 260
BssSI C'ACGA_G
Cuts at: 0 603 1224
Size: 603 621
BstEII G'GTnAC_C
Cuts at: 0 10 816 1224
Size: 10 806 408
Fragments arranged by size:
806 408 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 1224
Size: 304 920
BstYI r'GATC_y
Cuts at: 0 252 293 1224
Size: 252 41 931
Fragments arranged by size:
931 252 41
BtgI C'CryG_G
Cuts at: 0 1011 1224
Size: 1011 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 968 1224
Size: 968 256
DraIII CAC_nnn'GTG
Cuts at: 0 624 1224
Size: 624 600
EaeI y'GGCC_r
Cuts at: 0 329 825 834 1224
Size: 329 496 9 390
Fragments arranged by size:
496 390 329 9
EarI CTCTTCn'nnn_
Cuts at: 0 281 484 1224
Size: 281 203 740
Fragments arranged by size:
740 281 203
EcoICRI GAG'CTC
Cuts at: 0 156 1224
Size: 156 1068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 397 933 1224
Size: 250 147 536 291
Fragments arranged by size:
536 291 250 147
EcoO109I rG'GnC_Cy
Cuts at: 0 501 502 1224
Size: 501 1 722
Fragments arranged by size:
722 501 1
HaeII r_GCGC'y
Cuts at: 0 327 930 1224
Size: 327 603 294
Fragments arranged by size:
603 327 294
KasI G'GCGC_C
Cuts at: 0 926 1224
Size: 926 298
MscI TGG'CCA
Cuts at: 0 827 1224
Size: 827 397
MslI CAynn'nnrTG
Cuts at: 0 620 709 1144 1224
Size: 620 89 435 80
Fragments arranged by size:
620 435 89 80
MspA1I CmG'CkG
Cuts at: 0 897 1224
Size: 897 327
NaeI GCC'GGC
Cuts at: 0 925 1224
Size: 925 299
NarI GG'CG_CC
Cuts at: 0 927 1224
Size: 927 297
NcoI C'CATG_G
Cuts at: 0 1011 1224
Size: 1011 213
NgoMIV G'CCGG_C
Cuts at: 0 923 1224
Size: 923 301
NheI G'CTAG_C
Cuts at: 0 699 1224
Size: 699 525
NspI r_CATG'y
Cuts at: 0 249 754 1224
Size: 249 505 470
Fragments arranged by size:
505 470 249
PasI CC'CwG_GG
Cuts at: 0 388 1224
Size: 388 836
PflMI CCAn_nnn'nTGG
Cuts at: 0 694 1224
Size: 694 530
PfoI T'CCnGG_A
Cuts at: 0 482 1224
Size: 482 742
PshAI GACnn'nnGTC
Cuts at: 0 1078 1224
Size: 1078 146
PspOMI G'GGCC_C
Cuts at: 0 501 794 1224
Size: 501 293 430
Fragments arranged by size:
501 430 293
PstI C_TGCA'G
Cuts at: 0 188 468 1224
Size: 188 280 756
Fragments arranged by size:
756 280 188
PvuI CG_AT'CG
Cuts at: 0 769 1224
Size: 769 455
SacI G_AGCT'C
Cuts at: 0 158 1224
Size: 158 1066
SapI GCTCTTCn'nnn_
Cuts at: 0 281 484 1224
Size: 281 203 740
Fragments arranged by size:
740 281 203
SfcI C'TryA_G
Cuts at: 0 184 464 1224
Size: 184 280 760
Fragments arranged by size:
760 280 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 337 833 1224
Size: 337 496 391
Fragments arranged by size:
496 391 337
SfoI GGC'GCC
Cuts at: 0 928 1224
Size: 928 296
SmaI CCC'GGG
Cuts at: 0 676 1224
Size: 676 548
SmlI C'TyrA_G
Cuts at: 0 310 776 1224
Size: 310 466 448
Fragments arranged by size:
466 448 310
StuI AGG'CCT
Cuts at: 0 1000 1224
Size: 1000 224
StyI C'CwwG_G
Cuts at: 0 1011 1224
Size: 1011 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1205 1224
Size: 1205 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1152 1224
Size: 1152 72
XmaI C'CCGG_G
Cuts at: 0 674 1224
Size: 674 550
Enzymes that do cut:
AarI AcuI AlwNI ApaI AvaI BanI BanII BbeI
BbvCI BcgI BciVI BglI Bme1580I BmtI BplI BpmI
Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssSI BstEII BstXI BstYI BtgI BtgZI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I HaeII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PflMI PfoI PshAI PspOMI PstI PvuI SacI SapI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BclI BfrBI BglII
BlpI BmgBI BmrI BsaI BsaAI BsaBI BsaXI BsiWI
BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NdeI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI
XmnI ZraI