Restriction map for
pFN21AB8990
(Linear) (Six-base) MAPSORT of: KIBB8990.seq Check: 939 from: 1 to: 1173
KIBB8990.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 735 1173
Size: 735 438
AfeI AGC'GCT
Cuts at: 0 1012 1173
Size: 1012 161
AlwNI CAG_nnn'CTG
Cuts at: 0 434 750 1173
Size: 434 316 423
Fragments arranged by size:
434 423 316
ApaI G_GGCC'C
Cuts at: 0 107 1173
Size: 107 1066
BanII G_rGCy'C
Cuts at: 0 107 568 1173
Size: 107 461 605
Fragments arranged by size:
605 461 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 234 1173
Size: 234 939
BbvCI CC'TCA_GC
Cuts at: 0 235 1173
Size: 235 938
BciVI GTATCCnnnnn_n'
Cuts at: 0 870 1173
Size: 870 303
BglII A'GATC_T
Cuts at: 0 278 1173
Size: 278 895
Bme1580I G_kGCm'C
Cuts at: 0 56 107 1105 1173
Size: 56 51 998 68
Fragments arranged by size:
998 68 56 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 1164 1173
Size: 1164 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 1173
Size: 888 285
Bpu10I CC'TnA_GC
Cuts at: 0 235 562 1173
Size: 235 327 611
Fragments arranged by size:
611 327 235
BsaAI yAC'GTr
Cuts at: 0 996 1173
Size: 996 177
BsaBI GATnn'nnATC
Cuts at: 0 736 1173
Size: 736 437
BsaWI w'CCGG_w
Cuts at: 0 584 1173
Size: 584 589
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 810 1173
Size: 810 363
BseYI C'CCAG_C
Cuts at: 0 156 515 1173
Size: 156 359 658
Fragments arranged by size:
658 359 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 249 711 1173
Size: 235 14 462 462
Fragments arranged by size:
462 462 235 14
BsiEI CG_ry'CG
Cuts at: 0 462 1173
Size: 462 711
BsiHKAI G_wGCw'C
Cuts at: 0 568 1173
Size: 568 605
BsmI GAATG_Cn'
Cuts at: 0 405 1173
Size: 405 768
BsmBI CGTCTCn'nnnn_
Cuts at: 0 373 1173
Size: 373 800
Bsp1286I G_dGCh'C
Cuts at: 0 56 107 568 1105 1173
Size: 56 51 461 537 68
Fragments arranged by size:
537 461 68 56 51
BspEI T'CCGG_A
Cuts at: 0 584 1173
Size: 584 589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 735 1058 1173
Size: 735 323 115
Fragments arranged by size:
735 323 115
BsrGI T'GTAC_A
Cuts at: 0 422 1173
Size: 422 751
BstAPI GCAn_nnn'nTGC
Cuts at: 0 741 750 974 1173
Size: 741 9 224 199
Fragments arranged by size:
741 224 199 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1086 1125 1173
Size: 492 594 39 48
Fragments arranged by size:
594 492 48 39
BstYI r'GATC_y
Cuts at: 0 278 782 1173
Size: 278 504 391
Fragments arranged by size:
504 391 278
BtgI C'CryG_G
Cuts at: 0 59 491 803 1097 1124 1173
Size: 59 432 312 294 27 49
Fragments arranged by size:
432 312 294 59 49 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 620 871 1173
Size: 620 251 302
Fragments arranged by size:
620 302 251
BtsI GCAGTG_nn'
Cuts at: 0 1105 1173
Size: 1105 68
ClaI AT'CG_AT
Cuts at: 0 929 1173
Size: 929 244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 133 168 1173
Size: 133 35 1005
Fragments arranged by size:
1005 133 35
EaeI y'GGCC_r
Cuts at: 0 209 296 1173
Size: 209 87 877
Fragments arranged by size:
877 209 87
EcoICRI GAG'CTC
Cuts at: 0 566 1173
Size: 566 607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 1173
Size: 888 285
EcoO109I rG'GnC_Cy
Cuts at: 0 103 119 1173
Size: 103 16 1054
Fragments arranged by size:
1054 103 16
EcoRV GAT'ATC
Cuts at: 0 20 1173
Size: 20 1153
HaeII r_GCGC'y
Cuts at: 0 77 1014 1173
Size: 77 937 159
Fragments arranged by size:
937 159 77
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1173
Size: 377 796
MscI TGG'CCA
Cuts at: 0 211 1173
Size: 211 962
MslI CAynn'nnrTG
Cuts at: 0 490 577 673 745 1084 1123 1173
Size: 490 87 96 72 339 39 50
Fragments arranged by size:
490 339 96 87 72 50 39
MspA1I CmG'CkG
Cuts at: 0 263 908 974 1173
Size: 263 645 66 199
Fragments arranged by size:
645 263 199 66
NcoI C'CATG_G
Cuts at: 0 491 1097 1173
Size: 491 606 76
Fragments arranged by size:
606 491 76
NspI r_CATG'y
Cuts at: 0 84 678 1173
Size: 84 594 495
Fragments arranged by size:
594 495 84
PfoI T'CCnGG_A
Cuts at: 0 872 1173
Size: 872 301
PpuMI rG'GwC_Cy
Cuts at: 0 119 1173
Size: 119 1054
PspOMI G'GGCC_C
Cuts at: 0 103 1173
Size: 103 1070
PstI C_TGCA'G
Cuts at: 0 1069 1151 1173
Size: 1069 82 22
Fragments arranged by size:
1069 82 22
PvuII CAG'CTG
Cuts at: 0 263 908 974 1173
Size: 263 645 66 199
Fragments arranged by size:
645 263 199 66
SacI G_AGCT'C
Cuts at: 0 568 1173
Size: 568 605
SfcI C'TryA_G
Cuts at: 0 528 963 1065 1147 1173
Size: 528 435 102 82 26
Fragments arranged by size:
528 435 102 82 26
StyI C'CwwG_G
Cuts at: 0 491 1097 1173
Size: 491 606 76
Fragments arranged by size:
606 491 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 585 1173
Size: 183 402 588
Fragments arranged by size:
588 402 183
TatI w'GTAC_w
Cuts at: 0 422 599 1173
Size: 422 177 574
Fragments arranged by size:
574 422 177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 653 1173
Size: 653 520
XmnI GAAnn'nnTTC
Cuts at: 0 1079 1173
Size: 1079 94
Enzymes that do cut:
AarI AfeI AlwNI ApaI BanII BbsI BbvCI BciVI
BglII Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrGI BstAPI BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI EaeI EcoICRI Eco57MI EcoO109I EcoRV
HaeII MmeI MscI MslI MspA1I NcoI NspI PfoI
PpuMI PspOMI PstI PvuII SacI SfcI StyI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI
BsaI BsaHI BsaXI BsiWI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I DraI DraIII
DrdI EagI EarI EciI EcoNI EcoRI FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
ZraI