Restriction map for
pFN21AB8980
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BsgI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI NcoI NdeI NheI NlaIII
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AlwI 424 GGATC GGATC(4/5)
AlwNI 1114 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 940 CCTGAGG CC^T[ACGT]AGG
Aor51HI 531 AGCGCT AGC^GCT
ApaI 722 GGGCCC GGGCC^C
AvaI 277 CCCGAG C^[CT]CG[GA]G
AvaII 954 GGTCC G^G[AT]CC
BbsI 687 GAAGAC GAAGAC(2/6)
BbvCI 378 CCTCAGC CCTCAGC(-5/-2)
BfaI 466 CTAG C^TAG
BglI 227 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 730 CTGGAG CTGGAG(16/14)
BpuAI 687 GAAGAC GAAGAC(2/6)
BsaWI 578 ACCGGA [AT]^CCGG[AT]
BsmBI 714 CGTCTC CGTCTC(1/5)
BsoBI 277 CCCGAG C^[CT]CG[GA]G
BsrDI 836 GCAATG GCAATG(2/0)
Bsu36I 940 CCTGAGG CC^T[ACGT]AGG
DpnII 425 GATC ^GATC
EarI 682 GAAGAG CTCTTC(1/4)
Eco47III 531 AGCGCT AGC^GCT
Eco81I 940 CCTGAGG CC^T[ACGT]AGG
HincII 894 GTCAAC GT[CT]^[GA]AC
HindII 894 GTCAAC GT[CT]^[GA]AC
Ksp632I 682 GAAGAG CTCTTC(1/4)
MaeI 466 CTAG C^TAG
MboI 425 GATC ^GATC
MseI 829 TTAA T^TAA
NdeII 425 GATC ^GATC
NotI 209 GCGGCCGC GC^GGCCGC
PpuMI 953 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1064 CAGCTG CAG^CTG
Sau3AI 425 GATC ^GATC
SgrAI 258 CGCCGGCG C[GA]^CCGG[CT]G
Tru9I 829 TTAA T^TAA
XbaI 465 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 10 GAATTT [GA]^AATT[CT]
481 GAATTT
AcyI 262 GGCGCC G[GA]^CG[CT]C
431 GGCGCC
AfaI 282 GTAC GT^AC
498 GTAC
ApoI 10 GAATTT [GA]^AATT[CT]
481 GAATTT
BanII 225 GAGCCC G[GA]GC[CT]^C
722 GGGCCC
BbeI 262 GGCGCC GGCGC^C
431 GGCGCC
BcnI 181 CCCGG CC^[GC]GG
249 CCCGG
BsaHI 262 GGCGCC G[GA]^CG[CT]C
431 GGCGCC
BseRI 146 CTCCTC GAGGAG(10/8)
670 GAGGAG
BsiEI 210 CGGCCG CG[GA][CT]^CG
332 CGGCCG
BsmFI 955 GTCCC GGGAC(10/14)
1049 GTCCC
BstF5I 290 GGATG GGATG(2/0)
584 GGATG
BstXI 198 CCACGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
906 CCAGAACTGTGG
EagI 210 CGGCCG C^GGCCG
332 CGGCCG
EclXI 210 CGGCCG C^GGCCG
332 CGGCCG
Eco52I 210 CGGCCG C^GGCCG
332 CGGCCG
FokI 290 GGATG GGATG(9/13)
584 GGATG
Hin1I 262 GGCGCC G[GA]^CG[CT]C
431 GGCGCC
KasI 262 GGCGCC G^GCGCC
431 GGCGCC
KspI 230 CCGCGG CCGC^GG
240 CCGCGG
MaeIII 188 GTCAC ^GT[ACGT]AC
870 GTCAC
NaeI 259 GCCGGC GCC^GGC
330 GCCGGC
NarI 262 GGCGCC GG^CGCC
431 GGCGCC
NciI 181 CCCGG CC^[GC]GG
249 CCCGG
NgoMIV 259 GCCGGC G^CCGGC
330 GCCGGC
PleI 655 GACTC GAGTC(4/5)
802 GACTC
PstI 268 CTGCAG CTGCA^G
1000 CTGCAG
RsaI 282 GTAC GT^AC
498 GTAC
SacII 230 CCGCGG CCGC^GG
240 CCGCGG
SfaNI 1012 GATGC GCATC(5/9)
1116 GCATC
SfcI 268 CTGCAG C^T[GA][CT]AG
1000 CTGCAG
SfoI 262 GGCGCC GGC^GCC
431 GGCGCC
SmlI 649 CTTGAG C^T[CT][GA]AG
949 CTTGAG
Tsp45I 188 GTCAC ^GT[GC]AC
870 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrFI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIV Sau96I ScrFI TaqI TfiI
TseI Tsp509I TspRI TthHB8I