(Circular) (Six-base) MAPSORT of: pFN21AB8980.seq Check: 746 from: 1 to: 5833 pFN21AB8980 5833 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3896 5280 5548 1632 Size: 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 268 AfeI AGC'GCT Cuts at: 2538 2538 Size: 5833 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2273 1184 Size: 1089 4744 AlwNI CAG_nnn'CTG Cuts at: 3124 3576 5148 3124 Size: 452 1572 3809 Fragments arranged by size: 3809 1572 452 ApaI G_GGCC'C Cuts at: 2731 2731 Size: 5833 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 2015 2486 3142 3368 3520 5629 1493 Size: 522 471 656 226 152 2109 1697 Fragments arranged by size: 2109 1697 656 522 471 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 2282 3158 1870 Size: 73 339 876 4545 Fragments arranged by size: 4545 876 339 73 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2257 2267 2436 3154 4609 Size: 325 217 627 470 10 169 718 1455 Cuts at: 4609 618 Size: 1842 Fragments arranged by size: 1842 1455 718 627 470 325 217 169 10 BanII G_rGCy'C Cuts at: 1391 2234 2731 3152 1391 Size: 843 497 421 4072 Fragments arranged by size: 4072 843 497 421 BbeI G_GCGC'C Cuts at: 2271 2440 2271 Size: 169 5664 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2699 928 Size: 971 800 4062 Fragments arranged by size: 4062 971 800 BbvCI CC'TCA_GC Cuts at: 2384 2384 Size: 5833 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4024 4058 1903 Size: 34 2087 34 3678 Fragments arranged by size: 3678 2087 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2238 4444 136 Size: 107 122 71 631 605 566 2206 1525 Fragments arranged by size: 2206 1525 631 605 566 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 2262 2731 3894 5050 1790 Size: 472 469 1163 1156 2573 Fragments arranged by size: 2573 1163 1156 472 469 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2605 2816 3024 Size: 416 53 83 399 24 1188 211 208 Cuts at: 3024 3564 4522 442 Size: 540 958 1753 Fragments arranged by size: 1753 1188 958 540 416 399 211 208 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2980 2980 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2756 4493 1239 Size: 1517 1737 2579 Fragments arranged by size: 2579 1737 1517 Bpu10I CC'TnA_GC Cuts at: 2384 2714 2384 Size: 330 5503 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2675 2975 3958 4823 5121 2675 Size: 300 983 865 298 3387 Fragments arranged by size: 3387 983 865 300 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4496 882 Size: 617 2997 2219 Fragments arranged by size: 2997 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2268 2437 3640 4022 Size: 53 83 186 936 735 169 1203 382 Cuts at: 4022 4657 275 Size: 635 1451 Fragments arranged by size: 1451 1203 936 735 635 382 186 169 83 53 BsaWI w'CCGG_w Cuts at: 2583 4266 4664 4938 5085 5496 2583 Size: 1683 398 274 147 411 2920 Fragments arranged by size: 2920 1683 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5608 Fragments arranged by size: 5608 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2142 2690 1186 Size: 956 548 4329 Fragments arranged by size: 4329 956 548 BseYI C'CCAG_C Cuts at: 2174 5036 5532 2174 Size: 2862 496 2475 Fragments arranged by size: 2862 2475 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5260 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2218 2340 3283 4044 5072 1540 Size: 275 186 217 122 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 217 186 122 BsiHKAI G_wGCw'C Cuts at: 3894 3979 5050 3894 Size: 85 1071 4677 Fragments arranged by size: 4677 1071 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 BsmBI CGTCTCn'nnnn_ Cuts at: 2725 2725 Size: 5833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2262 2731 3152 3894 3979 5050 Size: 399 444 28 469 421 742 85 1071 Cuts at: 5050 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 469 444 421 399 85 28 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3188 844 Size: 2344 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2848 4328 4502 65 Size: 1183 1600 1480 174 1396 Fragments arranged by size: 1600 1480 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2264 2335 2369 4477 4664 1700 Size: 110 454 71 34 2108 187 2869 Fragments arranged by size: 2869 2108 454 187 110 71 34 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5833 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstXI CCAn_nnnn'nTGG Cuts at: 2210 2918 2210 Size: 708 5125 BstYI r'GATC_y Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828 Size: 1191 367 385 17 1452 86 12 346 Cuts at: 5828 1972 Size: 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2946 2946 Size: 5833 BtgI C'CryG_G Cuts at: 513 1065 2203 2235 2245 513 Size: 552 1138 32 10 4101 Fragments arranged by size: 4101 1138 552 32 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 2313 2337 Size: 54 1425 50 275 18 385 98 24 Cuts at: 2337 3280 4169 5798 8 Size: 943 889 1629 43 Fragments arranged by size: 1629 1425 943 889 385 275 98 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2215 2337 3280 1537 Size: 275 403 122 943 4090 Fragments arranged by size: 4090 943 403 275 122 EarI CTCTTCn'nnn_ Cuts at: 1353 2682 3763 1353 Size: 1329 1081 3423 Fragments arranged by size: 3423 1329 1081 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2756 3896 4493 5280 5548 1239 Size: 393 1124 1140 597 787 268 1524 Fragments arranged by size: 1524 1140 1124 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2727 2728 2950 2959 2727 Size: 1 222 9 5601 Fragments arranged by size: 5601 222 9 1 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2716 2716 Size: 5833 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 2211 2271 2440 2540 4980 1678 Size: 533 60 169 100 2440 2531 Fragments arranged by size: 2531 2440 533 169 100 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2137 2169 4474 4506 4548 Size: 32 135 32 565 32 2305 32 42 Cuts at: 4548 4580 1373 Size: 32 2626 Fragments arranged by size: 2626 2305 565 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3177 3423 669 Size: 988 281 963 276 246 3079 Fragments arranged by size: 3079 988 963 281 276 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KasI G'GCGC_C Cuts at: 2267 2436 2267 Size: 169 5664 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4947 5131 1305 Size: 3642 184 2007 Fragments arranged by size: 3642 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3791 4150 4309 518 Size: 648 2625 359 159 2042 Fragments arranged by size: 2625 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2237 2247 2421 3071 3249 3926 5074 5319 Size: 631 10 174 650 178 677 1148 245 Cuts at: 5319 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 650 631 245 178 174 10 NaeI GCC'GGC Cuts at: 2266 2337 2266 Size: 71 5762 NarI GG'CG_CC Cuts at: 2268 2437 2268 Size: 169 5664 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5281 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NgoMIV G'CCGG_C Cuts at: 2264 2335 2264 Size: 71 5762 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 2215 3280 2215 Size: 1065 4768 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 1132 Size: 97 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2959 2959 Size: 5833 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PspOMI G'GGCC_C Cuts at: 2727 2727 Size: 5833 PstI C_TGCA'G Cuts at: 830 2277 3009 3185 830 Size: 1447 732 176 3478 Fragments arranged by size: 3478 1447 732 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 3071 3071 Size: 5833 SacII CC_GC'GG Cuts at: 2238 2248 2238 Size: 10 5823 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 2273 3005 3181 4316 4997 5188 826 Size: 219 1228 732 176 1135 681 191 1471 Fragments arranged by size: 1471 1228 1135 732 681 219 191 176 SfoI GGC'GCC Cuts at: 2269 2438 2269 Size: 169 5664 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 2264 1700 Size: 564 5269 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 2954 3937 4838 5100 820 Size: 197 926 711 300 983 901 262 1553 Fragments arranged by size: 1553 983 926 901 711 300 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 3272 513 Size: 552 2207 3074 Fragments arranged by size: 3074 2207 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2893 3043 3860 3877 4030 4215 4660 Size: 1255 1313 150 817 17 153 185 445 Cuts at: 4660 325 Size: 1498 Fragments arranged by size: 1498 1313 1255 817 445 185 153 150 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4079 5791 96 Size: 274 80 33 51 1443 2102 1712 138 Fragments arranged by size: 2102 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2381 2413 5793 5825 2381 Size: 32 3380 32 2389 Fragments arranged by size: 3380 2389 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 2470 3169 2470 Size: 699 5134 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI