(Circular) (Six-base) MAPSORT of: pFN21AB8978.seq Check: 5424 from: 1 to: 5827 pFN21AB8978 5827 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2857 2857 Size: 5827 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3637 278 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 3148 3148 Size: 5827 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2243 3092 3117 3890 5274 5542 1632 Size: 611 849 25 773 1384 268 1917 Fragments arranged by size: 1917 1384 849 773 611 268 25 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 2867 1166 Size: 1701 4126 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 3570 5142 3570 Size: 1572 4255 ApaI G_GGCC'C Cuts at: 2131 2131 Size: 5827 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 2105 2813 3136 3362 3514 5623 1493 Size: 612 708 323 226 152 2109 1697 Fragments arranged by size: 2109 1697 708 612 323 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2671 3152 1870 Size: 73 728 481 4545 Fragments arranged by size: 4545 728 481 73 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3148 4603 618 Size: 325 217 627 1361 1455 1842 Fragments arranged by size: 1842 1455 1361 627 325 217 BanII G_rGCy'C Cuts at: 1391 2131 2302 3094 3146 1391 Size: 740 171 792 52 4072 Fragments arranged by size: 4072 792 740 171 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3098 928 Size: 971 1199 3657 Fragments arranged by size: 3657 1199 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4018 4052 1903 Size: 34 50 34 1997 34 3678 Fragments arranged by size: 3678 1997 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 2490 1548 Size: 942 4885 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4438 136 Size: 107 122 71 631 605 2766 1525 Fragments arranged by size: 2766 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 2131 3888 5044 1790 Size: 341 1757 1156 2573 Fragments arranged by size: 2573 1757 1156 341 BmgBI CAC'GTC Cuts at: 1318 2113 1318 Size: 795 5032 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3558 4516 442 Size: 416 53 83 399 24 2141 958 1753 Fragments arranged by size: 2141 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4487 1239 Size: 3248 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3952 4817 5115 3952 Size: 865 298 4664 Fragments arranged by size: 4664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3010 4490 882 Size: 617 1511 1480 2219 Fragments arranged by size: 2219 1511 1480 617 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3634 4016 4651 275 Size: 53 83 186 936 2101 382 635 1451 Fragments arranged by size: 2101 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4260 4658 4932 5079 5490 4260 Size: 398 274 147 411 4597 Fragments arranged by size: 4597 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3036 3066 1178 Size: 30 165 30 1633 30 3939 Fragments arranged by size: 3939 1633 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3044 1186 Size: 1858 3969 BseYI C'CCAG_C Cuts at: 5030 5526 5030 Size: 496 5331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5254 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 4038 5066 1540 Size: 275 186 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2095 3888 3973 5044 2095 Size: 1793 85 1071 2878 Fragments arranged by size: 2878 1793 1071 85 BsmI GAATG_Cn' Cuts at: 2338 3338 3431 2338 Size: 1000 93 4734 Fragments arranged by size: 4734 1000 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2131 2302 3094 3146 3888 3973 Size: 399 305 36 171 792 52 742 85 Cuts at: 3973 5044 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 792 742 399 305 171 85 52 36 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 2748 3716 5446 2748 Size: 968 1730 3129 Fragments arranged by size: 3129 1730 968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2253 2857 3182 844 Size: 1409 604 325 3489 Fragments arranged by size: 3489 1409 604 325 BsrBI CCG'CTC Cuts at: 1394 1823 2052 2146 3714 1394 Size: 429 229 94 1568 3507 Fragments arranged by size: 3507 1568 429 229 94 BsrDI GCAATG_nn' Cuts at: 65 1248 2343 4322 4496 65 Size: 1183 1095 1979 174 1396 Fragments arranged by size: 1979 1396 1183 1095 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4471 4658 1700 Size: 110 2661 187 2869 Fragments arranged by size: 2869 2661 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 2589 3887 4899 2589 Size: 1298 1012 3517 Fragments arranged by size: 3517 1298 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 2464 1184 Size: 1280 4547 BstXI CCAn_nnnn'nTGG Cuts at: 2595 2851 2595 Size: 256 5571 BstYI r'GATC_y Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822 Size: 1185 367 385 17 1452 86 12 346 Cuts at: 5822 1972 Size: 1977 Fragments arranged by size: 1977 1452 1185 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5275 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2733 2893 3343 4137 4157 2733 Size: 160 450 794 20 4403 Fragments arranged by size: 4403 794 450 160 20 ClaI AT'CG_AT Cuts at: 1554 2412 1554 Size: 858 4969 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2056 2091 529 Size: 35 1492 35 4265 Fragments arranged by size: 4265 1492 35 35 DraI TTT'AAA Cuts at: 2451 3131 2451 Size: 680 5147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 3274 4163 Size: 54 1425 50 275 18 426 1018 889 Cuts at: 4163 5792 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1018 889 426 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2708 2771 3757 1353 Size: 1355 63 986 3423 Fragments arranged by size: 3423 1355 986 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2397 4414 4788 4934 387 Size: 1064 138 808 2017 374 146 1280 Fragments arranged by size: 2017 1280 1064 808 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 3092 3117 3890 4487 5274 5542 Size: 393 611 849 25 773 597 787 268 Cuts at: 5542 1239 Size: 1524 Fragments arranged by size: 1524 849 787 773 611 597 393 268 25 EcoO109I rG'GnC_Cy Cuts at: 2127 2127 Size: 5827 EcoRI G'AATT_C Cuts at: 2813 3136 3514 2813 Size: 323 378 5126 Fragments arranged by size: 5126 378 323 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3122 3122 Size: 5827 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 4974 1678 Size: 3296 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2863 2895 4468 4500 4542 Size: 32 135 32 1291 32 1573 32 42 Cuts at: 4542 4574 1373 Size: 32 2626 Fragments arranged by size: 2626 1573 1291 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2849 3171 3417 669 Size: 988 281 911 322 246 3079 Fragments arranged by size: 3079 988 911 322 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KpnI G_GTAC'C Cuts at: 3152 3152 Size: 5827 MfeI C'AATT_G Cuts at: 3081 3426 3081 Size: 345 5482 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4941 5125 1305 Size: 3636 184 2007 Fragments arranged by size: 3636 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2258 10 Size: 54 1425 343 426 3579 Fragments arranged by size: 3579 1425 426 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2593 2615 2747 2867 3785 4144 4303 Size: 648 1427 22 132 120 918 359 159 Cuts at: 4303 518 Size: 2042 Fragments arranged by size: 2042 1427 918 648 359 159 132 120 22 MspA1I CmG'CkG Cuts at: 1606 2647 3243 3920 5068 5313 1606 Size: 1041 596 677 1148 245 2120 Fragments arranged by size: 2120 1148 1041 677 596 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5275 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 2188 3185 4730 1132 Size: 97 959 997 1545 2229 Fragments arranged by size: 2229 1545 997 959 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5827 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 2999 1108 Size: 1891 3936 PfoI T'CCnGG_A Cuts at: 2808 3007 2808 Size: 199 5628 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspOMI G'GGCC_C Cuts at: 2127 2127 Size: 5827 PstI C_TGCA'G Cuts at: 830 3179 830 Size: 2349 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SexAI A'CCwGG_T Cuts at: 2495 2495 Size: 5827 SfcI C'TryA_G Cuts at: 826 1045 2968 3175 4310 4991 5182 826 Size: 219 1923 207 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 219 207 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 3154 1872 Size: 1282 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3931 4832 5094 820 Size: 197 926 1988 901 262 1553 Fragments arranged by size: 1988 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SspI AAT'ATT Cuts at: 5 52 2785 3751 5 Size: 47 2733 966 2081 Fragments arranged by size: 2733 2081 966 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5827 StyI C'CwwG_G Cuts at: 513 1065 3266 513 Size: 552 2201 3074 Fragments arranged by size: 3074 2201 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3854 3871 4024 4209 4654 325 Size: 1255 2274 17 153 185 445 1498 Fragments arranged by size: 2274 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4073 5785 96 Size: 274 80 33 51 1443 2096 1712 138 Fragments arranged by size: 2096 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3015 3036 3047 3068 5787 5819 3015 Size: 21 11 21 2719 32 3023 Fragments arranged by size: 3023 2719 32 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3152 1870 Size: 1282 4545 XmnI GAAnn'nnTTC Cuts at: 2603 2804 3956 2603 Size: 201 1152 4474 Fragments arranged by size: 4474 1152 201 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3635 276 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI