(Circular) (Six-base) MAPSORT of: pFN21AB8965.seq Check: 7015 from: 1 to: 5767 pFN21AB8965 5767 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2583 2583 Size: 5767 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2715 3110 1937 Size: 778 395 4594 Fragments arranged by size: 4594 778 395 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3830 5214 5482 1632 Size: 2198 1384 268 1917 Fragments arranged by size: 2198 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 4666 5643 1203 Size: 3463 977 1327 Fragments arranged by size: 3463 1327 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 2014 4496 2014 Size: 2482 3285 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2567 1184 Size: 1383 4384 AlwNI CAG_nnn'CTG Cuts at: 2161 2797 3510 5082 2161 Size: 636 713 1572 2846 Fragments arranged by size: 2846 1572 713 636 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 2719 3092 1870 Size: 73 776 373 4545 Fragments arranged by size: 4545 776 373 73 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2061 2566 2656 2684 2771 Size: 325 217 627 274 505 90 28 87 Cuts at: 2771 3088 4543 618 Size: 317 1455 1842 Fragments arranged by size: 1842 1455 627 505 325 317 274 217 90 87 28 BanII G_rGCy'C Cuts at: 1391 2192 2336 2412 2453 2726 3017 3086 1391 Size: 801 144 76 41 273 291 69 4072 Fragments arranged by size: 4072 801 291 273 144 76 69 41 BbeI G_GCGC'C Cuts at: 2065 2688 2775 2065 Size: 623 87 5057 Fragments arranged by size: 5057 623 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 2956 928 Size: 971 149 908 3739 Fragments arranged by size: 3739 971 908 149 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2352 2386 3958 3992 1903 Size: 34 415 34 1572 34 3678 Fragments arranged by size: 3678 1572 415 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3659 4869 3659 Size: 1210 4557 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4378 136 Size: 107 122 71 631 605 2706 1525 Fragments arranged by size: 2706 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2148 5762 2148 Size: 3614 2153 BlpI GC'TnA_GC Cuts at: 2141 3184 2141 Size: 1043 4724 Bme1580I G_kGCm'C Cuts at: 1790 2474 2571 2661 3828 4984 1790 Size: 684 97 90 1167 1156 2573 Fragments arranged by size: 2573 1167 1156 684 97 90 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3498 4456 442 Size: 416 53 83 399 24 2081 958 1753 Fragments arranged by size: 2081 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2350 2350 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2513 2876 4427 1239 Size: 1274 363 1551 2579 Fragments arranged by size: 2579 1551 1274 363 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2366 3892 4757 5055 2366 Size: 1526 865 298 3078 Fragments arranged by size: 3078 1526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4430 882 Size: 617 2931 2219 Fragments arranged by size: 2931 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 5761 5761 Size: 5767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2062 2685 2750 2772 Size: 53 83 186 936 529 623 65 22 Cuts at: 2772 2984 3574 3956 4591 275 Size: 212 590 382 635 1451 Fragments arranged by size: 1451 936 635 623 590 529 382 212 186 83 65 53 22 BsaWI w'CCGG_w Cuts at: 4200 4598 4872 5019 5430 4200 Size: 398 274 147 411 4537 Fragments arranged by size: 4537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2849 2879 1178 Size: 30 165 30 1446 30 4066 Fragments arranged by size: 4066 1446 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 1186 Size: 1156 4611 BseYI C'CCAG_C Cuts at: 2555 2893 2936 4970 5466 2555 Size: 338 43 2034 496 2856 Fragments arranged by size: 2856 2034 496 338 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2855 1163 Size: 573 1119 4075 Fragments arranged by size: 4075 1119 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2351 2457 2628 3006 3217 3978 Size: 275 186 350 106 171 378 211 761 Cuts at: 3978 5006 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 378 350 275 211 186 171 106 BsiHKAI G_wGCw'C Cuts at: 3828 3913 4984 3828 Size: 85 1071 4611 Fragments arranged by size: 4611 1071 85 BsmI GAATG_Cn' Cuts at: 3278 3371 3278 Size: 93 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2192 2336 2412 2453 2474 2571 2661 Size: 399 402 144 76 41 21 97 90 Cuts at: 2661 2726 3017 3086 3828 3913 4984 1391 Size: 65 291 69 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 402 399 291 144 97 90 85 76 69 65 41 21 BspEI T'CCGG_A Cuts at: 5430 5430 Size: 5767 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2583 3022 3122 844 Size: 1739 439 100 3489 Fragments arranged by size: 3489 1739 439 100 BsrBI CCG'CTC Cuts at: 1394 1823 3654 1394 Size: 429 1831 3507 Fragments arranged by size: 3507 1831 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4262 4436 65 Size: 1183 3014 174 1396 Fragments arranged by size: 3014 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 2456 2627 2681 2687 3005 4411 Size: 110 241 405 171 54 6 318 1406 Cuts at: 4411 4598 1700 Size: 187 2869 Fragments arranged by size: 2869 1406 405 318 241 187 171 110 54 6 BsrGI T'GTAC_A Cuts at: 96 2391 96 Size: 2295 3472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 2467 3827 4839 2467 Size: 1360 1012 3395 Fragments arranged by size: 3395 1360 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5767 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 3034 1184 Size: 1850 3917 BstXI CCAn_nnnn'nTGG Cuts at: 2485 2485 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2148 3097 3464 3849 3866 5318 5404 5416 Size: 176 949 367 385 17 1452 86 12 Cuts at: 5416 5762 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 949 385 367 346 176 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2754 2814 2915 2979 513 Size: 552 939 750 60 101 64 3301 Fragments arranged by size: 3301 939 750 552 101 64 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2074 487 Size: 683 56 848 4180 Fragments arranged by size: 4180 848 683 56 BtsI GCAGTG_nn' Cuts at: 3283 4077 4097 3283 Size: 794 20 4953 Fragments arranged by size: 4953 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DraIII CAC_nnn'GTG Cuts at: 2566 2566 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2307 2348 Size: 54 1425 50 275 18 276 201 41 Cuts at: 2348 2454 2518 2590 2625 2738 2817 3003 3214 Size: 106 64 72 35 113 79 186 211 Cuts at: 3214 4103 5732 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 276 275 211 201 186 113 106 79 72 64 54 50 43 41 35 18 EagI C'GGCC_G Cuts at: 1537 1812 2348 2454 2625 3003 3214 1537 Size: 275 536 106 171 378 211 4090 Fragments arranged by size: 4090 536 378 275 211 171 106 EarI CTCTTCn'nnn_ Cuts at: 1353 2995 3697 1353 Size: 1642 702 3423 Fragments arranged by size: 3423 1642 702 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4354 4728 4874 387 Size: 1064 138 2765 374 146 1280 Fragments arranged by size: 2765 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2876 3830 4427 5214 5482 1239 Size: 393 881 363 954 597 787 268 1524 Fragments arranged by size: 1524 954 881 787 597 393 363 268 EcoO109I rG'GnC_Cy Cuts at: 2263 2263 Size: 5767 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 2065 2134 2381 2492 2688 2762 2775 4914 Size: 387 69 247 111 196 74 13 2139 Cuts at: 4914 1678 Size: 2531 Fragments arranged by size: 2531 2139 387 247 196 111 74 69 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 4408 4440 4482 Size: 32 135 32 428 32 2376 32 42 Cuts at: 4482 4514 1373 Size: 32 2626 Fragments arranged by size: 2626 2376 428 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KasI G'GCGC_C Cuts at: 2061 2684 2771 2061 Size: 623 87 5057 Fragments arranged by size: 5057 623 87 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 4881 5065 1305 Size: 1302 2274 184 2007 Fragments arranged by size: 2274 2007 1302 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 10 Size: 54 1425 343 276 3669 Fragments arranged by size: 3669 1425 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 3725 4084 4243 518 Size: 648 936 1623 359 159 2042 Fragments arranged by size: 2042 1623 936 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2671 2756 2769 2816 2917 3183 3860 5008 Size: 1065 85 13 47 101 266 677 1148 Cuts at: 5008 5253 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1065 677 266 245 101 85 47 13 NaeI GCC'GGC Cuts at: 2053 2458 2629 3007 2053 Size: 405 171 378 4813 Fragments arranged by size: 4813 405 378 171 NarI GG'CG_CC Cuts at: 2062 2685 2772 2062 Size: 623 87 5057 Fragments arranged by size: 5057 623 87 NcoI C'CATG_G Cuts at: 513 1065 2004 2979 513 Size: 552 939 975 3301 Fragments arranged by size: 3301 975 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NgoMIV G'CCGG_C Cuts at: 2051 2456 2627 3005 2051 Size: 405 171 378 4813 Fragments arranged by size: 4813 405 378 171 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 2348 3214 2348 Size: 866 4901 NspI r_CATG'y Cuts at: 1132 1229 2944 3125 4670 1132 Size: 97 1715 181 1545 2229 Fragments arranged by size: 2229 1715 1545 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5767 PfoI T'CCnGG_A Cuts at: 2427 2427 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2306 2338 3836 3868 5349 5381 2306 Size: 32 1498 32 1481 32 2692 Fragments arranged by size: 2692 1498 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PstI C_TGCA'G Cuts at: 830 2166 3119 830 Size: 1336 953 3478 Fragments arranged by size: 3478 1336 953 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 SacII CC_GC'GG Cuts at: 2757 2817 2918 2757 Size: 60 101 5606 Fragments arranged by size: 5606 101 60 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 2162 3115 4250 4931 5122 826 Size: 219 1117 953 1135 681 191 1471 Fragments arranged by size: 1471 1135 1117 953 681 219 191 SfoI GGC'GCC Cuts at: 2063 2686 2773 2063 Size: 623 87 5057 Fragments arranged by size: 5057 623 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 2681 2687 1700 Size: 981 6 4780 Fragments arranged by size: 4780 981 6 SmaI CCC'GGG Cuts at: 1872 3094 1872 Size: 1222 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2381 3871 4772 5034 820 Size: 197 926 438 1490 901 262 1553 Fragments arranged by size: 1553 1490 926 901 438 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5767 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2259 2266 2979 3206 513 Size: 552 939 27 228 7 713 227 3074 Fragments arranged by size: 3074 939 713 552 228 227 27 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 2964 3794 3811 3964 4149 4594 Size: 1255 557 827 830 17 153 185 445 Cuts at: 4594 325 Size: 1498 Fragments arranged by size: 1498 1255 830 827 557 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 4013 5725 Size: 274 80 33 51 1443 414 1622 1712 Cuts at: 5725 96 Size: 138 Fragments arranged by size: 1712 1622 1443 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2564 2596 5727 5759 2564 Size: 32 3131 32 2572 Fragments arranged by size: 3131 2572 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2851 2851 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5767 XmaI C'CCGG_G Cuts at: 1870 3092 1870 Size: 1222 4545 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI