(Circular) (Six-base) MAPSORT of: pFN21AB8964.seq Check: 7578 from: 1 to: 5755 pFN21AB8964 5755 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2221 2221 Size: 5755 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2076 3012 3098 1937 Size: 139 936 86 4594 Fragments arranged by size: 4594 936 139 86 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2900 3012 3818 5202 5470 1632 Size: 1268 112 806 1384 268 1917 Fragments arranged by size: 1917 1384 1268 806 268 112 AfeI AGC'GCT Cuts at: 2483 2483 Size: 5755 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 2601 4654 5631 1203 Size: 1398 2053 977 1327 Fragments arranged by size: 2053 1398 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 2213 2711 2777 3498 5070 2213 Size: 498 66 721 1572 2898 Fragments arranged by size: 2898 1572 721 498 66 ApaI G_GGCC'C Cuts at: 2232 2287 2581 2232 Size: 55 294 5406 Fragments arranged by size: 5406 294 55 ApaLI G'TGCA_C Cuts at: 2303 3812 4968 2303 Size: 1509 1156 3090 Fragments arranged by size: 3090 1509 1156 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2287 3080 1870 Size: 73 344 793 4545 Fragments arranged by size: 4545 793 344 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2200 2401 3076 4531 618 Size: 325 217 627 413 201 675 1455 1842 Fragments arranged by size: 1842 1455 675 627 413 325 217 201 BanII G_rGCy'C Cuts at: 1391 2015 2169 2232 2287 2570 2581 2815 2868 Size: 624 154 63 55 283 11 234 53 Cuts at: 2868 3074 1391 Size: 206 4072 Fragments arranged by size: 4072 624 283 234 206 154 63 55 53 11 BbeI G_GCGC'C Cuts at: 2405 2405 Size: 5755 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4784 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3946 3980 1903 Size: 34 50 34 1925 34 3678 Fragments arranged by size: 3678 1925 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BfrBI ATG'CAT Cuts at: 3050 3050 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2100 2232 2287 2307 2581 3816 4972 1790 Size: 310 132 55 20 274 1235 1156 2573 Fragments arranged by size: 2573 1235 1156 310 274 132 55 20 BmgBI CAC'GTC Cuts at: 1318 2968 1318 Size: 1650 4105 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2087 2584 3486 Size: 416 53 83 399 24 670 497 902 Cuts at: 3486 4444 442 Size: 958 1753 Fragments arranged by size: 1753 958 902 670 497 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 2754 2882 3027 4415 1239 Size: 1430 85 128 145 1388 2579 Fragments arranged by size: 2579 1430 1388 145 128 85 Bpu10I CC'TnA_GC Cuts at: 2593 2778 2593 Size: 185 5570 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2443 3880 4745 5043 2443 Size: 1437 865 298 3155 Fragments arranged by size: 3155 1437 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2402 3562 3944 4579 Size: 53 83 186 936 869 1160 382 635 Cuts at: 4579 275 Size: 1451 Fragments arranged by size: 1451 1160 936 869 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 1178 Size: 30 165 30 1497 30 4003 Fragments arranged by size: 4003 1497 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 1186 Size: 1480 4275 BseYI C'CCAG_C Cuts at: 2008 2206 2588 2757 4958 5454 2008 Size: 198 382 169 2201 496 2309 Fragments arranged by size: 2309 2201 496 382 198 169 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 2868 3816 3901 4972 2307 Size: 561 948 85 1071 3090 Fragments arranged by size: 3090 1071 948 561 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2100 2169 2232 2287 2307 2570 Size: 399 225 85 69 63 55 20 263 Cuts at: 2570 2581 2815 2868 3074 3816 3901 4972 1391 Size: 11 234 53 206 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 263 234 225 206 85 85 69 63 55 53 20 11 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2221 2631 3110 844 Size: 1377 410 479 3489 Fragments arranged by size: 3489 1377 479 410 BsrBI CCG'CTC Cuts at: 1394 1823 3036 3642 1394 Size: 429 1213 606 3507 Fragments arranged by size: 3507 1213 606 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 4399 4586 1700 Size: 110 273 2316 187 2869 Fragments arranged by size: 2869 2316 273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 2086 1184 Size: 902 4853 BstYI r'GATC_y Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750 Size: 1113 367 385 17 1452 86 12 346 Cuts at: 5750 1972 Size: 1977 Fragments arranged by size: 1977 1452 1113 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2188 2784 2188 Size: 596 5159 BtgI C'CryG_G Cuts at: 513 1065 2103 2385 2733 513 Size: 552 1038 282 348 3535 Fragments arranged by size: 3535 1038 552 348 282 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 2180 3271 4065 4085 2180 Size: 1091 794 20 3850 Fragments arranged by size: 3850 1091 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2528 4762 809 Size: 507 1212 2234 1802 Fragments arranged by size: 2234 1802 1212 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2388 2736 Size: 54 1425 50 275 18 210 348 348 Cuts at: 2736 3202 4091 5720 8 Size: 466 889 1629 43 Fragments arranged by size: 1629 1425 889 466 348 348 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 3685 1353 Size: 1106 1226 3423 Fragments arranged by size: 3423 1226 1106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2866 2866 Size: 5755 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2669 2754 2882 2900 3012 3027 3818 Size: 393 1037 85 128 18 112 15 791 Cuts at: 3818 4415 5202 5470 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1037 791 787 597 393 268 128 112 85 18 15 EcoO109I rG'GnC_Cy Cuts at: 2192 2228 2283 2284 2291 2578 3022 2192 Size: 36 55 1 7 287 444 4925 Fragments arranged by size: 4925 444 287 55 36 7 1 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2405 2485 4902 1678 Size: 727 80 2417 2531 Fragments arranged by size: 2531 2417 727 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2119 2151 2810 2842 4396 Size: 32 135 32 547 32 659 32 1554 Cuts at: 4396 4428 4470 4502 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1554 659 547 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KasI G'GCGC_C Cuts at: 2401 2401 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2390 10 Size: 54 1425 343 558 3375 Fragments arranged by size: 3375 1425 558 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 3713 4072 4231 518 Size: 648 1218 1329 359 159 2042 Fragments arranged by size: 2042 1329 1218 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2256 2676 2735 3171 3848 4996 5241 1606 Size: 650 420 59 436 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 650 436 420 245 59 NarI GG'CG_CC Cuts at: 2402 2402 Size: 5755 NcoI C'CATG_G Cuts at: 513 1065 2103 2385 513 Size: 552 1038 282 3883 Fragments arranged by size: 3883 1038 552 282 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NsiI A_TGCA'T Cuts at: 3052 3052 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2605 3113 4658 1132 Size: 97 1376 508 1545 2229 Fragments arranged by size: 2229 1545 1376 508 97 PasI CC'CwG_GG Cuts at: 1348 2159 2224 2791 2870 1348 Size: 811 65 567 79 4233 Fragments arranged by size: 4233 811 567 79 65 PciI A'CATG_T Cuts at: 2601 4654 2601 Size: 2053 3702 PflMI CCAn_nnn'nTGG Cuts at: 1108 2038 1108 Size: 930 4825 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2192 2291 2192 Size: 99 5656 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PspOMI G'GGCC_C Cuts at: 2228 2283 2577 2228 Size: 55 294 5406 Fragments arranged by size: 5406 294 55 PstI C_TGCA'G Cuts at: 830 2218 3107 830 Size: 1388 889 3478 Fragments arranged by size: 3478 1388 889 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SacI G_AGCT'C Cuts at: 2868 2868 Size: 5755 SacII CC_GC'GG Cuts at: 2736 2736 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2214 3103 4238 4919 5110 826 Size: 219 1169 889 1135 681 191 1471 Fragments arranged by size: 1471 1169 1135 889 681 219 191 SfoI GGC'GCC Cuts at: 2403 2403 Size: 5755 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 2083 1700 Size: 383 5372 SmaI CCC'GGG Cuts at: 1872 2289 3082 1872 Size: 417 793 4545 Fragments arranged by size: 4545 793 417 SmlI C'TyrA_G Cuts at: 820 1017 1943 2422 3859 4760 5022 820 Size: 197 926 479 1437 901 262 1553 Fragments arranged by size: 1553 1437 926 901 479 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 2244 1517 Size: 727 5028 StyI C'CwwG_G Cuts at: 513 1065 2103 2385 3194 513 Size: 552 1038 282 809 3074 Fragments arranged by size: 3074 1038 809 552 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 3782 3799 3952 4137 4582 325 Size: 1255 657 1545 17 153 185 445 1498 Fragments arranged by size: 1545 1498 1255 657 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2900 2932 5715 5747 2900 Size: 32 2783 32 2908 Fragments arranged by size: 2908 2783 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2707 1316 Size: 1391 4364 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2965 2965 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 2287 3080 1870 Size: 417 793 4545 Fragments arranged by size: 4545 793 417 XmnI GAAnn'nnTTC Cuts at: 2639 3884 2639 Size: 1245 4510 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI