(Circular) (Six-base) MAPSORT of: pFN21AB8962.seq Check: 764 from: 1 to: 5731 pFN21AB8962 5731 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3074 1937 Size: 1137 4594 Acc65I G'GTAC_C Cuts at: 2431 3052 2431 Size: 621 5110 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3794 5178 5446 1632 Size: 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 268 AfeI AGC'GCT Cuts at: 2075 2075 Size: 5731 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 4630 5607 1203 Size: 3427 977 1327 Fragments arranged by size: 3427 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2117 1184 Size: 933 4798 AlwNI CAG_nnn'CTG Cuts at: 2662 2845 3474 5046 2662 Size: 183 629 1572 3347 Fragments arranged by size: 3347 1572 629 183 ApaI G_GGCC'C Cuts at: 2672 2672 Size: 5731 ApaLI G'TGCA_C Cuts at: 3788 4944 3788 Size: 1156 4575 ApoI r'AATT_y Cuts at: 1493 2861 3040 3266 3418 5527 1493 Size: 1368 179 226 152 2109 1697 Fragments arranged by size: 2109 1697 1368 226 179 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2375 2461 3056 1870 Size: 73 432 86 595 4545 Fragments arranged by size: 4545 595 432 86 73 BamHI G'GATC_C Cuts at: 2997 3061 3428 2997 Size: 64 367 5300 Fragments arranged by size: 5300 367 64 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2431 2820 3052 4507 618 Size: 325 217 627 644 389 232 1455 1842 Fragments arranged by size: 1842 1455 644 627 389 325 232 217 BanII G_rGCy'C Cuts at: 1391 2049 2151 2672 2684 3050 1391 Size: 658 102 521 12 366 4072 Fragments arranged by size: 4072 658 521 366 102 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4760 BbvCI CC'TCA_GC Cuts at: 2574 2574 Size: 5731 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3922 3956 1903 Size: 34 1985 34 3678 Fragments arranged by size: 3678 1985 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2667 4342 136 Size: 107 122 71 631 605 995 1675 1525 Fragments arranged by size: 1675 1525 995 631 605 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 3148 3148 Size: 5731 Bme1580I G_kGCm'C Cuts at: 1790 2442 2672 2825 3792 4948 1790 Size: 652 230 153 967 1156 2573 Fragments arranged by size: 2573 1156 967 652 230 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3462 4420 442 Size: 416 53 83 399 24 2045 958 1753 Fragments arranged by size: 2045 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2707 2921 4391 1239 Size: 1468 214 1470 2579 Fragments arranged by size: 2579 1470 1468 214 Bpu10I CC'TnA_GC Cuts at: 2574 2574 Size: 5731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2957 3856 4721 5019 2957 Size: 899 865 298 3669 Fragments arranged by size: 3669 899 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2597 4394 882 Size: 617 1098 1797 2219 Fragments arranged by size: 2219 1797 1098 617 BsaAI yAC'GTr Cuts at: 493 1204 3522 493 Size: 711 2318 2702 Fragments arranged by size: 2702 2318 711 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2486 3538 3920 4555 Size: 53 83 186 936 953 1052 382 635 Cuts at: 4555 275 Size: 1451 Fragments arranged by size: 1451 1052 953 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4164 4562 4836 4983 5394 4164 Size: 398 274 147 411 4501 Fragments arranged by size: 4501 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5506 Fragments arranged by size: 5506 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2082 2339 2771 1186 Size: 896 257 432 4146 Fragments arranged by size: 4146 896 432 257 BseYI C'CCAG_C Cuts at: 2189 2441 2578 2630 4934 5430 2189 Size: 252 137 52 2304 496 2490 Fragments arranged by size: 2490 2304 496 252 137 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3181 3942 4970 1540 Size: 275 186 1180 761 1028 2301 Fragments arranged by size: 2301 1180 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 3792 3877 4948 2427 Size: 1365 85 1071 3210 Fragments arranged by size: 3210 1365 1071 85 BsiWI C'GTAC_G Cuts at: 2037 2037 Size: 5731 BsmI GAATG_Cn' Cuts at: 3242 3335 3242 Size: 93 5638 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2151 2427 2442 2672 2684 2825 Size: 399 259 102 276 15 230 12 141 Cuts at: 2825 3050 3792 3877 4948 1391 Size: 225 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 259 230 225 141 102 85 15 12 BspEI T'CCGG_A Cuts at: 5394 5394 Size: 5731 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3086 844 Size: 2242 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2805 3618 1394 Size: 429 982 813 3507 Fragments arranged by size: 3507 982 813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2026 4226 4400 65 Size: 1183 778 2200 174 1396 Fragments arranged by size: 2200 1396 1183 778 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4375 4562 1700 Size: 110 2565 187 2869 Fragments arranged by size: 2869 2565 187 110 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 3484 BssHII G'CGCG_C Cuts at: 1925 2551 1925 Size: 626 5105 BssSI C'ACGA_G Cuts at: 2426 3791 4803 2426 Size: 1365 1012 3354 Fragments arranged by size: 3354 1365 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 2514 4535 2514 Size: 2021 3710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstXI CCAn_nnnn'nTGG Cuts at: 2498 2498 Size: 5731 BstYI r'GATC_y Cuts at: 1972 2997 3061 3428 3813 3830 5282 5368 5380 Size: 1025 64 367 385 17 1452 86 12 Cuts at: 5380 5726 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1025 385 367 346 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4992 Fragments arranged by size: 4992 683 56 BtsI GCAGTG_nn' Cuts at: 3247 4041 4061 3247 Size: 794 20 4917 Fragments arranged by size: 4917 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DraIII CAC_nnn'GTG Cuts at: 2315 2315 Size: 5731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2611 2936 4738 809 Size: 507 1295 325 1802 1802 Fragments arranged by size: 1802 1802 1295 507 325 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696 Size: 54 1425 50 275 18 1348 889 1629 Cuts at: 5696 8 Size: 43 Fragments arranged by size: 1629 1425 1348 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2810 3661 1353 Size: 1457 851 3423 Fragments arranged by size: 3423 1457 851 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 4318 4692 4838 387 Size: 1064 138 490 2239 374 146 1280 Fragments arranged by size: 2239 1280 1064 490 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2707 2921 3794 4391 5178 5446 1239 Size: 393 1075 214 873 597 787 268 1524 Fragments arranged by size: 1524 1075 873 787 597 393 268 214 EcoO109I rG'GnC_Cy Cuts at: 2669 2669 Size: 5731 EcoRI G'AATT_C Cuts at: 3040 3418 3040 Size: 378 5353 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 2077 2896 4878 1678 Size: 399 819 1982 2531 Fragments arranged by size: 2531 1982 819 399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 4372 4404 4446 Size: 32 135 32 427 32 2341 32 42 Cuts at: 4446 4478 1373 Size: 32 2626 Fragments arranged by size: 2626 2341 427 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KpnI G_GTAC'C Cuts at: 2435 3056 2435 Size: 621 5110 MfeI C'AATT_G Cuts at: 2122 3330 2122 Size: 1208 4523 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2640 4845 5029 1305 Size: 1335 2205 184 2007 Fragments arranged by size: 2205 2007 1335 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3909 Fragments arranged by size: 3909 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 3689 4048 4207 518 Size: 648 933 1590 359 159 2042 Fragments arranged by size: 2042 1590 933 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2230 2290 2533 2578 3147 3824 4972 5217 Size: 624 60 243 45 569 677 1148 245 Cuts at: 5217 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 624 569 245 243 60 45 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5179 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2013 2098 2344 3089 4634 1132 Size: 97 784 85 246 745 1545 2229 Fragments arranged by size: 2229 1545 784 745 246 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 2845 1108 Size: 1737 3994 PfoI T'CCnGG_A Cuts at: 3000 3000 Size: 5731 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PspOMI G'GGCC_C Cuts at: 2668 2668 Size: 5731 PstI C_TGCA'G Cuts at: 830 3083 830 Size: 2253 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 2230 2578 2230 Size: 348 5383 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 2077 3079 4214 4895 5086 826 Size: 219 1032 1002 1135 681 191 1471 Fragments arranged by size: 1471 1135 1032 1002 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 3058 1872 Size: 1186 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2972 3835 4736 4998 820 Size: 197 926 1029 863 901 262 1553 Fragments arranged by size: 1553 1029 926 901 863 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 3089 1132 Size: 1957 3774 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5731 StyI C'CwwG_G Cuts at: 513 1065 2049 2416 3170 513 Size: 552 984 367 754 3074 Fragments arranged by size: 3074 984 754 552 367 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3758 3775 3928 4113 4558 325 Size: 1255 2178 17 153 185 445 1498 Fragments arranged by size: 2178 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 3977 5689 Size: 274 80 33 51 1443 366 1634 1712 Cuts at: 5689 96 Size: 138 Fragments arranged by size: 1712 1634 1443 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5691 5723 5691 Size: 32 5699 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 3056 1870 Size: 1186 4545 XmnI GAAnn'nnTTC Cuts at: 3860 3860 Size: 5731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI